The present study describes a new Ditylenchoides species, isolated from Meknès, Morocco, during nematode surveys conducted to investigate the biodiversity of plant-parasitic nematodes in Mediterranean olive groves and adjacent patches of natural vegetation. Application of integrative taxonomical approaches clearly verified that it is a new species designated herein as Ditylenchoides morocciensis sp. nov., also representing the first report of the genus in Morocco. The new species is parthenogenetic, characterised by a short body 460 (373–528 μm); stylet delicate, relatively short, 8.7 (8.0–9.0) μm long with rounded basal knobs; six lines in the lateral fields; median bulb of pharynx oval, muscular and valvate; secretory-excretory pore located at the level of basal pharyngeal bulb region; vulva located at 79.9 (76.9–81.3) % of body length; relatively long post-vulval uterine sac 29.1 (20.0–39.0) μm; and a subcylindrical tail 24.4 (22.0–28.0) μm long, with a bluntly rounded tip. The results of molecular analysis of D2-D3 28S rRNA, ITS rRNA, partial 18S rRNA, and cytochrome oxidase c subunit 1 (COI) gene sequences support for the new species status and clearly separated D. morocciensis sp. nov. from all other species within Ditylenchoides. Phylogenetic analyses of ribosomal markers (D2-D3 28S rRNA and partial 18S rRNA) of this study confirms that Ditylenchoides is a monophyletic genus, clearly separated from other genera within Anguinoidea.

The reproductive efficiency of dairy cows decreases significantly in hot climates. Exposure to heat stress causes damage to different stages of the reproductive cycle including a decrease in the quality of oocytes. Antioxidant supplementation has been introduced as one of the main approaches to alleviate the effects of free radical damage associated with heat stress. Gamma-oryzanol (ORY), a component of rice bran oil, is introduced as a novel antioxidant. As a supplement of culture media for maturation, the effect of ORY on the development of heat-shocked bovine cumulus–oocyte complexes was evaluated in this study. At the end of maturation in vitro using the heat-shock model, a higher proportion of metaphase II oocytes (0.78 ± 0.03 vs 0.42 ± 0.03) and lower metaphase I and germinal vesicle breakdown (0.10 ± 0.02 vs 0.38 ± 0.03) were recorded for the treated group (N = 205) in comparison with the control (N = 203) (P < 0.05). Moreover, the treatment exerted upregulation of NRF2, SOD, CAT and GPX transcripts in matured oocytes and GPX in CCs, along with a considerable increase in the cleavage (0.52 ± 0.04 vs 0.33 ± 0.03) and total blastocyst rates (0.30 ± 0.03 vs 0.14 ± 0.02) (P < 0.05). These results showed that ORY increased the mRNA expression of the transcripts associated with antioxidant enzymes and enhanced the developmental potential of heat-shocked bovine oocytes and warranted further studies to explore this antioxidant’s influence on improving dairy cattle’s reproductive efficiency during heat stress.

Ostracods from the late Mississippian–early Pennsylvanian (late Serpukhovian–Bashkirian) from the Calingasta–Uspallata Basin, Precordillera Argentina, are studied for the first time. The analyzed successions (Hoyada Verde, El Paso, Leoncito, and Yalguaraz formations) document the most widespread glacial event in southwestern Gondwana during the Late Paleozoic Ice Age. One new species, Aechmina cuyanensis new species, is defined, and seven species are described, two of which are new records for South America. The distribution of Carboniferous ostracods in the Argentine basins (Calingasta–Uspallata, Río Blanco, and Tepuel–Genoa) is discussed, highlighting the interesting record of this fauna in levels between or below diamictites and its absence in the mudstone interval with no evidence of glaciation. The association is characterized by ornamented palaeocopids, one binodicopid, and some metacopid species corresponding to the Assemblage III of the Eifelian Mega-Assemblage, indicating a slightly hypoxic and very calm environment between the fair-weather wave base and the storm wave base.

UUID: https://zoobank.org/68fda8dc-be98-4a03-b369-a696b7d7ad99

This study aimed to explore the associations between weight gain during infancy with pre-pregnant body mass index (BMI) later in life, focusing on risks of being overweight or underweight. A retrospective cohort study was conducted using data from women (n = 1082) who visited the National Center for Child Health and Development between 2017 and 2021. The participants provided their Maternal and Child Health Handbook, which included records of their own birthweight and weight gain from birth to 1, 3, and 6 months. The infant weight gain was divided into quintiles. Multivariable logistic regression was used to assess the association of weight gain during infancy with pre-pregnant underweight (BMI < 18.5) and overweight (BMI ≥ 25) later in life, adjusting for potential confounders. The current study found that the largest weight gain category (5230–7700 g) by 6 months was associated with a decreased risk of “pre-pregnant underweight,” compared to the third weight gain category (4355–4730 g) by 6 months (OR, 0.40; 95% CI, 0.22–0.73). In contrast, no significant association was observed between weight gain category in infancy and being overweight in adulthood. In conclusion, greater weight gain during the first 6 months of life was associated with a reduced risk of “adult underweight,” without increasing the risk of being overweight.

