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The cell cycle is a complex biological phenomenon, which plays an important role in many cell biological processes and disease states. Machine learning is emerging to be a pivotal technique for the study of the cell cycle, resulting in a number of available tools and models for the analysis of the cell cycle. Most, however, heavily rely on expert annotations, prior knowledge of mechanisms, and imaging with several fluorescent markers to train their models. Many are also limited to processing only the spatial information in the cell images. In this work, we describe a different approach based on representation learning to construct a manifold of the cell life cycle. We trained our model such that the representations are learned without exhaustive annotations nor assumptions. Moreover, our model uses microscopy images derived from a single fluorescence channel and utilizes both the spatial and temporal information in these images. We show that even with fewer channels and self-supervision, information relevant to cell cycle analysis such as staging and estimation of cycle duration can still be extracted, which demonstrates the potential of our approach to aid future cell cycle studies and in discovery cell biology to probe and understand novel dynamic systems.
This chapter follows up on some of the observations made in the introduction to this volume where we briefly reflect on contributions to theoretical approaches notably by eco-feminists. The chapter focuses very specifically on these latter contributions and in the following pages I seek to advance and develop our theoretical and conceptual understanding of green criminology using eco-feminism as the springboard and benchmark for assessing the extent to which green criminology is gendered and for developing a framework for embedding a gendered approach to the green criminological and victimological project. Part of the task that is set for this chapter, therefore, is to help with the review of determining what has been achieved so far, and part is about stepping up and forward to thoroughly gender the green criminological project drawing on appropriate theoretical traditions and trajectories.
Ecological (eco-)feminism holds out for a gender-sensitive form of justice into the 21st century. In lay terms, eco-feminism is a feminist approach to analysing the relationships between humans and the natural world. The foundation of eco-feminism is the relationship between women, the earth and environmentalism. If we take this as the starting point, we can begin to ask some important questions about green criminology. A modern definition of eco-justice is one that encompasses environmental justice (relating to humans and their relationship with specific environments), ecological justice (referring primarily to the health and wellbeing of eco-systems) and species justice (involving rights and biodiversity concerns as these pertain to nonhuman animals and plants) (White, 2013). This definition is also a useful starting point, and conjoining this with our interest in exploring ecofeminism we can illuminate some important features about the state of green criminology knowledge more broadly. For example, we can expose what we know and do not know about who and what perpetrates crime and harm and who and what can be and are victims. The latter thread – the victimological – has been especially underdeveloped and until very recently knowledge of the gendered nature of victimisation has been scant. Broader questions about the nature and extent of victimisation have, however, surfaced such that scholars are now proposing that victimisation extends beyond humans to nonhuman species, that inanimate objects can be a victim, that multi-and poly-species victimisation is experienced.
We investigate the statistics of turbulence in emulsions of two immiscible fluids of the same density. We compute velocity increments between points conditioned to be located in the same phase or in different phases, and examine their probability density functions (PDFs) and the associated structure functions (SFs). This enables us to demonstrate that the presence of the interface reduces the skewness of the PDF at small scales and therefore the magnitude of the energy flux towards the dissipative scales, which is quantified by the third-order SF. The analysis of the higher-order SFs shows that multiphase turbulence is more intermittent than single-phase turbulence. In particular, the local scaling exponents of the SFs display a saturation below the Kolmogorov–Hinze scale, which indicates the presence of large velocity gradients across the interface. Interestingly, the statistics of the velocity differences in the carrier phase recovers that of single-phase turbulence when the viscosity of the dispersed phase is high.
A mathematical and numerical framework is proposed to compute the displacement and merging dynamics of sliding droplets under the action of a constant shear exerted by a gas flow. An augmented formulation is implemented to model surface tension including the full curvature of the free surface. A set of shallow-water evolution equations is obtained for the film thickness, the averaged velocity, an additional quantity (with dimension of a velocity) taking into account the capillary effects and a tensor called enstrophy. The enstrophy accounts for the deviation of the velocity profile from a constant velocity distribution. The formulation is consistent with the long-wave expansion of the basic equations with a conservative part and source terms including the effect of viscosity, in the form of a viscous friction and the effect of the shear stress. The model is hyperbolic with generalised diffusion terms due to capillarity. Finally, our model is completed with a disjoining pressure formulation that is able to account for the hysteresis of the static contact angle. In this formulation, the advancing or receding nature of the contact line is assessed by the accumulation or reduction of mass of the droplet at the contact line. Simulations of sliding water droplets are performed with periodic boundary conditions in a domain of limited size. Hysteresis of the static contact angle causes a slowdown of the drops and a delay in the sequence of coalescence of the drops.
