Fall- and spring-seeded cover crops are commonly used by organic no-till practitioners, but spring-seeded mixtures have not been as widely studied, particularly regarding their management under short growing periods in cool-climate regions. This study evaluated the short-term effects of spring-seeded cover crop termination methods and organic fertilization rates on subsequent crop yields and soil mineral nitrogen (N) dynamics on a clay loam soil in Quebec, Canada. In both years (2022–2023), a spring-seeded cover crop mixture was terminated in mid-summer, and then a vegetable crop was transplanted and fertilized at three different rates. Cover crop termination methods consisted of roller-crimping (ROLL), flail mowing and tarping (TARP), flail mowing and disking (DISK), and a fallow control without cover crops (CTRL). The combination of pelletized composted poultry manure and blood meal was applied at three rates (0% N, 50% N, and 100% N) during the production of broccoli (Brassica oleracea var. italica) in 2022 and beetroot (Beta vulgaris var. esculenta) in 2023. In 2022, field peas (Pisum sativum L.)-oats (Avena sativa L.) produced an average of 2.9 Mg DM ha−1, whereas the aboveground biomass of field peas-faba beans (Vicia faba L.) in 2023 yielded about 2.3 Mg DM ha−1. Flail mowing and tarping (TARP) increased total broccoli and beetroot yields by 38% and 44%, respectively, compared to roller-crimping, likely due to N availability rather than weed competition. Soil mineral N after tarp removal in 2023 was up to twice as great in the fully fertilized TARP treatment compared to other treatments. Vegetable N uptake, particularly in broccoli, was the lowest in the ROLL treatment, indicating a strong connection between plant N uptake and yields. Termination was performed at a phenological stage that was too early, resulting in low effectiveness of the roller-crimper. Interestingly, the TARP and DISK treatments produced comparable yields. Overall, cover crops could not compensate for reduced fertilizer inputs, which is further supported by the lack of termination method × fertilization rate interactions. Limited cover crop biomass production due to the short growing period resulted in minimal yield differences between treatments with cover crops and the CTRL treatment. Although this study was limited to a single field in Quebec, it shows that tarping may be a better option for organic vegetable growers, as it facilitates the termination of cover crops without tillage compared to roller-crimping.

Bioregenerative life support systems (BLSS) designed to produce food crops in future crewed missions to the Moon or Mars consider in-situ resource utilisation (ISRU). Lunar regolith is, therefore, in focus for future technologies of farming on lunar bases. We tested germination and early growth of broccoli plants (Brassica oleracea var. botrytis italica) in Murashige-Skoog liquid medium with addition of leachate from a lunar regolith simulant. As the additions to growth, differently diluted water and acidic leachates were used. Physiological status of the germinating plants was evaluated by chlorophyll fluorescence parameters related to plant vitality (relative fluorescence decline – Rfd) and photosynthetic performance of photosystem II (1) potential (FV/FM) and (2) effective quantum yield of PSII (ΦPSII), photochemical quenching referring to number of open PSII reaction centres. Both water and acidic leachates inhibited plant growth, however, the extent of growth limitation was dilution-dependent. Full inhibition of germination was apparent when undiluted acidic leachate was added. However, 50% dilution (and higher) resulted in seed germination and the early growth. No negative effects of the water dilutions on FV/FM as well as ΦPSII, were apparent in 15 days old plants, their cotyledonary and the first primary leaves, in particular. Similarly, qP and Rfd showed no sign of either water or acidic leachate addition effect. Although photosystem II-related parameters exhibited no negative effect of the leachates addition, a growth of plants was found dilution-dependent: higher degree of dilution resulted in a more pronounced reduction in plant projection area. In spite of the growth rate reduction (compared to untreated control), properly diluted water and acidic leachates from lunar regolith and/or its simulants might be used in follow up studies focused on plant species prospective for future cultivation in Moon-based stations with temporary or permanent crew.

