Changes in abundance and distribution of marine top predators can indicate environmental change or anthropogenic pressure requiring management response. Here, we used an extensive dataset (21 years) to conduct a spatial and temporal analysis of grey seal strandings in Cornwall and the Isles of Scilly, close to the southern edge of the breeding range of the species. A total of 2007 strandings were reported from 2000 to 2020, increasing by 474% from 35 to 201 individuals per year during this period. The continued rise in strandings was consistent across all life stages and timeframes (5, 10 and 20 years), underpinning the suggestion of increasing abundance in the region. The observed seasonality differed by life stage, coinciding with the increased presence of animals near the coast for key life phases such as breeding, moulting and pupping. Strandings are widely distributed across the coast of Cornwall and the Isles of Scilly; however, most strandings were recorded on the north coast of Cornwall (70%) where major pupping and haul out sites are found. Despite hosting several pupping and haul out sites, a small proportion was recorded on the Isles of Scilly (5%) where it is thought that strandings are particularly underreported. Describing baselines in magnitude of strandings and life-stage compositions across space and time allows future deviations in frequency, demographic composition or spatial distribution to be detected and investigated. We demonstrate the utility of long-term citizen science data to provide valuable and cost-effective information on the distribution and abundance of a highly mobile marine mammal.

Concerns over elephant welfare in UK zoos have implications for their future in captivity. To monitor improvements made to elephant welfare in UK zoos, non-invasive, valid and reliable indicators of welfare are needed. Using a rapid review strategy and critical appraisal tool, we aimed to appraise evidence from peer-reviewed literature on potential welfare indicators for captive elephants. Scopus, Web of Knowledge and Ovid were searched in January 2014 using terms relevant to captive elephants and welfare assessment. Inclusion and exclusion criteria were applied and remaining articles were critically appraised against a specially designed welfare indicator appraisal tool. Thirty-seven unique indicators of welfare were extracted from 30 peer-reviewed papers which met the inclusion criteria. Behavioural measures of welfare (n = 21) were more common than either physical (n = 11) or physiological (n = 5) measures. Stereotypies were the most frequently used behavioural measure, glucocorticoids were the most frequently used physiological measure and body condition scores were the most frequently used physical measure. There was most support for the following indicators of improved welfare state: reduced stereotypies, reduced glucocorticoids and improved body condition scores. Additional measures which require further validation but had strong associations with the most supported measures, and thus have potential use in welfare assessment, were: increased lying rest and positive social interactions. Further validation of the described measures is needed, but this information forms a crucial part of the knowledge required to efficiently monitor and improve the welfare of elephants in captivity.

Recent concerns over the welfare of elephants in UK zoos have implications for their future in captivity, and it is clear that improvements in welfare should be made. Evidence suggests that the knowledge of experienced stakeholders is vital to captive animal welfare assessment. However, there have been few attempts to consult with zoo personnel and other stakeholders on the assessment of elephant welfare, and much of their valuable knowledge of routine husbandry has not been captured in the published literature. As part of a research project commissioned by the Department for Environment Food and Rural Affairs, open response focus groups and workshop discussions were conducted with representatives from 15 UK elephant-holding facilities, and other experts in the welfare and behaviour of captive or free-ranging elephants. Participants described three broad categories of welfare indicators: behavioural, physical and physiological. Resources perceived to be of importance to elephants included aspects of the physical environment, such as feeding opportunities and appropriate substrate, and aspects of the social environment, including group size and relatedness. The data obtained during this study can be used to develop an elephant welfare assessment strategy, informed by the knowledge and expertise of experienced stakeholders, and for consideration of potential changes to guidelines for managing elephants in captivity. Our approach to capturing the views of those who work closely with captive species could be applied elsewhere, in order to draw upon the extensive knowledge of expert stakeholders and consider ways to improve the welfare of captive animals.

This study quantified CO2 emissions from tropical peat swamp soils in Brunei Darussalam. At each site, soil was collected from areas of intact and degraded peat and CO2 flux, and total organic content were measured ex situ. Soil organic content (~20–99%) was not significantly different between intact and degraded forest samples. CO2 flux was higher for intact forest samples than degraded forest samples (~1.0 vs. ~0.6 μmol CO2 m−2 s−1, respectively) but did not differ among forest locations. From our laboratory experiments, we estimated a potential emissions of ~10–20 t CO2 ha−1 y−1 which is in the lower range of values reported for other tropical peat swamps. However, our results are likely affected by unmeasured variation in root respiration and the lability of resident carbon. Overall, these findings provide experimental evidence to support that clearance of tropical peat swamp forests can increase CO2 emissions due to faster rates of decomposition.

