This paper presents the radiocarbon context of the megalithic monument El Amarejo 1, situated in the corridor of Almansa in the southern region of La Meseta in Spain. The monument was constructed using small and medium-sized masonry, comprising a short corridor and two separate chambers in which burials were carried out. The results of the 14C analyses of each of the 11 individuals documented indicate that the monument was in use between approximately 1900 and 1200 cal BC. Bayesian modeling of the radiocarbon dates allows for the proposition of hypotheses regarding the construction, utilisation dynamics, and abandonment of the monument. The combination of these new data with the analysis of the 14C dating of other burials from the Bronze Age of La Mancha reveals a complex and heterogeneous panorama. The evidence presented and analyzed in this paper suggests that burial practices associated with fortified settlements and their domestic areas shared space and time with the construction of megalithic monuments located near settlements.

The relevance of education and outreach (E&O) activities about the Antarctic Treaty has been recognized at the Antarctic Treaty Consultative Meetings (ATCM) and at the Committee for Environmental Protection (CEP). This study examines the key topics and the target audiences detailed in papers submitted to the ATCM on E&O. Since the Antarctic Treaty entered into force in 1961, a total of 216 ATCM papers on E&O have been produced. The number of papers has increased substantially since the mid-1990s. ‘Science’ (76.9%) and ‘Wildlife/Biodiversity/Environment’ (75.5%) were the most addressed topics in these papers, while the ‘Public’ (81.0%) and those attending ‘Schools’ (69.0%) are the main target audiences. ‘Science’ in ATCM papers increased ~120-fold from 1961–1997 to 2015–2023, while ATCM papers discussing engagement with the ‘Public’ increased ~40-fold during the same period. ‘Climate change’ was first mentioned in 2006, and the number of papers per year increased fourfold by 2015–2023. This study shows the increasing interest in E&O through time, addressing key topics to relevant audiences related to the Antarctic region. From an educational perspective, attention should be paid to emerging topics (e.g. equity, diversity and inclusion), and the engagement of early-career professionals and educators should be made a priority.

PEPAdb (Prehistoric Europe's Personal Adornment Database) is a long-term, open-ended project that aims to improve access to archaeological data online. Its website (https://pepadb.us.es) publishes and analyses datasets about prehistoric personal adornment, drawing on the results of various research projects and bibliographic references.

A magnetic composite was prepared by wet-impregnating a powder of a natural zeolite with a magnetic Fe oxide-containing synthetic material. Both starting materials were first characterized with X-ray diffraction, scanning electron microscopy, Mössbauer spectroscopy, and by isoelectric-point using vibrating-sample magnetometry. The synthetic Fe oxide-containing material was characterized as a mixture of magnetite (Fe3O4) and goethite (α-FeOOH). From the Fe Mössbauer analysis, the relative subspectral area for magnetite corresponds to 93(2)%; the remaining spectrum is assignable to goethite. After the impregnation process, magnetite was still identified in the composite material as a magnetic layer surrounding the zeolite particles; no magnetically ordered goethite could be detected. The Mössbauer pattern for this sample indicates a much more complex structure than for the precursor material, based on Fe oxides, with some more altered magnetite and an intense central doublet of (super)paramagnetic Fe3+, probably due to small Fe (hydr)oxides and/or to a residual contribution of Fe-bearing species from the starting zeolite material. The composite preparation procedure also promoted the change of the characteristic A-type zeolite to mordenite. The resulting magnetic composite presented a magnetic coercivity of as much as 0.140 A m−1, at 77 K. The final composite is now being evaluated as an adsorbent: results to date confirm that this novel magnetic material may have applications in the remediation of contaminated water bodies.