In 2021 and 2022, research was initiated at two locations to evaluate the efficacy and safety of sulfentrazone in transplanted cabbage and broccoli. Treatments included oxyfluorfen at 560 g ha−1 applied pretransplant (PRE-T), sulfentrazone applied at 116 or 233 g ha−1 PRE-T, and S-metolachlor applied at 715 g ha−1 immediately after transplanting (POST-T) followed by (fb) oxyfluorfen applied at 210 g ha−1 postemergence (POST) 14 d after planting (DAP). The weedy cover of nontreated plots averaged between 6% (14 DAP) and 72% (42 DAP); all herbicide-treated plots averaged less than 30% cover at 42 DAP. At 14 and 28 DAP, oxyfluorfen, S–metolachlor fb oxyfluorfen, and the high rate of sulfentrazone reduced total monocotyledonous and dicotyledonous weed densities by 62% and 100%, respectively, relative to the nontreated control. The density of hairy galinsoga (in New Jersey) and combined ladysthumb and prostrate knotweed (in New York) were reduced by 71% to 99%. Except for the low rate of sulfentrazone, all herbicide treatments reduced weed biomass at harvest by ≥88%. Crop injury varied in response to herbicide treatments or weed competition but was also affected by crop and location. Between 14 and 28 DAP, the greatest amount of stunting (22%) was noted in the S-metolachlor fb oxyfluorfen treatments at both locations. Averaged over treatments, greater stunting was observed in broccoli than in cabbage in New York, whereas stunting estimates were higher for cabbage in New Jersey. All treatments in New Jersey resulted in significantly increased cabbage yield and broccoli and cabbage head sizes relative to the nontreated controls. No yield difference was noted between herbicide treatments and the nontreated check in New York. Data derived from these studies will be used to enhance crop safety recommendations in northeastern U.S. production environments for sulfentrazone used PRE in transplanted cabbage and support a potential label for broccoli.

Cole crops including broccoli and collard contribute more than $119 million to Georgia’s farm gate value yearly. To ensure maximum profitability, these crops must be planted into weed-free fields. Glyphosate is a tool often used to help achieve this goal because of its broad-spectrum activity on weeds coupled with the knowledge that it poses no threat to the succeeding crop when used as directed. However, recent research suggests that with certain soil textures and production systems, the residual soil activity of glyphosate may damage some crops. Therefore, field experiments were conducted in fall 2019 and 2020 to evaluate transplanted broccoli and collard response to glyphosate applied preplant onto bare soil and what practical mitigation measures could be implemented to reduce crop injury. Herbicide treatments consisted oGf 0, 2.5, or 5 kg ae ha−1 glyphosate applied preplant followed by 1) no mitigation measure, 2) tillage, 3) irrigation, or 4) tillage and irrigation prior to transplanting broccoli and collard by hand. When no mitigation was implemented, the residual activity of glyphosate at 2.5 and 5.0 kg ae ha−1 resulted in 43% to 71% and 79% to 93% injury to broccoli and collard transplants, respectively. This resulted in a 35% to 50% reduction in broccoli marketable head weights and 63% to 71% reduction in collard leaf weights. Irrigation reduced visible damage by 28% to 48%, whereas tillage reduced injury by 43% to 76%, for both crops. Irrigation alleviated yield losses for broccoli but only tillage eliminated yield loss for both crops. Care must be taken when transplanting broccoli and collard into a field recently treated with glyphosate at rates ≥2.5 kg ae ha−1. Its residual activity can damage transplants with injury levels influenced by glyphosate rate, and tillage or irrigation after application and prior to planting.

A large and growing body of literature has studied consumer willingness to pay (WTP) for local foods in the United States. However, these studies implicitly assume that consumers perceive local foods to have superior quality than nonlocal foods. Little is known about WTP for local foods when taking into account differences in consumer perception of food quality between local and nonlocal foods. In this article, we conduct an economic experiment to assess the effect of locally grown information on consumer WTP and quality perceptions of three broccoli varieties (one commercial variety grown in California and two newly developed local varieties). Our results show that consumers rate both the appearance and the taste of the two local broccoli varieties lower than the California variety when evaluating food quality blindly. However, consumers’ evaluations of the two local varieties improve substantially after being told the two varieties are locally grown. Results also indicate that consumers are willing to pay a price premium for the two local varieties after being told that they are locally grown. Our results provide evidence that locally grown information has a positive effect on both consumer WTP and quality perception of local foods.

