The identification of volatile pheromones attractive to and produced by many species within the family Cerambycidae (Coleoptera) has spurred development of synthetic pheromone lures that can be used to assess cerambycid populations and to monitor for invasive and rare species. We applied this method of trapping to examine cerambycid attraction to pheromone compounds and to initiate an analysis of the cerambycid communities within western Idaho, United States of America. A total of 8195 cerambycids, representing 67 species, 17 tribes, and 42 genera within six subfamilies of the Cerambycidae, were captured. Thirteen volatile pheromone lures were tested over three years, and a significant treatment effect was detected for nine cerambycid species. No significant differences were found among sites for species richness, diversity, or evenness. No significant differences were found among lures for species richness or diversity, but a significant difference was detected among lures for species evenness. We propose a method for designing a multicomponent lure, based on data from the target region, to maximise the number of species captured and to target specific cerambycid species within a targeted region.

This article emerged as the human species collectively have been experiencing the worst global pandemic in a century. With a long view of the ecological, economic, social, and political factors that promote the emergence and spread of infectious disease, archaeologists are well positioned to examine the antecedents of the present crisis. In this article, we bring together a variety of perspectives on the issues surrounding the emergence, spread, and effects of disease in both the Americas and Afro-Eurasian contexts. Recognizing that human populations most severely impacted by COVID-19 are typically descendants of marginalized groups, we investigate pre- and postcontact disease vectors among Indigenous and Black communities in North America, outlining the systemic impacts of diseases and the conditions that exacerbate their spread. We look at how material culture both reflects and changes as a result of social transformations brought about by disease, the insights that paleopathology provides about the ancient human condition, and the impacts of ancient globalization on the spread of disease worldwide. By understanding the differential effects of past epidemics on diverse communities and contributing to more equitable sociopolitical agendas, archaeology can play a key role in helping to pursue a more just future.

The COllaborative project of Development of Anthropometrical measures in Twins (CODATwins) project is a large international collaborative effort to analyze individual-level phenotype data from twins in multiple cohorts from different environments. The main objective is to study factors that modify genetic and environmental variation of height, body mass index (BMI, kg/m2) and size at birth, and additionally to address other research questions such as long-term consequences of birth size. The project started in 2013 and is open to all twin projects in the world having height and weight measures on twins with information on zygosity. Thus far, 54 twin projects from 24 countries have provided individual-level data. The CODATwins database includes 489,981 twin individuals (228,635 complete twin pairs). Since many twin cohorts have collected longitudinal data, there is a total of 1,049,785 height and weight observations. For many cohorts, we also have information on birth weight and length, own smoking behavior and own or parental education. We found that the heritability estimates of height and BMI systematically changed from infancy to old age. Remarkably, only minor differences in the heritability estimates were found across cultural–geographic regions, measurement time and birth cohort for height and BMI. In addition to genetic epidemiological studies, we looked at associations of height and BMI with education, birth weight and smoking status. Within-family analyses examined differences within same-sex and opposite-sex dizygotic twins in birth size and later development. The CODATwins project demonstrates the feasibility and value of international collaboration to address gene-by-exposure interactions that require large sample sizes and address the effects of different exposures across time, geographical regions and socioeconomic status.

The adoption of chemical fallow rotations in Pacific Northwest dryland winter wheat production has caused a weed species composition shift in which scouringrush has established in production fields. Thus, there has been interest in identifying herbicides that effectively control scouringrush in winter wheat–chemical fallow cropping systems. Field experiments were established in growers’ fields near Reardan, WA, in 2014, and The Dalles, OR, in 2015. Ten herbicide treatments were applied to mowed and nonmowed plots during chemical fallow rotations. Scouringrush stem densities were quantified the following spring and after wheat harvest at both locations. Chlorsulfuron plus MCPA-ester resulted in nearly 100% control of scouringrush through wheat harvest. Before herbicide application, mowing had no effect on herbicide efficacy. We conclude chlorsulfuron plus MCPA-ester is a commercially acceptable treatment for smooth and intermediate scouringrush control in winter wheat–chemical fallow cropping systems; however, the lack of a positive yield response when scouringrushes were controlled should factor into management decisions.

Platinum-group minerals (PGM) from placer deposits in Colombia, California, Oregon and Alaska were investigated with the electron microprobe, proton microprobe (μ-PIXE) and ion probe to analyse their major and trace element contents and 187Os/186Os isotopic ratios. Most of the grains in the samples investigated proved to be essentially homogeneous alloys of Pt-Fe and Os-Ir-Ru although a few of them contained inclusions of other PGM such as cooperite and laurite. Detailed analyses were undertaken on the Os-Ir-Ru alloy phases.

