The COVID-19 pandemic disrupted the population’s lives. Stressful conditions during the lockdown and the reintroduction to a changed social environment emotionally affected children and adolescents. The aim of this work was to study anxiety and depressive symptoms in Italian, Spanish, and Portuguese children and adolescents aged 3 to 18 years at different moments of the COVID-19 pandemic: April 2020 (during confinement), September 2020 (with the schools’ reopening), and September 2023 (with the situation restored). Parents of 1,097 children participated in at least one assessment, completing measures of child emotional symptoms online. Cases with subclinical symptoms of anxiety and depression were higher compared to pre-pandemic studies. Overall, anxiety increased from April 2020 to September 2020, decreasing in September 2023 with no differences compared to the first assessment. Depression was high in April 2020 but decreased in September 2020, with no significant differences three years later, in September 2023. Cross-country comparisons at each point are discussed. Moreover, boys showed higher levels of depression during the pandemic compared to girls. Older children, compared to younger ones, had more anxiety and depressive symptoms throughout all the moments. These findings highlight the emotional impact of the pandemic and its conditions on children and adolescents.

The COVID-19 pandemic significantly challenged the mental health of children and adolescents, with existing research highlighting the negative effects of restrictive measures to control the virus’s spread. However, in the specific context of this pandemic, there is limited understanding of how these difficulties have persisted over time after the situation was fully restored. This study sought to evaluate the pandemic’s impact on psychological symptoms in children from Italy, Spain, and Portugal across five-time points (2, 5, and 8 weeks, 6 months, and three and a half years after the pandemic’s onset). A total of 1613 parents completed the Psychological Impact of COVID-19 and Confinement on Children and Adolescents Scale, reporting symptoms in their children aged 3–17 years (39.2% female). The findings reveal an initial surge in psychological difficulties—anxiety, mood, sleep, behavioral, eating, and cognitive disturbances—followed by improvements in these domains three and a half years later. By September 2023, Spanish children experienced more significant reductions in symptoms compared to their Italian and Portuguese peers. While the COVID-19 pandemic has been a prolonged crisis, with varying impacts over time and across regions depending on the strictness of restrictions, the trends suggest a gradual improvement in the psychological well-being of children and adolescents.

Late Quaternary fluvial channel deposits are notoriously difficult to date. In the midwestern United States, shells of aquatic mollusks can be found within many fluvial channel sediments and therefore can be radiocarbon (14C) dated to determine the age of the deposits. However, carbonate platform rocks are abundant in this region, potentially causing freshwater 14C reservoir effects (FRE) in mollusk shells. We 14C dated 11 aquatic gastropod and bivalve shell samples from specimens collected live from a stream in southwestern Ohio during three different years to assess the modern 14C reservoir effect. Modern samples yielded an average 14C FREmodern of 518 ± 65 14C yrs for 2020 (n=5), 640 ± 34 14C yrs for 2021 (n=2), and 707 ± 76 14C yrs for 2022 (n=4). We also 14C dated matched pairs of organic wood or charcoal and aquatic mollusk shells from late Pleistocene and Holocene deposits in the Four Mile Creek floodplain to determine the FREfossil. These samples, free of any potential influence from nuclear bomb testing, yielded an overall weighted mean FREfossil of 1029 ± 345 14C yrs. We then assess the advantages and limitations of both the FREmodern and FREfossil methods for determining freshwater reservoir effects. Finally, we apply the FREfossil correction to a series of shell ages from fluvial terrace deposits as a case study. The results indicate that although there is a 14C FRE in streams from the midwestern United States, aquatic shells can provide robust age control on fluvial channel deposits. More research is needed to understand the spatial and temporal variability of FREs, as well as any species effects, among various watersheds across the midwestern United States.

