Recent changes to US research funding are having far-reaching consequences that imperil the integrity of science and the provision of care to vulnerable populations. Resisting these changes, the BJPsych Portfolio reaffirms its commitment to publishing mental science and advancing psychiatric knowledge that improves the mental health of one and all.

Calyptosuchus wellesi is an aetosaur known from the upper Blue Mesa Member and lowermost Sonsela Member of the Chinle Formation in Arizona, and the Tecovas Formation of the Dockum Group of Texas. The taxon is considered biostratigraphically informative and aids in correlating Upper Triassic strata across the southwestern United States. Our anatomical understanding of Calyptosuchus was primarily based on the holotype specimen from Texas and referred specimens, including several disarticulated elements from the Placerias Quarry in northern Arizona. We provide a re-interpretation of the holotype specimen and describe two new specimens referrable to Calyptosuchus from northern Arizona. Together these specimens provide new information on the cranial anatomy, including dentition, and the positional and intraspecific variation within the carapace and vertebral column of the taxon. This new evidence suggests that the skull anatomy of Calyptosuchus is more similar to that of Neoaetosauroides and Stagonolepis than to other aetosaurs. The dentition of Calyptosuchus supports recent hypotheses suggesting that aetosaurs were more omnivorous/faunivorous. The associated carapace expands our anatomical understanding of characters that vary among trunk paramedian osteoderms. We identify variation in the vertebral column within the trunk region, including the prominence of the centrodiapophyseal lamina and anatomy of the neural spine. Additionally, we report the first documentation of co-ossified sacral vertebrae within Calyptosuchus, which is also the first observation of this state within the Aetosauria outside of the Desmatosuchini. This suggests that co-ossification of the sacral vertebrae may be more prevalent within the Aetosauria than previously recognized.

http://zoobank.org/da3735b8-edf1-465d-820a-316174ed7bf9

Objectives/Goals: Early childhood obesity is a major concern for Latin American children in the U.S., with gut barrier dysfunction as a key risk factor. Diet plays a role in gut development, but few studies have focused on Latin American infants. Our objective is to identify culturally relevant introductory foods that promote in vitro gut barrier development and function. Methods/Study Population: Pooled human milk (2.5 mL) from 6-month postpartum Hispanic mothers was combined with fruit and vegetable baby food products (2.5 g) and subjected to a 3-phase in vitro digestion system that simulates oral, gastric, and intestinal digestion. Digesta products were then anaerobically fermented for 24-hours using human stool inoculum, centrifuged, and filter sterilized. Intestinal epithelial cells (Caco-2, ATCC) were grown to confluence on 0.4 μm polystyrene transwell inserts using a DMEM + 10% FBS medium and allowed to differentiate for 21-days. Highly differentiated monolayers were treated with a 1:4 dilution of fermenta with medium in triplicate. The cell experiment was conducted twice. Cell layer integrity was measured using transepithelial electrical resistance (TEER) 24- and 48-hours after treatment. Results/Anticipated Results: Dietary intake data from the What We Eat in America database indicated that the top 3 fruit and vegetable exposures for infants with Mexican or Hispanic ethnicity were banana, apple, and carrot. Commercial baby food purees of these fruits and vegetables, in addition to baby foods with blueberry and spinach (Natural for Baby, Gerber Products Company) were acquired for digestion and fermentation experiments. Caco-2 cell experiments with these foods are ongoing. We expect Caco-2 monolayer incubated with fermenta from human milk and fruit or vegetables will have greater TEER values due to increased integrity of the cell layer as compared to those with breast milk alone. We also expect that exposure to fruit and vegetable fermenta will increase gene expression of tight junctions compared to exposure to media and human milk. Discussion/Significance of Impact: Using an in vitro digestion and fermentation system coupled with cell culture studies, we are identifying cellular mechanisms that link individual fruits and vegetables to gut barrier function. This will support translational work focused on mitigating obesity development in vulnerable populations.

