A novel entomopathogenic nematode (EPN) species, Steinernema tarimense n. sp., was isolated from soil samples collected in a Populus euphratica forest located in Yuli County within the Tarim Basin of Xinjiang, China. Integrated morphological and molecular analyses consistently place S. tarimense n. sp. within the ‘kushidai-clade’. The infective juvenile (IJ) of new species is characterized by a body length of 674–1010 μm, excretory pore located 53–80 μm from anterior end, nerve ring positioned 85–131 μm from anterior end, pharynx base situated 111–162 μm from anterior end, a tail length of 41–56 μm, and the ratios D% = 42.0–66.6, E% = 116.2–184.4, and H% = 25.5–45.1. The first-generation male of the new species is characterized by a curved spicule length of 61–89 μm, gubernaculum length of 41–58 μm, and ratios D% = 36.8–66.2, SW% = 117.0–206.1, and GS% = 54.8–82.0. Additionally, the tail of first-generation female is conoid with a minute mucron. Phylogenetic analyses of ITS, 28S, and mt12S sequences demonstrated that the three isolates of S. tarimense n. sp. are conspecific and form a sister clade to members of the ‘kushidai-clade’ including S. akhursti, S. anantnagense, S. kushidai, and S. populi. Notably, the IJs of the new species exhibited faster development at 25°C compared to other Steinernema species. This represents the first described of an indigenous EPN species from Xinjiang, suggesting its potential as a novel biocontrol agent against local pests.

The Edgerton crown is an iconic manifestation of drop impact splashing, with its prominent cylindrical edge decorated with detaching droplets. Herein, we identify the formation of an intriguing double-crown, when a high-viscosity drop impacts on a shallow pool of a lower-viscosity immiscible liquid. High-speed imaging shows that after the initial fine horizontal ejecta sheet, the first inner crown emerges vertically from the film liquid. This is followed by the second crown which forms near the outer base of the first crown, as the tip of the horizontally spreading viscous drop approaches the outer free surface. Axisymmetric numerical simulations, using the volume-of-fluid method with adaptive grid refinement, show that the flow squeezed out between the viscous drop and the solid surface, generates two counter-rotating vortex rings, which travel radially outwards together and drive out the second crown through the free surface. The bottom vortex emerges from the separated boundary layer at the solid wall, while the top one detaches from the underside of the viscous drop. We map out the narrow parameter regime, where this ephemeral structure emerges, in terms of viscosity ratio, impact velocity and film thickness.

Station-keeping control is a critical technology for stratospheric aerostats. For those aerostats that utilise wind field environments to achieve trajectory control, the station-keeping capability of a single aerostat is inherently limited. This limitation can lead to instances of the aerostat flying outside the designated task area, thereby diminishing the effectiveness of station-keeping control. To ensure continuous monitoring of the restricted area for long endurance, dynamic adjustments and cooperative coverage among multiple aerostats are necessary. This paper introduces an optimal coverage algorithm based on Voronoi diagrams and presents a formation control method for stratospheric aerostats that employs the virtual force method and the ${A^{\rm{*}}}$ algorithm, respectively. In a real wind field environment, ten aerostats are deployed to optimally cover the restricted area. Simulation results indicate that the coverage rate of the stratospheric aerostats within the restricted area can exceed 70%, while the network connectivity rate among the aerostats can reach 80% following guidance control during return flights. Furthermore, the stratospheric aerostats that flying out of the restricted area can return through path planning and optimal coverage algorithm, and the networking connectivity rate between aerostats is higher than that using the virtual force method.

This paper presents detailed analyses of the Reynolds stresses and their budgets in temporally evolving stratified wakes using direct numerical simulation. Ensemble averaging is employed to mitigate statistical errors in the data, and the results are presented as functions of both the transverse and vertical coordinates – at time instants across the near-wake, non-equilibrium, and quasi-two-dimensional regimes for wakes in weakly and strongly stratified environments. Key findings include the identification of dominant terms in the Reynolds stress transport equations and their spatial structures, the generation and destruction processes of the Reynolds stresses, and the energy transfer between the Reynolds stress and the mean flow. The study also clarifies the effects of the Reynolds number and the Froude number. Additionally, we assess the validity of the eddy-viscosity type models and some existing closures for the Reynolds stress model, highlighting the limitations of isotropy and return-to-isotropy hypotheses in stratified flows.

