Even amidst a decline in religious affiliation, nearly half of the U.S. population still attends religious services at least once a month, and congregations remain the single largest non-profit organizational type across the nation. Therefore, congregational influence on political attitudes and behavior is a crucial line of inquiry. We analyze interviews of 94 congregational leaders to better understand why they address or avoid political issues when preaching. Our research reveals that clergy use theological and pragmatic reasoning to determine whether they explicitly include political discourse in their sermons. Our findings are noteworthy in that clergy from a wide range of religious traditions use similar reasoning, and the same rationale can lead different clergy to adopt contrasting approaches to political content in sermons. Thus, this paper provides nuanced insight into the relationship between religion and politics and may help foster greater mutual understanding in a deeply divided political and social climate.

OBJECTIVES/SPECIFIC AIMS: Rodent models can be used to study neonatal abstinence syndrome (NAS), but the applicability of findings from the models to NAS in humans is not well understood. The objective of this study was to develop a rat model of norbuprenorphine-induced NAS and validate its translational value by comparing blood concentrations in the norbuprenorphine-treated pregnant rat to those previously reported in pregnant women undergoing buprenorphine treatment. METHODS/STUDY POPULATION: Pregnant Long-Evans rats were implanted with 14-day osmotic minipumps containing vehicle, morphine (positive control), or norbuprenorphine (0.3–3 mg/kg/d) on gestation day 9. Within 12 hours of delivery, pups were tested for spontaneous or precipitated opioid withdrawal by injecting them with saline (10 mL/kg, i.p.) or naltrexone (1 or 10 mg/kg, i.p), respectively, and observing them for well-validated neonatal withdrawal signs. Blood was sampled via indwelling jugular catheters from a subset of norbuprenorphine-treated dams on gestation day 8, 10, 13, 17, and 20. Norbuprenorphine concentrations in whole blood samples were quantified using LC/MS/MS. RESULTS/ANTICIPATED RESULTS: Blood concentrations of norbuprenorphine in rats exposed to 1–3 mg/kg/d of norbuprenorphine were similar to levels previously reported in pregnant women undergoing buprenorphine treatment. Pups born to dams treated with these doses exhibited robust withdrawal signs. Blood concentrations of norbuprenorphine decreased across gestation, which is similar to previous reports in humans. DISCUSSION/SIGNIFICANCE OF IMPACT: These results suggest that dosing dams with 1–3 mg/kg/day norbuprenorphine produces maternal blood concentrations and withdrawal severity similar to those previously reported in humans. This provides evidence that, at these doses, this model is useful for testing hypotheses about norbuprenorphine that are applicable to NAS in humans.

Although borderline personality disorder (BPD) traits decline from adolescence to adulthood, comorbid psychopathology such as symptoms of major depressive disorder (MDD), alcohol use disorder (AUD), and drug use disorders (DUDs) likely disrupt this normative decline. Using a longitudinal sample of female twins (N = 1,763), we examined if levels of BPD traits were correlated with changes in MDD, AUD, and DUD symptoms from ages 14 to 24. A parallel process biometric latent growth model examined the contributions of genetic and environmental factors to the relationships between developmental components of these phenotypes. Higher BPD trait levels predicted a greater rate of increase in AUD and DUD symptoms, and higher AUD and DUD symptoms predicted a slower rate of decline of BPD traits from ages 14 to 24. Common genetic influences accounted for the associations between BPD traits and each disorder, as well as the interrelationships of AUD and DUD symptoms. Both genetic and nonshared environmental influences accounted for the correlated levels between BPD traits and MDD symptoms, but solely environmental influences accounted for the correlated changes between the two over time. Results indicate that higher levels of BPD traits may contribute to an earlier onset and faster escalation of AUD and DUD symptoms, and substance use problems slow the normative decline in BPD traits. Overall, our data suggests that primarily genetic influences contribute to the comorbidity between BPD features and substance use disorder symptoms. We discuss our data in the context of two major theories of developmental psychopathology and comorbidity.

