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Many species of spittlebugs (Auchenorrhyncha: Cercopidae) use sugarcane and other grasses as host plants, and when damage is extensive they are considered pests leading tom economic losses. Mahanarva fimbriolata and Mahanarva spectabilis are the most common in sugarcane and can be distinguished mainly by genital morphology. Recently, another morphotype of Mahanarva occurring in sugarcane fields that did not match the morphologies of either of these Mahanarva species mentioned above has been widely collected in Brazil, raising doubts on the identification of Mahanarva species using sugarcane. Accurate specimen identification is critical for sugarcane pest management, because misidentifications can lead to economic losses and inefficient control strategies. Thus, we combined morphology, geometric morphometrics, and molecular techniques to investigate the hypothesis that this morphotype could be considered a new species of Mahanarva. Morphological analyses included examination of male genitalia and tegminal colouration patterns. We also quantified hindwing shapes using geometric morphometrics; and performed a phylogenetic analysis using the mitochondrial COI gene. Morphological evidence distinguished the new morphotype through unique traits in male genitalia. Geometric morphometrics reliably separated species, with over 89% classification accuracy. Molecular analyses confirmed the morphotype as a distinct lineage closely related to M. fimbriolata and M. spectabilis. Thus, we describe M. diakantha sp. n., demonstrating the effectiveness of an integrative approach in resolving taxonomic challenges. Additionally, we provide formal diagnoses for M. fimbriolata and M. spectabilis. This work underscores the importance of precise taxonomy in agroecosystems, supporting sustainable pest management practices.
Parasitoids are effective biocontrol agents against Spodoptera frugiperda. Here, we studied four egg parasitoids (Trichogramma chilonis, Trichogramma pretiosum, Trichogrammatoidea bactrae, and Telenomus remus) on S. frugiperda eggs under laboratory conditions [25 ± 1 °C, 70 ± 5% RH, 14/10-h (L:D) photoperiod]. Then, Tr. chilonis (the best trichogrammatid species in the laboratory) was studied inside cages (1 m × 1 m) under maize-field conditions. Egg-to-adult periods (days) were recorded among different species, with Te. remus having the longest (10.00 ± 0.89 days) and Tr. bactrae the shortest (7.80 ± 0.66 days) periods. Furthermore, Te. remus exhibited the greatest adult longevity (days) for both males and females, with and without food. Lifespan parasitism under laboratory conditions was the highest for Te. remus (73.60 ± 7.23 eggs), followed by Tr. chilonis (45.40 ± 2.56 eggs) and Tr. pretiosum (42.00 ± 3.70 eggs). Adult emergence (%) was always higher than 90% and the sex ratio was higher than 0.60 for the studied egg parasitoid species. In the cage experiments set up in uncontrolled field conditions, different release densities of Tr. chilonis (50,000, 100,000, and 150,000 parasitoids/ha) were studied. Release densities of 100,000 and 150,0000 parasitoids per hectare were the most effective rates for managing S. frugiperda, with recorded egg parasitism of 71.36% and 72.88%, respectively. These findings provide crucial insights into the biological attributes and parasitism potential of these parasitoids, indicating the optimal release density of 100,000 Tr. chilonis/ha as an integrated pest management strategy against S. frugiperda in maize ecosystems.
Freshwater parasitic copepods appear to exhibit great taxonomic diversity. However, little is known about gene flow between species or whether there is incongruence between morphological and phylogenetic species definitions. Additionally, little is known about what evolutionary factors may contribute to speciation across various lineages. The copepod genus Salmincola, which includes common ectoparasites of fishes in the family Salmonidae, is distributed throughout the northern hemisphere and is a good model to demonstrate limited taxonomic understanding. Much of the regular scholarly output regarding Salmincola copepods comes from fisheries management agencies, where they are considered a pest species. Within a geographic region, Salmincola copepods of the same species are often found infecting their hosts at substantially different rates across different water bodies. However, present taxonomic definitions of Salmincola are based on decades old morphological descriptions, which were limited in geographic scope and number of specimens examined. There is a strong possibility that traditional species definitions in this genus, based on host species along with morphology, are missing cryptic diversity that may explain differences in infection intensity across environments. This review outlines the current scientific limitations of understanding of this genus and provides suggestions for how adding genetic data could inform taxonomic revisions, as well as clarifying connections between genetic differentiation and infection dynamics across localities.
