Light, scanning, and transmission electron microscopy were employed to provide further data on the putative origins of the immunogenic secretory–excretory product (ESP) of Strongylus vulgaris (Looss 1900). The sharply delineated but superficial attachment to the equine caecum by the mouth leaves behind an oval area devoid of epithelial cells. Attachment does not extend deeply enough to reach the muscularis mucosa layer of the equine intestine. The progressive digestion of the ingested plug of tissue (epithelial cells, blood cells and mucous) was visualized. The coelomocytes, floating cells and membranous structures located in the pseudocoelom and intimately associated with the digestive, excretory and reproductive systems, and with the somatic muscles are described. The secretory–excretory system comprises two, ventrally-located, secretory–excretory glands connected to tubular elements. These glands synthesize granules of various sizes and densities that are delineated.

The study on the recognition of 35S-labelled somatic antigens of Gnathostoma spinigerum advanced third-stage larva (aL3) has revealed that the mAb GN6/24 immunoprecipitated 26- and 24-kDa proteins from the undigested and N-glycosidase F-digested larval extracts, respectively. The recognition of the deglycosylated form of the glycoprotein indicated that the mAb reacted with the peptide epitope on the 26-kDa protein. Human gnathostomiasis antiserum immunoprecipitated most of the N-glycosidase F-digested larval proteins including the deglycosylated 26-kDa protein.

Photoperiodic emergence patterns of the cercariae of two digeneans, Echinoparyphium recurvatum (Echinostomatidae) and Plagiorchissp. (Plagiorchiidae), were studied experimentally using a photoperiodic regime of 12 h light: 12 h dark at a constant 18°C. In single species infections of the first intermediate host snail Lymnaea peregra, the cercariae of E. recurvatum emerged exclusively during the light phase and those of Plagiorchis sp. emerged exclusively during the dark phase. In double infections, each species retained its own discrete photoperiodic emergence pattern. This result demonstrates the absence of interference between the mechanisms responsible for the photoperiodic emergence pattern of each species. A degree of interference between the two parasites in double infections of L. peregra was evident. The presence of E. recurvatum significantly reduced the number of Plagiorchis sp. cercariae emerging in a 24 h period and also delayed the mean peak emergence time of Plagiorchissp. cercariae by 2 h. This result is consistent with the well documented antagonistic effect that the redial stages of echinostomes are known to have against the sporocysts of other digeneans within the same host mollusc.

Phototactic responses of cercariae of the echinostome Echinoparyphium recurvatum (Digenea: Echinostomatidae) were investigated during phases of sub-maximal and maximal infectivity using a simple light/dark choice chamber. The phototactic responses of a total of 400 cercariae were examined in 20 replicate experiments during their sub-maxinal infectivity dispersal phase (0.5 h post-emergence from the first intermediate host Lymnaea peregra) and during their maximal infectivity phase (2.5 h post-emergence). Cercariae in the sub-maximal infectivity dispersal phase were found to be positively phototactic, significantly larger numbers of them occurring on the light side of a light/dark choice chamber P < 0.001. Cercariae in the maximal infectivity phase were found to be negatively phototactic, significantly larger numbers of them being found on the dark side of the choice chamber, P < 0.001. This report of ‘phototactic switching’ demonstrated by the cercariae of E. recurvatum from a positively phototactic dispersal phase to a negatively phototactic infection phase constitutes the first record of such behaviour in the cercaria of an echinostome.