The depth-integrated horizontal momentum equations and continuity equation are employed to develop a new model. The vertical velocity and pressure can be expressed exactly in terms of horizontal velocities and free-surface elevation, which are the only unknowns in the model. Dividing the water column into elements and approximating horizontal velocities using linear shape function in each element, a set of model equations for horizontal velocities at element nodes is derived by adopting the weighted residual method. These model equations can be applied for transient or steady free-surface flows by prescribing appropriate lateral boundary conditions and initial conditions. Here, only the wave–current–bathymetry interaction problems are investigated. Theoretical analyses are conducted to examine various linear wave properties of the new models, which outperform the Green–Naghdi-type models for the range of water depth to wavelength ratios and the Boussinesq-type models as they are capable of simulating vertically sheared currents. One-dimensional horizontal numerical models, using a finite-difference method, are applied to a wide range of wave–current–bathymetry problems. Numerical validations are performed for nonlinear Stokes wave and bichromatic wave group propagation in deep water, sideband instability, regular wave transformation over a submerged shoal and focusing wave group interacting with linearly sheared currents in deep water. Very good agreements are observed between numerical results and laboratory data. Lastly, numerical experiments of wave shoaling from deep to shallow water are conducted to further demonstrate the capability of the new model.

We report direct numerical simulations results of the rough-wall channel, focusing on roughness with high $k_{rms}/k_a$ statistics but small to negative $Sk$ statistics, and we study the implications of this new dataset on rough-wall modelling. Here, $k_{rms}$ is the root mean square, $k_a$ is the first-order moment of roughness height, and $Sk$ is the skewness. The effects of packing density, skewness and arrangement of roughness elements on mean streamwise velocity, equivalent roughness height ($z_0$) and Reynolds and dispersive stresses have been studied. We demonstrate that two-point correlation lengths of roughness height statistics play an important role in characterizing rough surfaces with identical moments of roughness height but different arrangements of roughness elements. Analysis of the present as well as historical data suggests that the task of rough-wall modelling is to identify geometric parameters that distinguish the rough surfaces within the calibration dataset. We demonstrate a novel feature selection procedure to determine these parameters. Further, since there is no finite set of roughness statistics that distinguish between all rough surfaces, we argue that obtaining a universal rough-wall model for making equivalent sand-grain roughness ($k_s$) predictions would be challenging, and that each rough-wall model would have its applicable range. This motivates the development of group-based rough-wall models. The applicability of multi-variate polynomial regression and feedforward neural networks for building such group-based rough-wall models using the selected features has been shown.

The cyst nematodes, subfamily Heteroderinae, are plant pathogens of worldwide economic significance. A new cyst nematode of the genus Cactodera within the Heteroderinae, Cactodera xinanensis n. sp., was isolated from rhizospheres of crops in the Guizhou and Sichuan provinces of southwest China. The new species was characterized by having the cyst with a length/width = 1.3 ± 0.1 (1.1–1.6), a fenestral diameter of 28.1 ± 4.3 (21.3–38.7) μm, vulval denticles present; second-stage juvenile with stylet 21.5 ± 0.5 (20.3–22.6) μm long, tail 59.4 ± 2.0 (55.9–63.8) μm long and hyaline region 28.7 ± 2.7 (25.0–36.3) μm long, lateral field with four incisures; the eggshell with punctations. The new species can be differentiated from other species of Cactodera by a longer tail and hyaline region of second-stage juveniles. Phylogenetic relationships within populations and species of Cactodera are given based on the analysis of the internal transcribed spacer (ITS-rRNA), the large subunit of the nuclear ribosomal RNA (28S-rRNA) D2-D3 region and the partial cytochrome oxidase subunit I (COI) gene sequences here. The ITS-rRNA, 28S-rRNA and COI gene sequences clearly differentiated Cactodera xinanensis n. sp. from other species of Cactodera. A key and a morphological identification characteristic table for the species of Cactodera are included in the study.

Idioms play an important role in language; however, little research has examined idioms in children’s natural language settings. This study explored idioms usage in maternal talk during mother-child shared book reading and its relation to children’s vocabulary development. Thirty-three Chinese children in Norway (aged 3;0–5;5) and their mothers participated. We observed shared reading at the onset of the study and assessed children’s receptive and expressive vocabulary in Chinese three times across one year. Results demonstrated that mothers used an average of 1.8 idioms and explained one-third of the idioms. Maternal idiom usage was correlated with their talk amount and lexical diversity. Individual growth modeling revealed that the number of idioms mothers used predicted the growth of children’s receptive vocabulary in Chinese. We speculate that idiom usage could be an effective and understudied marker of parental linguistic sophistication. This study underscores the importance of idiom exposure in children’s language environment.

