The perinatal period has gained increasing attention from developmental psychopathologists; however, experiences during birth have been minimally examined using this framework. The current study aimed to evaluate longitudinal associations between childhood maltreatment, negative birth experiences, and postpartum mental health across levels of self-reported emotion dysregulation and respiratory sinus arrhythmia (RSA). Expectant mothers (N = 223) participated in a longitudinal study from the third trimester of pregnancy to 7 months postpartum. Participants contributed prenatal resting RSA and completed questionnaires prenatally, 24 hours after birth, and 7 months postpartum. Results indicated that more childhood maltreatment was associated with higher birth fear and postpartum anxiety and depressive symptoms. Resting RSA moderated the association between childhood maltreatment and birth fear, such that more childhood maltreatment and higher resting RSA were associated with increased birth fear. Additionally, self-reported prenatal emotion dysregulation moderated the association between childhood maltreatment and postpartum depressive symptoms, such that more childhood maltreatment and higher emotion dysregulation were associated with increased depressive symptoms. Emotion dysregulation across multiple levels may amplify vulnerability to negative birth experiences and postpartum psychopathology among individuals with childhood maltreatment histories. Thus, emotion dysregulation in the context of trauma-informed care may be worthwhile intervention targets during the perinatal period.

To combat the decline in North American grasslands and prairies, innovative strategies to establish new native grass and forb plantings must be considered. Integrated vegetation management entails the use of many practices to cultivate desirable vegetation along roadsides, including mowing, applying herbicides, burning, and replanting. Currently, only a limited selection of postemergence herbicides are available to improve native plant establishment along roadsides. A greenhouse herbicide screen that included four postemergence herbicides registered for use on Conservation Reserve Program (CRP) acres and rights-of-way was conducted to test their safety for use on four native grasses (big bluestem, buffalograss, sideoats grama, and switchgrass) and seven forb species (ashy sunflower, black-eyed Susan, butterfly milkweed, desert false indigo, Illinois bundleflower, Mexican hat plant, and purple coneflower). Clopyralid (689 g ae ha−1), metsulfuron (4.18 g ai ha−1), and quinclorac (418 g ai ha−1) applied at labeled rates caused no injury to the native grass species or butterfly milkweed. However, florpyrauxifen-benzyl (38.4 g ai ha−1) caused significant injury to buffalograss and switchgrass. None of the herbicides tested were universally safe to use on all forb species evaluated in this trial, with each herbicide causing unacceptable injury (≥25%) to one or more forb species. None of the herbicides studied here would be completely safe for use on mixed stands of native grasses and native forbs at the seedling growth stage, indicating that prairie establishment must use alternative chemistries, plant mixes with fewer species, or avoid postemergence applications shortly after emergence of native forbs.

Around 1000 years ago, Madagascar experienced the collapse of populations of large vertebrates that ultimately resulted in many species going extinct. The factors that led to this collapse appear to have differed regionally, but in some ways, key processes were similar across the island. This review evaluates four hypotheses that have been proposed to explain the loss of large vertebrates on Madagascar: Overkill, aridification, synergy, and subsistence shift. We explore regional differences in the paths to extinction and the significance of a prolonged extinction window across the island. The data suggest that people who arrived early and depended on hunting, fishing, and foraging had little effect on Madagascar’s large endemic vertebrates. Megafaunal decline was triggered initially by aridification in the driest bioclimatic zone, and by the arrival of farmers and herders in the wetter bioclimatic zones. Ultimately, it was the expansion of agropastoralism across both wet and dry regions that drove large endemic vertebrates to extinction everywhere.

The Society for Healthcare Epidemiology of America, the Association of Professionals in Infection Control and Epidemiology, the Infectious Diseases Society of America, and the Pediatric Infectious Diseases Society represent the core expertise regarding healthcare infection prevention and infectious diseases and have written multisociety statement for healthcare facility leaders, regulatory agencies, payors, and patients to strengthen requirements and expectations around facility infection prevention and control (IPC) programs. Based on a systematic literature search and formal consensus process, the authors advocate raising the expectations for facility IPC programs, moving to effective programs that are:

• Foundational and influential parts of the facility’s operational structure

• Resourced with the correct expertise and leadership

• Prioritized to address all potential infectious harms

This document discusses the IPC program’s leadership—a dyad model that includes both physician and infection preventionist leaders—its reporting structure, expertise, and competencies of its members, and the roles and accountability of partnering groups within the healthcare facility. The document outlines a process for identifying minimum IPC program medical director support. It applies to all types of healthcare settings except post-acute long-term care and focuses on resources for the IPC program. Long-term acute care hospital (LTACH) staffing and antimicrobial stewardship programs will be discussed in subsequent documents.

