Unbalanced bilinguals often exhibit reduced emotionality in their non-native language, although the underlying neural mechanisms remain poorly understood. This fMRI (functional magnetic resonance imaging) study investigated neural differences during a silent reading task where late Spanish–English bilinguals read happy, fearful and neutral fiction passages in their first (L1) and second (L2) languages. We observed a significant language-by-emotionality interaction in the left hippocampus while participants read fearful texts, indicating a stronger limbic system response in L1. Functional connectivity analyses revealed lower coupling between semantic (left anterior temporal lobe) and limbic (left amygdala) regions when reading fearful texts in L2, suggesting less integrated emotional processing. Overall, these findings show that emotional reading in unbalanced bilinguals is strongly influenced by language, with a higher emotional response and more integrated connectivity between semantic and affective areas in the native language.

Regressive constitutional erosion: the gradual rescue of the traditional gender order through constitutional interpretation – Regressive constitutional reform: rendering explicit the traditional gender order – Preemption: constitutional supremacy and the traditional gender order as a matter of sovereignty and national identity – Constitutional backsliding or problematic constitutional origins to start with

Composed of poikilohydric organisms, biocrusts have the ability to survive during periods of drought, making them particularly important in arid and semi-arid areas. However, despite recent research into climate change, the limits of this tolerance to desiccation and the effects of increased water availability, are not very well known. Our objectives were to analyze the effect of prolonged droughts on the cover and metabolism of various crust types, as well as the effect of increased precipitation. Five types of crusts representative of hypothetic successional stages were studied (Physical, Incipient, Cyanobacteria, Squamarina and Lepraria). Two representative areas were selected for each crust type. Nine plots were established in each area, delimited by a 10-cm-diameter ring, and distributed in sets of three plots. In each set, three treatments were applied (control, watering and rain exclusion), and changes in cover, CO2 fluxes and chlorophyll a fluorescence were analyzed. Rain exclusion led to cover losses due to respiration, although this effect differed among successional stages. However, increased precipitation did not increase biocrust cover, because both photosynthesis and respiration rates increased. Chlorophyll a fluorescence was higher in lichens; under watering, it was not different from the control but decreased under rain exclusion.

Objectives/Goals: Access to accurate public health information is an essential component to ensuring health equity. We launched our social media channels on Instagram, Facebook, and TikTok to highlight, engage with, and bring culturally tailored and language appropriate health and research information to our target communities. Methods/Study Population: Monitoring engagement patterns with our content on each platform influenced the development of a range of innovative campaigns in both English and Spanish that were informed by our core values of inclusivity, trust-building, ongoing bidirectional communication, and co-creation. These three platforms were chosen to ensure reach and engagement with the different demographics within our target populations. The campaigns included those that provided relevant and accurate health information, highlighted the diversity of our team, uplifted our community partners, and gave voice to our community members. This content included health-related infographics, mini-documentary reels, video essays, interviews, and photos. Results/Anticipated Results: We assessed effectiveness, reach, and engagement based on the robustness of the analytics for each platform. Facebook content, the majority of which is in Spanish, appealed more to older, Latino community members. TikTok content appealed more to younger (under 35), primarily English-speaking community members, while Instagram appealed more to organizational partners and community health workers. A 2023 trendline analysis of average monthly Instagram content reach and interactions indicated a moderate-to-strong relationship between our tailored content and audience engagement. Storytelling techniques consistently outperformed other content types across platforms, and community partner collaboration drastically enhanced our visibility, reach, and further validated our approach. Discussion/Significance of Impact: Social media has become increasingly central to bidirectional information dissemination. Implementing tailored strategies and leveraging storytelling techniques is an effective means of engaging diverse audiences, enhancing public health communication, and building and maintaining trust by providing accurate, accessible information.