Sulphoraphane originates from glucoraphanin in broccoli and is associated with anti-cancer effects. A preclinical study suggested that daily consumption of broccoli may increase the production of sulphoraphane and sulphoraphane metabolites available for absorption. The objective of this study was to determine whether daily broccoli consumption alters the absorption and metabolism of isothiocyanates derived from broccoli glucosinolates. We conducted a randomised cross-over human study (n 18) balanced for BMI and glutathione S-transferase μ 1 (GSTM1) genotype in which subjects consumed a control diet with no broccoli (NB) for 16 d or the same diet with 200 g of cooked broccoli and 20 g of raw daikon radish daily for 15 d (daily broccoli, DB) and 100 g of broccoli and 10 g of daikon radish on day 16. On day 17, all subjects consumed a meal of 200 g of broccoli and 20 g of daikon radish. Plasma and urine were collected for 24 h and analysed for sulphoraphane and metabolites of sulphoraphane and erucin by triple quadrupole tandem MS. For subjects with BMI >26 kg/m2 (median), plasma AUC and urinary excretion rates of total metabolites were higher on the NB diet than on the DB diet, whereas for subjects with BMI <26 kg/m2, plasma AUC and urinary excretion rates were higher on the DB diet than on the NB diet. Daily consumption of broccoli interacted with BMI but not GSTM1 genotype to affect plasma concentrations and urinary excretion of glucosinolate-derived compounds believed to confer protection against cancer. This trial was registered as NCT02346812.

Machine-vision cultivator guidance systems are commercially available to growers, but little work has been done to determine if these guidance systems can improve integrated weed management systems in vegetable crops. Studies were conducted in 2005 and 2006 in broccoli and lettuce to evaluate band-applied DCPA or pronamide, respectively, and four noncultivated bands ranging from 5.1 to 12.7 cm. DCPA or pronamide were applied in bands centered on the seed line at 0, 7.6 or 12.7 cm wide. A commercial machine-vision system was used to guide a commercial cultivator. Generally, weed densities and hand-weeding times were less where the DCPA band in broccoli or the pronamide band in lettuce were 7.6 or 12.7 cm wide compared to no herbicide. Weed densities were lowest in both crops where the noncultivated band width was 5.1 cm compared to 12.7-cm noncultivated bands. For broccoli in both 2005 and 2006, net returns above production costs were generally higher in the 7.6- and 12.7-cm-wide DCPA bands compared with the no-herbicide band. In lettuce in both years, the no-pronamide treatment had higher net returns, when compared with the 7.6- and 12.7-cm pronamide bands. Lettuce yields and higher net returns in the no-pronamide treatment compared to the 7.6- and 12.7-cm pronamide bands may be due to slight yield reduction from pronamide. Results suggest that pronamide was not needed during the dry months of the year when weed management tools such as hand-weeding and cultivation work very well. However, in periods of rainy weather when cultivation and hand-weeding are not possible, then pronamide would likely provide the greatest economic benefit. Given the large impact of cultivation on vegetable weed management programs, the greatest potential benefit of machine-vision guided cultivators is if they facilitate more timely and effective cultivation.

The performance of the Robovator (F. Poulsen Engineering ApS, Hvals⊘, Denmark), a commercial robotic intrarow cultivator, was evaluated in direct-seeded broccoli and transplanted lettuce during 2014 and 2015 in Salinas, CA, and Yuma, AZ. The main objective was to evaluate the crop stand after cultivation, crop yield, and weed control efficacy of the Robovator compared with a standard cultivator. A second objective was to compare hand weeding time after cultivation within a complete integrated weed management (IWM) system. Herbicides were included as a component of the IWM system. The Robovator did not reduce crop stand or marketable yield compared with the standard cultivator. The Robovator removed 18 to 41% more weeds at moderate to high weed densities and reduced hand-weeding times by 20 to 45% compared with the standard cultivator. At low weed densities there was little difference between the cultivators in terms of weed control and hand-weeding times. The lower-hand weeding time with the Robovator treatments suggest that robotic intrarow cultivators can reduce dependency on hand weeding compared with standard cultivators. Technological advancements and price reductions of these types of machines will likely improve their weed removal efficacy and the long-term viability of IWM programs that will use them.

