Objectives/Goals: Evaluate the effectiveness of ChatGPT as a complementary tool for addressing the mental health needs of Hispanic LGBTTQI+ young adult patients. We will also explore the experience of clinical healthcare providers with the integration of ChatGPT as a complementary tool in psychotherapy with Hispanic LGBTTQI+ young adults. Methods/Study Population: In this mixed-method study, we will create hypothetical clinical cases that reflect common mental health challenges experienced by LGBTTQI+ Hispanic population. These cases will be presented to three groups: 1) ChatGPT app, 2) clinical mental health care providers, and 3) clinical mental health care providers collaborating with ChatGPT. Each group will provide a diagnosis and a treatment plan based on the case information. A panel of experts will evaluate each plan using a standardized rubric to provide a score on clinical accuracy and on the ability to address specific needs of Hispanic LGBTTQI+ patients. Statistical analysis will be used to evaluate the differences in the scores of each domain and qualitative content analysis to evaluate the experience of clinical mental health care providers using ChatGPT. Results/Anticipated Results: The results will provide evidence of the effectiveness of ChatGPT as a supportive tool in mental health care. We anticipated that the combination of a clinical mental health care provider and ChatGPT to develop a diagnosis and treatment plan would produce better outcomes than either ChatGPT or the clinical mental health care provider working independently. We will also expect to find a positive attitude toward the integration of ChatGPT applications, viewing them as useful tools that complement traditional psychological interventions for Hispanic LGBTTQI+ young adults. The study will provide evidence of the effectiveness of ChatGPT to complementing clinical practice involving Hispanic LGBTTQI+ young adults. Those results in a preclinical phase are preconditions to a more applied intervention. Discussion/Significance of Impact: We aim to improve the quality of life for LGBTTQI+ Hispanics by developing innovative psychological treatments enhanced by AI apps. By developing innovative treatments, we are addressing and mitigating health disparities within the LGBTTQI+ Hispanic community in Puerto Rico and contributing to a broader effort of inclusivity and health equity.

Peru has the second-highest diversity of birds in the world, but little is known about the interactions between birds and plastic waste. To fill this knowledge gap, we searched the scientific literature, collected information from social networks such as Facebook and databases such as Macaulay Library and iNaturalist and solicited records through messaging with researchers and bird enthusiasts. We found 119 bird interactions with plastic debris involving 39 species from 20 families, with the red-legged cormorant Phalacrocorax gaimardi and the neotropical cormorant Phalacrocorax brasilianus being the most affected species. By type of interaction category, plastic waste in nests was the most abundant, followed by entanglement, capture and handling and ingestion. Ropes, nets and soft plastics such as bags were the most frequently reported types of waste. As our methodology has limitations, it is probable that other species that also interact with plastic waste have not been reported, so we recommend further study.

The hawksbill sea turtle Eretmochelys imbricata is categorized as Critically Endangered on the IUCN Red List and its population has declined by over 80% in the last century. The Eastern Pacific population is one of the most threatened hawksbill populations globally. Western Mexico is the northern distribution limit for hawksbill sea turtles in the Eastern Pacific and recent research indicates that the Mexican Pacific portion of the population is a separate management unit because of the restricted movements of these turtles. Here we use the most complete database of sighting records in the north-west Pacific of Mexico to identify sites where hawksbill turtles are present. We also develop a conservation index to determine the conservation status of hawksbill turtle sites. Our results demonstrate the importance of this region for juveniles and the relevance of rocky reefs and mangrove estuaries as habitats for hawksbill turtles. We identified 52 sites with records of hawksbill turtles. Most of these sites (71%) are not protected; however, sites with high conservation value included islands and coastal sites along the Baja California peninsula that are established as marine protected areas. Reefs and mangrove estuaries relevant for hawksbill turtles are probably also significant fish nursery areas that are important for local fishing communities, creating opportunities for conservation strategies that combine science, local engagement and policy to benefit both local fishing communities and hawksbill sea turtle conservation.