A 2-yr experiment evaluated the effect of spring soil disturbance on the periodicity of weed emergence. At four locations across the northeastern United States, emerged weeds, by species, were monitored every 2 wk in both undisturbed plots and plots tilled in the spring with a rotary cultivator. Eight weed species including large crabgrass, giant and yellow foxtail, common lambsquarters, smooth pigweed, eastern black nightshade, common ragweed, and velvetleaf occurred at three or more site-years. Spring soil disturbance either had no effect or reduced total seedling emergence compared with undisturbed soils. Total seedling emergence for large crabgrass, giant foxtail, smooth pigweed, and common ragweed were on average, 1.4 to 2.6 times less with spring soil disturbance, whereas eastern black nightshade and velvetleaf were mostly unaffected by the soil disturbance. The influence of soil disturbance on yellow foxtail and common lambsquarters emergence varied between seasons and locations. Although the total number of emerged seedlings was often affected by the soil disturbance, with the exception of yellow foxtail and common ragweed, the periodicity of emergence was similar across disturbed and undisturbed treatments.

Evaluation of turfgrass performance at low nitrogen fertility levels is important because many home lawns are fertilized below common recommendations. The objective of this study was to evaluate visible quality and weed susceptibility of common and alternative cool season grasses under multiple management regimes in Wisconsin. A split-split plot completely randomized block design was used to evaluate ‘Kingfisher' Kentucky bluegrass (Kentucky bluegrass), ‘Kenblue' Kentucky bluegrass, ‘Victory II' chewings fescue, ‘Grande II' tall fescue, and ‘Jiffe II' perennial ryegrass. Each species was mowed at 3.5, 6.0, or 8.5 cm, and fertilized with 0, 98, or 196 kg ha−1 yr−1 of nitrogen. Visible quality and weed cover were evaluated four times annually for 3 yr. Tall fescue had the greatest turf quality across all treatments. Kingfisher Kentucky bluegrass, an improved variety, responded most dramatically to nitrogen fertilization, with quality rating improved from 5.1 to 7.1 when annual nitrogen applications totaled 196 kg ha−1 compared to the nonfertilized control. Kenblue Kentucky bluegrass, a common variety, had the greatest weed cover at all mowing heights and fertilizer rates. Assessment of common dandelion flowers by digital image analysis revealed that improved and common Kentucky bluegrass had greater common dandelion cover than fine or tall fescue when herbicides were withheld for 2.5 yr. Background soil fertility was found to have a significant impact on visible quality and weed cover. In an area with eroded, low-fertility soil, improved Kentucky bluegrass required 196 kg N ha−1 yr−1 to maintain high quality and limit weed invasion. These results suggest that tall fescue is best suited to low and high input conditions, while improved varieties of Kentucky bluegrass performed acceptably only under high inputs.

I examine a relatively underexplored aspect of Japan's early postwar history and seek to explain why attempts to establish a Japanese-style central intelligence agency (JCIA) in the 1950s were unsuccessful. I evaluate three competing explanations drawn from the level of international politics, focusing on US power resources and influence as well as liberal and constructivist styles of analysis—alliance politics, sectionalism, and the norm of antimilitarism—in order to shed light on the historical origins of Japan's intelligence apparatus, which is relatively underdeveloped and underfunded compared to other middle powers. It highlights the primacy of domestic factors over structural causes in explaining the decision not to establish a JCIA. In particular, I argue that the JCIA proposal failed primarily because of attacks on important proponents that, while sometimes driven by seemingly rational organizational interests, were nevertheless legitimated by growing antimilitaristic sentiments shared by elites from the political center to the left of the ideological spectrum. The newly emerging norm of antimilitarism was predicated largely on a fear of constraints on recently acquired civil and political liberties. These fears, manifested most prominently in vocal Diet and media opposition, were compounded by the norm of secrecy—an important element of intelligence activities—which served to heighten further speculation about the malign intent of postwar Japan's reconstituted intelligence system.