Objectives/Goals: A limited number of Hispanic researchers compete successfully for NIH career development and research grants. We adapted an established K Club model from the University of Pittsburgh with high success rates to Hispanics in Puerto Rico (PR). The K to R Club’s goal is to increase the successful submission of K- and R-type NIH grants in the HCTRECD Program. Methods/Study Population: K to R Club is an inviting environment that exposes scholars to established funded investigators in PR from all career stages. It creates a forum to discuss different grant mechanisms and explains the selection, submission, and review process. The Club promotes the right mentor selection and mentoring team. It facilitates networking with principal investigators local/external to share their success stories, career development experiences, and grant submission tips. It offers mock review sessions of sections of the grant proposal to provide feedback from invited established investigators during the grant writing process. The Club meets 1–2 times per month in-person or virtual for 1 hour and anonymous evaluations were submitted after each session. Results/Anticipated Results: K to R Club 1st year had 11 sessions with 15 invited speakers. Sessions included: 1 Kickoff, 2 funding opportunities, 2 coaching, 7 successful stories of Diversity Supplement, and F99/K00, K22, K23, K99/R00, R01, and R21 awardees. The highest attendance was for the Kickoff (48). Evaluations response rates ranged from 15 to 62 with the highest participation from women (78% vs. 22% men). Most respondents were PhD (45%) and MD (29%). K to R Club sessions were rated as excellent (84%), 74% agreed that the sessions changed their knowledge very much, and 78% reported it changed their ability to apply for funding very much. Interest in submitting NIH supplements in 12 months was higher (68%) vs. 6 months (48%). Interest in requesting mock reviews for K or R grants in 6 months (91%) vs. 12 months (17%). Discussion/Significance of Impact: The 1st year of the K to R Club had an active attendance and increased the interest in submission of NIH grants. We are working on strategies to increase evaluations’ response rates to improve and address future session needs due to the low response rates recorded. Currently, the semester is full of mock review sessions for grant applications (4 Ks and 1 R01).

Objectives/Goals: This poster describes the scientific rationale, needs assessment, programmatic elements, and impact of a community of practice (CoP) focusing on advancing equity in the science and practice of mentorship. Methods/Study Population: In 2023, the University of Wisconsin Institute for Clinical and Translational Research received NIH R13 funding to host a conference, the Science of Effective Mentorship (Asquith, McDaniels, et.al., 2023). Approximately 150 researchers and program leaders from Clinical and Translational Science Awards (CTSA) Hubs and beyond attended. Data were collected before, during, and after the conference, providing the authors with an initial idea of community needs. As a result, a mentorship CoP was formed. In the subsequent 18 months, a steering and advisory committee established a program of virtual, topic-focused virtual events every 3 months as well as a community website, with increasing attendance and utilization. A survey was disseminated after the completion of one year, and a focus group was held during the last virtual gathering. Results/Anticipated Results: The demand for infrastructure to support a national community of practice will be demonstrated. The demographic and positional diversity (e.g. role within a CTSA Hub) will highlight the opportunities of convening this diverse community. Organizational challenges and opportunities will be highlighted. Assessment data will reveal the broad range of needs and interests of participants. Aggregate demographic, professional, and participation data about community of practice members will be shared, as well as the governance and programmatic elements of this community of practice. Evaluation results from the first year of activity will be displayed. Needs for sustainability will be discussed. Discussion/Significance of Impact: CoPs are not new in the CTR space. Membership in a CoP may reduce isolation individuals feel as they negotiate the important work of equity in the biomedical workforce. Members of this community of practice share the expertise and commitment to promoting equity in the biomedical workforce through supporting robust culture of mentorship.

Homeostats are important to control homeostatic conditions. Here, we have analyzed the theoretical basis of their dynamic properties by bringing the K homeostat out of steady state (i) by an electrical stimulus, (ii) by an external imbalance in the K+ or H+ gradient or (iii) by a readjustment of transporter activities. The reactions to such changes can be divided into (i) a short-term response (tens of milliseconds), where the membrane voltage changed along with the concentrations of ions that are not very abundant in the cytosol (H+ and Ca2+), and (ii) a long-term response (minutes and longer) caused by the slow changes in K+ concentrations. The mechanistic insights into its dynamics are not limited to the K homeostat but can be generalized, providing a new perspective on electrical, chemical, hydraulic, pH and Ca2+ signaling in plants. The results presented here also provide a theoretical background for optogenetic experiments in plants.