Image-processing pipelines require the design of complex workflows combining many different steps that bring the raw acquired data to a final result with biological meaning. In the image-processing domain of cryo-electron microscopy single-particle analysis (cryo-EM SPA), hundreds of steps must be performed to obtain the three-dimensional structure of a biological macromolecule by integrating data spread over thousands of micrographs containing millions of copies of allegedly the same macromolecule. The execution of such complicated workflows demands a specific tool to keep track of all these steps performed. Additionally, due to the extremely low signal-to-noise ratio (SNR), the estimation of any image parameter is heavily affected by noise resulting in a significant fraction of incorrect estimates. Although low SNR and processing millions of images by hundreds of sequential steps requiring substantial computational resources are specific to cryo-EM, these characteristics may be shared by other biological imaging domains. Here, we present Scipion, a Python generic open-source workflow engine specifically adapted for image processing. Its main characteristics are: (a) interoperability, (b) smart object model, (c) gluing operations, (d) comparison operations, (e) wide set of domain-specific operations, (f) execution in streaming, (g) smooth integration in high-performance computing environments, (h) execution with and without graphical capabilities, (i) flexible visualization, (j) user authentication and private access to private data, (k) scripting capabilities, (l) high performance, (m) traceability, (n) reproducibility, (o) self-reporting, (p) reusability, (q) extensibility, (r) software updates, and (s) non-restrictive software licensing.

This systematic literature review aimed to provide an overview of the characteristics and methods used in studies applying the disability-adjusted life years (DALY) concept for infectious diseases within European Union (EU)/European Economic Area (EEA)/European Free Trade Association (EFTA) countries and the United Kingdom. Electronic databases and grey literature were searched for articles reporting the assessment of DALY and its components. We considered studies in which researchers performed DALY calculations using primary epidemiological data input sources. We screened 3053 studies of which 2948 were excluded and 105 studies met our inclusion criteria. Of these studies, 22 were multi-country and 83 were single-country studies, of which 46 were from the Netherlands. Food- and water-borne diseases were the most frequently studied infectious diseases. Between 2015 and 2022, the number of burden of infectious disease studies was 1.6 times higher compared to that published between 2000 and 2014. Almost all studies (97%) estimated DALYs based on the incidence- and pathogen-based approach and without social weighting functions; however, there was less methodological consensus with regards to the disability weights and life tables that were applied. The number of burden of infectious disease studies undertaken across Europe has increased over time. Development and use of guidelines will promote performing burden of infectious disease studies and facilitate comparability of the results.

OBJECTIVES/GOALS: Research Education Towards Opportunities (RETO) Mentoring Offering Training Opportunities for Research (MOTOR) 1 – 2 and the elective courses (INTD 5998/ MDCL 101) in CTR of Title V Coop were designed to provide the participants from higher education institution (HEI) in Puerto Rico (PR), interdisciplinary – interprofesional knowledge in CTR. METHODS/STUDY POPULATION: Since April 2017, Research Education Towards Opportunities (RETO) and Mentoring Offering Training Opportunities for Research (MOTOR) 1 - 2 trainings were offered as part of the Title V Coop. In addition, since January 2020, as part of the institutionalization of the trainings in CTR, two elective courses (INTD 5998 and MDCL 101) were created-offered. The trainings/courses present the main concepts underlying CTR performance through lectures, workshops and presentations, in hybrid modalities, as well as the services-resources of the Center Research Education and Science Commnunication Opportunities (CRESCO). These programs have given students (undergraduate and graduate) and faculty the opportunity to get started in CTR and to integrate in Clinical and Translational Mentoring Teams (CTMT). RESULTS/ANTICIPATED RESULTS: Eight (8) cycles of RETO-MOTOR 1 and seven (7) cycles of RETO-MOTOR 2; two sessions of INTD 5998 and one session of MDCL 101 were offered. The RETO-MOTOR 1 training was completed by 219 participants and RETO-MOTOR 2 by 130 participants. The INTD 5998 course was completed by 22 students and the MDCL 101 course by 18 students. A total of 389 participants have been initiated in the CTR. Of the trainings, 90% indicated that the knowledge acquired in CTR was invaluable, 85% understand that the most significant achievement, as students, was present at a scientific conference, and 100% indicated interest in continuing to do CTR. Of the courses, 100% indicated that they were a good learning experience, helped them increase their knowledge in CTR, met their expectations and would recommend other students to take the course. DISCUSSION/SIGNIFICANCE: The RETO-MOTOR 1, RETO-MOTOR 2 trainings and CTR courses provide a based of research knowledge and valuable interprofessional experience for those who whish to start in the clinical and translational research. The Title V Cooperative Project provides this opportunity to undergraduate and graduate students such as faculty of HEI in PR.

