Widespread resistance to selective postemergence herbicides has led to increased use of preemergence herbicides to control rigid ryegrass (Lolium rigidum Gaudin), the major weed of southern Australian cropping systems. Seeds of L. rigidum are dormant at maturity, leading to staggered germination across the growing season and avoidance of pre-sowing knockdown herbicides by the later-germinating cohorts. Although it is well known that this selects for higher seed dormancy in intensively cropped areas, there is less information on whether dormant seeds respond differently to preemergence herbicides applied at sowing. To address this, seeds of field-collected L. rigidum populations were divided into dormant and nondormant (afterripened) subsamples and treated with sublethal rates of three preemergence herbicides in order to monitor seedling emergence and seed persistence over 6 mo. The presence of prosulfocarb and pyroxasulfone eliminated the nearly 4-fold increase in seedling emergence that typically results from afterripening, while trifluralin was partially inhibitory. In all treatments, the proportion of viable seeds remaining in the soil after 6 mo was negligible (≤3% of the viable seeds originally sown) for both the dormant and nondormant seeds. Application of radiolabeled herbicides to soil and seeds showed that the herbicides persisted in the seed tissue for longer than in the bulk soil. Therefore, the presence of dormant L. rigidum seeds in the soil seedbank is unlikely to result in cohorts that can avoid preemergence herbicides.

Italian ryegrass [Lolium perenne L. ssp. multiflorum (Lam.) Husnot] is a significant weed in winter wheat (Triticum aestivum L.), corn (Zea mays L.), soybean [Glycine max (L.) Merr.], and peanut (Arachis hypogaea L.) crops in Alabama. In response to reports of herbicide failure, field surveys were conducted in these cropping systems across Alabama in 2023. The objectives were to document the distribution of herbicide resistance in the collected L. perenne ssp. multiflorum populations. Populations were evaluated in a greenhouse for sensitivity to herbicides representing three modes of action: an acetolactate synthase (ALS) inhibitor (pyroxsulam), two acetyl-coenzyme A carboxylase (ACCase) inhibitors (fluazifop-butyl and clethodim), and a 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) inhibitor (glyphosate). Herbicide screenings were followed by dose–response assays of the most resistant L. perenne ssp. multiflorum population for each herbicide at eight rates (0.5, 1, 2, 4, 8, 16, 32, and 64×) compared with a susceptible population at six rates (0.0625, 0.125, 0.25, 0.5, 1, and 2×). Out of 44 populations evaluated, 21%, 11%, 25%, and 2% were found resistant to glyphosate, fluazifop-butyl, pyroxsulam, and clethodim, respectively. Resistance levels were confirmed to be 192-, 14-, 90-, and 738-fold for glyphosate, fluazifop-butyl, pyroxsulam, and clethodim, respectively. Mutation detection studies revealed specific mutations: Asp-2078-Gly in the ACCase gene, Pro-106-Ser in the EPSPS gene, and a novel Arg-421-Thr mutation in the ALS gene.

This study provides a comprehensive analysis of the morphology and genetics of Raillietiella gigliolii, an endoparasitic pentastomid found in amphisbaenians. The research was based on specimens deposited in the Universidade Regional do Cariri (URCA), as well as newly collected individuals from the Brazilian Caatinga. Detailed morphological descriptions were carried out, including measurements of the hooks, cephalothorax, tail, buccal cadre, and the copulatory spicule in males. In parallel, the first molecular characterisation of this species was performed, targeting the mitochondrial COI gene (barcode region). All specimens exhibited consistent morphotypes, particularly in the shape of the hooks, with no observable variation between males and females, nor between individuals parasitising different hosts (Amphisbaena alba and A. vermicularis). Molecular analyses revealed a well-supported monophyletic clade, with no detectable genetic divergence among individuals, confirming both the morphological stability and genetic delimitation of the species. These findings support the recognition of R. gigliolii as a clearly delineated species, currently restricted to amphisbaenians, which does not exhibit significant morphological variability, in contrast to other congeners.

This study aimed to evaluate different precision hoeing methods on re-compacted ridges. It also aimed to evaluate the impact of ridge re-compaction on soil temperature and moisture retention. Five weeding trials were conducted in corn fields from 2022 to 2024 using two different ridge cultivators, Glühfosator and Damm Profi. The treatments included hoeing (HOE-2), hoeing combined with band herbicide spraying (HOE-1), hoeing combined with living mulch sown in the ridge valleys (HOE-3), and hoeing combined with postemergence harrow (HOE-4). Nontreated control and broadcast herbicide plots were included as controls. Soil moisture and temperature were recorded at 20-min intervals from May to September. Weed species composition, weed biomass, and corn silage yield were measured. Broadleaf weeds were the dominant weed species observed in all corn trials. In most trials, the hoeing treatments were not significantly different from that of applying a broadcast herbicide. Interrow areas treated with side-cut knives and ridge re-builders (HOE-1, HOE-2, and HOE-3) produced a significantly reduced weed biomass (4 to 55 g m−2) and exhibited high (80% to 96% weed control efficacy (WCE) across all trials. Intrarow-treated areas (i.e., tops of ridges) with a band herbicide (HOE-1), no-till sweeps (HOE-2, HOE-3), and postemergence harrow (HOE-4) resulted in 88% to 100%, 30% to 63%, and 17% WCE, respectively. Depending on corn cultivar and ridge cultivator, the HOE-1, HOE-4, and HOE-2 treatments resulted in corn silage yield that was similar to or greater than that of a broadcast herbicide. Yield was increased by 2000 to 9000 kg ha−1 after the HOE-1 treatment, by 2000 to 5000 kg ha−1 after the HOE-4 treatment, and by 3000 to 6000 kg ha−1, after the HOE-2 treatment. When rainfall was limited, re-compacted ridges demonstrated moisture conservation, which resulted in higher day-warming and lower night-cooling of ridge valleys (compared to ridge areas and flat-tilled beds), whereas when rain is heavy, ridges drained moisture and exhibited higher day-warming and lower night-cooling of ridge areas. These results suggest that precision hoeing on ridges could alternate broadcast herbicide use, while re-compacted ridges prove resilient to extreme rainfall events.

