Yeast species have several adaptations that enable them to survive in harsh environments. These adaptations include biofilm formation, where the secretion of extracellular polymeric substances can protect the cells from a hostile environment, or, under nutrient-limited conditions, pseudohyphal or hyphal growth, where the colony can send out long tendrils to explore the environment and seek nutrients. Recently, we observed a spiral colony morphology emerge in an isolate of the hyphae-forming yeast Magnusiomyces magnusii (M. magnusii) grown under laboratory conditions. We use an off-lattice agent-based model (ABM) that simulates colony development to investigate the hypothesis that bias in the angle between successive hyphal segments causes the spiral morphology. The model involves biologically motivated rules of hyphal extension, with key model parameters including the colony size at the onset of hyphal filaments, and the angle between the penultimate and the apical segments. Using one example of an experimentally grown colony, we use a sequential neural likelihood method to perform likelihood-free Bayesian inference to infer the model parameters. Our results indicate a mean angle between hyphal segments of ${2.3}^{\circ } [{1.1}^{\circ }, {3.6}^{\circ }]$ (95% credible interval). To confirm the model’s applicability to colony growth, we use biologically feasible parameter values to yield morphologies observed in M. magnusii experiments.

Maintenance procedures are critically important for preserving the structural integrity, maintaining the functionality and ensuring the operational safety of aircraft. Traditional inspection techniques used in aircraft are often costly, time-consuming and prone to human mistake. Today, the opportunities provided by digitalisation and automation in aircraft maintenance and inspection processes are paving the way for innovative approaches. In this context, the use of inspection systems supported by image processing technologies has the potential to bring about a significant transformation in aircraft maintenance. Visual inspection methods integrated with unmanned aerial vehicles (UAVs) enable the rapid, accurate and repeatable detection of defects such as corrosion and cracks on the external surfaces of aircraft. This study focuses on the automatic detection and classification of defects on the external surfaces of aircraft, based on tests and analyses carried out by artificial intelligence algorithms using high-resolution data. The model developed in this study was implemented in Python in the Google Colab environment and supported by AI algorithms trained on visual data. The main objective is to investigate the feasibility of UAV-based systems for aircraft visual inspection and to provide concrete evidence of their practical applicability. In this regard, the UAV platform selected for image acquisition is intended to comprehensively scan the target areas and capture images with sufficient resolution for processing by artificial intelligence algorithms. A review of the literature reveals that UAV- and AI-based integrated approaches have been explored in only a limited number of studies related to aircraft maintenance. In this context, the present study proposes a system that enables the rapid and accurate detection of structural defects such as corrosion and cracks on the external surfaces of aircraft.

Computer vision–based precision weed control has proven effective in reducing herbicide usage, lowering weed management costs, and enhancing sustainability in modern agriculture. However, developing deep learning models remains challenging due to the effort required for weed dataset annotation and the difficulty of identifying weeds at different stages and densities in complex field conditions. To address these challenges, this study introduces an indirect weed detection method that combines deep learning and image processing techniques. The proposed approach first employs an object detection network to identify and label crops within the images. Subsequently, image processing techniques are applied to segment the remaining green pixels, thereby enabling indirect detection of weeds. Furthermore, a novel detection network—CD-YOLOv10n (You Only Look Once version 10 nano)—was developed based on the YOLOv10 framework to optimize computational efficiency. Redesigning the backbone (C2f-DBB) and integrating an optimized upsampling module (DySample) permitted the network to achieve higher detection accuracy while maintaining a lightweight structure. Specifically, the model achieved a mean average precision (mAP50) of 98.1%, which is a 1.4% percentage-point increase compared with the YOLOv10n baseline, a relevant improvement given the already strong baseline performance. At the same time, compared with YOLOv10n, its GFLOPs (giga floating-point operations per second) were reduced by 22.62%, and the number of parameters decreased by 15.87%. These innovations make CD-YOLOv10n highly suitable for deployment on resource-constrained platforms.

Good air quality is a critical determinant of public health, influencing life expectancy, respiratory health, work productivity, and the prevention of chronic diseases. This study presents a novel approach to classifying the Air Quality Index (AQI) using deep learning techniques, specifically convolutional neural networks (CNNs). We collected and curated a dataset comprising 11,000 digital images from three distinct regions in Indonesia—Jakarta, Malang, and Semarang—ensuring uniformity through standardized acquisition settings. The images were categorized into four air quality classes: good, moderate, unhealthy for sensitive groups, and unhealthy. We designed and implemented a CNN architecture optimized for AQI classification. The model achieved an impressive accuracy of 99.81% using K-fold cross-validation. In addition, the model’s interpretative capabilities were examined using techniques such as Grad-CAM, providing valuable insights into how the CNN identifies and classifies air quality conditions based on image features. These findings underscore the effectiveness of CNNs for AQI classification and highlight the potential for future work to incorporate a more diverse set of digital images captured from various perspectives to enhance dataset complexity and model robustness. The dataset is publicly accessible at https://doi.org/10.5281/zenodo.15727522.