A survey was conducted among teak and melina growers across Costa Rica to assess their perceptions of problematic weed species and to document current weed management practices in forest plantations. A total of 180 farmers were selected from six provinces, yielding an 87% response rate. Results revealed that 47% of respondents had established plantations on formerly forested land, while 43% did so on previously used pastureland. Most farmers employed an integrated approach toward weed management that included manual, mechanical, and chemical methods. The most problematic species cited most frequently included coyol palm, jaraguagrass, orchid vine, raspa guacal, and arrocillo. Herbicide use was widespread, with glyphosate, metsulfuron-methyl, and paraquat being the most common active ingredients, typically applied singly, in mixtures, or sequentially. The findings reveal a heavy reliance on only three herbicides and highlight the need for targeted control of species in the Poaceae family and climbing vines. This work contributes valuable insights into weed dynamics in tropical forest plantations and emphasizes the need for region-specific and sustainable management strategies to mitigate potential productivity constraints and reduce reliance on herbicides.
This study aims to contribute to enhanced food security in Haiti through proposing targeted local interventions. Employing a spatially explicit tool, the research supports decision-making by relating undernutrition to socio-economic conditions and biophysical factors.
Design:
Georeferenced Demographic and Health Survey (DHS) conducted in 2016–2017 combined with spatial environmental information was used for a multivariate linear regression model to identify factors associated with stunting prevalence. Missing data were imputed through kernel density regression. We converted the structural relationship estimated for the territory of Haiti into a decision support tool by adding fixed effects at communal level. Various policy scenarios were analysed.
Setting:
Haiti, with spatial data across the 134 communes.
Participants:
The analysis included 5623 children under five and their mothers, sourced from DHS data.
Results:
Approximately 22 % of all children were stunted. Implementation of the LimitedIntervention development scenario led to a 2·5 % reduction in stunting, while the ModerateIntervention and FullIntervention scenarios achieved more significant reductions of 6 % and 10 %, respectively. Areas with highest stunting incidence benefit most from interventions.
Conclusions:
This tool supports decisionmakers by assessing the impact of interventions at commune level and selecting areas where interventions exert the most significant effects. The study suggests to apply a strategy that starts in relatively safe communes and then scales to other areas. The flexible approach adopted in this study allows applications in other countries or regions to assess the prevalence of undernutrition among children under five.
Sabellaria miryaensis is capable of growing massive reefs of several meters long in the subtidal area. However, no occurrence of S. miryaensis has been recorded in the Arabian Sea since its original description in 1990. This manuscript presents a new record of S. miryaensis in the Bay of Bengal, confirming its presence there. In addition, details on its ecology, including environmental parameters and the associated benthic biodiversity of S. miryaensis reefs are included. Four 1-m2 quadrat samples were collected from subtidal sabellariid reefs (∼15 m depth) off Gopalpur in March 2024, and associated macrofauna were preserved in 5% formalin for taxonomic analysis. Water parameters (temperature, salinity, and pH) and sediment texture were measured using standard field and laboratory protocols. The reef built by this species supports high macrofaunal and meiofaunal diversity, particularly polychaetes and other invertebrates. Morphological analysis confirms its identity with minor intraspecific variations. The reef also serves as a critical habitat for commercially important fish, underscoring its ecological and economic value. These findings highlight the need for further ecological assessments and conservation of sabellariid reef ecosystems in Indian coastal waters.