Toxoplasma gondii, a zoonotic parasite of global importance, infects all endothermic vertebrates, with extensive health implications. The prevalence of this parasite is seldom monitored in wildlife. Here, a semi-aquatic species, the Eurasian otter (Lutra lutra) was used as a model to assess the potential effect of climate, land cover and biotic factors on T. gondii seroprevalence in British wildlife. The Sabin–Feldman cytoplasm-modifying dye test identified T. gondii antibodies in 25·5% of blood samples from otters found dead, mainly as road kill, in England and Wales, between 2004 and 2010. Otters in the east of England were more likely to be infected with T. gondii than those in western regions. Land cover and temperature are key determinants of T. gondii infection risk, with more infection in arable areas and lower infection where temperatures are higher. The probability of T. gondii infection increased with host age, reflecting cumulative exposure with time, but there was no association between T. gondii seroprevalence and cause of host death.

The radiocarbon content and stable isotope composition of soil carbonate are best described by a dynamic system in which isotopic reequilibration occurs as a result of recurrent dissolution and reprecipitation. Depth of water penetration into the soil profile, as well as soil age, determines the degree of carbonate isotope reequilibration. We measured δ13C, δ18O and radiocarbon content of gravel rinds and fine (<2 mm) carbonate in soils of 3 .different ages (1000, 3800, and 6300 14 C yr B.P.) to assess the degree to which they record and preserve a climatic signal. In soils developing in deposits independently dated at 3800 and 6300 radiocarbon yr B.P., carbonate radiocarbon content above 40 cm depth suggests continual dissolution and reprecipitation, presumably due to frequent wetting events. Between 40 and 90 cm depth, fine carbonate is dissolved and precipitated as rinds that are not redissolved subsequently. Below 90 cm depth in these soils, radiocarbon content indicates that inherited, fine carbonate undergoes little dissolution and reprecipitation. In the 3800- and 6300-yr-old soils, δ13C in rind and fine carbonate follows a decreasing trend with depth, apparently in equilibrium with modern soil gas, as predicted by a diffusive model for soil CO2. δ18O also decreases with depth due to greater evaporative enrichment above 50 cm depth. In contrast, carbonate isotopes in a 1000-yr-old deposit do not reflect modern conditions even in surficial horizons; this soil has not undergone significant pedogenesis. There appears to be a lag of at least 1000 but less than 3800 yr before carbonate inherited with parent material is modified by ambient climatic conditions. Although small amounts of carbonate are inherited with the parent material, the rate of pedogenic carbonate accumulation indicates that Ca is derived primarily from eolian and rainfall sources. A model describing carbonate input and radiocarbon decay suggests that fine carbonate below 90 cm is mostly detrital (inherited) and that carbonate rinds have been forming pedogenically at a constant rate since alluvial fans were deposited.

The relationship between the stable C-isotope composition of the soil environment and modern climate and vegetation was determined empirically along a present-day climatic transect in the eastern Mojave Desert. The δ13C of the soil CO2 and carbonates decreased with increasing elevation and plant density, even though plant assemblages at all elevations were isotopically similar. Several factors, including differences in the ratios of pedogenic of limestone calcite and differences in past vegetation, were considered as explanations of this trend, However, it appears that in the sparsely vegetated Mojave Desert, the δ13C of pedogenic carbonate is controlled by differences in plant density and biological activity. This relationship may provide a tool for assessing past vegetational densities, as long as the vegetation is isotopically homogeneous.