Detailed sedimentological, geochemical, palynological, and aquatic-microfossil analyses on a new composite profile (Para-Reference Profile Schöningen 13 II and Zeugenblock 13 II [2018]) exposed at the archaeological excavation site of Schöningen 13 II reflect several phases of newly recorded lake level fluctuations and vegetation changes. A pronounced deforestation and the expansion of grasses and herbal plant communities characterize the first steppe (open woodland) phase, which follows the interglacial forest communities. A succeeding tripartite woodland phase predominantly marked by Betula and Pinus is followed by another rather dry steppe phase and a second woodland period, which includes the famous archaeological “spear horizon” 13 II-4ab. Transition into a cold period is indicated by progressive desiccation of the lake and a shift to a steppe/tundra vegetation. Novel 3D images of the 10 most characteristic phases of the Reinsdorf sequence have been constructed based on the botanical data (macro remains as well as palynomorphs), terrestrial vertebrate faunal, geochemical, sedimentological, and previously established data from aquatic microfossils. In addition, a tentative correlation between the post-interglacial phases of the Reinsdorf sequence and Marine Isotope Substages 9d–9a, based on biostratigraphical as well as sedimentological data, is proposed.

Domestic cats (Felis catus) are widely believed to be highly sensitive to the effects of social stress, especially when living in high density populations. Cats are capable of adapting to living in a group, but this will often require opportunities for escaping and hiding. In this pilot study, adrenocortical activity, as a valuable physiological indicator of arousal underpinning potential emotional stress, was evaluated through the measurement of mean faecal glucocorticoid metabolites (mGCM) in fourteen singly and sixteen group-housed cats. Living conditions and ratings of the owners’ quality of life (evaluated from self-reported questionnaires) were used as factors associated with faecal glucocorticoid levels of the cats. A direct association between the scores of owners’ social dimension of quality of life and the cats’ mGCM was found for single cats only, with higher owner social scores associated with higher cat mGCM. No significant differences in mGCM were found between singly versus group-living cats. This suggests that the under-explored factor of owner lifestyle could play an important role in domestic cats’ day-to-day levels of arousal, especially when kept as single pets.

On September 19, 1922, Beulah M., a thirty-year-old cook, saved a “small child from a vicious cow on Angola.” This event occurred only a few months after her admission to the Louisiana State Penitentiary (LSP), where she was serving a life sentence for alleged murder. The infant was one of the many of the white prison staff's children raised on the penitentiary plantation nestled in a large meander of the Mississippi river. This happy-ending drama featuring a Black woman prisoner and a free white child arose from the “cohabitation” of free white households within the incarcerated population. The incident, quite unexpected in a carceral setting, prompted the penitentiary general manager to place Beulah M. on the “eligibility list” for parole and to grant her “full single good time for meritorious service,” which meant the possibility of an earlier release by a few months. Beulah's action might also have motivated authorities to assign her to be “servant” in the Camp D Captain's house in July 1923, and later to be a nurse in the nine-bedroom “Big House,” occupied by one of the penitentiary staff of higher rank. The peculiar nature of her alleged crime, the beating to death of her seven-year-old Black step-daughter, was apparently not perceived as a deterrent to entrust her to care for white children. Her courageous action toward a white child at Angola might even have been a compelling argument for her early pardon and discharge, which she received only after nine years at Angola, although her plea for a pardon had been rejected at least once before. Beulah M.'s story is the story of a coerced African American domestic laborer in white homes, rewarded for her perceived subservience to the Jim Crow order. It exemplifies one aspect of Black women's experiences of hard labor for the state of Louisiana during the first half of the twentieth century.

This study looks at the role of hiking in the development and the identity of a French region. Both rural and agricultural, Brittany saw a reorganisation of its territory during the 1970s, partly due to activities such as hiking. By becoming a political focus, the activity contributed to making paths, only previously used for field labour, a tool for territorial development. It also rejuvenated Brittany’s regional identity as it explored a forgotten heritage. The course of action adopted aimed to bring new populations, particularly urban ones, to rural areas. Behind the attention focused on paths, as an essential requirement for hiking, lay a concern for environmental and social sustainability with effects on lifestyles, tourism, and the shaping of a region in transition.

