Spaceflight missions must limit biological contamination on both the outbound and return legs to comply with planetary protection requirements. Depending on the mission profile, contamination concerns may include the potential presence of bioactive molecules as defined by NASA’s Planetary Protection policies. Thus, the present study has examined the temperature and time requirements for sufficient inactivation/degradation of an infectious, heat-stable prion protein (Sup35NM), which serves as a model bioactive molecule. Bovine serum albumin was used to establish the method parameters and feasibility. Differential scanning calorimetry, Fourier transform infrared spectroscopy, analytical reversed-phase high-performance liquid chromatography, and mass spectrometry were utilized to analyze heat-treated samples, with non-treated samples serving as controls. Heat treatment at 400°C for 5 seconds was found to result in substantial decomposition of Sup35NM. In addition to the disruption of the protein backbone amide bonds, the side chain residues were also compromised. Fragments of molecular weight <4600 were observed by mass spectrometry but the impact of treatment on both the backbone and side chains of Sup35NM suggested that these fragments would not self-associate to create potentially pathogenic entities. The present methodology provided insight into the protein degradation process and can be applied to a variety of treatment strategies (e.g., any form of sterilization or inactivation) to ensure a lack of protein-based contamination of isolated extraterrestrial specimens.

Fortified Island (FORTIS) examines Iron Age fortifications on the island of Bornholm to assess their characters, locations and chronologies. Through a multimethod approach, the project deepens our understanding of fortifications in relation to their physical and cultural landscapes, both on Bornholm and in the Baltic Sea Region more generally.

This article explores and discusses the change processes and pedagogical dilemmas ignited by introducing wild pedagogies to pedagogical employees in Danish early childhood institutions. By analysing experiments aimed at developing new play and learning environments, carried out as part of a large design-based research project, we discuss how existing “roots” of early childhood education in Denmark provide a fertile soil for the introduction of wild pedagogies. We identify two “shoots of change” with a potential for pushing the status quo in relations between children, adults, and more-than-human nature. Centreing on altering the place of nature in early childhood education and carving out time for more open approaches, these shoots are in close dialogue with wild pedagogies. By experimenting with these shoots of change, pedagogical dilemmas became more visible, important, and present to the participants. Attending to and exploring such dilemmas are crucial aspects of keeping socio-cultural change processes in motion.

Due to the provisions of the Svalbard Treaty, Russia has kept a presence on this Norwegian archipelago – primarily based on coal mining – and has regularly made it clear that ensuring the continuation of this presence is a political goal. Since the late 2000s, Russia has attempted to revitalise its presence, stressing the need for economic efficiency and diversification away from coal. This includes tourism, fish processing and research activities. In recent years, Russia’s official rhetoric on Svalbard has sharpened, i.a. accusing Norway of breaching the treaty’s provisions on military use of the islands. The article contrasts the statements with the concrete actions undertaken by Russia to preserve and develop its presence. Russia’s policy of presence on Svalbard is not particularly well-coordinated or strategic – beyond an increasing openness to exploring new ways to sustain a sufficient presence. Financial limitations have constrained initiatives. The search for new activities and solutions is driven primarily by the need for cost-cutting and consolidating a limited presence deemed necessary for Russian security interest, not as strategies aimed at increasing Russian influence over the archipelago.

In July 2022, a genetically linked and geographically dispersed cluster of 12 cases of Shiga toxin-producing Escherichia coli (STEC) O103:H2 was detected by the UK Health Security Agency using whole genome sequencing. Review of food history questionnaires identified cheese (particularly an unpasteurized brie-style cheese) and mixed salad leaves as potential vehicles. A case–control study was conducted to investigate exposure to these products. Case food history information was collected by telephone. Controls were recruited using a market research panel and self-completed an online questionnaire. Univariable and multivariable analyses were undertaken using Firth Logistic Regression. Eleven cases and 24 controls were included in the analysis. Consumption of the brie-style cheese of interest was associated with illness (OR 57.5, 95% confidence interval: 3.10–1,060). Concurrently, the production of the brie-style cheese was investigated. Microbiological sample results for the cheese products and implicated dairy herd did not identify the outbreak strain, but did identify the presence of stx genes and STEC, respectively. Together, epidemiological, microbiological, and environmental investigations provided evidence that the brie-style cheese was the vehicle for this outbreak. Production of unpasteurized dairy products was suspended by the business operator, and a review of practices was performed.