The fall armyworm, Spodoptera frugiperda (J.E. Smith), is a major invasive pest threatening maize production in India. Temperature strongly influences its development, reproduction and population dynamics. This study evaluated the biological performance of S. frugiperda on maize across seven constant temperature regimes (14 ± 1°C to 38 ± 1°C) under controlled laboratory conditions, using the age-stage, two-sex life table approach. Developmental time decreased significantly with rising temperatures, while survival and fecundity peaked at 26 ± 1°C and 30 ± 1°C. The highest values of net reproductive rate (R0 = 499.91 females/female), intrinsic rate of increase (rm = 0.25 females/female/day), and finite rate of increase (λ = 1.28 females/day) were recorded at 30 ± 1°C, followed by 26 ± 1°C (R0 = 467.32, rm = 0.24, and λ = 1.26, respectively). In contrast, thermal extremes delayed development and adversely affected both survival and reproduction. No development occurred at 38 ± 1°C. Population projections indicated rapid generational turnover at optimal temperatures, with up to nine generations annually at 26 ± 1°C. The temperature range of 26–30 ± 1°C was found to be optimal for both survival and reproduction of S. frugiperda, aligning with kharif season temperatures in North India, particularly Punjab. These conditions promote multiple generations annually, whereas extreme summer or winter temperatures may limit population development. The findings advocate for temperature-informed, location-specific pest control strategies. Intervening during critical developmental windows, especially at the egg and larval stages, can limit population buildup. Insights into the pest’s thermal adaptability contribute to the advancement of climate-resilient, sustainable pest management frameworks for maize systems in North India and similar agroclimatic regions.
To examine how home food inventories and food procurement practices changed due to the COVID-19 pandemic.
Design:
Cross-sectional baseline data from a randomised controlled trial of a home food environment intervention. Telephone interviews were conducted from October 2020 to December 2022.
Setting:
Four 2–1–1 United Way agencies in Georgia, USA.
Participants:
2–1–1 clients (n 602); 80·6 % identified as Black and 90·9 % as women. Mean age was 42·8 (sd = 11·80). The majority were food insecure (73·4 %) and received Supplemental Nutrition Assistance Program (SNAP) benefits (65·8 %).
Results:
A majority of participants reported smaller inventories of fresh fruits and vegetables (65·1 %) and unhealthy snacks (61·6 %) in the home relative to before COVID-19. The majority (55·8 %) also reported decreased shopping for fruits and vegetables and decreased use of fast food for family meals (56·1 %). Over half (56·2 %) started to use a food pantry, and 44·9 % started ordering groceries online due to COVID-19. A COVID-19 stressors scale was significantly associated with decreased odds of a smaller fresh fruit and vegetable inventory (OR = 0·61, CI 0·51, 0·73) and a smaller unhealthy snack inventory (OR = 0·86, CI 0·74, 0·99). COVID-19 stressors were also associated with changed food procurement practices, including increased online grocery shopping (OR = 1·19, CI 1·03, 1·37), and starting to use a food pantry (OR = 1·31, CI 1·13, 1·51).
Conclusions:
The pandemic had a significant impact on home food inventories and procurement practices. Understanding how major events such as pandemics affect home food environments may help to stave off negative nutritional outcomes from similar events in the future.
