Dg2, a gene encoding a 34 kDa immunodominant antigen of Dirofilaria immitis was cloned and demonstrated to be specifically expressed in the larval stage. In this study, a newly constructed genomic DNA library was screened by hybridization with Dg2. One of the resulting positive clones was similar to Dg2 in the structure of its exonic regions but different in number, position, size and sequence of introns. This was designated DgK. Full-length cDNA was isolated using the rapid amplification of cDNA ends (RACE) method to study the transcript corresponding to DgK. Sequence analysis revealed that the mRNA corresponding to DgK is trans-spliced during post-transcriptional processing because the 5′ end of the amplified cDNA contains seven nucleotides of the nematode-spliced leader (SL) sequence.

Color variations from brown to yellow of synthesized goethite have been studied colorimetrically and spectroscopically. Goethite with various colors was synthesized at pH 13 and 40°C by varying the incubation time. Colorimetry revealed that the b* value (yellowish chroma) in L*a*b* color space was a quantitative indicator of color variations of the diluted samples. From UV-VIS-NIR spectra, the increase in the b* value was found to be caused by the increase in crystal field absorptions due to goethite formation around 500 nm. The b* value was a good indicator of the relative proportion of goethite in the precipitates including ferrihydrite. X-ray diffraction patterns and infrared spectra revealed that crystallization of goethite was associated with loss of water from the proto-ferrihydrite.

In a three-layer system with equal upper and lower layer thicknesses that are sufficiently thin and with the same density difference across each interface, breathers have been shown to exist using fully nonlinear governing equations. These breathers are well modelled by theoretical solutions of the mKdV equation, provided the interfaces between the layers do not cross a critical depth. The soliton-like characteristics of fully nonlinear breathers, in particular how two breathers interact, have yet to be explored. Using numerical simulations, this study addresses this shortcoming by studying fully nonlinear overtaking collisions of two breathers in a three-layer symmetric stratification. We apply the fully nonlinear and strongly dispersive FDI-3s internal wave equations, based on a variational principle, in a three-layer system. When the amplitude is small, the analytic breathers fit the wave shapes of the overtaking collision breathers. We find that the larger the upper and lower layer thicknesses are, provided they are below the critical thickness, the more the breathers behave like solitons. We show that an overtaking collision of two breathers is close to elastic.

In a three-layer system, weakly nonlinear theory predicts that breathers exist under certain conditions which, under the Boussinesq approximation, include symmetric stratifications in which the density jump across each interface is the same and the upper and lower layer thicknesses are equal and less than 9/26 of the total water depth. The existence and characteristics of fully nonlinear breathers in this symmetric stratification are poorly understood. Therefore, this study investigates fully nonlinear breathers in a three-layer symmetric stratification in order to clarify their characteristics by making direct comparisons between numerical simulation results and theoretical solutions. A normalization of the breather profiles is introduced using theoretical solutions of a breather and a new energy scale is proposed to evaluate their potential and kinetic energy. We apply fully nonlinear and strongly dispersive internal wave equations in a three-layer system using a variational principle. The computational results show that the larger the amplitude, the shorter the length of the envelope of breathers, which agrees with the theoretical solution. However, breathers based on the theoretical solutions cannot progress without deformation and decay due to the emission of short small-amplitude internal waves. Furthermore we demonstrate that the shedding of larger amplitude waves occurs, and the amplitude of the envelope decays more strongly when the density interface crosses the critical depth where the ratio of the upper layer thickness and the total water depth is 9/26 suggesting a limiting amplitude for fully nonlinear breathers.

An ultra-small tactile sensor with functions of signal processing and digital communication has been prototyped based on MEMS-CMOS integration technology. The designed analog-digital mixed signal ASIC allows many tactile sensors to connect each other on a common bus line, which drastically reduces the number of wire. The ASIC capacitively detects the deformation of a force sensor and sends digital data to the common bus line when the force exceeds a threshold. The digital data contain a physical ID of each sensor, 32-bit sensing data and 16-bit cyclic redundancy check (CRC) code. In this study, a novel wafer-level integration and packaging technology were developed, and a chip-size-packaged tactile sensor with a small footprint (2.5mm×2.5mm) and a low profile (0.27mm) was prototyped and tested. The sensor autonomously sends digital data like a tactile receptor of human.