Objectives/Goals: The aim of the study is to identify resistance factors for substance use (i.e., factors that explicitly help to avoid or reduce drug use). Identification of resistance factors could inform strategies that seek to reduce the prevalence of substance use and related disorders. Methods/Study Population: Adult twins aged 30–70 years were recruited from the Mid-Atlantic Twin Registry. A mixed-method approach, group concept mapping, was used to identify factors influencing participants to resist using substances. Approximately 155 participants produced 97 statements reflecting substance use resistance factors. Hierarchical cluster analysis and multidimensional scaling assessed how participants sorted and rated statements for their lifetime and current importance. Factor analysis was used to reduce data dimensionality. Reliability analyses were conducted to identify a subset of statements anticipated to consistently represent each cluster. Results were shared with participants to assess accuracy with their experiences. Results/Anticipated Results: Participants sorted 97 statements into 9 thematic clusters: (1) Controlling Personal, Negative Consequences; (2) Concern About Health and Well-being; (3) Lack of Desire; (4) Outside Influences; (5) Social Norms and My Reputation; (6) Career and Legal Impacts, (7) Avoiding Harm to Family and Relationships; (8) Preserving Family Relationships; and (9) Family and Friends Impact on Me. Participants consistently identified health concerns as an important substance use resistance factor. The statements will be further reduced to represent a smaller subset for future use as a scale to measure exposure to resistance factors. Discussion/Significance of Impact: Health concerns related to substance use were identified as an important resistance factor. This has been supported by research on smoking cessation and implemented in smoking prevention campaigns. Therefore, prioritizing health-related outcomes in prevention may be important to reduce substance use prevalence.

Prior to the Civil War, the US and state governments required the modern licensing of only three occupations, doctors, lawyers, and ship pilots. Most other references to licensing in the 15,000 surveyed antebellum statutes referred to licensing in general terms. Those that referred to the “licensing” of occupations clearly referenced a type of tax or regulation of occupations thought sinful or diplomatically sensitive, like Indian trading and privateering. In other words, the presumption of occupational freedom that developed in medieval and early modern Britain transferred to the colonies and the United States. Only with the rise of Progressivism did modern occupational licensing become common, thus adding weight to economic critiques of the current system.

The articles compiled here offer examples of how the impacts of anthropogenic climate change in coastal settings are monitored and measured, how the broader public can be involved in these efforts, and how planning for mitigation can come about. The case studies are drawn from the southeastern United States and the British Isles, and they indicate the great potential that cooperating communities of practice can offer for addressing climate-change impacts on cultural heritage.

The impact of required durations of therapy for antibiotic orders at the time of order entry has not been reported. Requiring ordering clinicians to enter stop dates at the time of antibiotic order entry decreased DOT/1000 patient days for orders with empiric indication from 154 to 119 (–34.9 (–55.7 to –14)).

From the original Stanford-Binet scale, those items passed by between 10 and 90 per cent of a group of ten-year-old children were analyzed by the centroid method. Upon rotation, there appeared a common factor, for which two explanatory hypotheses are offered, the more tenable being that it is an effect of maturation. Primary factors tentatively identified are Number, Space, Imagery, Verbal Relations and Induction. A sixth factor apparently involves a reasoning ability and a seventh can not be interpreted.

The Colorado Immersion Training in Community Engagement (CIT) program supports a change in the research trajectory of junior faculty, early career researchers, and doctoral students toward Community-Based Participatory Research (CBPR). CIT is within the Community Engagement and Health Equity Core (CEHE) at the Colorado Clinical and Translational Sciences Institute (CCTSI), an NIH-funded Clinical and Translational Science award. This Translational Science Case Study reports on CIT’s impacts from 2010 to 2019. A team from The Evaluation Center at the University of Colorado Denver utilized four primary data sources: administrative records, participant written reflections, participant and Community Research Liaison (CRL) interviews, and community partner surveys. Data were analyzed using the framework of CBPR principles and the conceptual logic model. CIT trained 122 researchers in CBPR through embedded education within various Colorado communities. CIT Alumni secured ∼$8,723,000 in funding between CCTSI Pilot Grants and external funding. Also, CIT alumni implemented CBPR into curricula and community programming and developed deep, lasting relationships. Further key learnings include the crucial role of CRLs in building relationships between university and community partners and how CIT may serve as a mechanism to improve historical mistrust between communities and universities.