This is the first book that systemically introduces the theory and implementation of Computational Grains for micromechanical modeling of heterogeneous materials. This book covers the specifically designed mathematics embedded in Computational Grains, and the entire process of microstructure construction, tessellation, CG simulation and homogenization. The Computational Grains discussed in this book consider elastic, non-elastic, and multi-physics solids. Materials damage development are also preliminarily discussed, with CGs considering matrix-inclusion debonding as well as embedded microcracks. Presenting the theory step-by-step and with detailed examples and MATLAB codes, the material is accessible and practical for readers. This will be ideal for graduate students and researchers in mechanical and aerospace engineering and applied mechanics.
Multiphase flow is an area of fluid dynamics that describes interactions between two or more phases of matter and is relevant across a wide range of industrial processes and natural environmental systems, from the transport of natural resources to volcanic ash flow. This book covers the topic in detail, providing clear explanations of the underlying physics behind the complex behaviour of solid particles in fluids. The forces involved in particle-fluid interactions are first used to describe the interactions between the particles, and the fundamentals of contact mechanics are then outlined and applied to model interparticle collisions. The book is illustrated with frequent worked examples and algorithms, enabling the reader to develop the required tools for simulating the flow of fluids with solid particles. This self-contained text will appeal to physicists, applied mathematicians and mechanical engineers working in this important area of research.
A wideband antipodal Vivaldi antenna has been designed and optimized. A slight improvement is obtained by employing multiple metalense based on circular split-ring resonators to maximize the antenna gain with the maximum bandwidth. The designed antennas have been fabricated and characterized, showing good agreement with simulations. The maximum measured gain is $G = 12\;{\textrm{dB}}$, and the −10 dB bandwidth is from $f = 3\;{\textrm{GHz}}$ to $f = 13\;{\textrm{GHz}}$.
This paper proposes a wideband four-element multiple-input multiple-output (MIMO) antenna operating in the millimeter-wave frequency band for 5G communications from 24.37–39.44 and 45.09–50.62 GHz. The antenna design has been realized using Rogers 5880 substrate and consists of a T-shaped strip that is attached to the top of a 50 Ω feeding line. Two microstrip lines are affixed at the terminal points of T-shaped strip and bent toward the feeding structure. These bent strip lines are then extended by joining two additional L-shaped microstrip lines. On the back, there is a partial defective ground plane featuring a rectangular slot and a thin strip line located at its center. The dimensions of the proposed single element and MIMO antenna are 0.933λ × 0.933λ × 0.024λ and 1.865λ × 1.865λ × 0.024λ, respectively (at 28 GHz), while the edge-to-edge distance between the radiation elements of the MIMO antenna is 4.0 mm. The design incorporates an efficient passive fan-shaped decoupling structure to decrease coupling between antenna elements. Simulations and experimental results show good agreement with each other. For all resonant frequencies the measured peak gain is greater than 4.85 dBi, radiation efficiency is over 90%, diversity gain is greater than 9.5, ECC is less than 0.025, TARC is less than -10 dB and CCL is below 0.4 bits/s/Hz. The proposed MIMO antenna, with its wide bandwidth, high gain, high inter-port isolation, and high efficiency characteristics, can be used for 5G wireless communication applications.
The large-eddy simulation technique was used to investigate the dynamics of unsteady flow separation on a flat-plate turbulent boundary layer. The unsteadiness was generated by imposing an oscillating, wall-normal velocity profile at the top of the computational domain, and a range of reduced frequencies ($k$), from a very rapid flutter-like motion to a slow quasi-steady oscillation, was studied. Ambrogi et al. (J. Fluid Mech., vol. 945, 2022, A10) showed that the reduced frequency greatly affects the transient separation process, and at a frequency $k=1$, the separation region became unstable and was advected periodically out of the domain. In this paper, we discuss the causes of the observed advection process and the effects of the unsteadiness on the second moments. The time evolution of turbulent kinetic energy, for instance, reveals that an advection-like phenomenon is also present at a very low reduced frequency, but its dynamic behaviour is completely different from that of the intermediate frequency ($k=1$). At the intermediate frequency the entire recirculation region is advected downstream, keeping its shape. The advected structure is rotational in nature, and moves at constant speed. In contrast, in the low-frequency case the advected fluid originates at the reattachment point, and the structure is shear-dominated. Particle pathlines reflect the fact that the flow at the low frequency is quasi-steady-state, but show peculiar differences at the intermediate frequency, in which the flow response to the freestream forcing depends on the particle positions in the wall-normal direction.