Residual herbicides are primarily degraded in the soil through microbial breakdown. Any practices that result in increased soil biological activity, such as cover cropping (between cash crop seasons), could lead to a reduced persistence of herbicides in the soil. Furthermore, cover crops can also interfere with herbicide fate by interception. Field trials were conducted between 2020 and 2023 in a corn (Zea mays L.)–soybean [Glycine max (L.) Merr.] rotation to investigate the influence of cover crop (cereal rye [Secale cereale L.] and crimson clover [Trifolium incarnatum L.]) use on soil enzyme activities (β-glucosidase [BG] and dehydrogenase [DHA]), its effect on the concentration of residual herbicides (sulfentrazone, S-metolachlor, cloransulam-methyl, atrazine, and mesotrione) in the soil, and the interception of herbicides by cover crop residue. The use of cover crops occasionally resulted in increased BG and DHA activities relative to the fallow treatment. However, even when there was an increase in the activity of these two enzymes, increased degradation of the residual herbicides was not observed. The initial concentrations of all residual herbicides in the soil were significantly reduced due to interception by cereal rye biomass. Nevertheless, significant reductions in early-season weed biomass were observed when residual herbicides were included in the tank mixture applied at cover crop termination relative to the application of glyphosate plus glufosinate. Results from this research suggest that the use of cereal rye or crimson clover as cover crops (between cash crop seasons) do not impact the persistence of residual herbicides in the soil or reduce their efficacy in controlling weeds early in the growing season.

Field studies were conducted on certified organic land in Lafayette and Vincennes, IN, in 2023 to determine the impact of different between-row weed control methods on weed suppression and sweetpotato yield. Between-row treatments consisted of organic buckwheat (108 kg ha−1) broadcast seeded immediately after sweetpotato transplanting followed by silage tarping from 3 wk after transplanting (WATr) through harvest, organic buckwheat (108 kg ha−1) broadcast seeded 3 WATr and terminated 7 WATr, and cultivation as a grower standard. Weed density at 6 WATr was 0, 184, and 162 plants m−2 for the silage tarping, living mulch buckwheat, and cultivation treatments, respectively. Total yield was 11,048 kg ha−1 for the living mulch buckwheat, 19,792 kg ha−1 for the cultivation, and 17,814 kg ha−1 for the tarping treatments. Tarping effectively suppressed weeds and produced sweetpotato yields comparable to cultivation, indicating the potential for use by organic growers. When buckwheat was grown between rows 3 to 7 WATr, sweetpotato yield was lower than it was with tarping and cultivation. These results suggest that researchers should be evaluating tarps for small-acreage farmers as a weed management strategy.

The battles over official secrecy have to be fought on many different fronts. In the United Kingdom—whose ministers and public servants allegedly have a “passion for secrecy”—this was more than usually evident during 1967. Two pieces of legislation—namely, the Parliamentary Commissioner Act, 1967 (U.K.) and the Public Records Act, 1967 (Eng.)—represented a modest triumph for those who would wish to see more light shed upon the affairs of the nation. Elsewhere the picture was rather more familiar. The Security Commission recommended a further tightening of documentary security in the Cabinet Office; this followed upon the conviction under the Official Secrets Acts, 1911 to 1939 (U.K.) of a young typist in the Cabinet Office, “the first known occasion on which classified papers have been extracted from” this inner sanctum of government.

Organic sweetpotato growers have limited effective weed management options, and most rely on in-season between-row cultivation and hand weeding, which are time consuming, are costly, and deteriorate soil quality. Studies were conducted at the Samuel G. Meigs Horticulture Research Farm, Lafayette, IN, and at the Southwest Purdue Agricultural Center, Vincennes, IN, in 2022 and 2023 to determine the effects of in-row plant spacing and cultivar selection on weed suppression and organic sweetpotato yield. The experiment was a split-split plot design, with in-row spacings of 20, 30, and 40 cm as the main plot factor, weeding frequency (critical weed-free period and weed-free) as the subplot factor, and sweetpotato cultivar (‘Covington’ and ‘Monaco’) as the sub-subplot factor. However, in 2022, we evaluated only in-row spacing and weeding frequency because of the poor establishment of ‘Monaco’. In 2023, sweetpotato canopy at 5 wk after transplanting (WAP) decreased as in-row spacing increased from 20 to 40 cm, and sweetpotato canopy cover of ‘Monaco’ (62%) was greater than that of ‘Covington’ (44%). In-row spacing did not affect weed density at 4, 5, and 6 WAP. As in-row spacing increased from 20 to 40 cm, total sweetpotato yield pooled across both locations in 2023 decreased from 30,223 to 21,209 kg ha−1 for ‘Covington’ and from 24,370 to 20,848 kg ha−1 for ‘Monaco’; however, jumbo yield increased for both cultivars. Findings from this study suggest that an in-row spacing of 20 cm may provide greater yield than the standard spacing of 30 cm for both ‘Monaco’ and ‘Covington’.