Increasing recognition of the extent to which nitrous oxide (N2O) contributes to climate change has resulted in greater demand to improve quantification of N2O emissions, identify emission sources and suggest mitigation options. Agriculture is by far the largest source and grasslands, occupying c. 0·22 of European agricultural land, are a major land-use within this sector. The application of mineral fertilizers to optimize pasture yields is a major source of N2O and with increasing pressure to increase agricultural productivity, options to quantify and reduce emissions whilst maintaining sufficient grassland for a given intensity of production are required. Identification of the source and extent of emissions will help to improve reporting in national inventories, with the most common approach using the IPCC emission factor (EF) default, where 0·01 of added nitrogen fertilizer is assumed to be emitted directly as N2O. The current experiment aimed to establish the suitability of applying this EF to fertilized Scottish grasslands and to identify variation in the EF depending on the application rate of ammonium nitrate (AN). Mitigation options to reduce N2O emissions were also investigated, including the use of urea fertilizer in place of AN, addition of a nitrification inhibitor dicyandiamide (DCD) and application of AN in smaller, more frequent doses. Nitrous oxide emissions were measured from a cut grassland in south-west Scotland from March 2011 to March 2012. Grass yield was also measured to establish the impact of mitigation options on grass production, along with soil and environmental variables to improve understanding of the controls on N2O emissions. A monotonic increase in annual cumulative N2O emissions was observed with increasing AN application rate. Emission factors ranging from 1·06–1·34% were measured for AN application rates between 80 and 320 kg N/ha, with a mean of 1·19%. A lack of any significant difference between these EFs indicates that use of a uniform EF is suitable over these application rates. The mean EF of 1·19% exceeds the IPCC default 1%, suggesting that use of the default value may underestimate emissions of AN-fertilizer-induced N2O loss from Scottish grasslands. The increase in emissions beyond an application rate of 320 kg N/ha produced an EF of 1·74%, significantly different to that from lower application rates and much greater than the 1% default. An EF of 0·89% for urea fertilizer and 0·59% for urea with DCD suggests that N2O quantification using the IPCC default EF will overestimate emissions for grasslands where these fertilizers are applied. Large rainfall shortly after fertilizer application appears to be the main trigger for N2O emissions, thus applicability of the 1% EF could vary and depend on the weather conditions at the time of fertilizer application.

Many reef-building corals photoacclimate by increasing levels of chlorophyll per microalgal cell with depth, but mechanisms of photoacclimation in coral reef sea anemones remain poorly understood. We determined variation in ambient irradiance and patterns of abundance, microhabitat use, chlorophyll and microalgal cell concentrations in clownfish sea anemones Entacmaea quadricolor from 0 to 43 m depth on a coral reef at Aqaba, Jordan, northern Red Sea. In shallow areas, anemones occupied shaded reef microhabitats exposed to significantly lower irradiance than in the adjacent open water, but on the deep reef slope they occupied unshaded habitats. Anemone abundances were the highest observed thus far in the Red Sea, and peaked at mid depth on the reef slope. Microalgal abundance in anemone tentacles increased four-fold from the shallow to deep reef, while chlorophyll-a concentrations per algal cell did not vary significantly with depth. We conclude that E. quadricolor photoacclimates using two major mechanisms: (1) occurrence in shaded microhabitats when shallow, thus reducing exposure to high irradiance, and (2) increasing microalgal abundance with depth, thereby enhancing photosynthetic efficiency at low irradiance.

About a year ago Mr. F. M. Brown called the writer's attention to the aberrant venation of one secondary of a specimen of Erebia magdalena Strecker. The aberration, of the type shown in Figure I, consists in the presence of an extra cross-vein connecting veins Rs and M1 a few millimeters beyond the end of the cell. Later, in studying series of other species of the genus, a similar variation from the normal (Figure 2) turned up in several specimens. The facts are presented in the following table, in which the results of examination of some material from the genus Oeneis is also included.

A series of 15 embryonic stages of Periplaneta americana Linnaeus is defined on the basis of external morphological characters. These stages permit the study of internal development of embryos whose chronological age is unknown or from embryos incubated at different or varying temperatures. The stages, while extremely useful, are entirely arbitrary divisions of a continuous developmental process and a larger or smaller number of them could have been designated.

The cervical and thoracic musculature of the first-stage nymph is described for the first time, and its genesis is followed in the embryo. The embryonic and nymphal muscles are compared with those that have been reported for the adult.

In the course of a series of dissections of various species of Pieridae, the author noticed what appeared tobe a costal vein in the forewing of a specimen of Archonias bellona Cramer. Further examination showed that there was indeed a costal vein present here, and this prompted a scrutiny of a considerable body of material in this ganus and others allied to it, the results of which are given in tabular form below.