OBJECTIVES/GOALS: Incomplete mucosal healingand dysbiosis prevent long-term remission after colitis. IL4 may restore colon homeostasis through its action on immune cells and the microbiome. We will demonstrate this mechanism using genetically modified mice and molecular tools. This may result in target therapies that prolong remission in patients with IBD. METHODS/STUDY POPULATION: Mice were treated with 3% dextran sulfate sodium (DSS) in drinking water for 5 days to induce colitis. Mice were monitored daily for changes in body weight, and to monitor colitis severity. At each endpoint, mice were sacrificed and colon length was measured. For disease severity assessment, mouse colons were prepared in paraffin sections by the 'swiss-rolling' method. For flow cytometry, lamina propria mononuclear cell isolation was performed and cellular populations were stained with fluorophore-conjugated antibodies. IL4-eGFP-expressing (4get) mice were used to analyze the cellular expression of IL4 after colitis. Cell-specific IL4 deletion mice were generated using the cre-lox system. RESULTS/ANTICIPATED RESULTS: IL4-deficient mice had worse colitis compared with wild-type controls. Flow cytometry of lamina propria cells from 4get mice showed that most IL4-producing cells after colitis are eosinophils (CD11b+SiglecF+). Flow cytometry of C57bl6 mice showed an influx of IL4Ra+ monocytes (CD11b+Ly6C+) and macrophages (CD11b+F480+). IL4-stimulated bone marrow-derived macrophages demonstrated an increase in HB-EGF mRNA transcription. Myeloid-specific IL4R deleted mice had no difference in colitis severity compared with controls. Neutrophil-specific IL4R-deleted mice had increased colitis severity and mortality. Co-housing of littermate mice rescued recovery after DSS in IL4 deficient mice. DISCUSSION/SIGNIFICANCE: IL4 appears to play a role in restoring homeostasis after colitis. The mechanism depends on eosinophil-derived IL4, and action through neutrophils. However, the reparative function of IL4 can be shared with deficient mice through the microbiome. I will study the cellular and microbial mechanism by which IL4 restores homeostasis after colitis.

Amplification of velocity gradients, a key feature of turbulent flows, is affected by the non-local character of the incompressible fluid equations expressed by the second derivative (Hessian) of the pressure field. By analysing the structure of the flow in regions where the vorticity is the highest, we propose an approximate expression for the pressure Hessian in terms of the local vorticity, consistent with the existence of intense vortex tubes. Contrary to the often used simplification of an isotropic form for the pressure Hessian, which in effect inhibits vortex stretching, the proposed approximate form of the pressure Hessian enables much stronger vortex stretching. The prediction of the approximation proposed here is validated with results of direct numerical simulations of turbulent flows.

Direct numerical simulations are conducted for temporally evolving stratified wake flows at Reynolds numbers from $10\,000$ to $50\,000$ and Froude numbers from $2$ to 50. Unlike previous studies that obtained statistics from a single realization, we take ensemble averages among 80–100 realizations. Our analysis shows that data from one realization incur large convergence errors. These errors reduce quickly as the number of statistical samples increases, with the benefit of ensemble average diminishing beyond 40–60 realizations. The data with ensemble average allow us to test the previously established scalings and arrive at new scaling estimates. Specifically, the data do not support power-law scaling in the centreline velocity deficit $U_0$ beyond the near wake. Its decay rate increases continuously from 0.1 at the onset of the non-equilibrium regime until the end of our calculations without reaching any asymptote. Additionally, while no power-law scalings could be found in the wake width ($L_H$) and wake height ($L_V$) in the late wake, $L_H\sim (Nt)^{1/3}$ is a good working approximation of the wake's horizontal size, where $N$ is buoyancy frequency and $t$ is time. Besides the low-order statistics, we also report the transverse integrated terms and the vertically integrated terms in the turbulent kinetic energy budget equation as a function of the vertical and transverse coordinates. The data indicate that there are two peaks in the vertically integrated production and transport terms, and one peak when the two terms are integrated horizontally.

To accelerate high-intensity heavy-ion beams to high energy in the booster ring (BRing) at the High-Intensity Heavy-Ion Accelerator Facility (HIAF) project, we take the typical reference particle 238U35+, which can be accelerated from an injection energy of 17 MeV/u to the maximal extraction energy of 830 MeV/u, as an example to study the basic processes of longitudinal beam dynamics, including beam capture, acceleration, and bunch merging. The voltage amplitude, the synchronous phase, and the frequency program of the RF system during the operational cycle were given, and the beam properties such as bunch length, momentum spread, longitudinal beam emittance, and beam loss were derived, firstly. Then, the beam properties under different voltage amplitude and synchronous phase errors were also studied, and the results were compared with the cases without any errors. Next, the beam properties with the injection energy fluctuation were also studied. The tolerances of the RF errors and injection energy fluctuation were dictated based on the CISP simulations. Finally, the effect of space charge at the low injection energy with different beam intensities on longitudinal emittance and beam loss was evaluated.

Compact obscured nuclei (CONs) are relatively common in the centers of local (U)LIRGs, yet their nature remains unknown. Both AGN activity and extreme nuclear starbursts have been suggested as plausible nuclear power sources. The prevalence of outflows in these systems suggest that CONs represent a key phase in the nuclear feedback cycle, in which material is ejected from the central regions of the galaxy. Here, we present results from MUSE for the confirmed local CON galaxy NGC4418. For the first time we spatially map the spectral features and kinematics of the galaxy in the optical, revealing several previously unknown structures. In particular, we discover a bilateral outflow along the minor axis, an outflowing bubble, several knot structures and a receding outflow partially obscured by the galactic disk. Based on the properties of these features, we conclude that the CON in NGC4418 is most likely powered by an AGN.