Dietary supplements have been increasingly used by gym users and are often consumed without the guidance of a health professional. Moreover, the indiscriminate supplements use can have adverse health effects, such as changes in liver and kidney function. The aim of this study was to verify the association between dietary supplements intake with alterations in the liver and kidney function among gym users. A cross-sectional study was conducted with 594 gym users (mean age 37 (sd 14) years, 55·2 % women) from a city in southern Brazil. A questionnaire was used to evaluate the use of dietary supplements. The markers of the liver (alanine aminotransferase, aspartate aminotransferase (AST), alkaline phosphatase, γ-glutamyltransferase) and renal (creatinine and urea) function were also evaluated on a subsample of the study population. Data were analysed by binary logistic regression, adjusted for sex, age and education. The prevalence of dietary supplement intake was 36·0 %. Individuals who intake dietary supplements showed a higher prevalence to present slight alterations in the AST enzyme and in the urea after adjustments for potential confounders. In conclusion, the use of dietary supplement was associated with slight alterations in AST enzyme and in the urea among gym users. These findings show the importance of using supplements correctly, especially with guidance from professionals trained to avoid possible risks to health.

Recent studies have shown the oxygen isotopic composition (δ18O) of modern terrestrial gastropod shells is determined largely by the δ18O of precipitation. This implies that fossil shells could be used to reconstruct the δ18O of paleo-precipitation as long as the isotopic system, including the hydrologic pathways of the local watershed and the gastropod systematics, is well understood. In this study, we measured the δ18O values of 456 individual gastropod shells collected from paleowetland deposits in the San Pedro Valley, Arizona that range in age from ca. 29.1 to 9.8 ka. Isotopic differences of up to 2‰ were identified among the four taxa analyzed (Succineidae, Pupilla hebes, Gastrocopta tappaniana, and Vallonia gracilicosta), with Succineidae shells yielding the highest values and V. gracilicosta shells exhibiting the lowest values. We used these data to construct a composite isotopic record that incorporates these taxonomic offsets, and found shell δ18O values increased by ~4‰ between the last glacial maximum and early Holocene, which is similar to the magnitude, direction, and rate of isotopic change recorded by speleothems in the region. These results suggest the terrestrial gastropods analyzed here may be used as a proxy for past climate in a manner that is complementary to speleothems, but potentially with much greater spatial coverage.

A low-cost technique named ‘on-farm’ seed priming is increasingly being recognized as an effective approach to maximize crop establishment. It consists of anaerobically soaking seeds in water before sowing resulting in rapid and uniform germination, and enhanced seedling vigour. The extent of these benefits depends on the soaking time. The current determination of optimal soaking time by germination assays and mini-plot trials is resource-intensive, as it is species/genotype-specific. This study aimed to determine the potential of the seed respiration rate (an indicator of metabolic activity) and seed morphological changes during barley priming as predictors of the priming benefits and, thus, facilitate the determination of optimal soaking times. A series of germination tests revealed that the germination rate is mostly attributable to the rapid hydration of embryo tissues, as the highest gains in the germination rate occurred before the resumption of respiration. Germination uniformity, however, was not significantly improved until seeds were primed for at least 8 h, that is, after a first respiration burst was initiated. The maximum seedling vigour was attained when the priming was stopped just before the beginning of the differentiation of embryonic axes (20 h) after which vigour began to decrease (‘over-priming’). The onset of embryonic axis elongation was preceded by a second respiration burst, which can be used as a marker for priming optimization. Thus, monitoring of seed respiration provides a rapid and inexpensive alternative to the current practice. The method could be carried out by agricultural institutions to provide recommended optimal soaking times for the common barley varieties within a specific region.

From the prevention of natural disasters such as landslide and avalanches, to the enhancement of energy efficiencies in chemical and civil engineering industries, understanding the collective dynamics of granular materials is a fundamental question that are closely related to our daily lives. Using a recently developed multi-static radar system operating at 10 GHz (X-band), we explore the possibility of tracking a projectile moving inside a granular medium, focusing on possible sources of uncertainties in the detection and reconstruction processes. On the one hand, particle tracking with continuous-wave radar provides an extremely high temporal resolution. On the other hand, there are still challenges in obtaining tracer trajectories accurately. We show that some of the challenges can be resolved through a correction of the IQ mismatch in the raw signals obtained. Consequently, the tracer trajectories can be obtained with sub-millimeter spatial resolution. Such an advance can not only shed light on radar particle tracking, but also on a wide range of scenarios where issues relevant to IQ mismatch arise.