The authors report on ancient DNA data from two human skeletons buried within the chancel of the 1608–1616 church at the North American colonial settlement of Jamestown, Virginia. Available archaeological, osteological and documentary evidence suggest that these individuals are Sir Ferdinando Wenman and Captain William West, kinsmen of the colony's first Governor, Thomas West, Third Baron De La Warr. Genomic analyses of the skeletons identify unexpected maternal relatedness as both carried the mitochondrial haplogroup H10e. In this unusual case, aDNA prompted further historical research that led to the discovery of illegitimacy in the West family, an aspect of identity omitted, likely intentionally, from genealogical records.

The crystallite sizes in the particles from four fractions of a kaolinite-clay were determined from the broadening of the X-ray diffraction lines. Measurements were made of the <002> and <111> planes whose crystallographic directions correspond to the clay plate thickness and diagonal, respectively. The extent of crystal imperfection was determined by comparing the calculated crystallite size with the mean size based on measurements from electron micrographs. The crystal imperfections were found to be more extensive in the plate diagonal, <111>, than in the plate face, <002>, directions. Electron micrographs of hydrofluoric acid-etched samples revealed plate-edge and plate-face imperfections. The latter show a regularity suggesting a mosaic-like texture in the plate surface. Surface imperfections probably have significant influence on the dispersion and flocculation behavior of kaolinite.

The Tulameen coal field is part of an Eocene nonmarine basin which received extensive volcaniclastic sediments due to its location within an active magmatic arc. Bentonite partings in the coal originally consisted of glassy rhyolitic tephra with phenocrysts of sanidine, biotite, and quartz. During the initial alteration, which took place within the swamp or shortly after burial, glass was transformed to either smectite-cristobalite-clinoptilolite or to smectite-kaolinite. The formation of kaolinite depended on the degree of leaching of silica and alkalies in the swamp environment. Some beds are nearly 100% kaolinite and can be designated as tonsteins. The smectite shows no evidence of interlayering; the kaolinite is well ordered. During alteration, sodium, originally a component of the glass, was lost from the system.

A later thermal event, which affected only the southern part of the basin, metamorphosed the smectite to a regularly interstratified illite/smectite with 55% illite layers and rectorite-type superlattice (IS-type). The source of potassium was dissolution of sanidine. Vitrinite reflectance measurements of the coal suggest that the smectite was stable to 145–160°C, at which temperature it transformed to K-rectorite.

The absence of randomly interstratified intermediates, even in beds rich in potassium, suggests that the transformation of smectite to K-rectorite was controlled by a steep thermal gradient possibly resulting from local magmatism or circulating geothermal fluids.

A variational principle is proposed to derive the governing equations for the problem of ocean wave interactions with a floating ice shelf, where the ice shelf is modelled by the full linear equations of elasticity and has an Archimedean draught. The variational principle is used to form a thin-plate approximation for the ice shelf, which includes water–ice coupling at the shelf front and extensional waves in the shelf, in contrast to the benchmark thin-plate approximation for ocean wave interactions with an ice shelf. The thin-plate approximation is combined with a single-mode approximation in the water, where the vertical motion is constrained to the eigenfunction that supports propagating waves. The new terms in the approximation are shown to have a major impact on predictions of ice shelf strains for wave periods in the swell regime.