We describe the new lichen species Lecanora austrocalcicola from a limestone outcrop in Brazil, characterized by a verrucose thallus and small apothecia with pale discs with relatively prominent margins that are UV+ yellow, and by the presence of lichexanthone on the apothecium margin. It resembles some species of Myriolecis and Polyozosia but can be distinguished with phylogenetic analyses.

Disease resistance breeding of wheat using molecular markers aims to efficiently identify and incorporate key resistance genes against stripe rust into elite wheat cultivars. This approach enhances the precision and speed of breeding programs to develop durable, resistant wheat varieties capable of withstanding Puccinia striiformis f. sp. tritici (Pst) infections, ultimately contributing to sustainable wheat production and global food security. A total of 174 bread wheat genotypes, comprising 94 Turkish cultivars and 80 exotic landraces obtained from the USDA, were screened using gene-specific markers for the stripe rust resistance genes Yr15 and Yr5. The Yr15 resistance gene was detected in 31 genotypes, including 22 Turkish cultivars and nine USDA landraces. The Yr5 resistance gene was not found in any of the tested germplasm, but the susceptibility gene was identified in 77 genotypes. Turkish cultivars showed higher susceptibility to stripe rust, with 46 out of 94 genotypes vulnerable, compared to 25 out of 80 USDA genotypes. These findings highlight the urgent need to integrate Yr15 and other resistance genes into wheat breeding programs to enhance crop resilience against evolving Pst isolates, thereby ensuring sustainable wheat production and contributing to global food security.

Aneuploidy in oocytes is a leading cause of implantation failure, miscarriage and congenital disorders. During meiosis, proper timing of chromosome segregation is regulated by the spindle assembly checkpoint (SAC) and the anaphase-promoting complex/cyclosome (APC/C). However, how pharmacological manipulation of these regulatory pathways affects aneuploidy remains incompletely understood. In this study, we investigated whether SAC inhibition by reversine induces aneuploidy in mouse oocytes and whether partial inhibition of APC/C by proTAME can alleviate these errors. Germinal vesicle (GV) oocytes were matured in vitro in the presence of various concentrations of reversine. To optimize the timing of treatment, oocytes were exposed to reversine for 0, 3, 5 or 7 h, followed by culture with or without proTAME. A proTAME-only group (2.5 nM) was also included. Chromosome spreads were analyzed at the metaphase II (MII) stage to determine aneuploidy rates. Reversine (5 nM) yielded an MII maturation rate of 80.5% but induced a high aneuploidy rate of 77.0%. Sequential treatment with 2.5 nM proTAME significantly reduced aneuploidy to 33.3%. In contrast, proTAME alone led to 79.0% aneuploidy, suggesting its effect is contingent upon prior SAC disruption. These results indicate that reversine compromises chromosomal integrity, while appropriately timed, low-dose proTAME can partially rescue segregation errors. Our findings underscore the potential of pharmacologically regulating APC/C activity to reduce aneuploidy and enhance oocyte quality, offering new avenues for improving outcomes in assisted reproductive technologies.

This research communication hypothesizes that superstimulation with follicle-stimulating hormone (FSH) would not impact behavior and performance of Holstein cows. The objectives were to investigate the effect of FSH superstimulation on follicular dynamics, animal behaviour, body surface temperature and milk yield of Holstein cows. Cows were blocked by parity and body condition score (BCS), and within a block, they were assigned randomly to receive either normal saline (CON = 7) or 500 IU of FSH (n = 8). The estrous cycle of cows was synchronized by using two injections of prostaglandin F2-alpha (PG), 11 days apart. The dominant follicle was ablated at the time of the second PG injection, and an intra-vaginal controlled internal drug release (CIDR) was inserted (day 0). Two days later, FSH treatment was initiated and continued for 3 days in six equal doses of 83.33 IU after 12 h. Follicles were counted and their sizes were measured from day-0 to day-5. Behavior, including activity and feeding time, was recorded using SmartTag Neck from day 0 to 6. The surface temperatures of the eye, shoulder, flank, and vulva were measured by using infrared thermal imaging every 12 h from day-2 to day-5. Milk was recorded from day-0 to day-6. Mixed effects models were used to analyse the data using SAS statistical software. The number of small and medium follicles did not differ between treatments. However, FSH-treated cows had a more (P = 0.01) large and total follicles compared with CON cows. FSH treatment did not affect activity, feeding time, body surface temperature, or milk yield. In conclusion, FSH superstimulation increased the number of large follicles but did not influence behaviour, body surface temperature, or performance in dairy cows.