Sporobolus natalensis (Steud.) T. Durand & Schinz. and Sporobolus pyramidalis P. Beauv., generally known as giant rat’s tail grasses, are two significant weed species that invade summer fields and pastures in the eastern regions of Australia. This study was conducted to examine seed germination behavior, seedling emergence, and the response of these species to various herbicides. Seed germination and seedling emergence were assessed in response to environmental factors, including alternating temperature regimes (15/5, 20/10, 25/15, 30/20, and 35/25 C), light conditions (dark and light/dark), osmotic potentials (0, −0.1, −0.2, −0.4, −0.8, and −1.6 MPa), and seed burial depths (0, 0.5, 1, 2, and 4 cm). Furthermore, the efficacy of several post-emergence herbicides was evaluated in pots under outdoor environmental conditions. Germination was higher under light/dark (12-h light/12-h dark) conditions than under continuous darkness (24 h). The seeds of both species exhibited significantly higher germination (>95%) under 12-h light at higher temperatures (35/25 C) compared with low (20/10 C) or medium (25/15 C) temperatures. The osmotic potential required to inhibit 50% of maximum germination was −0.77 MPa for S. natalensis and −0.59 MPa for S. pyramidalis. Seedling emergence decreased with increasing burial depth, with no emergence observed from seeds buried at depths of 4 cm. Applying herbicides significantly reduced both species’ seedling survival and dry matter. The most effective herbicides for controlling spring-germinated S. pyramidalis and S. natalensis were haloxyfop, clethodim, butroxydim, glyphosate, glufosinate, and paraquat, which provided satisfactory control of both species. The findings from this study can be used to develop effective management strategies for controlling S. pyramidalis and S. natalensis in agricultural systems.
To cognitively test questions for inclusion in a national nutrition survey, ensuring the questions are interpreted as intended, and to inform further improvements.
Design:
A draft nutrition survey questionnaire was developed based on existing questionnaires and expert input. Twelve questions on dietary habits and food security were selected for cognitive testing as these were newly developed, amended from existing questions or identified to no longer reflect the current food environment or concepts. Cognitive interviews were conducted using both think-aloud and probing techniques to capture respondents’ thought processes used to arrive at an answer. Interviews were audio-recorded and transcribed verbatim. Qualitative data were analysed for recurring patterns and unique discoveries across the survey questions.
Setting:
New Zealand.
Participants:
Sixty-eight participants aged 11 years and older, representing diverse socio-demographics including gender, ethnicity and education level.
Results:
Three main cognitive challenges were identified: (1) interpreting ambiguous terms, (2) understanding dietary or technical terms and (3) following complex or unclear instructions. Questions were refined based on the study findings and further advice from experts in nutrition and survey design to enhance participant understanding and accuracy.
Conclusions:
The cognitive testing findings and expert input led to the refinement and potential improvement of selected questions for inclusion in a national nutrition survey. Changes included simplified terminology, clearer instructions, improved examples and better question order. Our methodological approach and findings may be valuable for those designing similar questions for dietary surveys.
Sir Ernest Shackleton’s ship, Endurance, was crushed by Antarctic sea ice and sank in November 1915. Since then, it has been widely considered that Endurance was the strongest polar ship of its time and was lost because ice tore away the rudder. Based on expedition diaries, Shackleton’s correspondence, and structural analysis, this paper shows that Endurance was not among the strongest ships of its time and that the rudder was not the main cause of the vessel sinking. While the final reason was tearing off the keel, Endurance sank because the vessel was simply crushed in compression by ice. This is not surprising, Endurance was not designed for compressive conditions in the Antarctic pack ice, but for easier conditions at the ice edge in the Arctic. The weakest part of its hull was the engine room area, which was not only larger than in other early Antarctic ships but also lacked beams to give strength against compression by ice. Comparison with other wooden polar ships is not favourable for Endurance: ships designed for compressive pack ice were stronger. It is also evident from archive research that Shackleton was well aware of the weaknesses of Endurance even before his expedition set sail for Antarctica.