The present work is devoted to the analysis of drop impact on a deep liquid pool, focusing on the high-energy splashing regimes caused by large raindrops at high velocities. Such cases are characterized by short time scales and complex mechanisms, thus they have received very little attention until now. The BASILISK open-source solver is used to perform three-dimensional direct numerical simulations. The capabilities of octree adaptive mesh refinement techniques enable capturing of the small-scale features of the flow, while the volume of fluid approach combined with a balanced-force surface-tension calculation is applied to advect the volume fraction of the liquids and reconstruct the interfaces. The numerical results compare well with experimental visualizations: both the evolution of crown and cavity, the emanation of ligaments, the formation of bubble canopy and the growth of a downward-moving spiral jet that pierces through the cavity bottom, are correctly reproduced. Reliable quantitative agreements are also obtained regarding the time evolution of rim positions, cavity dimensions and droplet distributions through an observation window. Furthermore, simulation gives access to various aspects of the internal flows, which allows us to better explain the observed physical phenomena. Details of the early-time dynamics of bubble ring entrapment and splashing performance, the formation/collapse of bubble canopy and the spreading of drop liquid are discussed. The statistics of droplet size show the bimodal distribution in time, corroborating distinct primary mechanisms of droplet production at different stages.
It is theoretically known that an isotropic chemically active particle in an unbounded solution undergoes symmetry breaking when the intrinsic Péclet number ${{Pe}}$ exceeds a finite critical value (Michelin et al., Phys. Fluids, vol. 25, 2013, 061701). At that value, a transition takes place from a stationary state to spontaneous motion. In two dimensions, where no stationary state is possible in an unbounded domain, a linear stability analysis in a large bounded domain (Hu et al., Phys. Rev. Lett., vol. 123, 2019, 238004) reveals that the critical ${{Pe}}$ value slowly diminishes as the domain size increases. Motivated by these findings, we here consider an unbounded domain from the outset, addressing the two-dimensional problem of steady self-propulsion with a focus on the limit ${{Pe}}\ll 1$. This singular limit is handled using matched asymptotic expansions, conceptually decomposing the fluid domain into a particle-scale region, where the leading-order solute transport is diffusive, and a remote region, where diffusion and advection are comparable. The expansion parameter is provided by the product of ${{Pe}}$ and $U$, the unknown particle speed (normalised by the standard autophoretic scale). The problem is unconventional in that the scaling of $U$ with ${{Pe}}$ must be determined in the course of the perturbation analysis. The resulting approximation, $U=4\exp ({-2/{Pe}-\gamma _{E}-1})/{{Pe}}$ ($\gamma _{E}$ being the Euler–Mascheroni constant), is in remarkable agreement with the numerical predictions of Hu et al. in the common interval of validity.
We construct reduced-order models of aeroacoustic sources for single and twin subsonic jets ($M_j=0.9$, $Re=3600$), with the goal of accurately recovering the far-field sound over a wide band of frequencies $St=[0.07,1.0]$ and directivity angles $\phi = [30^{\circ },120^{\circ }]$ within a subdecibel level accuracy. These models are realized via combining spatio-temporally coherent spectral proper orthogonal decomposition (SPOD) modes extracted directly from Lighthill's stress tensor, itself calculated using large-eddy simulation (LES). We consider two sets of twin subsonic jets of diameter $D$ each, with spacings of $0.1D$ and $1D$, where the jets merge upstream and downstream of breakdown, respectively. The closely spaced twin jet decays the slowest due to reduced turbulent stresses which are, however, more broadband due to early merging. Such jets show strong shielding in the plane of jets, especially at shallow directivity angles where sound levels may drop below that of the single jet. The farther spaced twin jets have dynamics more akin to the constituent single jet with turbulent fluctuations peaking here at $St=0.34$, but showing very little shielding, with their overall sound pressure level (OASPL) mostly linked to the nature of extra flow structures created during merging. Three-dimensional, energy-ranked, coherent structures for twin jets exhibit rather poor low-rank behaviour, especially at the far-field spectral peak $St=0.14$. At $St \gtrsim 0.3$, the SPOD wavepackets show strong visual coherence, resembling Kelvin–Helmholtz instability modes upstream of breakdown, while at the lower frequencies, there is very little spatial coherence with wavepackets peaking downstream of breakdown. Although the leading SPOD modes radiate poorly, reduced-order models using a subset of them, up to $45$ SPOD modes per frequency, show a remarkable match (within $1$ dB) against the LES-predicted sound over $0.1 \lesssim St \lesssim 0.5$, at all angles investigated. At other frequencies, the closely spaced twin jet shows more error, due to its greater hierarchy of spatio-temporal structures, showing slower convergence at the shallower angles.
We present new numerical solutions for nonlinear standing water waves when the effects of both gravity and surface tension are considered. For small values of the surface tension parameter, solutions are shown to exhibit highly oscillatory capillary waves (parasitic ripples), which are both time- and space-periodic, and which lie on the surface of an underlying gravity-driven standing wave. Our numerical scheme combines a time-dependent conformal mapping together with a shooting method, for which the residual is minimised by Newton iteration. Previous numerical investigations typically clustered gridpoints near the wave crest, and thus lacked the fine detail across the domain required to capture this phenomenon of small-scale parasitic ripples. The amplitude of these ripples is shown to be exponentially small in the zero surface tension limit, and their behaviour is linked to (or explains) the generation of an elaborate bifurcation structure.