Pollen morphology, viability and in vitro germination are vital parameters for taxonomic classification, hybridization, artificial pollination and improving seed set in crop breeding. In the present study, we investigated the pollen morphological characteristics of four marigold genotypes using scanning electron microscopy, assessed pollen viability using Alexander’s stain and acetocarmine and evaluated in vitro germination under a light microscope using media supplemented with varying concentrations of sucrose and polyethylene glycol (PEG). Pollen grains were medium-sized (25–50 µm) and exhibited suboblate to oblate-spheroidal shapes, with a polar/equatorial ratio ranging from 0.84 to 0.88. All genotypes displayed tricolporate pollen grains with three colpi and endoaperture, and their aperture lengths were between 3.88 and 4.90 µm. Significant genotypic differences were observed in pollen viability, with the highest values recorded in IIHRMY 1-4 (96.66 ± 0.34%) using acetocarmine and in IIHRMY 2-1 (96.42 ± 0.51%) using Alexander’s stain. The optimal germination medium contained 15% sucrose and 15% PEG, with IIHRMY 1-4 exhibiting the highest pollen germination rate (54.09 ± 1.15%), followed by IIHRMY 2-1 (47.05 ± 1.27%). These findings offer valuable insights for marigold breeding programmes, particularly in selecting genotypes for efficient cross-pollination and hybrid development.

A Palaeolithic diet is an efficacious dietary approach for glycaemic control in type 2 diabetes. Causal mechanisms are body weight loss and glucometabolic effects from differences in included food groups, macronutrient composition, fibre content, and glycaemic load. The aim was to test the hypothesis that characteristic food group differences between a Palaeolithic and a diabetes diet would cause an effect on glycaemic control when weight was kept stable and diets were matched for macronutrient composition, fibre content and glycaemic load. Adult participants with type 2 diabetes and increased waist circumference were instructed to follow two diets, with or without the food groups cereal grain, dairy products, and legumes, during two periods of 4 weeks separated by a 6-week washout period in a random-order crossover design. The Palaeolithic diet included fruit, vegetables, tubers, fish, shellfish, lean meat, nuts, eggs and olive oil, and excluded cereal grains, dairy products and legumes. The diabetes diet included fruit, vegetables, fish, shellfish, lean meat, nuts, eggs, olive oil, and substantial amounts of whole grains, low-fat dairy products and legumes. Dietary energy content was adjusted throughout the study to maintain stable body weight. There were no differences between diets on HbA1c or fructosamine among the 14 participants. Body weight was kept stable, and the two diets were successfully matched for macronutrient composition and glycaemic load but not for fibre content. Characteristic food group differences and the accompanying differences in fibre content between a Palaeolithic and a diabetes diet do not cause an effect on glycaemic control.

Anaesthesia methods play a crucial role in ensuring the integrity of the animal during experimental studies. This study investigates the impact of two anaesthesia methods, CO₂ and cold treatment, on an insect antennal response to synthetic alarm pheromone compounds. Adult worker hornets were anesthetised, and their antennae excised and tested using an electroantennography set-up with controlled stimulation of alarm pheromone components. Results showed that CO₂-anesthetised hornets exhibited robust antennal responses, while cold-anesthetised individuals displayed none. This result suggests that freezing may impair the functionality of olfactory receptors. In contrast, CO₂ anaesthesia preserves receptor integrity, offering reliable and interpretable results. This study highlights the importance of selecting appropriate anaesthesia techniques to avoid artefacts in insect sensory physiology research and underscores the ecological relevance of studying Vespa velutina nigrithorax alarm signalling.

The study of the ground surface temperature (GST) regimes from 2007 to 2021 at different stations on Livingston and Deception islands, South Shetland Islands, in the north-western sector of the Antarctic Peninsula (AP), shows that soils undergo similar cooling in early winter before a shallow snow mantle covers the sites. All monitoring sites along the study period go through seasonal phases of cooling, attenuation, insulation, fusion and zero curtain during winter, although thermal equilibrium is only reached at some stations located at lower elevations on Livingston Island. GST evolution at these stations and the duration of snow periods show oscillations, with turning points in the years 2014 and 2015, when temperatures were at their minimum and snow durations were at their maximum, in agreement with the cooling period occurring in the north-western AP in the early twenty-first century. The thermal regime is mainly controlled by snow cover and its onset and offset dates based only on descriptive patterns, not on statistical testing, more than by altitudinal, topographical, geological or geomorphological factors.