This article argues that a holistic approach to documenting and understanding the physical evidence for individual cities would enhance our ability to address major questions about urbanisation, urbanism, cultural identities and economic processes. At the same time we suggest that providing more comprehensive data-sets concerning Greek cities would represent an important contribution to cross-cultural studies of urban development and urbanism, which have often overlooked relevant evidence from Classical Greece. As an example of the approach we are advocating, we offer detailed discussion of data from the Archaic and Classical city of Olynthos, in the Halkidiki. Six seasons of fieldwork here by the Olynthos Project, together with legacy data from earlier projects by the American School of Classical Studies at Athens and by the Greek Archaeological Service, combine to make this one of the best-documented urban centres surviving from the Greek world. We suggest that the material from the site offers the potential to build up a detailed ‘urban profile’, consisting of an overview of the early development of the community as well as an in-depth picture of the organisation of the Classical settlement. Some aspects of the urban infrastructure can also be quantified, allowing a new assessment of (for example) its demography. This article offers a sample of the kinds of data available and the sorts of questions that can be addressed in constructing such a profile, based on a brief summary of the interim results of fieldwork and data analysis carried out by the Olynthos Project, with a focus on research undertaken during the 2017, 2018 and 2019 seasons.

This study focuses on the dolphins populating the water between Gibraltar and Algeciras in the south Iberian Peninsula, an area subjected to pressure due to high human activity. The area is considered an important feeding and breeding ground for common dolphins (Delphinus delphis). Due to the degree of residence of some specimens, and the large gap in knowledge about the evolution of wounds in D. delphis specimens with lacerations, this work sought to perform the following analyses: identify lacerated individuals; characterize sequences of ‘before – during – after’ with respect to the occurrence of lacerations; and associate the type of injury with its severity. This work will inform future studies by expanding a database on injured individuals and contribute to periodical monitoring of specimens that frequent these geographic areas. Between 2013 and 2017, we were able to track the healing process of five injured individuals of common dolphins from a whale-watching platform thanks to photo identification. The animals exhibited fresh external wounds from different sources. In the majority of individuals, the wound-healing processes lasted 3–21 weeks. The frequency with which sightings are made and knowledge about the local population will help track injured animals, follow their wound evolution, and document their survival rates. The documented injuries inflicted by human interactions described in this paper may include fishing interactions and propeller strikes, probably as a consequence of the high intensity of recreational fishing and whale-watching activities in the area.

OBJECTIVES/SPECIFIC AIMS: Responding to the need and interest of students and faculty of the UHSP in learning about CTR, the Title V Cooperative Project between UPR-MSC and UCC, developed and offered a training cycle (TC) in CTR. METHODS/STUDY POPULATION: Undergraduate students (US), undergraduate faculty (UF), and graduate students (GS) were invited to register in: Research Education Towards Opportunities (RETO) and Mentorship Offering Training Opportunities for Research (MOTOR), which consisted of 20 hours of training in CTR, with interdisciplinary sessions in: Introduction and preparation of a presentation in CTR; Identify, interview and share a presentation of a CT researcher; participation in conferences and a summer camp in CTR. At the end of the TC, surveys—satisfaction and needs assessment—for training in CTR were administered. RESULTS/ANTICIPATED RESULTS: Thirty-three (33) registered in the TC, distributed: 13 (39.39%) US in RETO, 12 (36.36%) GS and 8 (24.24%) UF in MOTOR. Of these, 25 (75.75%) answered and submitted the on-line surveys and received a completion certificate. All (100%) were satisfied with the TC, and for 96% of the respondents, their expectations were fulfilled, and will continue in the TC. They selected critical review, scientific communication, and cultural diversity as thematic areas of interest. In addition, 60% of them selected neuroscience, cancer and medical imaging as main research areas of interest. DISCUSSION/SIGNIFICANCE OF IMPACT: The TC demonstrated to be an effective strategy to provide new knowledge, experiences, and interest in CTR. It also established a pathway for future engagement in CTR.