In the present study, we investigate the compressibility effects in supersonic and hypersonic turbulent boundary layers under the influence of wall disturbances by exploiting direct numerical simulation databases at Mach numbers up to 6. Such wall disturbances enforce extra Reynolds shear stress on the wall and induce mean streamline curvature in rough wall turbulence that leads to the intensification of turbulent motions in the outer region. The turbulent and fluctuating Mach numbers, the density and the velocity divergence fluctuation intensities suggest that the compressibility effects are enhanced by the increment of the free-stream Mach number and the implementation of the wall disturbances. The differences between the Reynolds and Favre average due to the density fluctuations constitute approximately $9\,\%$ of the mean velocity close to the wall and $30\,\%$ of the Reynolds stress near the edge of the boundary layer, indicating their non-negligibility in turbulent modelling strategies. The comparatively strong compressive events behaving as eddy shocklets are observed at the free-stream Mach number of $6$ only in the cases with wall disturbances. By further splitting the velocity into the solenoidal and dilatational components with the Helmholtz decomposition, we found that the dilatational motions are organized as travelling wave packets in the wall-parallel planes close to the wall and as forward inclined structures in the form of radiated waves in the vertical planes. Despite their increased magnitudes and higher portion in the Reynolds normal and shear stresses, the dilatational motions show no tendency of contributing significantly to the skin friction and the production of turbulent kinetic energy due to their mitigation by the cross-correlation between the solenoidal and dilatational velocity components.
The lift generation mechanism of leading-edge vortex (LEV) in the case of a pitching and plunging plate is studied using an experimental approach and the improved discrete vortex method in this research. A formation condition of the secondary structure is introduced into the traditional discrete vortex method to compensate for the shortcomings in the simulation of the viscous effect between LEV and plate. The simulation of the secondary structure helps the improved method perform better in flow-field reconstruction and lift prediction. Accordingly, the lift generation mechanism of the LEV and influence of the secondary structure are studied. The lift contribution of the vortex structure is isolated and linearly decomposed into two parts according to sources of flow field: the quasi-potential flow part and the vortex-induced flow part. The vortex lift is defined as the lift contribution of the vortex structure in vortex-induced flow, which gives a new insight into the production of lift of the LEV. The lift generation mechanism through the discrete vortex method is verified and extended in viscous flow through experimental measurement. In addition, a vortex lift indicator based on the reverse flow of the LEV is proposed to examine the change of vortex lift in experimental measurement. The flow mechanism for the decline of vortex lift for different maximum effective angles of attack is revealed based on the vortex lift indicator. Furthermore, for the LEV-dominating flow, the indicator can also be applied in estimating the maximum value and corresponding critical time of overall lift in experiments.
This article presents a first-principles model for electrosprays operating in the ion emission regime. The model considers ion emission from a Taylor cone anchored on a tubular emitter, including the fluid dynamics as well as the electrostatic interaction between the liquid, the electrodes and the ion beam. The model accounts for the self-heating of the liquid due to ohmic and viscous dissipation, and the associated variation of the viscosity and electrical conductivity with temperature. The numerical solution reproduces the experimental phenomenology of the ion emission regime (e.g. current levels, the high sensitivity to the ion solvation energy, the proportionality between the emitted current and emitter potential, etc.), and other aspects of the underlying physics such as the coupling between ion emission and self-heating of the liquid. The numerical solution is also used to validate a simpler analytical model that yields scaling laws for the emitted current, and for the characteristic length, current density and electric field of the emission region. The analytical model also provides liquid-dependent criteria for the onset of the ion emission regime.
The description of weakly nonlinear water-wave evolution over a horizontal bottom by the integro-differential Zakharov equation, because of utilising the underlying Hamiltonian structure, has many advantages over direct use of the Euler equations. However, its application to finite-depth situations is not straightforward since, in contrast to the deep-water case, the kernels governing the four-wave interactions are singular, as well as the kernels in the canonical transformation that removes non-resonant interactions from the original equations of motion. At the singularities, these kernels are finite but not unique. The issue of how to use the Zakharov equation for finite depth and whether it is possible at all was debated intensely in the literature for decades but remains outstanding. Here we show that the absence of a limit of the kernels at the singularities is inconsequential, since in the equations of motion it is only the integral that matters. By applying the definition of the Dirac-$\delta$, we show that all the integrals involving a trivial manifold singularity are evaluated uniquely. Therefore, the Zakharov evolution equation and the nonlinear canonical transformation are only apparently singular. The findings are validated by application to examples where predictions based on the Zakharov equation are compared with known solutions obtained from the Euler equations.