A key concern regarding current and future climate change is the possibility of sustained droughts that can have profound impacts on societies. As such, multiple paleoclimatic proxies are needed to identify megadroughts, the synoptic climatology responsible for these droughts, and their impacts on past and future societies. In the hyperarid Atacama Desert of northern Chile, many streams are characterized by perennial flow and support dense in-stream wetlands. These streams possess sequences of wetland deposits as fluvial terraces that record past changes in the water table. We mapped and radiocarbon dated a well-preserved sequence of in-stream wetland deposits along a 4.3-km reach of the Río San Salvador in the Calama basin to determine the relationship between regional climate change and the incision of in-stream wetlands. The Río San Salvador supported dense wetlands from 11.1 to 9.8, 6.4 to 3.5, 2.8 to 1.3, and 1.0 to 0.5 ka and incised at the end of each of these intervals. Comparison with other in-stream wetland sequences in the Atacama Desert, and with regional paleoclimatic archives, indicates that in-stream wetlands responded similarly to climatic changes by incising during periods of extended drought at ~9.8, 3.5, 1.3, and 0.5 ka.

Continental paleoclimate proxies with near-global coverage are rare. Land snail δ18O is one of the few proxies abundant in Quaternary sediments ranging from the tropics to the high Arctic tundra. However, its application in paleoclimatology remains difficult, attributable in part to limitations in published calibration studies. Here we present shell δ18O of modern small (<10 mm) snails across North America, from Florida (30°N) to Manitoba (58°N), to examine the main climatic controls on shell δ18O at a coarse scale. This transect is augmented by published δ18O values, which expand our coverage from Jamaica (18°N) to Alaska (64°N). Results indicate that shell δ18O primarily tracks the average annual precipitation δ18O. Shell δ18O increases 0.5–0.7‰ for every 1‰ increase in precipitation δ18O, and 0.3–0.7‰ for every 1°C increase in temperature. These relationships hold true when all taxa are included regardless of body size (ranging from ~1.6 to ~58 mm), ecology (herbivores, omnivores, and carnivores), or behavior (variable seasonal active periods and mobility habits). Future isotopic investigations should include calibration studies in tropical and high-latitude settings, arid environments, and along altitudinal gradients to test if the near linear relationship between shell and meteoric precipitation δ18O observed on a continental scale remains significant.

Biofilms can damage implants and are difficult to treat. Here, we assessed the performance of a tripeptide that self-assembles into an antifouling coating over a broad range of shear conditions that are relevant to biomedical applications. Adhesion assays were performed using a parallel plate flow chamber. The results show that the coating can reduce Escherichia coli adhesion up to 70% when compared with glass. At a shear rate of 15/s, typical for urinary catheters, the coating reduced the adhesion by more than 50%. These findings suggest critical features that should be considered when developing surfaces for biomedical purposes.

Playas, or ephemeral lakes, are one of the most common depositional environments in arid and semiarid lands worldwide. Playa deposits, however, have mostly been avoided as paleoclimatic archives because they typically contain exceptionally low concentrations of organic material, making 14C dating difficult. Here, we describe a technique for concentrating organic matter in sediments for radiocarbon dating and apply it to playa sediments recovered from a 2.35 m sediment core from a small playa in southern Jordan. Based on 14C ages of the organic concentrate fraction, the playa was active from ~29 to 21 ka, coincident with the last major high stand of Paleolake Lisan and wet conditions recorded by other paleoclimatic proxies in the southernmost Levant during the last full glacial period (35–20 ka). The timing and spatial pattern of these records suggests that the increased moisture was likely derived from more frequent and deeper eastern Mediterranean (EM) cyclones associated with the intensification of the westerlies. The presence of full glacial pluvial deposits in southern Jordan (29°N), and the lack of similarly aged deposits in the northern Arabian Peninsula to the south, suggests that the southerly limit of the incursion of EM cyclones during last full glacial period was ~28°N.