Alien invasive species have strategies that can maintain fitness in a variety of environments. This flexibility is associated with environmental tolerance in several traits, such as allocation of resources to shoots versus roots, clonal versus sexual reproduction, and survival of seedlings. These traits were explored in the chandelier plant (Kalanchoe delagoensis Eckl. & Zeyh.), which has invasive populations in several countries. Light and water tolerance and herbicide treatments were tested on plantlet survival. Plantlet survival in the most extreme cases (full sunlight and no watering) was close to 30%, whereas in less severe conditions (water and shaded), it was close to 100%. Stress conditions triggered the onset of plantlet production from the margin of leaves, which increased clonality. Biomass was allocated primarily to aboveground structures. Although all herbicides resulted in high plantlet mortality (>85%), only 2,4-D and glyphosate+2,4-D amine achieved the maximum recorded mortality a few days after the chemical application. The high tolerance of K. delagoensis plantlets to varying conditions shows that under stress, plantlet production is enhanced as survival of established individuals decreases. Biomass is primarily aboveground, which can potentially alter nitrogen and carbon in poor arid environments, and the proportion of the biomass assigned to belowground roots increased with an increase in sunlight received. Even though the chemical treatments 2,4-D and glyphosate+2,4-D amine have been shown to be the only effective treatments, the 2,4-D treatment may be the most viable (cost+quantity) to reduce the propagation of K. delagoensis. Plantlets have become the main reason for population persistence, partially due to the plant’s environmental tolerance and ability to reproduce asexually in short time periods. Susceptibility of plantlets to the two herbicides presents a means to adequately manage invasions of K. delagoensis in Mexico.

Little is known about the clustering patterns of lifestyle behaviours in adult populations. We explored clusters in multiple lifestyle behaviours including physical activity (PA), smoking, alcohol use and eating habits in a sample of adult population. A cross-sectional and multi-centre study was performed with six participating groups distributed throughout Spain. Participants (n 1327) were part of the Lifestyles and Endothelial Dysfunction (EVIDENT) study and were aged between 20 and 80 years. The lifestyle and cardiovascular risk (CVR) factors were analysed using a clustering method based on the HJ-biplot coordinates to understand the variables underlying these groupings. The following three clusters were identified. Cluster 1: unhealthy, 677 subjects (51 %), with a slight majority of men (58·7 %), who were more sedentary and smokers with higher consumption of whole-fat dairy products, bigger waist circumference as well as higher TAG levels, systolic blood pressure (SBP) and CVR. Cluster 2: healthy/PA, 265 subjects (20 %), including 24·0 % of males with high PA. Cluster 3: healthy/diet, including 29 % of the participants, with a higher consumption of olive oil, fish, fruits, nuts, vegetables and lower alcohol consumption. Using the unhealthy cluster as a reference, and after adjusting for age and sex, the multiple regression analysis showed that belonging to the healthy/PA cluster was associated with a lower waist circumference, body fat percentage, SBP and CVR. In summary, the three clusters were identified according to lifestyles. The ‘unhealthy’ cluster had the least favourable clinical parameters, the ‘healthy/PA’ cluster had good HDL-cholesterol levels and low SBP and the ‘healthy/diet’ cluster had lower LDL-cholesterol levels and clinical blood pressure.