This research paper proposes a simple image processing technique for automatic lameness detection in dairy cows under farm conditions. Seventy-five cows were selected from a dairy farm and visually assessed for a reference/real lameness score (RLS) as they left the milking parlor, while simultaneously being video-captured. The method employed a designated walking path and video recordings processed through image analysis to derive a new computerized automatic lameness score (ALDS) based on calculated factors from back arch posture. The proposed automatic lameness detection system was calibrated using 12 cows, and the remaining 63 were used to evaluate the diagnostic characteristics of the ALDS. The agreement and correlation between ALDS and RLS were investigated. ALDS demonstrated high diagnostic accuracy with 100% sensitivity and specificity and was found to be 100% accurate with a perfect agreement (ρc = 1) and strong correlation (r = 1, P < 0.001) for lameness detection in binary scores (lame/non-lame). Moreover, the ALDS had a strong agreement (ρc = 0.885) and was highly correlated (r = 0.840; 0.796–1.000 95% confidence interval, P < 0.001) with RLS in ordinal scores (lameness severity; LS1 to LS5). Our findings suggest that the proposed method has the potential to compete with vision-based lameness detection methods in dairy cows in farm conditions.

Bees play a significant role in the health of terrestrial ecosystems. The decline of bee populations due to colony collapse disorder around the world constitutes a severe ecological danger. Maintaining high yield of honey and understanding of bee behaviour necessitate constant attention to the hives. Research initiatives have been taken to establish monitoring programs to study the behaviour of bees in accessing their habitat. Monitoring the sanitation and development of bee brood allows for preventative measures to be taken against mite infections and an overall improvement in the brood's health. This study proposed a precision beekeeping method that aims to reduce bee colony mortality and improve conventional apiculture through the use of technological tools to gather, analyse, and understand bee colony characteristics. This research presents the application of advanced digital image processing with computer vision techniques for the visual identification and analysis of bee brood at various developing stages. The beehive images are first preprocessed to enhance the important features of object. Further, object is segmented and classified using computer vision techniques. The research is carried out with the images containing variety of immature brood stages. The suggested method and existing methods are tested and compared to evaluate efficiency of proposed methodology.

In vivo fluorescence microscopy is a powerful tool to image the beating heart in its early development stages. A high acquisition frame rate is necessary to study its fast contractions, but the limited fluorescence intensity requires sensitive cameras that are often too slow. Moreover, the problem is even more complex when imaging distinct tissues in the same sample using different fluorophores. We present Paired Alternating AcQuisitions, a method to image cyclic processes in multiple channels, which requires only a single (possibly slow) camera. We generate variable temporal illumination patterns in each frame, alternating between channel-specific illuminations (fluorescence) in odd frames and a motion-encoding brightfield pattern as a common reference in even frames. Starting from the image pairs, we find the position of each reference frame in the cardiac cycle through a combination of image-based sorting and regularized curve fitting. Thanks to these estimated reference positions, we assemble multichannel videos whose frame rate is virtually increased. We characterize our method on synthetic and experimental images collected in zebrafish embryos, showing quantitative and visual improvements in the reconstructed videos over existing nongated sorting-based alternatives. Using a 15 Hz camera, we showcase a reconstructed video containing two fluorescence channels at 100 fps.

The Australian SKA Pathfinder (ASKAP) is being used to undertake a campaign to rapidly survey the sky in three frequency bands across its operational spectral range. The first pass of the Rapid ASKAP Continuum Survey (RACS) at 887.5 MHz in the low band has already been completed, with images, visibility datasets, and catalogues made available to the wider astronomical community through the CSIRO ASKAP Science Data Archive (CASDA). This work presents details of the second observing pass in the mid band at 1367.5 MHz, RACS-mid, and associated data release comprising images and visibility datasets covering the whole sky south of $\delta_{\text{J2000}}=+49^\circ$. This data release incorporates selective peeling to reduce artefacts around bright sources, as well as accurately modelled primary beam responses. The Stokes I images reach a median noise of 198 $\mu$Jy PSF$^{-1}$ with a declination-dependent angular resolution of 8.1–47.5 arcsec that fills a niche in the existing ecosystem of large-area astronomical surveys. We also supply Stokes V images after application of a widefield leakage correction, with a median noise of 165 $\mu$Jy PSF$^{-1}$. We find the residual leakage of Stokes I into V to be $\lesssim 0.9$–$2.4$% over the survey. This initial RACS-mid data release will be complemented by a future release comprising catalogues of the survey region. As with other RACS data releases, data products from this release will be made available through CASDA.

Manufacturing process (MP) selection systems require a large amount of labelled data, typically not provided as design outputs. This issue is made more severe with the continuous development of Additive Manufacturing systems, which can be increasingly used to substitute traditional manufacturing technologies. The objective of this paper is to investigate the application of image processing for classifying MPs in an unsupervised approach. To this scope, k-means and hierarchical clustering algorithms are applied to an unlabelled image dataset. The input dataset is constructed from freely accessible web databases and consists of twenty randomly selected CAD models and corresponding images of machine elements: 35% additively manufactured parts and 65% manufactured with traditional manufacturing technologies. The input images are pre-processed to have the same colour and size. The k-means and hierarchical clustering algorithms reported 65% and 60% accuracy, respectively. The algorithms show comparable performance, however, the k-means algorithm failed to predict the correct subdivisions. The research shows promising potential for MP classification and image processing applications.