OBJECTIVES/GOALS: To determine in vitro mechanisms by which fruits and vegetables (FV) contribute to colon barrier development in Latin American infants. We hypothesize that simulated colonic fermentation of FVs will stimulatein vitro cell barrier function by activating the hypoxia-inducible factor (HIF) pathway in colonocytes. METHODS/STUDY POPULATION: FVs consumed by US-based Latin American infants 6-12 months old (identified from NHANES-What We Eat in America Surveys) will be combined with human breast-milk samples from women self-identified as Hispanic or non-Hispanic, and then subjected to in vitro digestion and anaerobic colonic fermentation using human feces. FV fermenta will be incubated with Caco2 monolayers to measure in vitro cell permeability and protein levels of cellular tight junction, metabolic, and HIF signaling enzymes. To examine their effects in vivo, FVs identified to modulate in vitro barrier function, will be fed (5% freeze dried powder) to wild-type mice and the above parameters will be examined. If in vivo effects are found, intestinal specific HIF knockout mice will be used to examine the role of HIF signaling in mediating these effects. RESULTS/ANTICIPATED RESULTS: We expect that fermenta derived from human milk and FVs will reduce in vitro gut permeability in Caco2 monolayers by increasing gene and protein expression of the HIF signaling complex relative to fermenta of human milk alone. This will be reflected with higher cellular trans-epithelial resistance and greater expression levels of tight junction proteins. We expect FV powder consumption will similarly increase in vivo gut permeability and expression of related genes in mice as compared to mice fed diets without FVs. As we expect an increase in HIF signaling in the colon, we expect that FV powder consumption will not enhance in vivo gut permeability in mice colons with an intestinal specific knockout of HIF. DISCUSSION/SIGNIFICANCE: Data from this study will provide mechanistic evidence to help clinicians promote relevant FVs recommendations for Latin American infants and families. Due to the link between gut permeability and obesity, our next step will be to conduct a dietary intervention in this population.

We report the discovery of a bow-shock pulsar wind nebula (PWN), named Potoroo, and the detection of a young pulsar J1638$-$4713 that powers the nebula. We present a radio continuum study of the PWN based on 20-cm observations obtained from the Australian Square Kilometre Array Pathfinder (ASKAP) and MeerKAT. PSR J1638$-$4713 was identified using Parkes radio telescope observations at frequencies above 3 GHz. The pulsar has the second-highest dispersion measure of all known radio pulsars (1 553 pc cm$^{-3}$), a spin period of 65.74 ms and a spin-down luminosity of $\dot{E}=6.1\times10^{36}$ erg s$^{-1}$. The PWN has a cometary morphology and one of the greatest projected lengths among all the observed pulsar radio tails, measuring over 21 pc for an assumed distance of 10 kpc. The remarkably long tail and atypically steep radio spectral index are attributed to the interplay of a supernova reverse shock and the PWN. The originating supernova remnant is not known so far. We estimated the pulsar kick velocity to be in the range of 1 000–2 000 km s$^{-1}$ for ages between 23 and 10 kyr. The X-ray counterpart found in Chandra data, CXOU J163802.6$-$471358, shows the same tail morphology as the radio source but is shorter by a factor of 10. The peak of the X-ray emission is offset from the peak of the radio total intensity (Stokes $\rm I$) emission by approximately 4.7$^{\prime\prime}$, but coincides well with circularly polarised (Stokes $\rm V$) emission. No infrared counterpart was found.

Sweetpotato [Ipomoea batatas (L.) Lam.] is a staple crop that provides nutritional benefits to humans globally, but it is subject to yield loss when competing with weeds, especially during the early stage of establishment. Yield loss can vary widely based on the cultivar, production environment, weed species, and management techniques. To address this challenge, we conducted field research at the Samuel G. Meigs Horticulture Research Farm, Lafayette, IN, and at the Southwest Purdue Agricultural Center, Vincennes, IN, in 2022 to determine the effect of sweetpotato cultivar on the critical weed-free period. The experiment was a split-plot design, with weed-free interval treatments as the main plot factor and cultivar as the subplot factor. The three cultivars used were ‘Covington’, ‘Monaco’, and ‘Murasaki’. Weeds were removed by hand and allowed to establish and compete with the crop beginning at 0, 14, 21, 28, 35, or 42 d after transplanting (DAP). As the weed-free interval increased from 0 to 42 DAP, predicted total yield increased from 19 kg ha−1 to 20,540 kg ha−1 for Covington, 3 kg ha−1 to 11,407 kg ha−1 for Monaco, and 125 kg ha−1 to 13,460 kg ha−1 for Murasaki at the Lafayette location. At Vincennes, as the weed-free interval increased from 0 to 42 DAP, predicted total yield increased from 14,664 kg ha−1 to 33,905 kg ha−1 for Covington, 4,817 kg ha−1 to 18,059 kg ha−1 for Monaco, and 12,735 kg ha−1 to 21,105 kg ha−1 for Murasaki. A threshold of ≤10% total yield reduction was achieved by maintaining sweetpotatoes weed-free 24 DAP for Covington, 20 DAP for Murasaki, and 33 DAP for Monaco.