Gyrodactylidae Cobbold, 1864 is a monophyletic family of hyperviviparous species, with 36 genera and approximately 700 species. Here, we focused on one of these genera – namely Scleroductus Jara & Cone, 1989 – characterizing its morphological variation systematically and testing its phylogenetic position in relation to other genera in the family. We collected and describe two new species of monogenean parasites of Scleroductus infecting Neotropical freshwater catfishes in Mexico. Scleroductus veracruzano n. sp. infects Guatemalan chulín, Rhamdia guatemalensis in Veracruz, while Scleroductus oaxaqueno n. sp. infects filespine chulín, Rhamdia laticauda in Oaxaca, Mexico. Sequences of the internal transcribed spacer (ITS rDNA) and 18S rDNA were generated for both species. Phylogenetic hypotheses based on maximum likelihood and Bayesian inference analyses of 18S rDNA indicate that the two new Mexican taxa are closely related to each other, and both appear as sister species to an undescribed but molecularly characterized Scleroductus sp. infecting South American catfish, Rhamdia quelen in Brazil. Our analyses confirm the placement of Scleroductus spp. within the monophyletic family Gyrodactylidae, whose sister clade is the Oogyrodactylidae. Within Gyrodactylidae, Scleroductus spp. form a clade sister to a well-supported clade composed by members of the genera Ieredactylus, Gyrodactyloides and Laminiscus.

The Liebau effect generates a net flow without the need for valves. For the Liebau effect pumping phenomenon to occur, the pump must have specific characteristics. It needs tubes with different elastic properties and an actuator to provide energy to the fluid. The actuator periodically compresses the more flexible element. Furthermore, asymmetry is a crucial factor that differentiates between two pumping mechanisms: impedance pumping and asymmetric pumping. In this work, a model based on the fluid dynamics of an asymmetric valveless pump under resonant conditions is proposed to determine which parameters influence the pumped flow rate. Experimental work is used to validate the model, after which each of the parameters involved in the pump performance is dimensionlessly analysed. This highlights the most significant parameters influencing the pump performance such as the actuator period, length tube ratio and tube diameters. The results point out ways to increase a valveless asymmetric pump’s net-propelled flow rate, which has exciting applications in fields such as biomedicine. The model also allows for predicting the resonance period, a fundamental operating parameter for asymmetric pumping.

The fortune jack (Seriola peruana), a pelagic fish typically found along the Tropical Eastern Pacific, has been recorded in the northernmost Gulf of California (GC) region. The first record in the Upper Gulf and habitat expansion of S. peruana is reported based on the meristic, morphometric, and biological data of three specimens caught by local artisanal fishermen in April 2024. The lack of commercial value likely explains the region's absence of records for this species. The increased presence of S. peruana distribution in the Upper GC could have significant ecological implications, which warrants further fish habitat use and climate change research.

Experiments and numerical simulations of inertial particles in underexpanded jets are performed. The structure of the jet is controlled by varying the nozzle pressure ratio, while the influence of particles on emerging shocks and rarefaction patterns is controlled by varying the particle size and mass loading. Ultra-high-speed schlieren and Lagrangian particle tracking are used to experimentally determine the two-phase flow quantities. Three-dimensional simulations are performed using a high-order, low-dissipative discretization of the gas phase while particles are tracked individually in a Lagrangian manner. A simple two-way coupling strategy is proposed to handle interphase exchange in the vicinity of shocks. Velocity statistics of each phase are reported for a wide range of pressure ratios, particle sizes and volume fractions. An upstream shift of the Mach disk in the presence of particles reveals significant two-way coupling even at low mass loading. A semi-analytic model that predicts the extent of the Mach disk shift is presented based on a one-dimensional Fanno flow that takes into account volume displacement by particles and interphase exchange due to drag and heat transfer. The per cent shift in Mach disk is found to scale with the mass loading, nozzle pressure ratio and interphase slip velocity and inversely with the particle diameter.

