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The migratory phase is a critical time for Fasciola hepatica as it must locate, penetrate and migrate through the alimentary tract to the liver parenchyma whilst under attack from the host immune response. Here, scanning and transmission electron microscopy were used to monitor the in vitro effects of sera (with, and without, complement depletion) on F. hepatica newly excysted juveniles (NEJs) and flukes recovered at 7, 35, 70 and 98 days post infection (dpi) from the liver and bile ducts of rats. Test sera were from these F. hepatica-infected rats. A F. hepatica NEJ-specific rabbit antiserum was also used. All fluke stages demonstrated release of the tegumental glycocalyx and microvesicles and intense activity within the tegumental syncytium characterized by eccrine secretion of T-0/T-1/T-2 secretory bodies with subsequent microvillar formation and shedding of microvesicles from the apical plasma membrane. Exposure of both NEJs and 35 dpi flukes to 35 and 70 dpi rat sera produced significant amounts of eccrine-derived secretory material and putative attached immunocomplex. Rabbit anti-F. hepatica NEJ-specific antiserum produced similar responses at the NEJ tegument, including binding of putative immunocomplex to the surface, but with additional blistering of some regions of the apical plasma membrane. Our data suggest that immune sera stimulates multiple interrelated secretory mechanisms to maintain the integrity of the tegumental barrier in response to immune attack. Concurrent release of microvesicles may also serve to both divert the immune response away from the fluke itself and permit delivery of immunomodulatory cargo to immune effector cells.
Polycephalic larvae of Taenia endothoracicus were found from naturally infected gerbils Meriones lybicus in a rural area of Arak, central Iran. A large cyst containing 19 protoscolices was located in the peritoneum, attached to the large omentum. The characteristics of the protoscolices and rostellar hooks confirmed the identification of these larvae as T. endothoracicus.
At the institutional and individual level, interest is growing in theatre and performance studies digital humanities (DH) projects. Too often, this interest fizzles out in the leap from digital imaginings to production timelines that real people must execute (with real budgets). A thorough understanding of the labor structures that drive such projects is necessary in developing realistic, sustainable models of DH work.
In this paper, the accuracy and practical capabilities of three different reduced-order models (ROMs) are explored: an enhanced implicit condensation and expansion (EnICE) model, a finite element beam model, and a finite volume beam model are compared for their capability to accurately predict the nonlinear structural response of geometrically nonlinear built-up wing structures. This work briefly outlines the different order reduction methods, highlighting the associated assumptions and computational effort. The ROMs are then used to calculate the wing deflection for different representative load cases and these results are compared with the global finite element model (GFEM) predictions when possible. Overall, the ROMs are found to be able to capture the nonlinear GFEM behaviour accurately, but differences are noticed at very large displacements and rotations due to local geometrical effects.
Whole-rock major- and trace-element data are presented on a sample collection from the >3 Ga Amikoq Layered Complex (ALC), and hosting amphibolites within the Mesoarchean Akia terrane, SW Greenland. The lithologies range from leuconorite to melanorite/feldspathic orthopyroxenite, orthopyroxenite to harzburgite through to dunite, and tholeiitic basaltic–picritic mafic host rocks. The Amikoq Layered Complex samples are primitive (Mg#: 65–89) with elevated Ni and Cr contents. However, the absence of troctolitic lithologies and the presence of two orthopyroxene compositional trends, suggests that the successions might not be comagmatic. On the basis of trace-element cumulate models, relatively low Ni contents and minor negative Sr-Eu anomalies in some high-Ti ultramafic rocks, it is not possible to exclude a petrogenesis related to a melt similar to that of the mafic host-rocks. Ultramafic samples with U-shaped trace-element distribution patterns are petrogenetically related to the noritic sequences, either through cumulus mineral accumulation or melt-rock reactions. Assimilation-fractional-crystallisation modelling of melanorites nevertheless require the parental melt to have been contaminated/mixed with a component of island-arc-like tholeiite affinity. A boninite-like parental melt might have been derived from the subcontinental lithospheric mantle of the Akia terrane, or alternatively via assimilation of an ultramafic parental melt with island-arc-like tholeiite. Given the complex geological evolution and high-grade metamorphic overprint of the Amikoq Layered Complex, we are unable to differentiate between the two models.