Molybdenum sulfide hydrotreating catalysts promoted with nickel over tridimensional mesoporous silica (KIT-6 post synthesis modified with alumina) were prepared with three different chelating agents. Citric acid and EDTA (ethylenediaminetetraacetic acid) were used as typical chelates and the new suggestion, polyacrylic acid as a polymeric agent. The catalysts were synthesized by the incipient wetness impregnation method, and two different activation methods were applied to determine the correlation between the chelating agent and activation conditions. The beneficial use of chelating agents was evaluated in their performance on HDS (hydrodesulfurization) of DBT (dibenzothiophene). To determine the properties of catalysts, nitrogen physisorption, X-ray diffraction, HRTEM (high-resolution transmission electron microscopy), and TGA (thermogravimetric analysis) were used. The beneficial effect of chelating Ni during impregnation to avoid NiSx formation and thus promoting NiMoS arrangement was clearly observed in the catalytic HDS performance, and the TGA analysis of Ni-chelate complexes also confirms this theory. The catalyst with the best performance in the HDS reaction of DBT was the synthesized with citric acid and a slow rate temperature sulfidation.

As a measure to manage the climate impact of aviation, significant enhancements to aviation technologies and operations are necessary. When assessing these enhancements and their respective impacts on the climate, it is important that we also quantify the associated uncertainties. This is important to support an effective decision and policymaking process. However, such quantification of uncertainty is challenging, especially in a complex system that comprises multiple interacting components. The uncertainty quantification task can quickly become computationally intractable and cumbersome for one individual or group to manage. Recognizing the challenge of quantifying uncertainty in multicomponent systems, we utilize a divide-and-conquer approach, inspired by the decomposition-based approaches used in multidisciplinary analysis and optimization. Specifically, we perform uncertainty analysis and global sensitivity analysis of our multicomponent aviation system in a decomposition-based manner. In this work, we demonstrate how to handle a high-dimensional multicomponent interface using sensitivity-based dimension reduction and a novel importance sampling method. Our results demonstrate that the decomposition-based uncertainty quantification approach can effectively quantify the uncertainty of a feed-forward multicomponent system for which the component models are housed in different locations and owned by different groups.

Analysis of dental calculus is increasingly important in archaeology, although the focus has hitherto been on dietary reconstruction. Non-edible material has, however, recently been extracted from the dental calculus of a Neanderthal population from the 49 000-year-old site of El Sidrón, Spain, in the form of fibre and chemical compounds that indicate conifer wood. Associated dental wear confirms that the teeth were being used for non-dietary activities. These results highlight the importance of dental calculus as a source of wider biographical information, and demonstrate the need to include associated data within research, in particular tooth wear, to maximise this valuable resource.

The photodegradation of polypropylene (PP)/ZnO composites at different concentrations was evaluated under solar simulated exposure of respective nanocomposite films. Nanocomposites were prepared by solid mixing using a cryogenic mill, and then films were prepared by compression molding. All films showed a good dispersion of ZnO nanoparticles without affect considerably the optical properties of the films. The films were exposed in a solar simulation chamber under three xenon arc lamps with a 340nm filter. Degradation of PP/ZnO nanocomposite films was monitored by formation of oxidative groups and changes on surface microstructure. FTIR results showed that oxidation groups in nanocomposites films increased by using cuasi-spherical ZnO nanoparticles.

We describe the investigation and control of a Klebsiella pneumoniae carbapenemase-producing K. pneumoniae outbreak in a 20-bed surgical intensive care unit during the period from January 1, 2009 through January 1, 2010. Nine patients were either colonized or infected with a monoclonal strain of K. pneumoniae. The implementation of a bundle of interventions on July 2009 successfully controlled the further horizontal spread of this organism.

This article reports mean scores on the Bayley Scales of Infant and Toddler Development (Version III) for 211 randomly selected healthy term (≥ 37 weeks gestation) Australian infants at one year of age. Mean scores were significantly different from standardised norms in all subscales except fine motor. Australian infants scored higher on cognitive and receptive language (p < .01) and lower on expressive language and gross motor (ps < .01) subscales. These findings raise questions regarding the validity of this test in the Australian population and suggest that the test be re-normed on Australian children for valid interpretation of scores in this cultural context.