The composition of the parasite fauna of the flounder, Platichthys flesus, retrieved from two locations in the tidal Thames is described in detail for the first time. The combined parasite species list of the flounders from Lots Road in the upper tideway and West Thurrock in the middle tideway consisted of one protozoan (Glugea stephani), one monogenean (Gyrodactylus sp.), four larval digeneans (Cryptocotyle concava, Timoniella imbutiforme, T. praeterita, and Labratrema minimus), five adult digeneans (Derogenes varicus, Lecithaster gibbosus, Podocotyle sp., Plagioporus varius, and Zoogonoides viviparus), one larval cestode (unidentified tetraphyllidean), one or possibly more larval nematodes (unidentified) plus five adult nematodes (Capillaria sp., Cucullanus heterochrous, C. minutus, Contracaecum sp. and Goezia sp.), two acanthocephalans (Pomphorhynchus laevis and Acanthocephalus anguillae), three copepods (Lepeophtheirus pectoralis, Acanthochondria sp. and Lernaeocera branchialis), and one mollusc (unidentified glochidia). The overall parasite community of flounders from Lots Road and West Thurrock were compared in terms of species richness and diversity. The parasite community in flounders from the former location in the upper tideway was found to be less diverse than that of its counterpart at West Thurrock in the middle estuary. The component community of Lots Road flounders was dominated by the acanthocephalan Pomphorhynchus laevis.

Hegel's Eurocentrism has become something of an elephant in the room, too obvious to merit discussion. The secondary literature on Hegel often concedes that many of Hegel's comments about the Orient are misguided and uninformed, and then declares that such remarks are of no consequence to his philosophy. However, recently, some scholars have contended that Hegel's view of freedom is fundamentally connected to his negative vision of the Orient and Africa. For example, Alison Stone asserts that Hegel's idea of freedom is premised on his Eurocentrism and colonialism. Drawing on Peter Sahota's essay on Hegel and Hinduism, she argues that Hegel's idea of freedom as liberation from nature tends to exclude non-Western conceptions of freedom. If Stone's contention is true, there would be no way to, as she puts it, save Hegel from himself. I argue that we can move some way towards rescuing Hegel by noting how scholars from modern Asia incorporated some of Hegel's ideas to struggle against Eurocentrism. This essay will read Hegel's comments on the Orient in light of his larger philosophy and then examine how Japanese scholars, specifically Okakura Tenshin (1863–1913) construct what could be called a Hegel-inspired defense of the concept of Asia or the Orient. I claim that if one understands the excesses of Western life to be linked to a vision of freedom based merely on self-interest and pleasure, when scholars and activists from Asia question Western culture, they actually follow Hegel's insights. Okakura, who studied Hegel in college, rethought Asia as overcoming the abstract individualist freedom of the West and replacing it a more holistic, and partially, Hegelian form of freedom.

Dynamic simulations of the cable-driven parallel robots (CDPRs) with cable models closer to reality can predict the motions of moving platforms more accurately than those with idealisations. Hence, the present work proposes an efficient and modular computational framework for this purpose. The primary focus is on the developments required in the context of CDPRs actuated by moving the exit points of cables while the lengths are held constant. Subsequently, the framework is extended to those cases where simultaneous changes in the lengths of cables are employed. Also, the effects due to the inertia, stiffness and damping properties of the cables undergoing 3D motions are included in their dynamic models. The efficient recursive forward dynamics algorithms from the prior works are utilised to minimise the computational effort. Finally, the efficacy of the proposed framework and the need for such an inclusive dynamic model are illustrated by applying it to different application scenarios using the spatial $4$-$4$ CDPR as an example.

The aim of this study was to determine if the plerocercoid larvae of Gymnorhynchus gigas, a common cestode of the ray’s bream (Brama raii), possess antigenic compounds potentially capable of provoking anaphylactic episodes. A murine experimental model, using BALB/c mice, was developed to study the humoral immune response induced by G. gigas extracts. A highly specific humoral immune response was detected and cross-reactions were not observed between parasite and host antigens. The presence of IgM and IgG3 levels suggest the presence of thymus-independent antigens in the parasitic extract. The IgG antibody class showed the highest levels, with the IgG1 the predominant subclass. These IgG1 levels are in accordance with the supposed presence of a type I allergic reaction after the ingestion of G. gigas plerocercoids parasitizing fish, as well as inducing anaphylaxia in fish. These results indicate that somatic products released from ingested larvae of G. gigas could induce the development of a Th2 response capable of causing allergic disorders.