Maternal exercise has shown beneficial effects on mother and child. Literature confirm progeny’s cognition improvement, and upregulation in neurotrophins, antioxidant network, and DNA repair system. Considering that there is a lack of information demonstrating the impact of maternal exercise on offspring’s skeletal muscle, we aimed to investigate the mitochondrial and redox effects elicited by maternal swimming. Adult female Wistar rats were divided into three groups: control sedentary, free swimming, and swimming with overload (2% of the body weight). Exercised groups were submitted weekly to five swimming sessions (30 min/day), starting 1 week prior to the mating and lasting to the delivery. Gastrocnemius and soleus muscle from 60-day-old offspring were analyzed. Our results clearly showed a sex-dependent effect. Male soleus showed increased mitochondrial functionality in the overload group. Female muscle from the overload group adapted deeply. Considering the redox status, the female offspring delivered to overload exercised dams presented reduced oxidants levels and protein damage, allied to downregulated antioxidant defenses. We also observed an increase in the mitochondrial function in the gastrocnemius muscle of the female offspring born from overload exercised dams. Soleus from female delivered to the overload exercise group presented reduced mitochondrial activity, as well as reduced reactive species, protein carbonyls, and antioxidant network, when compared to the male. In conclusion, maternal exercise altered the redox status and mitochondrial function in the offspring’s skeletal muscle in a sex-dependent way. The clinical implication was not investigated; however, the sexual dimorphism in response to maternal exercise might impact exercise resilience in adulthood.
The metamorphic history of the Mesoarchean Amikoq Layered Complex within the Akia terrane of SW Greenland was characterised by electron microprobe mineral data and detailed petrography on 12 representative samples, integrated with zircon U–Pb geochronology and petrology. The complex intruded into a >3004 Ma supracrustal association now consisting of granoblastic metabasites with subordinate quartz-rich gneiss. Supracrustal host rocks contain a relict high-temperature assemblage of orthopyroxene–clinopyroxene (± pigeonite exsolution lamellae, exsolved at ~975–1010°C), which is interpreted to pre-date the Amikoq intrusion. Cumulate to granoblastic-textured rocks of the main Amikoq Layered Complex range modally from leuconorite to melanorite, orthopyroxenite to harzburgite/dunite and rare hornblende melagabbro. Observed mineralogy of main complex noritic lithologies is essentially relict igneous with orthopyroxene–biotite and hornblende–plagioclase thermometers yielding temperatures of ~800–1070°C. An anatectic zircon megacryst from a patchy quartzo–feldspathic leucosome hosted in an orthopyroxene-dominated Amikoq rock reflects local anatexis at peak metamorphic P–T conditions and yields an intrusion minimum age of 3004 ± 9 Ma. Field observations indicate local anatexis of orthopyroxene-dominated lithologies, possibly indicating a post-intrusion peak temperature of >900°C. The last preserved stages of retrogression are recorded in paragneiss plagioclase–garnet, biotite–garnet and host rock ilmenite–magnetite pairs (≤3 kbar and ~380–560°C).
The Amikoq Complex intruded a MORB-like crustal section and the former remained relatively undisturbed in terms of modal mineralogy. Preservation of igneous textures and mineralogy are related to an anhydrous, high-grade metamorphic history that essentially mimics igneous crystallisation conditions, whereas local high-strain zones acted as fluid pathways resulting in hydrous breakdown of igneous minerals. There is no evidence of equilibration of the intrusion at sub-amphibolite-facies conditions.
Arma uirumque cano … ‘Je chante les armes et l'homme …’ ainsi commence l’Énéide, ainsi devrait commencer toute poésie.
It is far from an overstatement to make the claim that in the surviving corpus of Latin poetry no phrase is more immediately identifiable than the pronouncement of the Virgilian narrator on the ‘arms and the man’ of his subject matter. The presence of arma uirumque in a particular formation cannot fail to put us in mind of the Aeneid and its concomitant ideological associations. A consequence of the epic's centrality as a canonical text was the emergence in antiquity of arma uirumque as a synecdochic quotation for the work as a whole and, as such, for the figure of the poet himself. This transformation was further actuated by the ancient practice of ascribing titular authority to a poem's incipit, ensuring that arms and the man took on especial resonance. Even within the Aeneid, self-referential intratextual play with the Virgilian utterance can be detected. Furthermore, in post-Augustan verse, Fowler argues that arma alone is always loaded in a metaliterary fashion, serving as an identifying marker for Virgil's epic or, more broadly, for epic in general. This was so marked a phenomenon that the opening word of the poem was recycled and reworked by Ovid around a decade after the compositional beginnings of the Aeneid, in the first book of the Amores, in what formed an announcement of the redeployment of epic arma into the elegiac world of militia amoris (Am. 1.1–2).