A set of 710 accessions of global durum wheat panel was obtained from the International Centre for Agricultural Research in Dryland Areas (ICARDA) and evaluated for variability in 32 agro-morphological traits at the ICAR-Indian Institute of Wheat and Barley Research, Karnal, India, during 2021–22 and 2022–23. The accessions were grouped based on the type of breeding material and geographical origin, and the Shannon–Wiener diversity index (H′) was calculated for each trait as well as each group. The H′ values ranged from 0.02 (grain crease) to 1.27 (wax of ear) with an overall mean of 0.63, when considering the entire set as a single group. Landraces exhibited higher variability (H′ = 0.64) as compared to released cultivars and evolutionary pre-breeding population lines (H′ = 0.55). Furthermore, accessions originating from West Asia exhibited the highest variability (H′ = 0.63). Traits such as coleoptile colour, plant growth habit, auricle colour and pubescence, flag leaf attitude, ear shape, presence or absence of awns, awns colour and attitude, glumes shape and pubescence, beak length, peduncle attitude, grain phenol colour, grain germ width and brush hair length were found suitable for distinguishing the present durum accessions due to their higher frequency distribution and Shannon–Wiener diversity index values. A core set of 13 accessions was developed using the programme Power Core. These accessions represent the overall variability of the entire set and can therefore serve as a potential source of new genes/alleles for the genetic enhancement of durum wheat. This durum panel may also serve as a valuable reference collection for global researchers in the characterization of durum wheat accessions.

Genebanks worldwide play a crucial role in the long-term conservation and accessibility of wide diversity of plant genetic resources (PGR). These resources are essential for addressing current and future challenges related to climate change and biodiversity loss, thereby contributing to agricultural production and sustainability. Enhancing the efficiency of genebanks requires implementing a quality management system that ensures data transparency, as well as the documentation and standardization of processes. This study proposes a set of 10 mandatory and 38 optional genebank metrics specifically designed for collections of orthodox seeds. These metrics define a set of basic parameters that are easy to calculate and collectively serve as a valuable tool for improving genebank management, enhancing performance, fostering transparency and promoting collaborations among institutions. Fourteen institutions engaged in the long-term conservation of PGR were consulted in the development of the proposed metrics. This work lays the foundation for the establishment of a widely accepted set of genebank metrics within the global genebank community. Further research and iterative development are necessary to formulate comprehensive metrics applicable to other ex situ conservation methods (such as cryopreservation, in vitro and field conservation) as well as in situ conservation strategies (including genetic reserves and on-farm conservation).

Catches of yellowjackets (Hymenoptera: Vespidae) in traps baited with proteinaceous baits or a heptyl butyrate–based synthetic chemical lure in British Columbia, Canada, and Rio Negro, Argentina, differed among species. In British Columbia, western yellowjackets, Vespula pensylvanica (Saussure), responded preferentially to traps baited with rotisserie chicken (Galliformes: Phasianidae) over canned chicken or canned sardines (Clupeiformes, Clupeoidei), but the synthetic chemical lure was more attractive than rotisserie chicken. Counterintuitively, when rotisserie chicken and the synthetic chemical lure were combined, catches were reduced. In British Columbia, more German yellowjackets, V. germanica Fabricius, were caught in traps baited with rotisserie chicken than with canned chicken, and in Argentina, both German and common, V. vulgaris Linnaeus, yellowjackets preferred sardine-flavoured cat (Carnivora: Felidae) food over the synthetic chemical lure. In British Columbia, northern yellowjackets, V. alascensis (Packard), showed no preference among three types of chicken or between rotisserie chicken and canned sardines. When rotisserie chicken and the synthetic chemical lure were combined, catches of both northern and German yellowjackets were no higher than when traps were baited with rotisserie chicken alone. Resolution of the differential roles of red meat-, poultry-, and fish-based baits, as well as improvement of synthetic chemical lures, would be facilitated by identification of bioactive semiochemicals.

The Indo-Gangetic plains of India have been experiencing erratic weather and unpredictable rainfall patterns for the past 5 years, the average annual rainfall being 1200–1500 mm in the cropping season. Millets are generally grown in this region during the rainy season but environmental fluctuations result in heavy yield penalties in proso millet. Proso millet is one of the preferred crops of this region; however, it is very prone to yield loss due to flooding. Stability studies were conducted to investigate growing proso millet as a catch crop during summer (February–May) as it is a short-duration crop and can fit well in the existing cropping system. This study aims to determine the effect of genotype, environment and their interaction on grain yield and to identify more stable proso millet genotypes suitable for cultivation in the summer season. In total, 63 genotypes were evaluated for various agro-morphological traits for two summer and one kharif seasons. Stability analysis using a GGE biplot revealed that the proso millet performed better in the summer season than in the kharif season. This study has identified summer season as the most ideal environment for multilocation testing of wining genotypes RAU PM- 17, RAU PM-22, RAU PM-35, RAU PM-54, BR-7, TNAU-202 and TNAU-164 which has high stability for the summer season and can be used in breeding programmes as donor parents for traits like grain yield, 1000 grain weight, panicle length and number of productive tillers.