Woodchip pads can be a sustainable alternative to the overwintering of stock on grassland or in conventional housing and can offer benefits in improved animal performance, improved health and environmentally sustainable options for the management of the effluent resulting from the animal excreta (dung, urine and rainfall over the pad). Detailed observations were made on effluent flow and quality from woodchip pads on two commercial farms in the UK, one in Powys (Wales) and the other in Leicestershire (England), over a period of 8 months in 2009/10. Flow data and hydrological characteristics, reported in the companion to the current paper (Jackson & Smith 2012), were combined with the results of effluent sample and soiled woodchip analyses, together with records of animal numbers and activity on the pads, to calculate nutrient fluxes and nutrient balances across the pad for defined periods. Nutrient balances showed that, of the estimated nutrient inputs in animal excreta deposited on the pad, only 0·05–0·10 of the N and P were contained in the effluent draining from the pad, with the rest (>0·90 of N and P inputs) retained in the solids accumulating in the surface layers of soiled woodchip, ‘spent timber residues’ (STR). The STR was similar in analysis to straw-based farmyard manure (FYM), high in organic N, and land spreading of this material should be managed in a similar way to FYM. It also appears suitable for application to grassland, except when based on coarse woodchips. These results confirm the hypothesis that the effluent draining from the pads should be considered as consistently similar to dirty water rather than slurry, as in the current rules associated with Nitrate Vulnerable Zones (NVZs) in England and Wales.

Little is known about the cleaning behaviour of shrimps in comparison to that of cleaner fish, and only recently have cleaner shrimps been shown to remove parasites effectively from coral reef fish. Here we describe patterns of cleaning interactions between Pederson shrimp Ancylomenes pedersoni and fish clients in St Thomas, US Virgin Islands. Clients observed here were members of at least 16 fish families, including three previously unreported client families. Most cleans lasted <20 seconds; surgeonfish were cleaned most frequently, but lizardfish and groupers received the longest cleans. The shrimp formed social groups of varying sizes on individuals of the host sea anemone Bartholomea annulata, which served as the centres of their cleaning stations. The number and duration of cleans per station increased with the number of resident shrimp, however most anemones hosted small groups of fewer than four individuals, while larger groups of up to nine individuals were relatively rare. Some client fish chased away other fish and competitively excluded them from anemone stations. We conclude that these shrimp clean a wide diversity of clients, vary their clean duration with fish identity, and clean more when in large groups. In addition, clients in part control these patterns of interaction by interfering with access to these stations by other clients.

Consider uniform flow past four slender bodies with elliptical cross-section of constant ellipticity along the length of 0, 0.125, 0.25 and 0.375, respectively, for each body. Here, ellipticity is defined as the ratio of the semiminor axis of the ellipse to the semimajor axis. The bodies have a pointed nose which gradually increases in cross-section with a radius of curvature 419 mm to a mid-section which then remains constant up to a blunt end section with semimajor axis diameter 160 mm, the total length of all bodies being 800 mm. The bodies are side-mounted within a low-speed wind tunnel with an operational wind speed of the order 30 m s−1. The side force (or lift) is measured within an angle of attack range of −3° to 3° such that the body is rotated about the major axis of the ellipse cross-section. The lift slope is determined for each body, and how it varies with ellipticity. It is found that this variance follows a straight line which steadily increases with increasing ellipticity. It is shown that this result is predicted by a recently developed Oseen flow slender body theory, and cannot be predicted by either inviscid flow slender body theory or viscous crossflow theories based upon the Allen and Perkins method.

If ewes consumed a high-salt diet or saltbush during the last 3 months of pregnancy and for 3 weeks after birth, we expected the renin activity of their lamb to be suppressed at birth and at 3 weeks of age. We also expected an increase in the concentration of cations other than sodium in the ewe’s milk and an increase in the plasma Na concentration of the lamb at birth. To test these hypotheses, Merino ewes were fed a high-salt diet (14% NaCl) in an animal house and compared to control ewes eating a control diet (2% NaCl). In addition, we compared ewes grazing saltbush (about 13% salt in diet) to ewes grazing pasture from day 60 of pregnancy to 3 weeks after birth. Lambs born to ewes consuming saltbush had 85% lower (P < 0.001) renin activity than offspring from ewes consuming pasture at 3 weeks of age. Similarly, lambs born to ewes consuming a high-salt diet had 20% lower renin activity at birth and 3 weeks (P = 0.07). Feeding ewes a high-salt diet or saltbush altered the mineral composition of the milk; the largest change was a 10% increase in K levels (P ⩽ 0.05). Consuming a high-salt diet or saltbush lowered the plasma Na of ewes at 130 days of gestation (by 3–5 mmol/l; P < 0.001), but only lambs from ewes fed the high-salt diet had a lower plasma Na at birth (P < 0.05). Suppression of the renin activity of lambs could lead to permanent physiological changes in salt balance in later life.