The objective of the study was to determine the cost effectiveness of long-term maintenance treatment with fluvoxamine compared with tricyclic antidepressants (TCAs) in the treatment of patients with depressive disorders.
A Markov process model was used to model mental health status and economic outcomes as they accrued over a hypothetical 5-year follow-up period. The main outcome measures were time without depression, direct and indirect costs. The clinical data were obtained from specially conducted pharmacoepidemiological study of patients with depressive disorders who were prescribed long-term antidepressive treatment in two Moscow outpatient psychiatric clinics; naturalistic study of clinico-social effectiveness of fluvoxamine use for long-term (6 month) maintenance treatment; results of cost-analysis study of depressive disorders; data from published literature.
The results showed the increase of medical expenses in case of fluvoxamine not less than in 1,4 times compared to TCAs and gain of 110 days without disease. In addition it was shown that long-term treatment with fluvoxamine is associated with a mean increase time without depression of 7%. The total costs of maintenance treatment with fluvoxamine (50 mg/day) were substantially lower than with TCAs. When social perspectives were taken into account it was shown that fluvoxamine is recourse-saving and expenses on the cost of the drug are covered by social effect of therapy. Sensitivity analysis confirmed the robustness of these results.
In conclusion, the study demonstrates that long-term maintenance treatment with fluvoxamine is both more effective and less costly (from social perspectives) than treatment with TCAs for patients with depressive disorders.
As the elderly population is growing world-wide, depression in the elderly is becoming an increasing health problem. Studies of depression shows varying results in development patterns with age (1, 2).
Aims
To study changes in prevalence and new cases of depression in elderly age cohorts.
Methods
The Nord-Trøndelag Health Survey (HUNT) is a general health survey conducted in Nord-Trøndelag County with a follow-up after 11 years. The participants aged 45 years or above at baseline were included. Depression was covered by the depression sub-scale of the Hospital Anxiety and Depression Scale (HADS-D; 3). Prevalence and incidence of depression was estimated in the different age cohorts.
Results
16517 participants were included and divided in age cohorts based on their age at baseline. A significant increase in depression was found in all age cohorts from 76 years at follow-up. The oldest age cohort (86–90 years at follow-up) had the greatest increase with a change from 11.3% to 20.9%. New cases of depression is increasing with age, with over 10% new cases in all age cohorts aged 81 years and above at follow-up.
Conclusions
We found increased prevalence of depression and a large number of new cases of depression in the oldest age cohorts.
To assess the safety and effectiveness of umbilical blood stem cells (UBSC) regarding neurocognition and social cognition in schizophrenic patients.
Samples and methods:
10 men (33,6±10,6 years old) with episodic type of paranoid schizophrenia in remission with dominating negative symptomatic (F20.04 in ICD-10), who gave the informed consent and received concentrate of 250 millions UBSC intravenously. the control sample (10 patients) meeting the key demographic and disorder criteria received placebo. All patients continued antipsychotic therapy in fixed doses. in the first phase, both samples previously examined against PANSS and MCCB received one injection of UBSC or placebo. in the second phase, ten patients received four injections of UBSC each, one injection every two weeks. the dynamic was rated before each injection and at the 3rd, 5th, 7th, and 12th week after the last injection.
Results:
The date of the first phase points to a safety of UBSC injections (no serious or severe adverse events registered). the second phase proves the effectiveness of UBSC: the PANSS score drops from 24.5±2.4 to19.2±2.7(р< 0,001)on the negative psychopathology scale, and 41.2±6.8 to 30.82±4.4 on the general psychopathology scale. the neurocognition improved (р< 0,001) in speed of processing (30.6±1.6 to 49.4±1.7), attention (30.6±2.4 to 48.5±3.1), memory (39.7±1.4 to 56.1±1.4), verbal learning (41.3±1.7 to 62±3.2), visual learning (38.6±3.4 to 62.9±2.5), executive functions (35±2.2 to 59.5±2.5). However, the increase in social cognition was statistically nonsignificant (43.6±1.6 to 46.1±2.1).
Conclusion:
The cognitive impact of UBSC appears to be made via its obvious metabolic (nootropic) and psychostimulant actions.