The 187Os/186Os isotope ratios fell into a range from 1.005 to 1.156 and are consistent with data published on PGM from other placer deposits from these regions. The ratios, together with the trace element data (and in particular the low rhenium content) determined by ion probe and μ-PIXE, indicate that crustal osmium was not incorporated in the grains and that no significant evolution of the 187Os/186Os ratios occurred during their history. These data, along with mineralogical and textural evidence, are consistent with a mantle origin for the grains through ultramafic intrusions, although the data do not entirely rule out alternative interpretations.

We present an indentation-scope that interfaces with confocal microscopy, enabling direct observation of the three-dimensional (3D) microstructural response of coatings on substrates. Using this method, we compared microns-thick polymer coatings on glass with and without silica nanoparticle filler. Bulk force data confirmed the >30% modulus difference, while microstructural data further revealed slip at the glass-coating interface. Filled coatings slipped more and about two times faster, as reflected in 3D displacement and von Mises strain fields. Overall, these data indicate that silica-doping of coatings can dramatically alter adhesion. Moreover, this method compliments existing theoretical and modeling approaches for studying indentation in layered systems.

Whether monozygotic (MZ) and dizygotic (DZ) twins differ from each other in a variety of phenotypes is important for genetic twin modeling and for inferences made from twin studies in general. We analyzed whether there were differences in individual, maternal and paternal education between MZ and DZ twins in a large pooled dataset. Information was gathered on individual education for 218,362 adult twins from 27 twin cohorts (53% females; 39% MZ twins), and on maternal and paternal education for 147,315 and 143,056 twins respectively, from 28 twin cohorts (52% females; 38% MZ twins). Together, we had information on individual or parental education from 42 twin cohorts representing 19 countries. The original education classifications were transformed to education years and analyzed using linear regression models. Overall, MZ males had 0.26 (95% CI [0.21, 0.31]) years and MZ females 0.17 (95% CI [0.12, 0.21]) years longer education than DZ twins. The zygosity difference became smaller in more recent birth cohorts for both males and females. Parental education was somewhat longer for fathers of DZ twins in cohorts born in 1990–1999 (0.16 years, 95% CI [0.08, 0.25]) and 2000 or later (0.11 years, 95% CI [0.00, 0.22]), compared with fathers of MZ twins. The results show that the years of both individual and parental education are largely similar in MZ and DZ twins. We suggest that the socio-economic differences between MZ and DZ twins are so small that inferences based upon genetic modeling of twin data are not affected.

Winter wheat grain contaminated with jointed goatgrass joints is often discounted as much as 20% by grain buyers. A mail survey to Nebraska farmers in 1984 identified jointed goatgrass as one of the ten worst weed problems in winter wheat, but a field survey to the same area in 1986 found it in less than 1% of surveyed fields. The objective of this survey was to map the geographic distribution and severity of jointed goatgrass contaminating winter wheat grain in western Nebraska. Jointed goatgrass was found in 25, 29, and 20% of all wheat samples collected in 1990, 1991, and 1992, respectively. Nebraska counties bordering Colorado were found to have the highest percentage of wheat samples contaminated with jointed goatgrass joints, ranging from 23% in Cheyenne county in 1992 to 61% in Keith county in 1991.

The tolerance of two proso millet cultivars to atrazine preemergence and postemergence applications of bromoxynil, clopyralid, dicamba, or metsulfuron plus amine formulations of 2,4-D was studied in the field. Proso millet grain yield, test weight, seed weight, moisture content, and plant height at harvest were not affected by any of the herbicide treatments, despite some early-season injury observed in 1990.

When glyphosate [N-(phosphonomethyl)glycine] was fed through the transpiration stream of excised bean (Phaseolus vulgaris L. ‘Red Kidney’) shoots, it was readily absorbed and transported to the leaves where it inhibited transpiration. Maximum inhibition (40 to 50%) resulted from tissue concentrations of 50 to 3000 nmoles glyphosate/g fresh weight, while transpiration was significantly inhibited (20%) by as little as 15 nmoles/g fresh weight. A combination of 5 X 10-4M tyrosine (Tyr) and 5 x 10-4M phenylalanine (Phe) supplied continuously through the transpiration stream prevented the inhibition of transpiration by glyphosate during the course of 8-h experiments. Tyrosine (10-3M) alone delayed the transpiration response to glyphosate by 2 to 3 h but 10-3M Phe alone did not affect the inhibition by glyphosate. Levels of endogenous Tyr and Phe were about 50% lower in glyphosate-treated leaves than in the controls 6 h after treatment.