In Georgia plasticulture vegetable production, a single installation of plastic mulch is used for up to five cropping cycles over an 18-mo period. Preplant applications of glyphosate and glufosinate ensure fields are weed-free before transplanting, but recent data suggest that residual activity of these herbicides may pose a risk to transplanted vegetables. Glyphosate and glufosinate were applied preplant in combination with three different planting configurations, including 1) a new plant hole into new mulch, 2) a preexisting plant hole, 3) or a new plant hole spaced 15 cm from a preexisting plant hole (adjacent). Following herbicide application, overhead irrigation was used to remove residues from the mulch before punching transplanting holes for tomato, cucumber, or squash. Visible injury; widths; biomass; and yield of tomato, cucumber, or squash were not influenced by herbicide in the new mulch or adjacent planting configurations. When glyphosate was applied at 5.0 kg ae ha−1 and the new crop was planted into preexisting holes, tomato was injured by 45%, with reduced heights, biomass, and yields; at 2.5 kg ae ha−1 injury of 8% and a biomass reduction was observed. Cucumber and squash were injured by 23% to 32% by glyphosate at 5.0 kg ae ha−1, with reductions in growth and early-season yield; lower rates did not influence crop growth or production when the crop was placed into a preexisting plant hole. Glufosinate applied at the same rates did not affect tomato growth or yield when planted into preexisting plant holes. Cucumber, when planted into preexisting plant holes, was injured by 43% to 75% from glufosinate, with reductions in height and biomass, and yield losses of 1.3 to 2.6 kg ai ha−1; similar results from glufosinate were observed in squash. In multi-crop plasticulture production, growers should ensure vegetable transplants are placed a minimum of 15 cm away from soil exposed to these herbicides.

Herbicide drift to sensitive crops can result in significant injury, yield loss, and even crop destruction. When pesticide drift is reported to the Georgia Department of Agriculture (GDA), tissue samples are collected and analyzed for residues. Seven field studies were conducted in 2020 and 2021 in cooperation with the GDA to evaluate the effect of (1) time interval between simulated drift event and sampling, (2) low-dose herbicide rates, and (3) the sample collection methods on detecting herbicide residues in cotton (Gossypium hirsutum L.) foliage. Simulated drift rates of 2,4-D, dicamba, and imazapyr were applied to non-tolerant cotton in the 8- to 9-leaf stage with plant samples collected at 7 or 21 d after treatment (DAT). During collection, plant sampling consisted of removing entire plants or removing new growth occurring after the 7-leaf stage. Visual cotton injury from 2,4-D reached 43% to 75% at 0.001 and 0.004 kg ae ha−1, respectively; for dicamba, it was 9% to 41% at 0.003 or 0.014 kg ae ha−1, respectively; and for imazapyr, it was 1% to 74% with 0.004 and 0.03 kg ae ha−1 rates, respectively. Yield loss was observed with both rates of 2,4-D (11% to 51%) and with the high rate of imazapyr (52%); dicamba did not influence yield. Herbicide residues were detected in 88%, 88%, and 69% of samples collected from plants treated with 2,4-D, dicamba, and imazapyr, respectively, at 7 DAT compared with 25%, 16%, and 22% when samples were collected at 21 DAT, highlighting the importance of sampling quickly after a drift event. Although the interval between drift event and sampling, drift rate, and sampling method can all influence residue detection for 2,4-D, dicamba, and imazapyr, the factor with the greatest influence is the amount of time between drift and sample collection.

In this study, we examined the impact of the number and type of arterial grafts, and surgical dressing type, on deep and organ/space surgical site infection following coronary artery bypass graft procedures. Bilateral internal mammary artery grafts and negative pressure wound therapy were associated with higher odds of infection.