The viability of metacercariae of Fasciola gigantica was tested by in vitro and in vivo methods. In vitro testing was based upon the motility of juvenile flukes within the inner cyst as examined under the light microscope. In vivo testing was undertaken through experimental infections of rabbits (two groups) and natural definitive hosts, lambs (one group). In the first group, out of six rabbits each given 25 metacercariae, worm establishment only took place in one rabbit with a single fluke recovery on 60 days post infection. In the second group of six rabbits each given 200 metacercariae, five were infected, with two or three flukes per host. All the lambs given 250 metacercariae became infected showing prevalences of 7.2–40% in comparison with rabbits in which low prevalences (0–4%) were recorded. The results indicated that even viable metacercariae which were already tested in vitro could not readily establish in rabbits. Such variability in worm establishment suggests that immunological and chemotherapeutic studies in rabbits infected with F. gigantica are likely to be unreliable.

Tetrahedrite-(Cu), Cu12Sb4S13, has been approved as a new mineral species (IMA2022–078) from the Bankov magnesite deposit near Košice, Slovak Republic where it occurs as anhedral grains, up to 0.4 mm across, associated with skinnerite, chalcostibite, famatinite, tetrahedrite-(Fe) and zoned aggregates of tennantite-(Cu) to tennantite-(Fe). Tetrahedrite-(Cu) is steel-grey, with a metallic lustre. Mohs hardness is ca. 3½–4 and calculated density is 5.029 g.cm–3. In reflected light, tetrahedrite-(Cu) is isotropic and grey with a bluish shade. Reflectance data for the four COM wavelengths in air are [λ (nm): R (%)]: 470: 31.1; 546: 30.1; 589: 29.9; and 650: 28.1. The empirical formula, based on electron-microprobe data (mean of 17 spot analyses), is Cu11.42Zn0.26Fe0.19(Sb4.06As0.08)Σ4.14S12.99. The ideal formula is Cu6(Cu4Cu2)Sb4S13, which requires (in wt.%) Cu 45.76, Sb 29.23 and S 25.01, total 100.00. Tetrahedrite-(Cu) is cubic, I$\bar{4}$3m, with unit-cell parameters a = 10.3296(15) Å, V = 1102.2(5) Å3 and Z = 2. Its crystal structure was refined by single-crystal X-ray diffraction data to a final R1 = 0.0347 on the basis of 261 unique reflections with Fo > 4σ(Fo) and 22 refined parameters. Tetrahedrite-(Cu) is isotypic with other tetrahedrite-group minerals. Previous findings of tetrahedrite-(Cu) are reported and some nomenclature issues, related to the Fe and Cu oxidation states, are discussed. At the Bankov deposit, tetrahedrite-(Cu) is related to hydrothermal, most probably Alpine, solutions strongly enriched in Cu, Sb and S.

Eggs of the tapeworm, Hymenolepis diminuta, and adults of the beetle, Tenebrio molitor, were incubated at high and low temperatures. The tapeworm's eggs survived at higher and lower temperatures and for longer periods of time than did adult beetles, indicating that the thermal tolerance of the eggs does not limit the parasite's distribution.

High-cardinality categorical features are pervasive in actuarial data (e.g., occupation in commercial property insurance). Standard categorical encoding methods like one-hot encoding are inadequate in these settings.

In this work, we present a novel Generalised Linear Mixed Model Neural Network (“GLMMNet”) approach to the modelling of high-cardinality categorical features. The GLMMNet integrates a generalised linear mixed model in a deep learning framework, offering the predictive power of neural networks and the transparency of random effects estimates, the latter of which cannot be obtained from the entity embedding models. Further, its flexibility to deal with any distribution in the exponential dispersion (ED) family makes it widely applicable to many actuarial contexts and beyond. In order to facilitate the application of GLMMNet to large datasets, we use variational inference to estimate its parameters—both traditional mean field and versions utilising textual information underlying the high-cardinality categorical features.

We illustrate and compare the GLMMNet against existing approaches in a range of simulation experiments as well as in a real-life insurance case study. A notable feature for both our simulation experiment and the real-life case study is a comparatively low signal-to-noise ratio, which is a feature common in actuarial applications. We find that the GLMMNet often outperforms or at least performs comparably with an entity-embedded neural network in these settings, while providing the additional benefit of transparency, which is particularly valuable in practical applications.