Most suicide attempters are referred to community health professionals (CHP) after discharge from emergency department (ED). Thereafter, outpatient treatment engagement (OTE) is often poor. Strengthening it seems likely to reduce the risk of repeat suicidal behaviour.
Objectives
To identify the predictive factors of OTE.
Methods
Multicentre prospective study in true life conducted in adult patients (≥18) in 4 EDs of the Southern Paris region. Patients were referred to CHP. OTE was assessed by telephone calls at 1 and 3 months. OTE at 1 month was defined as having booked 1 appointment; OTE at 3 months as having attended 2 appointments and booked another one.
Results
One hundred and fifty-five patients were assessed at 1 month and 144 at 3 months. OTE at 1 and 3 months was observed in 58% and 51% respectively. A multivariate analysis showed factors significantly predictive of OTE at 1 month: a psychiatric follow-up before the suicide attempt (SA), ≥2 psychiatric interviews during the stay at ED, appointment with CHP booked before discharge from the ED; and at 3 months: college or university education, absence of alcohol intake during the SA, psychiatric follow-up before the SA, appointment with CHP booked before discharge from the ED.
Conclusions
This multicentre prospective study highlights the benefit of booking outpatient appointment before discharge from ED; of allowing patient to benefit from at least two psychiatric interviews; of being particularly cautious with patients who took alcohol concomitantly to their SA, patients with low level of academic achievement and patients without psychiatric follow-up.
Disclosure of interest
The authors have not supplied their declaration of competing interest.
Self-efficacy is the belief or personal confidence related to the own ability to perform a specific action necessary to obtain a certain result. Its use refers to the application of validated instruments.
Aims
Describe the process of cross-cultural adaptation of the drug-taking confidence Questionnaire (DTCQ-8) drugs’ version and to verify its content validity and reliability.
Methods
It's a methodological study accomplished by nine experts to the process of adaptation and validation and had a sample of 40 drug users in treatment in the Center of Psycho-Social Attention for alcohol and other drugs, Recife, Pernambuco, Brazil.
Results
The mean index semantics’ agreement (0.989; 0.989; 1.00), idiomatic (0.967), experiential (0.956), conceptual (0.978) and content validation on the clarity of language (0.972), practical relevance (0.958), the theoretical importance (0.958) and theoretical dimension (1.00) demonstrated a satisfactory process. The clinical validation showed that 57.5% of users were classified as moderate on the self-efficacy item to resist the urge to use drugs in high-risk situations, and the Cronbach's alpha coefficient was 0.889 for the complete instrument, ranging from 0.863 to 0.890 among its items.
Conclusions
DTCQ-8 drugs proved to be easy to apply and understand. Its adaptation process was satisfactory for the application in the Brazilian context. The results showed that in this sample, this instrument was suitable to measure the Brazilian user's self-efficacy to resist the urge to consume these substances in high-risk situations.
Disclosure of interest
The authors have not supplied their declaration of competing interest.
Knowledge on the reproductive biology of target insect pest is essential for the effective implementation of pheromone-based pest management tactics. In mealybugs, the second largest family of scale insects, the existence of female multiple mating was recently suggested. In this study, we aimed at testing how general is this behavior in mealybugs, by investigating polygyny and polyandry in two cosmopolitan pest mealybugs, Planococcus citri and Pseudococcus calceolariae. Males of these species were able to mate an average of 11.9 and 13.3 females, respectively, during their lifespan. The number of fertilized females per male decreased with male age/mating history for both mealybugs. We found no differences in female fecundity and fertility, when fertilized by males with different mating history. When we used male age as a proxy of mating history, we observed a significant negative effect on female fecundity. The females of both species remained receptive after first copula and eventually mated multiple times. The percentage of remated females of P. citri decreased linearly with time since first copula, with some maintaining receptivity up to 24 h. Males showed no preference between virgin and mated females, in static-air olfactometer tests. We found no benefit of female multiple mating in relation to fecundity. In biparental mealybugs, the mating system of males is possibly scramble competition polygyny; and that of females is possibly polyandry, with female receptivity restricted to a relatively short period. We discuss the practical implications of the results for pest management.