A primary challenge of managing vegetable production on a small land base is the maintenance and building of soil quality. Previous studies have demonstrated the benefits of cover crops for improved soil quality; however, small growers struggle to fit cover crops into rotations. Small-scale growers with limited available land are under significant pressure to maximize their saleable yield and often work to maximize output by using intensive cropping practices that may include both early and late season crops, thus limiting the typical shoulder season windows in which cover crops can be grown. In-season living mulches may be an effective strategy to provide small-scale growers the benefits of cover crops with less land commitment than cover crops used in typical rotations. However, research on living mulches is generally not suited to small-scale organic production systems due to the typical reliance on chemical herbicide to suppress mulches. An experiment was designed with the goal of evaluating living mulch systems for space-limited organic vegetable production. In a 2-year study, four living mulch crops (buckwheat (Fagopyrum esculentum), field pea (Pisum sativum), crimson clover (Trifolium incarnatum) and medium red clover (Trifolium pratense)) and a cultivated control with no mulch cover were planted in early spring each year. Snap beans (Phaseolus vulgaris var. Tavera), transplanted bell peppers (Capsicum annuum var. Revolution), and transplanted fall broccoli (Brassica oleracea var. Imperial) were then planted directly into living mulches. During each summer growing season, living mulches and weeds were mown between-rows and hand-weeded in-row approximately every 10–14 days as needed for management. Labor times for mowing and cultivation were found to be higher in all treatments relative to the cultivated control. An inverse relationship between living mulch biomass and weed biomass was observed, demonstrating that living mulches may contribute to weed suppression. However, lower vegetable yields were seen in the living mulch treatments, most likely due to resource competition among vegetables, living mulches and weeds. High pre-existing weed seedbank and drought conditions likely increased competition and contributed to reduced vegetable yield.

Sulforaphane (SF) is a chemopreventive isothiocyanate (ITC) derived from glucoraphanin (GRP) hydrolysis by myrosinase, a thioglucoside present in broccoli. The ability of broccoli powders sold as supplements to provide dietary SF is often of concern as many supplements contain GRP, but lack myrosinase. In a previous study, biomarkers of SF bioavailability from a powder rich in GRP, but lacking myrosinase, were enhanced by co-consumption of a myrosinase-containing air-dried broccoli sprout powder. Here, we studied the absorption of SF from the GRP-rich powder used in the previous study, but in combination with fresh broccoli sprouts, which are commercially available and more applicable to the human diet than air-dried sprout powder. A total of four participants each consumed four meals (separated by 1 week) consisting of dry cereal and yogurt with sprouts equivalent to 70 μmol SF, GRP powder equivalent to 120 μmol SF, both or neither. Metabolites of SF were analysed in blood and urine. The 24 h urinary SF-N-acetylcysteine recovery was 65, 60 and 24 % of the dose ingested from combination, broccoli sprout and GRP powder meals, respectively. In urine and plasma, ITC appearance was delayed following the GRP powder meal compared with the sprout and combination meals. Compared with the GRP powder or sprouts alone, combining broccoli sprouts with the GRP powder synergistically enhanced the early appearance of SF, offering insight into the combination of foods for improved health benefits of foods that reduce the risk for cancer.

Recently, broccoli, a vegetable of the Brassica family, has been found to protect the myocardium from ischaemic reperfusion injury through the redox signalling of sulphoraphane, which is being formed from glucosinolate present in this vegetable. Since cooked broccoli loses most of its glucosinolate, we assumed that fresh broccoli could be a superior cardioprotective agent compared to cooked broccoli. To test this, two groups of rats were fed with fresh (steamed) broccoli or cooked broccoli for 30 d, while a third group was given vehicle only for the same period of time. After 30 d, all the rats were sacrificed, and the isolated working hearts were subjected to 30 min ischaemia followed by 2 h of reperfusion. Both cooked and steamed broccolis displayed significantly improved post-ischaemic ventricular function and reduced myocardial infarction and cardiomyocyte apoptosis compared to control, but steamed broccoli showed superior cardioprotective abilities compared with the cooked broccoli. Corroborating with these results, both cooked and steamed broccolis demonstrated significantly enhanced induction of the survival signalling proteins including Bcl2, Akt, extracellular signal-regulated kinase 1/2, haemoxygenase-1, NFE2 related factor 2, superoxide dismutase (SOD1) and SOD2 and down-regulation of the proteins (e.g. Bax, c-Jun N-terminal kinase, p38 mitogen-activated protein kinase) of the death signalling pathway, steamed broccoli displaying superior results over its cooked counterpart. The expressions of proteins of the thioredoxin (Trx) superfamily including Trx1 and its precursor sulphoraphane, Trx2 and Trx reductase, were enhanced only in the steamed broccoli group. The results of the present study documented superior cardioprotective properties of the steamed broccoli over cooked broccoli because of the ability of fresh broccoli to perform redox signalling of Trx.