The pelagic stingray Pteroplatytrygon violacea is known to be a frequent by-catch in longline fisheries worldwide. This study analysed the eco-geographic, technical and temporal parameters that affect pelagic stingray by-catch by the Spanish surface drifting longline fleet that operates in the Mediterranean Sea. Between 2000 and 2013, 3007 longline fishing operations were monitored. Over this period, we recorded 57 574 pelagic stingray by-catches by this fleet. Two gear types were involved in 96.05% of the pelagic stingray by-catch observed: traditional surface longliners targeting swordfish (LLHB) and surface drifting longliners targeting albacore (LLALB). We obtained two statistically significant explanatory models for the two types of gear. In both cases, two of the most important variables were fisheries being sited over the continental shelf and fishing during the summer season. The LLHB explanatory model included the following variables: number of hooks, latitude where setting started, distance between the ends of the longline, and the spring season. Regarding the LLHB, we found an association between the Capture per Unit Effort of pelagic stingray from favourable sets per year and the North Atlantic Oscillation in the previous year.

Arid regions are increasingly being anthropogenically altered. In the north-western Sahara, a growing road network facilitates the use of habitats adjacent to roads. In regions where livestock is the traditional and main economic resource, local people are currently building numerous water cisterns for watering livestock, leading to an increase in the extent of pasturing of domestic livestock. Cisterns may attract desert vertebrates and act as death traps for species with already sparse populations in these arid areas. This paper is the first to examine the impact of cisterns as lethal traps for amphibians and reptiles in the Sahara, using a survey of 823 cisterns in south-western Morocco to identify and quantify species affected. Four amphibians and 35 reptiles were trapped in cisterns, some of which were listed as threatened. At least 459 017 individual amphibians and reptiles were trapped annually within the study area. The low productivity and low population densities of terrestrial vertebrates in this arid region suggest cisterns have a substantial impact upon amphibian and reptile species. As cistern construction is increasing, management actions are required to mitigate this impact on the herpetological community.

The objective of this study was to verify the factor validity and structure of the “Irritation Scale” in a sample of 578 Spanish university students. At the same time, the study aimed to verify the criterion-related validity of the scale, analyzing the results obtained through correlation with other variables, such as general self-efficacy, self-regulation, depression, and certain personality dimensions. The results obtained through the Exploratory Structural Equation Modeling approach (ESEM) using Mplus confirmed the presence of two factors in the Irritation Scale, as observed in other international studies within a workplace setting. The significant correlations obtained between the Irritation Scale and the variables considered in the study confirmed the construct validity and verified that irritation is significantly and positively associated with depression and academic burnout, and is negatively associated with general self-efficacy and self-regulation.

Patella ferruginea is the most threatened macroinvertebrate in the western Mediterranean, where it is at serious risk of extinction. There is little information on the status of the various populations and most data were published more than 25 years ago. This study provides updated information on the global status of this species, and implications for management and conservation, and tests the hypothesis that population structure can be influenced by collection by people and by the type of substrate. Fifty-five localities were surveyed in Corsica, Sardinia, Tuscany and Sicily, on the islands of Egadi and Pantelleria, and on the Zembra archipelago and Tunisian coasts. The species is extinct on mainland Italy and Sicily but isolated individuals can be found on Egadi and Pantelleria. Populations on Corsica and Sardinia have declined dramatically during the last 25 years. The population in the Zembra archipelago is well preserved, although more widely on Tunisian coasts the species is highly threatened. The information provided here, combined with information from the literature, indicates a strong decline and/or extinction of many populations throughout the western Mediterranean and the presence of healthy populations only in some locations along the north African coast. The species exhibits an increase in density and mean size in areas free of human pressure but the type of substrate (natural or artificial) has no strong influence.