In this paper an algorithm for ice/water classification of C- and L-band dual polarization synthetic aperture radar data is presented. A comparison of the two different frequencies is made in order to investigate the potential to improve classification results with multi-frequency data. The algorithm is based on backscatter intensities in co- and cross-polarization and autocorrelation as a texture feature. The mapping between image features and ice/water classification is made with a neural network. Accurate ice/water maps for both frequencies are produced by the algorithm and the results of two frequencies generally agree very well. Differences are found in the marginal ice zone, where the time difference between acquisitions causes motion of the ice pack. C-band reliably reproduces the outline of the ice edge, while L-band has its strengths for thin ice/calm water areas within the icepack. The classification shows good agreement with ice/water maps derived from met.no ice-charts and radiometer data from AMSR-2. Variations are found in the marginal ice zone where the generalization of the ice charts and lower accuracy of ice concentration from radiometer data introduce deviations. Usage of high-resolution dual frequency data could be beneficial for improving ice cover information for navigation and modelling.
Trichostrongylus spp. are primarily parasites of ruminants, but humans can become infected as accidental hosts. Information about the clinical aspects of human trichostrongyliasis is limited. This study investigated the clinical and haematological characteristics of a large number of trichostrongyliasis patients. In the Fouman district of Guilan Province in northern Iran, during 2015–2016, 60 patients were identified as positive for Trichostrongylus spp., using stool examination methods. The clinical manifestations and demographic data of all patients were recorded and further analysed. Twenty-three patients (38.3%) were male and 37 (61.7%) were female. Among the individuals infected only with Trichostrongylus, only nine patients (16.4%) were asymptomatic. Forty-six patients (83.6%) presented with gastrointestinal (76.3%), pulmonary (30.9%) and cutaneous (12.7%) symptoms. No statistically significant relationship was found between clinical manifestations and sex or age groups. Ten patients (18.1%) revealed eosinophilia and five (9.1%) presented with hypochromic microcytic anaemia. The relationship between eosinophilia and age group, sex and clinical manifestations showed no statistical significance. Our study indicated that trichostrongyliasis may be a major parasitic aetiology for gastrointestinal symptoms and eosinophilia in rural residents of endemic areas.
Mal de Río Cuarto virus (MRCV, Fijivirus, Reoviridae) causes one of the most important diseases in maize (Zea mays L.) in Argentina and has been detected in mixed infections with a rhabdovirus closely related to Maize yellow striate virus. In nature both viruses are able to infect maize and several grasses including wheat, and are transmitted in a persistent propagative manner by Delphacodes kuscheli Fennah (Hemiptera: Delphacidae). This work describes the interactions between MRCV and rhabdovirus within their natural vector and the consequences of such co-infection regarding virus transmission and symptom expression. First- and third-instar D. kuscheli nymphs were fed on MRCV-infected wheat plants or MRCV-rhabdovirus-infected oat plants, and two latency periods were considered. Transmission efficiency and viral load of MRCV-transmitting and non-transmitting planthoppers were determined by real-time quantitative polymerase chain reaction analysis (RTqPCR). Vector transmission efficiency was related to treatments (life stages at acquisition and latency periods). Nevertheless, no correlation between transmission efficiency and type of inoculum used to infect insects with MRCV was found. Treatment by third-instar nymphs 17 days after Acquisition Access Period was the most efficient for MRCV transmission, regardless of the type of inoculum. Plants co-infected with MRCV and rhabdovirus showed the typical MRCV symptoms earlier than plants singly infected with MRCV. The transmitting planthoppers showed significantly higher MRCV titers than non-transmitting insects fed on single or mixed inocula, confirming that successful MRCV transmission is positively associated with viral accumulation in the insect. Furthermore, MRCV viral titers were higher in transmitting planthoppers that acquired this virus from a single inoculum than in those that acquired the virus from a mixed inoculum, indicating that the presence of the rhabdovirus somehow impaired MRCV replication and/or acquisition. This is the first study about interactions between MRCV and a rhabdovirus closely related to Maize yellow striate virus in this insect vector (D. kuscheli), and contributes to a better understanding of planthopper–virus interactions and their epidemiological implications.
Subirrigated pot-plant systems were developed for greenhouse simulations of field methods being tested for herbicidal control of saltcedar (Tamarix pentandra Pall.). The methods included: over-the-top spraying; soil injection; soil layering, with whole plants transplanted onto the herbicide layer; and soil layering by severing the roots and concomitantly injecting herbicide. Existing foliar application techniques were improved for greenhouse testing of herbicides and results from preliminary tests were apparently similar to literature reports of limited efficacy in the field. Preliminary results from these greenhouse techniques showed that root zone applications, either by soil layering or soil injection, were very effective; stem or foliar applications were less effective.