Three-dimensional organic field-effect transistors with multiple sub-micrometer channels are developed to exhibit high current density and high switching speed. The sub-micrometer channels are arranged perpendicularly to substrates and are defined by the height of a multi-columnar structure fabricated without using electron-beam-lithography technique. For devices with dinaphtho[2,3-b:2',3'-f]thieno[3,2-b]thiophene, extremely high current density exceeding 10 A/cm2 and fast switching within 200 ns are realized with an on-off ratio of 105. The unprecedented performance is beyond general requirements to control organic light-emitting diodes, so that even more extensive applications to higher-speed active-matrices and display-driving circuits can be realized with organic semiconductors.

Summary

Background and objective: Thiopental has been reported to reduce sympathetic tone, however, it is not clear whether change in heart rate variability is associated with depth of anaesthesia. The purpose of the present study was to evaluate changes in heart rate variability at different depths of hypnosis during induction of anaesthesia with thiopental. Methods: We studied 17 ASA I patients scheduled for minor surgery. The depth of hypnosis was monitored by the BIS. Spectral analysis of heart rate variability using a maximum entropy method resulted in a characteristic power spectrum with two main regions, a high frequency and a low frequency. Haemodynamics, entropy, low frequency, high frequency and low frequency/high frequency were monitored in an awake state and after the induction of anaesthesia. Results: Heart rate increased in a BIS-dependent manner, whereas blood pressure showed no significant changes during the study period. High frequency, entropy and low frequency decreased with a reduction in the BIS value. Low frequency/high frequency showed no significant change during the study period. Conclusions: Induction of anaesthesia with thiopental increased heart rate and decreased high frequency, entropy and low frequency in a BIS-dependent manner, indicating that thiopental reduces cardiac parasympathetic tone depending on the depth of hypnosis.

We investigate fast simulation techniques for estimating the unreliability in large Markovian models of highly reliable systems for which analytical/numerical techniques are difficult to apply. We first show mathematically that for “small” time horizons, the relative simulation error, when using the importance sampling techniques of failure biasing and forcing, remains bounded as component failure rates tend to zero. This is in contrast to naive simulation where the relative error tends to infinity. For “large” time horizons where these techniques are not efficient, we use the approach of first bounding the unreliability in terms of regenerative-cycle-based measures and then estimating the regenerative-cycle-based measures using importance sampling; the latter can be done very efficiently. We first use bounds developed in the literature for the asymptotic distribution of the time to hitting a rare set in regenerative systems. However, these bounds are “close” to the unreliability only for a certain range of time horizons. We develop new bounds that make use of the special structure of the systems that we consider and are “close” to the unreliability for a much wider range of time horizons. These techniques extend to non-Markovian, highly reliable systems as long as the regenerative structure is preserved.

Using a known fact that a Galton–Watson branching process can be represented as an embedded random walk, together with a result of Heyde (1964), we first derive finite exponential moment results for the total number of descendants of an individual. We use this basic and simple result to prove analogous results for the population size at time t and the total number of descendants by time t in an age-dependent branching process. This has applications in justifying the interchange of expectation and derivative operators in simulation-based derivative estimation for generalized semi-Markov processes. Next, using the result of Heyde (1964), we show that, in a stable GI/GI/1 queue, the length of a busy period and the number of customers served in a busy period have finite exponential moments if and only if the service time does.

A process for the fabrication of textured Ba2CuO3 material with a microstructure similar to that of melt-processed YBa2Cu3Oy (Y123) superconductor is discussed. The Ba2CuO3 samples were melt processed with the intent to use them as textured precursors for processing of HgBa2CuOy superconductor. The microstructure formation of the Ba2CuO3 phase was studied by observing the samples being quenched from intermediate stages of a melt growth schedule. The microstructure of melt-processed Ba2CuO3 reveals randomly oriented large-sized grains, similar to that of melt textured Y123. Other important microstructural features observed were finely distributed properitectic BaO particles, the absence of the platelet gaps within the domains, and the presence of a different kind of twin structure. The conversion of melt textured Ba2CuO3 into superconducting HgBa2CuOy phase by a two-step process is discussed.