Crop yield loss–weed density relationships critically influence calculation of economic thresholds and the resulting management recommendations made by a bioeconomic model. To examine site-to-site and year-to-year variation in winter Triticum aestivum L. (winter wheat)–Aegilops cylindrica Host. (jointed goatgrass) interference relationships, the rectangular hyperbolic yield loss function was fit to data sets from multiyear field experiments conducted at Colorado, Idaho, Kansas, Montana, Nebraska, Utah, Washington, and Wyoming. The model was fit to three measures of A. cylindrica density: fall seedling, spring seedling, and reproductive tiller densities. Two parameters: i, the slope of the yield loss curve as A. cylindrica density approaches zero, and a, the maximum percentage yield loss as A. cylindrica density becomes very large, were estimated for each data set using nonlinear regression. Fit of the model to the data was better using spring seedling densities than fall seedling densities, but it was similar for spring seedling and reproductive tiller densities based on the residual mean square (RMS) values. Yield loss functions were less variable among years within a site than among sites for all measures of weed density. For the one site where year-to-year variation was observed (Archer, WY), parameter a varied significantly among years, but parameter i did not. Yield loss functions differed significantly among sites for 7 of 10 comparisons. Site-to-site statistical differences were generally due to variation in estimates of parameter i. Site-to-site and year-to-year variation in winter T. aestivum–A. cylindrica yield loss parameter estimates indicated that management recommendations made by a bioeconomic model cannot be based on a single yield loss function with the same parameter values for the winter T. aestivum-producing region. The predictive ability of a bioeconomic model is likely to be improved when yield loss functions incorporating time of emergence and crop density are built into the model's structure.

Field studies were conducted from 1990 through 1997 to evaluate the long-term effect of 2- and 3-yr rotations on the control of downy brome, jointed goatgrass, and feral rye in winter wheat. At the completion of the study, jointed goatgrass and feral rye densities averaged 8 plants/m2 and < 0.1 plant/m2 for the 2- and 3-yr rotations, respectively. Downy brome densities averaged < 0.5 plant/m2 for both the 2- and 3-yr rotations, with no treatment differences observed. Winter annual grasses were not eradicated after two cycles of the 3-yr rotations, but weed densities were reduced 10-fold compared to densities after one cycle and more than 100-fold compared with the 2-yr rotations. Wheat grain contamination with dockage and foreign material followed a similar trend. The 3-yr rotations were economically competitive with 2-yr rotations and provided superior control of the winter annual grass weeds.

Proso millet is a short-season summer annual grass that is well adapted to the central Great Plains. Proso millet is commonly planted as a summer crop when winter wheat stands are lost due to adverse conditions. Sulfonylurea herbicides labeled for use in winter wheat prohibit planting proso millet for intervals up to 10 mo following application. A series of greenhouse and field studies determined proso millet tolerance to CGA-152005, metsulfuron, and triasulfuron soil residue. In the greenhouse, proso millet was not affected by soil-applied CGA-152005 at doses up to 160 g ai/ha, while metsulfuron and triasulfuron doses of 4 and 15 g ai/ha, respectively, inhibited proso millet biomass accumulation. In the field, metsulfuron and triasulfuron caused early season stunting and chlorosis at doses two to four times those recommended; however, grain yields were not affected. Organic matter and clay content were highly correlated with proso millet growth response to the herbicides under greenhouse conditions, but in the field, soil pH may have influenced herbicide bioavailability.

Jointed goatgrass is a problem weed in winter wheat production areas of the Great Plains. Winter wheat seeding rates are easily adjusted by the growers and influence competition by some weeds. Field experiments were initiated in Kansas, Nebraska, and Wyoming using winter wheat cultivars selected from leading adapted cultivars from each region to determine the effect of wheat plant density in the fall on jointed goatgrass competitiveness. Three winter wheat seeding rates (50, 67, and 84 kg seeds/ha) were used at Hays, KS, and Sidney, NE, and four seeding rates (33, 50, 67, and 101 kg seeds/ha) were used at Torrington and Archer, WY. An analysis of covariance model was fit with winter wheat fall plant density as the covariate. In 1996, winter wheat grain contamination (dockage) was reduced at the rate of about 6% for every 10 additional wheat plants/m2 above the threshold density of 70 plants/m2 at Archer, WY, and at the rate of about 0.5% for every 10 additional wheat plants/m2 above the threshold density of 110 plants/m2 at Hays, KS. At Hays the reduction occurred only with the semidwarf cultivar ‘Vista’. Increased wheat density reduced jointed goatgrass reproductive tillers in four out of six location–year combinations and biomass in two out of four location–year combinations. Despite the lack of a consistent reduction in jointed goatgrass competitiveness as the result of increased wheat density, increased seeding rates may be a good, low-cost, long-term investment as part of an integrated jointed goatgrass control program in winter wheat.