Acute Appendicitis (AA) is an inflammatory condition of the vermiform appendix in the caecum of the colon. Genetic polymorphisms have been suggested as risk factors predisposing to AA susceptibility but have remained relatively unknown, due to insufficient sample size in previous analyses. Therefore, the primary research aim was to identify genetic variants associated with AA. It was hypothesised that gene polymorphisms associated with AA will provide a connection to other diet-related inflammatory diseases. Genetic variants associated with AA were studied via a Genome-Wide Association Scan (GWAS) using the Global Biobank Meta-Analysis Initiative (GBMI). The GBMI is a collaborative consortium of 23 biobanks with a publicly released repository of de-identified genetic data linked with digital health records spanning 4 continents with a study population size of over 2.2 million consented individuals of multiple ancestral backgrounds1. A linear regression model was used to estimate the association between single nucleotide polymorphisms (SNPs), across the human genome, and AA by each contributing biobank. The results were then meta-analysed with a total of 32,706 cases and 1,075,763 controls. In the present study, the free open-source Complex Traits Genetic Virtual Lab (CTG-VL) platform was used to access, analyse, and visualise the GWAS summary statistics of AA2. Genome-wide significantly associated SNPs (p-value < 5 x 10-8) were further searched for their associations with health-related traits in publicly available GWAS summary statistics. Upon analysis, significantly associated SNPs for AA were identified within or nearby nine genes. HLX, NKX2-3, LTBR, and DLEU1 are genes involved in immune responses; IRF8 associated with maturation of myeloid cells; OSR-1 responsible for transmembrane ion transporter activity; NCALD a regulator of G protein-coupled signal transduction. In addition, based on the hypothesis, the SNP of key clinical importance was the HLA-C rs2524046 (p-value = 2.38 x 10-8), with the AA risk-increasing allele C being also strongly associated with a higher risk of coeliac disease (CD). The CD is an autoimmune condition where gluten, a protein present in grains such as barley, rye, and wheat, elicits an inflammatory response that results in damage to the small intestine lining. Considering how both AA and CD share the same SNP, it is possible to speculate whether gluten initiates a similar pathophysiological mechanism that exacerbates inflammation in the vermiform appendix in AA. In conclusion, the top AA associated SNPs suggests its development could be due to immunological responses influenced by dietary nutrient intake. The HLA-C SNP is common to AA and CD, suggesting that the gluten protein found in certain cereal grains possibly contributes to the pathophysiology of AA like CD. This warrants further investigations into whether dietary gluten could play a key role in AA development.

Childhood obesity represents a significant global health concern and identifying its risk factors is crucial for developing intervention programs. Many “omics” factors associated with the risk of developing obesity have been identified, including genomic, microbiomic, and epigenomic factors. Here, using a sample of 48 infants, we investigated how the methylation profiles in cord blood and placenta at birth were associated with weight outcomes (specifically, conditional weight gain, body mass index, and weight-for-length ratio) at age six months. We characterized genome-wide DNA methylation profiles using the Illumina Infinium MethylationEpic chip, and incorporated information on child and maternal health, and various environmental factors into the analysis. We used regression analysis to identify genes with methylation profiles most predictive of infant weight outcomes, finding a total of 23 relevant genes in cord blood and 10 in placenta. Notably, in cord blood, the methylation profiles of three genes (PLIN4, UBE2F, and PPP1R16B) were associated with all three weight outcomes, which are also associated with weight outcomes in an independent cohort suggesting a strong relationship with weight trajectories in the first six months after birth. Additionally, we developed a Methylation Risk Score (MRS) that could be used to identify children most at risk for developing childhood obesity. While many of the genes identified by our analysis have been associated with weight-related traits (e.g., glucose metabolism, BMI, or hip-to-waist ratio) in previous genome-wide association and variant studies, our analysis implicated several others, whose involvement in the obesity phenotype should be evaluated in future functional investigations.

Herbicides that inhibit protoporphyrinogen oxidase (PPO) are used in more than 40 agronomic and specialty crops across Georgia to manage weeds through residual and postemergence (POST) control. In 2017, a population of Palmer amaranth exhibiting reduced sensitivity to POST applications of PPO-inhibiting herbicides was identified by the University of Georgia. Seed were collected from the site along with a known sensitive population; distance between the samples was 200 m, increasing the likelihood of similar environmental and genetic characteristics. To quantify sensitivity for both preemergence (PRE) and POST uses, 21 greenhouse dose-response assessments were conducted from 2017 to 2022. After conducting initial rate-response studies, 13 doses per herbicide were chosen for the POST experiment; field use rates of fomesafen (420 g ai ha−1), lactofen (219 g ai ha−1), acifluorfen (420 g ai ha−1), and trifludimoxazin (25 g ai ha−1) ranging from 0× to 4× the field use rate for the susceptible population, and 0× to 40× for the suspect population were applied. Herbicide treatments included adjuvants and were applied to plants 8 to 10 cm in height. Relative resistance factors (RRFs) were calculated for control ratings, mortality, and biomass, and ranged from 105 to 318, 36 to 1,477, 215 to 316, and 9 to 49 for fomesafen, lactofen, acifluorfen, and trifludimoxazin, respectively. In the PRE experiment, herbicide applications included five to nine doses of fomesafen (1× = 210 g ai ha−1), flumioxazin (1× = 57 g ai ha−1), oxyfluorfen (1× = 561 g ai ha−1), and trifludimoxazin (1× = 38 g ai ha−1); doses ranged from 0× to 6× for the suspect population and 0× to 2× for the susceptible population. Visual control, mortality, and biomass RRFs ranged from 3 to 5 for fomesafen, 21 to 31 for flumioxazin, 6 to 22 for oxyfluorfen, and 8 to 38 for trifludimoxazin. Results confirm that a Georgia Palmer amaranth population is resistant to PPO-inhibiting herbicides applied both PRE and POST.