Although historians have given close attention to the anti-vaccination movement that gripped late-Victorian England, relatively little scholarship explores how doctors and health officials responded or asks what strategies and assumptions structured how they might oppose the vaccine opponents. This article traces the advent and actions of the Jenner Society, a smallpox vaccination advocacy group founded in 1896 by Dr. Francis Bond. His goal was to publicly confront the leading anti-vaccinationists and to effectively conduct an anti-anti-vaccination campaign. The Jenner Society appeared amidst disputes over how and even whether vaccination should be publicly debated – disputes shaped both by long-standing attitudes toward professional propriety and also by indecision about what sorts of political advocacy were suitable for medical practitioners. Vaccination was shifting toward a more voluntary administration, and the Jenner Society represents how civil society, the popular press, and the modern tools of persuasion were becoming increasingly central to public health governance. The Jenner Society encapsulated the profession’s disdainful attitude toward populist medical dissent, and this essay argues that the tone and deportment of anti-anti-vaccinationism had the effect of encouraging doctors to overlook and neglect other, probably more significant, sources of vaccine skepticism. Preoccupied with rebutting and attacking vaccination’s enemies, public “controversialists” like Bond waged the first true large-scale pro-vaccination propaganda campaign, but they ultimately were unable to address the underlying dynamics of vaccine evasion. This history holds important lessons today for those interested in constructing more effective ways to effectively counteract medical misinformation and anti-vaccinationist beliefs.

Background: Standardized magnetic resonance imaging (MRI) guidelines published in 2015 by the Europoean MAGNIMS group and in 2016 by the CMSC are important for the diagnosis and monitoring of patients with multiple sclerosis (MS) and for the appropriate use of MRI in routine clinical practice. Methods: Two panels of experts convened to update existing guidelines for a standardized MRI protocol. The MAGNIMS panel convened in Graz, Austria in April 2019. The CMSC NAIMS panel met separately and independently in Newark, USA in October 2019. Subsequently, the MAGNIMS, NAIMS, and CMSC working groups combined their efforts to reach an international consensus Results: The revised guidelines on MRI in MS merges recommendations from MAGNIMS, CMSC, and NAIMS to improve the use of MRI for diagnosis, prognosis and monitoring of individuals with MS. 3D acquisitions are emphasized for optimal comparison over time. Core brain sequences include a 3D-T2wFLAIR for lesion identification and monitoring treatment effectiveness. Gadolinium-based contrast is recommended for diagnostic studies and judicious use for routine monitoring of MS patients. DWI sequences are recommended for PML safety monitoring. Conclusions: The international consensus guidelines strive for global acceptance of a useful and usable standard of care for patients with MS.

OBJECTIVES/GOALS: We seek to develop a 3D perfusion culture imaging plate for human fecal bacteria co-culture with epithelial cells in a structure that mimics the gut epithelium. We will develop this system for use with patient fecal samples to characterize patient risk of developing cancer. METHODS/STUDY POPULATION: E. coli NC101, a strain that harbors the pks gene island, produces the genotoxin colibactin which causes DNA damage that can lead to colorectal cancer development. The genotoxic ability of this bacterium is dependent upon cell-to-cell contact. Here, we present 3D printed E. coli NC101 and intestinal epithelial cells (IEC-6) in a perfusion imaging plate, enabling visualization of the cytotoxic effects of the bacteria in real time using confocal microscopy, in combination with flow cytometry analysis for cell cycle arrest (a surrogate marker of DNA damage). RESULTS/ANTICIPATED RESULTS: 40,000 IEC-6 cells were 3D printed in a cylindrical layer in our triple well imaging plate. The cells were infected at an MOI of 100 for 18 hours and time lapse images of the infection were recorded by confocal microscopy. The cells were then harvested for analysis by flow cytometry for cell cycle arrest as a measure of DNA damage. Our images and flow cytometry data show that E. coli NC101 co-localizes with IEC-6 cells and causes cell cycle arrest in phase G2 (infected %G2 = 40.1), compared to uninfected cells (%G2 = 24.7, P = 0.034). Mutant strains lacking adhesion protein FimH or the ability to produce colibactin do not cause G2 cell cycle arrest (P = 0.844 and P = 0.644, respectively). DISCUSSION/SIGNIFICANCE OF IMPACT: We are able to recapitulate the DNA damage phenotype of E. coli NC101 in our 3D culture system. We show here that host-microbe interactions leading to cancer can be modeled in our 3D perfusion system, and we will next use patient fecal samples in our culture system.

Greenhouse and field experiments were conducted to evaluate early postemergence (POST) tank mixtures of SAN 582 with fluazifop-P, imazethapyr, or sethoxydim. In the greenhouse, SAN 582 synergistically improved barnyardgrass, broadleaf signalgrass, and johnsongrass control from imazethapyr and sethoxydim. Half-rates of imazethapyr and sethoxydim tank-mixed with SAN 582 controlled grass weeds as well as full rates of either herbicide applied alone. Grass weed control with imazethapyr increased up to 40% with the addition of SAN 582. In field experiments, SAN 582 increased grass control with imazethapyr to a lesser degree than observed in the greenhouse. In a multispecies study, grass weed control increased up to 15% when SAN 582 was tank-mixed with a reduced rate of imazethapyr, although the full rate of imazethapyr applied POST with or without SAN 582 controlled grass weeds 80% or less. The combination of SAN 582 with sethoxydim was synergistic for barnyardgrass and johnsongrass control in this experiment. When applied POST in soybean, SAN 582 plus fluazifop-P or sethoxydim controlled barnyardgrass throughout the season better than a single POST application of a graminicide.