Secale cereale is a serious weed problem in winter Triticum aestivum–producing regions. The interference relationships and economic thresholds of S. cereale in winter T. aestivum in Colorado, Kansas, Nebraska, and Wyoming were determined over 4 yr. Winter T. aestivum density was held constant at recommended planting densities for each site. Target S. cereale densities were 0, 5, 10, 25, 50, or 100 plants m−2. Secale cereale–winter T. aestivum interference relationships across locations and years were determined using a negative hyperbolic yield loss function. Two parameters—I, which represents the percent yield loss as S. cereale density approaches zero, and A, the maximum percent yield loss as S. cereale density increases—were estimated for each data set using nonlinear regression. Parameter I was more stable among years within locations than among locations within years, whereas maximum percentage yield loss was more stable across locations and years. Environmental conditions appeared to have a role in the stability of these relationships. Parameter estimates for I and A were incorporated into a second model to determine economic thresholds. On average, threshold values were between 4 and 5 S. cereale plants m−2; however, the large variation in these threshold values signifies considerable risk in making economic weed management decisions based upon these values.

Field, greenhouse, and laboratory studies were conducted to determine the effect of MCPA ester, fertilizer type, and fertilizer rate on feral rye control with imazamox. In field studies near Sidney, NE, increasing the concentration of liquid ammonium phosphate (10–34–0) from 2.5 to 50% of the spray solution decreased feral rye control with imazamox by as much as 73%. Conversely, adding MCPA ester to imazamox significantly increased feral rye control in field studies by up to 77%. Initial greenhouse studies confirmed the liquid ammonium phosphate antagonism effect, but subsequent greenhouse studies were inconsistent with regard to the interaction between fertilizer and imazamox. At least one source of liquid ammonium phosphate was shown not to be antagonistic, and therefore fertilizer source or contaminants may be responsible for initial field observations. Greenhouse studies confirmed the synergistic interaction between MCPA and imazamox. MCPA ester applied at 560 g ai ha−1 decreased the rate of imazamox required to cause 50% reduction in feral rye dry weight (GR50) to 13 g ha−1 compared to 35 g ha−1 for imazamox alone. Although addition of MCPA ester increased 14C-imazamox absorption by 8% in laboratory studies, less 14C translocated out of the treated leaf; therefore the mechanism of synergism does not appear to be related to imazamox absorption or translocation.

Three models that empirically predict crop yield from crop and weed density were evaluated for their fit to 30 data sets from multistate, multiyear winter wheat–jointed goatgrass interference experiments. The purpose of the evaluation was to identify which model would generally perform best for the prediction of yield (damage function) in a bioeconomic model and which model would best fulfill criteria for hypothesis testing with limited amounts of data. Seven criteria were used to assess the fit of the models to the data. Overall, Model 2 provided the best statistical description of the data. Model 2 regressions were most often statistically significant, as indicated by approximate F tests, explained the largest proportion of total variation about the mean, gave the smallest residual sum of squares, and returned residuals with random distribution more often than Models 1 and 3. Model 2 performed less well based on the remaining criteria. Model 3 outperformed Models 1 and 2 in the number of parameters estimated that were statistically significant. Model 1 outperformed Models 2 and 3 in the proportion of regressions that converged on a solution and more readily exhibited an asymptotic relationship between winter wheat yield and both winter wheat and jointed goatgrass density under the constraint of limited data. In contrast, Model 2 exhibited a relatively linear relationship between yield and crop density and little effect of increasing jointed goatgrass density on yield, thus overpredicting yield at high weed densities when data were scarce. Model 2 had statistical properties that made it superior for hypothesis testing; however, Model 1's properties were determined superior for the damage function in the winter wheat–jointed goatgrass bioeconomic model because it was less likely to cause bias in yield predictions based on data sets of minimum size.