Accurate characterization of helminth communities in amphibian hosts is essential for understanding host-parasite dynamics in changing environments. This study presents an integrative parasitological survey of Bufotes viridis populations in eastern Slovakia, using both morphological and molecular methods. A total of 61 road-killed individuals collected across 13 localities were examined for helminth presence. Only nematodes were detected, encompassing 3 families, Rhabdiasidae, Molineidae and Cosmocercidae. Four nematode species were recorded, Rhabdias rubrovenosa, Oswaldocruzia filiformis, O. ukrainae and Aplectana linstowi, and a further unidentified Cosmocerca species. Notably, R. rubrovenosa seems to be a new or previously misidentified helminth species found in Slovakia. Cosmocercidae represented the most abundant family, while Molineidae occurred scarcely. Each species was characterized genetically – for the members of Rhabdiasidae and Cosmocercidae, partial 18S rDNA, complete ITS1, complete 5.8S rRNA, complete ITS2 and partial 28S rDNA sequences were amplified, whereas for representatives of Molineidae, partial COI sequences were obtained. These results underscore the utility of combining molecular and morphological tools in helminth biodiversity studies and provide updated baseline data on nematode infections in B. viridis within an anthropogenically influenced landscape. Despite visual patterns indicating differences in the community compositions of nematode families between urban and rural localities, multivariate analyses testing revealed no significant differences.
Bubble tea is known to have adverse health impacts due to its high sugar content. However, the influence of digital marketing on its consumption, especially among young people, remains unclear. This study aimed to describe the digital marketing strategies of Chinese bubble tea brands.
Design:
A content analysis of all marketing posts made by the top three Chinese bubble tea brands (by market share) – XIXUE, HEYTEA and NAYUKI – on Bilibili between 1 January 2023 and 31 December 2023.
Setting:
Bilibili, a popular social media platform among Chinese young people, in 2023.
Participants:
Not applicable.
Results:
Branding is central to the digital marketing strategies of bubble team brands, with the majority of posts using brand logos (99 %), branded effects (80·1 %) and branded characters (63 %), including children’s characters (19 %). Marketing strategies promoting user interaction were also common, reflected in the frequent use of hashtag campaigns (63 %), general engagement strategies (43 %) and competitions (10 %). Cultural elements that are integrated into the marketing message to resonate with the audience’s cultural identity were present in 47 % of posts.
Conclusions:
Bubble tea brands are using a range of digital marketing strategies to engage consumers and build brand presence in the competitive bubble tea market in China. Measures to protect young consumers from the exposure of such marketing should be considered as a way of improving population diets and reducing excess weight gain.
This descriptive and exploratory observational case series examined intestinal colonisation and subsequent bacteraemia due to ESBL-producing Klebsiella pneumoniae (ESBL-Kp) in preterm neonates in Morocco. Prospective bacteriological cultures and antibiotic susceptibility testing were supported by phenotypic methods, including Brilliance ESBL Agar and the NG-Test CARBA-5 assay, for the rapid detection of ESBL and carbapenemase producers. Molecular analysis using PCR was also undertaken to identify specific resistance genes. A total of 567 rectal swabs were collected from 339 preterm neonates, yielding 293 K. pneumoniae isolates. ESBL-producing strains were identified in 53.6% of the neonates (182/339). Detected resistance genes included blaSHV (26.3%), blaCTX-M-1 (42.8%), blaTEM (30.2%), blaOXA-48 (50.0%), blaNDM(15.3%), and blaVIM (4.9%). Principal risk factors for colonisation were low birth weight (OR 1.69), very preterm birth (OR 6.24), enteral tube feeding (OR 2.02), and prolonged use of third-generation cephalosporins (OR 1.26). Among the neonates studied, 32 (9.4%) developed healthcare-associated bacteraemia, with 56.2% of these cases preceded by intestinal colonisation with ESBL-Kp. Clinically, severe respiratory distress and alveolar haemorrhage were strongly associated with increased mortality (aRR = 29.32 and 4.45, respectively). The findings highlight the clinical importance of early screening to guide infection control and antimicrobial stewardship in neonatal intensive care settings.