Latent class regression models relate covariates and latent constructs such as psychiatric disorders. Though full maximum likelihood estimation is available, estimation is often in three steps: (i) a latent class model is fitted without covariates; (ii) latent class scores are predicted; and (iii) the scores are regressed on covariates. We propose a new method for predicting class scores that, in contrast to posterior probability-based methods, yields consistent estimators of the parameters in the third step. Additionally, in simulation studies the new methodology exhibited only a minor loss of efficiency. Finally, the new and the posterior probability-based methods are compared in an analysis of mobility/exercise.

Many studies in fields such as psychology and educational sciences obtain information about attributes of subjects through observational studies, in which raters score subjects using multiple-item rating scales. Error variance due to measurement effects, such as items and raters, attenuate the regression coefficients and lower the power of (hierarchical) linear models. A modeling procedure is discussed to reduce the attenuation. The procedure consists of (1) an item response theory (IRT) model to map the discrete item responses to a continuous latent scale and (2) a generalizability theory (GT) model to separate the variance in the latent measurement into variance components of interest and nuisance variance components. It will be shown how measurements obtained from this mixture of IRT and GT models can be embedded in (hierarchical) linear models, both as predictor or criterion variables, such that error variance due to nuisance effects are partialled out. Using examples from the field of educational measurement, it is shown how general-purpose software can be used to implement the modeling procedure.

The aim of this randomised controlled trial was to investigate the effects of breakfast high or low in protein on body composition and cardiometabolic markers in young women with overweight. In total, fifty-six women aged 18–30 years consumed a breakfast containing either high protein (34 g protein, n 26) or low protein (6 g protein, n 30) for 12 weeks. Measurements of body composition by dual-energy X-ray absorptiometry, waist circumference, glucose tolerance, fasting glucose, insulin and lipid profile were performed before and after this period. The primary outcome was fat mass. Satiety and hunger were evaluated by self-reported Visual Analogue Scale (VAS) scores. Dietary intake was estimated by 4-d dietary records, and calcium intake was estimated by FFQ. At baseline, relative daily protein intake was 15·2 ± 2·8 E%, which increased to 19·3 ± 3·4 E% in high protein but was unchanged in low protein (P < 0·001 between groups). High protein reported higher satiety compared with low protein (P = 0·02). Yet, no group differences were observed in changes in energy intake, body composition, blood lipid profile or measures of glucose tolerance (all P > 0·10). However, bone mineral content tended to increase in high protein (P = 0·05) and decrease in low protein (P = 0·07, interaction effect: P = 0·01). Conclusively, a high v. low content of protein in breakfast increased satiety but did not affect body composition or cardiometabolic markers in young women with overweight. This study adds to the sparse evidence on the effects of breakfast with different macronutrient compositions on health parameters in women with overweight. Registered at clinicaltrials.gov: NCT04518605.

Declining labor force participation of older men throughout the 20th century and recent increases in participation have generated substantial interest in understanding the effect of public pensions on retirement. The National Bureau of Economic Research's International Social Security (ISS) Project, a long-term collaboration among researchers in a dozen developed countries, has explored this and related questions. The project employs a harmonized approach to conduct within-country analyses that are combined for meaningful cross-country comparisons. The key lesson is that the choices of policy makers affect the incentive to work at older ages and these incentives have important effects on retirement behavior.

This paper examines the role of remanufacturing in healthcare as a key circular design strategy, particularly for medical devices, assessing its socio-technical, environmental, and economic dimensions of sustainability. Through a detailed case of ultrasound catheters, it demonstrates how remanufacturing can lead to resource conservation, cost savings, and enhanced product lifecycles in health care without compromising quality and patient safety. The study argues for systemic changes in healthcare practices to fully integrate remanufacturing, underscoring its role beyond a technical solution.