Sulforaphane is a naturally occurring isothiocyanate with promising chemopreventive activity. An analytical method, utilising liquid chromatography-MS/MS, which allows the determination of sulforaphane in small volumes of rat plasma following exposure to low dietary doses, was developed and validated, and employed to determine its absolute bioavailability and pharmacokinetic characteristics. Rats were treated with either a single intravenous dose of sulforaphane (2·8 μmol/kg) or single oral doses of 2·8, 5·6 and 28 μmol/kg. Sulforaphane plasma concentrations were determined in blood samples withdrawn from the rat tail at regular time intervals. Following intravenous administration, the plasma profile of sulforaphane was best described by a two-compartment pharmacokinetic model, with a prolonged terminal phase. Sulforaphane was very well and rapidly absorbed and displayed an absolute bioavailability of 82 %, which, however, decreased at the higher doses, indicating a dose-dependent pharmacokinetic behaviour; similarly, Cmax values did not rise proportionately to the dose. At the highest dose used, the rate of absorption constant kab, biological half-life t½ and apparent volume of distribution decreased significantly. It is concluded that in the rat orally administered sulforaphane is rapidly absorbed, achieving high absolute bioavailability at low dietary doses, but dose-dependent pharmacokinetics was evident, with bioavailability decreasing with increasing dose.

Cruciferous vegetables have been studied extensively for their chemoprotective effects. Although they contain many bioactive compounds, the anti-carcinogenic actions of cruciferous vegetables are commonly attributed to their content of glucosinolates. Glucosinolates are relatively biologically inert but can be hydrolysed to a range of bioactive compounds such as isothiocyanates (ITC) and indoles by the plant-based enzyme myrosinase, or less efficiently by the colonic microflora. A number of mechanisms whereby ITC and indoles may protect against colo-rectal cancer have been identified. In experimental animals cruciferous vegetables have been shown to inhibit chemically-induced colon cancer. However, the results of recent epidemiological cohort studies have been inconsistent and this disparity may reflect a lack of sensitivity of such studies. Possible explanations for the failure of epidemiological studies to detect an effect include: assessment of cruciferous vegetable intake by methods that are subject to large measurement errors; the interaction between diet and genotype has not been considered: the effect that post-harvest treatments may have on biological effects of cruciferous vegetables has not been taken into account.

Phosphorus (P) accumulation and use efficiency by broccoli (Brassica oleraceae var. italica) were observed in a field plot study comparing management with varying levels of conventional fertilizer, differing composted amendments, and a combination of inorganic Nitrogen (N) and compost sources. Total P accumulation and yield were highest in treatments incorporating solely compost or vermicompost applications of 30 tons ha–1. Applications of N as ammonium sulfate caused significant soil acidification, which lowered yield and P uptake. Poor growth and yield were probably related to manganese toxicity effects on root extension and activity. These effects were partly ameliorated in treatments combining fertilizer N and compost sources. As a result, yield in plots receiving 75 kg N ha–1 as ammonium sulfate and 8.9 kg P ha–1 as compost was nearly identical to highest experimental yields. Phosphorus use efficiency was highest in this treatment incorporating fertilizer N and compost. Generally, P use efficiency tended to decline at the highest levels of fertilization. We propose that intensive organic matter management may be a good way to improve P use efficiency by vegetable crops.

The market value for organically grown produce in California was estimated to be at least 54 to 68 million dollars at wholesale prices in 1987. This market is rapidly growing. A 41 percent increase in the value of organically grown produce was observed in 1987. When this analysis was completed in early 1988, it was estimated that the wholesale value of the market could exceed $300 million by 1992. A sample analysis of 1986–1987 price and volume for organically grown broccoli showed relatively steady prices and a volume increase of over 50 percent, indicating a similar growth in market demand. Prices for organically grown produce are significantly higher than for conventional produce; however, in spite of high weekly volume volatility, organic prices appear to be less volatile than prices for conventional produce. This paper proposes that the collection, reporting, and analysis of organic produce market data be continued and improved and that new sources of data be integrated to ensure continued availability of timely market information. Availability of more market information should increase efficiency and aid the development of the organic produce industry. The paper also proposes that overall trends in the organic produce industry be systematically monitored by Federal-State Market News and that the increased use of market information by organic growers, growers considering the transition, and other interested parties in the industry be promoted.