This study was conducted to evaluate the supplementation of anionic salt products to pre-partum diets on dry matter (DM) intake, nutrient digestibility, blood metabolites and milk composition of cows during the transition period. Twenty-four Holstein cows [614 ± 21.3 kg body weight (BW) and 2.0 ± 0.9 parity number] were blocked by parity and expected calving date and randomly assigned to the following treatments: Animate (ANI), anionic salts (Animate, Phibro Animal Health, Teaneck, US) fed at 2.85 %DM; or Meganion (MEG), anionic salts (MegAnion, Barentz Animal Nutrition, Hoofddorp, The Netherlands) fed at 1.85 %DM. Treatments targeted -150 mEq/kg diet DM and were provided starting at 30 d of the expected calving date. Treatment effects were evaluated until 56 days in milk. Pre-partum diets presented -55.9 and -32.1 mEq/kg diet DM for ANI and MEG, respectively. Dry matter intake and BW were similar across treatment groups during the pre-partum and post-partum periods. Post-partum body condition score (BCS) tended to be greater for MEG. Pre-partum urine pH was similar between treatment groups (6.33 and 6.49 for ANI and MEG, respectively). Cows fed MEG had greater neutral detergent fibre digestibility on weeks 5 (53.2 vs. 49.8 %) and 6 (53.5 vs. 49.6 %) of lactation than ANI. Serum concentration of Mg was greater in cows fed MEG during the pre-partum. No treatment differences were found for blood concentrations of ionised Ca, non-esterified fatty acids, beta-hydroxybutyrate or insulin. Milk yield was not affected. Both anionic salts maintained adequate calcium metabolism during the transition period and resulted in similar performance.
Russia’s full-scale invasion of Ukraine profoundly disrupted Arctic governance, challenging the long-standing notion of Arctic exceptionalism and creating enduring turbulence. While scholarly debate has largely focused on geopolitical and institutional consequences, the local-level impacts remain underexamined. This study investigates adaptive governance (AG) responses to the war’s effects in Norway’s northernmost counties, Troms and Finnmark, which share a direct border with Russia. The analysis draws on the concepts of crisis, turbulence, and AG, situating them within broader scholarship on how decision-making сenters respond to crises and turbulence and political adaptation. It examines stakeholder responses across four key domains: civilian preparedness, international cooperation, infrastructure development, and the economic repercussions of sanctions. Based on 19 semi-structured interviews, policy documents, and media analysis, the study reveals both adaptation and persistent challenges shaped by pre-existing governance structures, demographic and economic conditions, and past cooperation with Russia. The study contributes to AG literature by unpacking the interplay between strategies, highlighting structural constraints, and demonstrating how geopolitical disruptions shape local governance in strategically significant environments.
Cereal rye (Secale cereale L.) is grown as a cover crop due to its ability to enhance soil health and suppress weeds through allelopathy, but germination responses of different weed species to allelochemicals have not been studied for seeds varying in age and consequently vigor. This study investigated the allelopathic effects of cereal rye on the germination of Palmer amaranth (Amaranthus palmeri S. Watson), large crabgrass [Digitaria sanguinalis (L.) Scop.], giant foxtail (Setaria faberi Herrm.), and lettuce (Lactuca sativa L.). Seeds were germinated in vitro in media with allelochemicals secreted by roots of cereal rye lines varying in allelopathic activity. Seeds were subjected to an accelerated aging treatment to modify their vigor. Results showed that aged seeds exhibited 31% lower germination than non-aged seeds. The magnitude of the germination reduction due to the presence of allelochemicals was species dependent. In the absence of allelochemicals, L. sativa exhibited a 20% reduction in germination due to aging, while the reduction was greater than 60% when allelopathy and aging were combined. Non-aged S. faberi seeds increased germination under low allelopathy conditions, with 20% greater germination compared with the non-treated control. Digitaria sanguinalis did not respond to seed aging, and the high allelopathy treatment reduced germination less than 10%. Amaranthus palmeri exhibited the highest germination and was the only species that was not affected by the seed aging and allelopathy treatments. Germination rate was reduced by seed aging and to a lesser extent by allelochemicals. Results suggest that incorporating allelopathic S. cereale varieties in cover crop rotations can reduce weed populations. However, the age structure of the seedbank might determine the importance of allelochemicals for emergence at the species level, likely causing weed community shifts.