Importantly, while our model was motivated by actuarial applications, it can have wider applicability. The GLMMNet would suit any applications that involve high-cardinality categorical variables and where the response cannot be sufficiently modelled by a Gaussian distribution, especially where the inherent noisiness of the data is relatively high.

The potential influence of second intermediate host species on the infectivity of metacercarial cysts of Echinoparyphium recurvatum to the definitive host Anas platyrhynchos was examined experimentally. Echinoparyphium recurvatum metacercarial cysts were obtained from the following experimentally infected second intermediate hosts 14 days post expsoure to cercariae: Lymnaea peregra; Physa fontinalis; L. stagnalis; Planorbis planorbis; Biomphalaria glabrata; tadpoles of the amphibian Rana temporaria. Metacercarial cysts from each of these hosts were fed, in doses of 50 cysts per individual, to separate groups composed of between four and eight, 3-day-old A. platyrhynchos ducklings. All A. platyrhynchos were necropsied 15 days post-infection and the number, size, and reproductive status of E. recurvatum worms in the intestine was recorded. Analyses of variance on the number (transformed log (x + 1)) and size of worms revealed no significant differences in worms originating from metacercariae formed in the different second intermediate hosts (worm number P > 0.05, and worm size P > 0.05). All worms recovered were found to be gravid. It is therefore concluded that the species of second intermediate host utilized does not influence the infectivity of the metacercarial cyst of E. recurvatum, nor the subsequent establishment and reproductive status of the parasite in A. platyrhynchos.

Helminth parasites were studied in the wood mouse, Apodemus sylvaticus, in southern England in September of each of four successive years (1994–1997). Nine species of helminths were recorded: five nematodes (Heligmosomoides polygyrus, Syphacia stroma, Pelodera strongyloides, Trichuris muris, Capillaria murissylvatici), two cestodes (Microsomacanthus crenata, Taenia taeniaeformis) and two trematodes (Corrigia vitta, Brachylaemus recurvum). In total, 134 mice were examined and 91.8% carried at least one species of helminth. The majority of mice carried two to three species (60.5%) and the highest combination was six of the nine species recorded in the study. The patterns of between-year variations in the prevalence and abundance of infection were different for each of the six species for which sufficient quantitative data were available to enable statistical analysis. For H. polygyrus, the most important source of variation arose from between-year differences, host age and the interaction of these factors: abundance increased with host age but in 1995 the age pattern was markedly different from that in the remaining years. The abundance of C. vitta also varied significantly between years but additionally there was a strong independent age effect. For M. crenata, the year × age interaction was significant, indicating that abundance among different age cohorts varied from year to year but there was also a weak significant main effect of age arising from the youngest age cohort carrying no parasites and the oldest age cohort the heaviest infections. For P. strongyloides the only significant factor was between-year variation with 1995 being a year of exceptionally low prevalence and abundance of infection. No significant between-year variation was detected for S. stroma but there was a strong sex effect (males carrying heavier infections) and an age effect (older mice of both sexes carrying heavier infections). The abundance of Trichuris muris varied only in relation to host age, worm burdens growing in intensity with increasing age, but there was also a significant interaction between year and host sex with respect to prevalence. For the remaining three species, the prevalence of infections was too low (< 8.2%) to enable any meaningful interpretation. This analysis emphasizes the need for carefully controlled statistical procedures in aiding the interpretation and the prioritization of the factors affecting worm burdens in wild rodents.

