This study investigates the influence of workplace conditions on job satisfaction, focusing on environmental, occupational safety, and social factors, paying particular attention to gender interactions. Drawing on the European Survey on Workplace Health, Wellbeing, and Quality of Work Life, data from 514 employees in local companies and public organizations across six Southern European countries were analysed using discrete choice regression models. The empirical findings identify ventilation, ergonomics, social spaces, and safety training as the strongest positive drivers of job satisfaction, while repetitive work negatively affects it. The results show that women, highly educated employees, and those with permanent contracts report higher job satisfaction, with female workers benefiting most from ergonomic improvements and safety equipment. Managers should therefore prioritize improvements in workplace conditions – particularly ventilation, ergonomics, safety training, and job stability – while integrating gender-sensitive approaches to strengthen both employees’ well-being and organisational performance.