This paper presents a low-cost, accurate indoor positioning system that integrates image acquisition and processing and data-driven modeling algorithms for robotics research and education. Multiple overhead cameras are used to obtain normalized image coordinates of ArUco markers, and a new procedure is developed to convert them to the camera coordinate frame. Various data-driven models are proposed to establish a mapping relationship between the camera and the world coordinates. One hundred fifty data pairs in the camera and world coordinates are generated by measuring the ArUco marker at different locations and then used to train and test the data-driven models. With the model, the world coordinate values of the ArUco marker and its robot carrier can be determined in real time. Through comparison, it is found that a straightforward polynomial regression outperforms the other methods and achieves a positioning accuracy of about 1.5 cm. Experiments are also carried out to evaluate its feasibility for use in robot control. The developed system (both hardware and algorithms) is shared as an open source and is anticipated to contribute to robotic studies and education in resource-limited environments and underdeveloped regions.

Electron cryo-tomography is an imaging technique for probing 3D structures with at the nanometer scale. This technique has been used extensively in the biomedical field to study the complex structures of proteins and other macromolecules. With the advancement in technology, microscopes are currently capable of producing images amounting to terabytes of data per day, posing great challenges for scientists as the speed of processing of the images cannot keep up with the ever-higher throughput of the microscopes. Therefore, automation is an essential and natural pathway on which image processing—from individual micrographs to full tomograms—is developing. In this paper, we present Ot2Rec, an open-source pipelining tool which aims to enable scientists to build their own processing workflows in a flexible and automatic manner. The basic building blocks of Ot2Rec are plugins which follow a unified application programming interface structure, making it simple for scientists to contribute to Ot2Rec by adding features which are not already available. In this paper, we also present three case studies of image processing using Ot2Rec, through which we demonstrate the speedup of using a semi-automatic workflow over a manual one, the possibility of writing and using custom (prototype) plugins, and the flexibility of Ot2Rec which enables the mix-and-match of plugins. We also demonstrate, in the Supplementary Material, a built-in reporting feature in Ot2Rec which aggregates the metadata from all process being run, and output them in the Jupyter Notebook and/or HTML formats for quick review of image processing quality. Ot2Rec can be found at https://github.com/rosalindfranklininstitute/ot2rec.

An emergent volume electron microscopy technique called cryogenic serial plasma focused ion beam milling scanning electron microscopy (pFIB/SEM) can decipher complex biological structures by building a three-dimensional picture of biological samples at mesoscale resolution. This is achieved by collecting consecutive SEM images after successive rounds of FIB milling that expose a new surface after each milling step. Due to instrumental limitations, some image processing is necessary before 3D visualization and analysis of the data is possible. SEM images are affected by noise, drift, and charging effects, that can make precise 3D reconstruction of biological features difficult. This article presents Okapi-EM, an open-source napari plugin developed to process and analyze cryogenic serial pFIB/SEM images. Okapi-EM enables automated image registration of slices, evaluation of image quality metrics specific to pFIB-SEM imaging, and mitigation of charging artifacts. Implementation of Okapi-EM within the napari framework ensures that the tools are both user- and developer-friendly, through provision of a graphical user interface and access to Python programming.

Galaxy-galaxy strong lensing in galaxy clusters is a unique tool for studying the subhalo mass distribution, as well as for testing predictions from cosmological simulations. We describe a novel method that simulates realistic lensed features embedded inside the complexity of observed data by exploiting high-precision cluster lens models. Such methodology is used to build a large dataset with which Convolutional Neural Networks have been trained to identify strong lensing events in galaxy clusters. In particular, we inject lensed sources around cluster members using the images acquired by the Hubble Space Telescope. The resulting simulated mock data preserve the complexity of observation by taking into account all the physical components that could affect the morphology and the luminosity of the lensing events. The trained networks achieve a purity-completeness level of ∼ 91% in detecting such events. The methodology presented can be extended to other data-intensive surveys carried out with the next-generation facilities.

Upcoming large-scale surveys like LSST are expected to uncover approximately 105 strong gravitational lenses within massive datasets. Traditional manual techniques are too time-consuming and impractical for such volumes of data. Consequently, machine learning methods have emerged as an alternative. In our prior work (Thuruthipilly et al. 2022), we introduced a self-attention-based machine learning model (transformers) for detecting strong gravitational lenses in simulated data from the Bologna Lens Challenge. These models offer advantages over simpler convolutional neural networks (CNNs) and competitive performance compared to state-of-the-art CNN models. We applied this model to the datasets from Bologna Lens Challenge 1 and 2 and simulated data on Euclid.