Establishing aerothermal criteria for swept leading-edge hypersonic vehicle design is the predominant purpose of this work. This study is focused on two different configurations of vehicles based on the swept-back angle ($\varLambda$) viz. minimum drag ($\varLambda$Drag-min), and minimum heat transfer to vehicle ($\varLambda$HT-min). Maximum wall temperatures obtained from the simulation performed in ANSYS 2020 with the k-epsilon turbulence model are 1,013 and 970K for $\varLambda$Drag-min and $\varLambda$HT-min, respectively. These temperatures are used to obtain the corresponding thicknesses of thermal protection systems to maintain inner wall temperature at 323K. Further study is divided into two cases depending on the direction of thickness of thermal protection system with respect to vehicle body. For constant payload capacity, the direction of thickness is outside; whereas for constant overall volume case, direction of thickness is inside. For constant payload volume case, the percentage weight reduction of thermal protection system is 4.8%. For constant overall volume case, the percentage payload capacity increases with design at $\varLambda$HT-min by 4.04% in addition to thermal protection system weight reduction. The lift-induced drag on vehicles with design at $\varLambda$HT-min is significantly reduced for both cases, by 47.68% (for constant payload volume) and 45.27% (for constant overall volume).

In a sample of 61 free-living, postparasitic male Euchordodes nigromaculatus collected from a mountain stream in New Zealand, we found that only large males are found in areas of high current velocity. Thirty-five of the 61 males still contained gametes; these worms were found in wider, deeper, and slower-flowing parts of the stream relative to worms that had released their gametes. These results suggest that the physical characteristics of the immediate microhabitat of male worms can determine their probability of mating.

Inductively coupled plasma atomic emission spectrometry (ICP-AES) was used to study metallic ions in the intestinal mucosa of ICR mice infected with Echinostoma caproni and the mucosa of uninfected control mice. Infected mucosa (n = 9 with about 100 mg wet weight per sample) were examined at 2 weeks p.i. in mice that were infected with about 25 worms per host. Uninfected mucosa (n = 9 with about 100 mg wet weight per sample) were examined in the same time frame as the infected mucosa. Five metals were measured in the mucosa by ICP-AES analysis, as follows: calcium, potassium, magnesium, sodium and zinc. There were no significant differences (Student's t-test, P > 0.05) in the concentrations of calcium, potassium or zinc in infected versus uninfected mucosa. The concentration of sodium was significantly greater (P < 0.05) in the mucosa of infected versus uninfected mucosa, but the situation was reversed in regard to magnesium.

An attempt to compare and describe the differences in the electron density distribution between two phase structures of AlOOH has been made. High-resolution, high-pressure experiments with α-AlOOH diaspore were conducted using single-crystal synchrotron X-ray diffraction data. A multipole model of experimental electron density in the α-AlOOH single crystal was refined. Simultaneously, similar multipole refinement was conducted for another phase of diaspore (δ-AlOOH), this time based on a previously published data set. Both results were compared and supported by density functional theory (DFT) calculations. Although the results are affected by the limited quality of the data, it is clear that the phase transition caused significant changes in the shape and arrangement of the atomic basins.

Atomic basins are a much better tool to present subtle electron density distribution changes than traditional polyhedra. Straightforward comparison of datasets available in older scientific papers and current datasets is challenging because of differences in data quality and collection parameters. However, augmenting experimental data with computational results can help reveal important information in even incomplete datasets.

The ability of two isolates of the nematode-trapping fungus Duddingtonia flagrans to reduce the numbers of gastrointestinal nematode larvae on herbage was tested in three plot studies. Artificially prepared cow pats containing Ostertagia ostertagi eggs, with and without fungal spores, were deposited on pasture plots two or three times during the grazing season in 1995, 1996 and 1997. The herbage around each pat was sampled fortnightly over a period of 2 months and the number of infective larvae was recorded. At the end of the sampling period, the remainder of the faecal pats was collected to determine the wet weight, dry weight, and content of organic matter. The infective larvae remaining in the pats were extracted. Faecal cultures showed that both fungal isolates significantly reduced the number of infective larvae. Significantly fewer larvae were recovered from herbage surrounding fungus-treated pats compared with control pats in all three experiments, reflecting the ability of the fungus to destroy free-living larval stages in the faecal pat environment. After 8 weeks on pasture there were no differences between control and fungus-treated pats with respect to wet weight, dry weight, and organic matter content. This indicates that the degradation of faeces was not negatively affected by the presence of the fungus.