The swelling of n-butylammonium vermiculite in water was investigated as a function of the sol concentration (r), the salt concentration (c) and the temperature (T).

The interlayer spacing in the gel phase was investigated as a function of r and c by neutron diffraction and by laboratory experiments which measured how many times its own volume a crystal would absorb. The salt concentration was found to be the stronger variable with the interlayer spacing decreasing proportional to c0.5, which is consistent with previous results and with the Coulombic attraction theory. The sol concentration was found to affect the swelling for two reasons, the salt fractionation effect and the trapped salt effect. Both of these cause the salt concentration in the supernatant fluid to be greater than that originally added to the crystals and so reduce the swelling.

A new method was used for extracting the solution from inside the gels by collapsing the gels by the addition of potassium hydrogen carbonate. The Volhardt titration was carried out on the extracted and supernatant solutions from about 250 gels. The ratio of the external to the internal chloride concentration was found to be approximately constant across the range of salt concentrations. Its average value was equal to 2.6, again in agreement with Coulombic attraction theory and showing the surface potential to be constant at about 70 mV.

The (r, c, T) boundary of the two phase colloid region was investigated by three methods. A plot of log c against Tc was linear within experimental accuracy, with a gradient of 0.077 K−1 or 13 K per log unit. This shows that the surface potential varies by only 1 mV per decade in the salt concentration. The system is therefore governed by the Dirichlet boundary condition and not by the Nernst equation.

We present a demonstration version of a commensal pipeline for Fast Radio Burst (FRB) searches using a real-time incoherent beam from the Murchison Widefield Array (MWA). The main science target of the pipeline are bright nearby FRBs from the local Universe (including Galactic FRBs like from SGR 1935+2154) which are the best candidates to probe FRB progenitors and understand physical mechanisms powering these extremely energetic events. Recent FRB detections by LOFAR (down to 110 MHz), the Green Bank Telescope (at 350 MHz), and Canadian Hydrogen Intensity Mapping Experiment (CHIME) detections extending down to 400 MHz, prove that there is a population of FRBs that can be detected below 350 MHz. The new MWA beamformer, known as the ‘MWAX multibeam beamformer’, can form multiple incoherent and coherent beams (with different parameters) commensally to any ongoing MWA observations. One of the beams is currently used for FRB searches (tested in 10 kHz frequency resolution and time resolutions between 0.1 and 100 ms). A second beam (in 1 Hz and 1 s frequency and time resolutions, respectively) is used for the Search for Extraterrestrial Intelligence (SETI) project. This paper focuses on the FRB search pipeline and its verification on selected known bright pulsars. The pipeline uses the FREDDA implementation of the Fast Dispersion Measure Transform algorithm (FDMT) for single pulse searches. Initially, it was tested during standard MWA observations, and more recently using dedicated observations of a sample of 11 bright pulsars. The pulsar PSR J0835-4510 (Vela) has been routinely used as the primary probe of the data quality because its folded profile was always detected in the frequency band 200 – 230 MHz with typical signal-to-noise ratio $>$10, which agrees with the expectations. Similarly, the low dispersion measure pulsar PSR B0950+08 was always detected in folded profile in the frequency band 140–170 MHz and so far has been the only object for which single pulses were detected. We present the estimated sensitivity of the search in the currently limited observing bandwidth of a single MWA coarse channel (1.28 MHz) and for the upgraded, future system with 12.8 MHz (10 channels) of bandwidth. Based on expected sensitivity and existing FRB rate measurements, we project an FRB detection rate between a few and a few tens per year with large uncertainty due to unknown FRB rates at low frequencies.