Objectives: The aim of this study was to analyze the impact of maintaining professional activity on cognitive functioning at advanced ages.

Methods: The sample comprised 131 people aged 60 years-old and older (M = 68,15 years) of whom 41 were still working (30 women and 11 men, Mage = 65.24), and 89 retired (74 women and 15 men Mage = 69.48). To assess participants’ cognitive functioning the following instruments were used: Rey-Osterrieth Complex Figure Test, Digit Span Test, Trail Making Test (TMT), Verbal Fluency Test and Boston Naming Test (Short Form). Since age differences among participants of the two groups were found an ANCOVA test was used, and age was included as a covariable.

Results: Statistically significant differences were found between the two groups in the Part A of TMT (F = 7.383, p < 0.05) and the Boston Naming Test (Short Form) (F = 3.495, p < 0.05). Compared with retired participants those who were still active had better scores on both measures.

Conclusions: Work-related activity in old age has a positive influence on cognitive functioning and can contribute to older people’s cognitive reserve and to maintaining better attention, naming or word retrieval performance. Thus, in order to maintain good cognitive functioning, when older adults retire, they may need to substitute work with stimulating and challenging activities.

Objectives: The aim of this study was to ascertain the influence of a six-month multidomain stimulation program on cognitive functioning.

Methods: The sample comprised 25 older adults 60 years-old and older (Mage = 66.08 years), of whom 96% were women and 54% had high education. The intervention program had a duration of six months and was implemented in a group format in one-hour sessions on a weekly basis. Lessons on neuroprotective factors, training in practical compensatory strategies to improve daily performance, cognitive training exercises and recreational cognitive stimulation activities were included. In order to analyze participants’ cognitive functioning the following instruments were used: Mini-Mental State Examination, Rey Complex Figure Test, Digit Span Test, Stroop Test, Trail Making Test (Part A and Part B), Verbal Fluency Test, Boston Naming Test (Short Form) and Neuropsi Verbal Memory Subtest. Wilcoxon test was performed to test the impact of the intervention program on participants cognitive functioning.

Results: Statistically significant differences were found after the six-month intervention in verbal memory encoding (W= –2.772, p< 0.05), in spontaneous verbal memory retrieval(freerecall) (W= – 3.456, p< 0.001), inverbal recognition memory (W = –3.184, p < 0.001), in spontaneous visual memory retrieval (W = –2.056, p < 0.05), and in naming by visual confrontation (W = –2.521, p < 0.05). In all cases, participants increased their scores after taking part in multicomponent a six-month multidomain stimulationprogram

Conclusions: One of the biggest concerns among older people is the loss of cognitive abilities that may occur with ageing. Cognitive stimulation programs, if sufficiently comprehensive, can be a good tool to maintain and improve cognitive functions as we age.

Recently, women’s presence on top boards of directors has significantly increased, challenging the long standing of male-led corporate elites. In light of the still-developing literature, this article provides a century-long examination of women’s entry into the Spanish corporate elite, offering several original contributions. In addition to its pioneering input into the country’s historiography, the work uses a holistic model to introduce a comparative European approach. Moreover, it empirically examines the significant yet previously unexplored impact of elite training institutions on the advancement of female directors as well as their arrival through a national holding company and their presence in leading publicly traded companies. Findings showed four distinct stages in their trajectory: discriminatory exclusion, during the first third of the twentieth century; exceptional inclusion, with early positions in their family-owned firms; gradual incorporation, with increased political representation and expanded academic access in the latter decades of the last century; and promotion, supported by twenty-first-century political strategies, while still revealing the handicap of women’s delayed entry into the corporate network.