We conducted an epidemiological study of a Japanese encephalitis (JE) outbreak in the southwestern part of Nepal in 1997. A high density of JE infections was found and it was estimated that 27·9% the total population were infected with JE virus in the study area. The fatality rate was 13·2% and there was no difference in the fatality rate between males and females over 5 years old. However, the case fatality rate was 2·1 times higher in females than in males (14·6% vs. 6·9%) among children under 5 years of age. Fifty-three blood samples were collected from suspected JE cases during the epidemic period in 1998. Findings for JE specific IgM revealed that clinical diagnoses of JE were serologically confirmed in an average 78% (70–93%) of patients in three collaborating hospitals. These studies demonstrated that JE was highly prevalent in the area and clinical diagnoses were reliable. Effective preventive measures should be taken against this vaccine-preventable disease.

A single crystalline Bi2Sr2CaCu2O8+8 which has columnar defects in its inside are observed by Lorentz microscopy using the newly developed 1-MV field emission electron microscope at the first time. The superconducting vortices are observed with higher contrast than ever. Simultaneous observation of vortices and columnar defect is succeeded in real time.

Two types of shape and contrast features of superconducting vortices in a Lorentz micrograph were obtained by the newly developed 1-MV field-emission transmission electron microscope on a Bi2Sr2CaCu2O8+8(Bi-2212) thin specimen containing tilted columnar defects. The shape and contrast features could be consistently interpreted by the simulation that some vortices were pinned along tilted columnar defects and others were unpinned. The interesting property for temperature change of vortex core inside the material was also observed.

The classical regenerative method of simulation output analysis exploits the regenerative structure of a stochastic process to break up a path into independent and identically distributed cycles based on a single sequence of regeneration times. If a process is regenerative with respect to more than one sequence of regeneration times, the classical regenerative method does not exploit the additional structure, and the variance of the resulting estimator for certain performance measures (e.g., the time-average variance constant) can vary greatly, depending on the particular regeneration sequence chosen. In a previous article, we introduced an efficiency-improvement technique for regenerative simulation of processes having two sequences of regeneration times based on permuting regenerative cycles associated with the second sequence of regeneration points. In this article, we show how to exploit more than two regeneration sequences. In particular, for birth–death Markov chains, the regenerations associated with hitting times to each state can all be exploited. We present empirical results that show significant variance reductions in some cases, and the results seem to indicate that the permuted estimator for the time-average variance constant can have a variance that is independent of the primary regeneration sequence used to run the simulation.

The work function is one of the most fundamental properties of a metal surface. To clarify frictional electrification phenomena, the effects of frictional damage on the contact potential difference (CPD), which is defined by the difference between the work functions of two contacting surfaces, were investigated. Au(111) and Si(111) surfaces were scratched in an ultrahigh vacuum under a light load with the Si cantilever tip of an atomic force microscope. The contact potential difference between the scratched surface and the tip whose work function was known was measured using an ultrahigh vacuum scanning Kelvin probe force microscope (SKPM). Simultaneously, the noncontact atomic force microscope (NC-AFM) images were observed in situ. The CPD images showed clear changes between the areas with and without scratching, corresponding to the scratching track on the NC-AFM images.

Magnetocaloric effect of nanocomposites composed of iron-oxide or iron-nitride grains dispersed in a silver matrix was studied by calculating magnetic entropy change ΔS induced by a change in applied magnetic field H. These nanocomposites were synthesized by the inert gas condensation technique and nitridation by heat treatment in an ammonia gas stream. Average sizes of the iron-containing grains were 10-35 nm. Magnetic phases in the materials were Fe3O4 or γ -Fe2O3 for the oxide-composites and γ-Fe4N or ε -Fe3N for the nitride-composites. Values of the ΔS were obtained by applying a thermodynamic Maxwell's relation, (∂S / ∂H)T = (∂M / ∂T)H , to data set of magnetization M measured at various temperatures T. They clearly indicated significant enhancement due to the nanostructure as predicted.