Outcome of moderate to severe traumatic brain injury (TBI) includes impaired emotion regulation. Emotion regulation has been associated with amygdala and rostral anterior cingulate (rACC). However, functional connectivity between the two structures after injury has not been reported. A preliminary examination of functional connectivity of rACC and right amygdala was conducted in adolescents 2 to 3 years after moderate to severe TBI and in typically developing (TD) control adolescents, with the hypothesis that the TBI adolescents would demonstrate altered functional connectivity in the two regions. Functional connectivity was determined by correlating fluctuations in the blood oxygen level dependent (BOLD) signal of the rACC and right amygdala with that of other brain regions. In the TBI adolescents, the rACC was found to be significantly less functionally connected to medial prefrontal cortices and to right temporal regions near the amygdala (height threshold T = 2.5, cluster level p < .05, FDR corrected), while the right amygdala showed a trend in reduced functional connectivity with the rACC (height threshold T = 2.5, cluster level p = .06, FDR corrected). Data suggest disrupted functional connectivity in emotion regulation regions. Limitations include small sample sizes. Studies with larger sample sizes are necessary to characterize the persistent neural damage resulting from moderate to severe TBI during development. (JINS, 2013, 19, 1–14)

Explosive blast is a frequent cause of traumatic brain injury (TBI) among personnel deployed to Afghanistan and Iraq. Functional magnetic resonance imaging (fMRI) with an event-related stimulus-response compatibility task was used to compare 15 subjects with mild, chronic blast-related TBI with 15 subjects who had not experienced a TBI or blast exposure during deployment. Six TBI subjects reported multiple injuries. Relative to the control group, TBI subjects had slightly slower responses during fMRI and increased somatic complaints and symptoms of post-traumatic stress disorder (PTSD) and depression. A between-group analysis indicated greater activation during stimulus-response incompatibility in TBI subjects within the anterior cingulate gyrus, medial frontal cortex, and posterior cerebral areas involved in visual and visual-spatial functions. This activation pattern was more extensive after statistically controlling for reaction time and symptoms of PTSD and depression. There was also a negative relationship between symptoms of PTSD and activation within posterior brain regions. These results provide evidence for increased task-related activation following mild, blast-related TBI and additional changes associated with emotional symptoms. Limitations of this study include no matching for combat exposure and different recruitment strategies so that the control group was largely a community-based sample, while many TBI subjects were seeking services. (JINS, 2012, 18, 89–100)

The present work has been done within the IAEA Environmental Modelling for Radiation Safety Programme. EMRAS-II aims to improve the capabilities in the field of environmental radiation dose assessment by means of acquisition of improved data for model testing, comparison, reaching consensus on modelling philosophies, approaches and parameter values, development of improved methods and exchange of information.

In January 2009, the IAEA EMRAS II (Environmental Modelling for Radiation Safety II) program was launched. The goal of the program is to develop, compare and test models for the assessment of radiological impacts to the public and the environment due to radionuclides being released or already existing in the environment; to help countries build and harmonize their capabilities; and to model the movement of radionuclides in the environment. Within EMRAS II, nine working groups are active; this paper will focus on the activities of Working Group 1: Reference Methodologies for Controlling Discharges of Routine Releases. Within this working group environmental transfer and dose assessment models are tested under different scenarios by participating countries and the results compared. This process allows each participating country to identify characteristics of their models that need to be refined. The goal of this working group is to identify reference methodologies for the assessment of exposures to the public due to routine discharges of radionuclides to the terrestrial and aquatic environments. Several different models are being applied to estimate the transfer of radionuclides in the environment for various scenarios. The first phase of the project involves a scenario of nuclear power reactor with a coastal location which routinely (continuously) discharges 60Co, 85Kr, 131I, and 137Cs to the atmosphere and 60Co, 137Cs, and 90Sr to the marine environment. In this scenario many of the parameters and characteristics of the representative group were given to the modellers and cannot be altered. Various models have been used by the different participants in this inter-comparison (PC-CREAM, CROM, IMPACT, CLRP POSEIDON, SYMBIOSE and others). This first scenario is to enable a comparison of the radionuclide transport and dose modelling. These scenarios will facilitate the development of reference methodologies for controlled discharges.

A paleodietary analysis of the mid-Holocene mortuary site, Windover (8BR246), based on carbon and nitrogen bone-collagen values and archaeobotanical information is consistent with a subsistence strategy that utilized river-dwelling fauna and a range of terrestrial flora, such as grapes and prickly pear. The isotopic analysis does not support the extensive human dietary use of either marine mammals or classic terrestrial fauna such as deer or rabbit. Seasonal (late summer/early fall) use of the site is indicated by the range of flora found in association with the burials.

The Suazoid period lasted some three hundred years (c. AD 1100–1400) in a small geographic area in the Lesser Antilles from Tobago to Southern Martinique. Defined by a relatively narrow ceramic series, the people represented by this material are still poorly understood. Deriving from peoples from mainland South America, they evolved a marine-based settlement area of some 35,000 square miles including six main islands and many smaller ones. This paper discusses the economic basis for one Suazoid group based on evidence from test-pitting an extensive site at Heywoods, Barbados. Formerly a mangrove swamp, the site yielded extensive evidence for a marine-based economy. In addition, the first evidence on Barbados for an Archaic presence (c. 2000 BC) was located, together with Saladoid (c. 2.00 BC–AD 650) and Troumassoid (c. AD 650–1100) activity.