Hospital food services and the resulting food waste impact patient satisfaction, health outcomes, healthcare costs, and the environment. This cross-sectional study assessed food waste and patient satisfaction in five public hospitals in Cyprus, involving 844 inpatients. Patient characteristics and responses to the 21-item Acute Care Hospital Foodservice Patient Satisfaction Questionnaire (ACHFPSQ) were recorded. Plate waste was evaluated using photographs and a five-point visual scale (0 to 1) to estimate food consumption. Hunger and overall satisfaction were also assessed. While 77.8% rated food services as good or very good, food quality received the most negative feedback. Only 31.2% finished their main dish entirely; 29.5% and 26.3% left ¼ and ½, respectively. For dessert, 48.2% finished it, while 13.3% left it untouched. These findings reveal a gap between general satisfaction and perceived food quality, underscoring the need for targeted public health strategies to enhance food quality and reduce waste in hospitals.

Despite the appeal of screening travellers to prevent case importation during infectious disease outbreaks, evidence shows that symptom screening is largely ineffective in delaying the geographical spread of infection. Molecular tests offer high sensitivity and specificity and can detect infections earlier than symptom screening, suggesting potential for improved outcomes. However, they were used to screen travellers for COVID-19 with mixed success. To investigate molecular screening’s role in controlling COVID-19, and to quantify the effectiveness of screening for future pathogens of concern, we developed a probabilistic model that incorporates within-host viral kinetics. We then evaluated the potential effectiveness of screening travellers for influenza A, SARS-CoV-1, SARS-CoV-2, and Ebola virus. Even under highly optimistic assumptions, we found that the inability to detect recent infections always limits the effectiveness of traveller screening. We quantify this fundamental limit by proposing an estimator for the fraction of transmission that is preventable by screening. We also demonstrate that estimates of ascertainment overestimate reductions in transmission. These results highlight the essential role that quarantine and repeated testing play in infectious disease containment. Furthermore, our findings indicate that improving screening effectiveness requires the ability to detect infection much earlier than current state-of-the-art molecular tests.

Bacteria play a fundamental but often overlooked role in shaping insect communities in cattle (Bovidae) dung. To direct attention to this role, three experiments were performed with cattle dung autoclaved to reduce bacterial activity and the associated release of volatile organic compounds (VOCs) that attract coprophilous insects to deposits. In the first experiment, and consistent with expectations, fewer insects were recovered in pitfall traps baited with autoclaved versus control dung. In the second experiment, there was generally lower recovery of insects developing in autoclaved versus control pats colonised in the field. This result was attributed to reduced oviposition and lower survival of immature insects in the autoclaved pats. In the third experiment, no effect of autoclaved versus control dung was detected on the reproductive success of the dung beetle Onthophagus taurus (Linnaeus) (Coleoptera: Scarabaeidae), possibly because adults carry with them the requisite bacteria for larval development. In summary, faecal bacteria produce VOCs to directly affect the composition of the insect species that colonise and oviposit in cattle dung. The survival of their progeny is affected by faecal bacteria that provide a source of nutrients or may be pathogenic.

Anonymous online surveys using financial incentives are an essential tool for understanding sexual networks and risk factors including attitudes, sexual behaviors, and practices. However, these surveys are vulnerable to bots attempting to exploit the incentive. We deployed an in-person, limited audience survey via QR code at select locations in North Carolina to assess geolocation application use among men who have sex with men to characterize the role of app usage on infection risk and behavior. The survey was unexpectedly posted on a social media platform and went viral. Descriptive statistics were performed on repeat responses, free-text length, and demographic consistency. Between August 2022 and March 2023, we received 4,709 responses. Only 13 responses were recorded over a 6-month period until a sharp spike occurred: over 500 responses were recorded in a single hour and over 2,000 in a single day. Although free-text responses were often remarkably sophisticated, many multiple-choice responses were internally inconsistent. To protect data quality, all online surveys must incorporate defensive techniques such as response time validation, logic checks, and IP screening. With the rise of large language models, bot attacks with sophisticated responses to open-ended questions pose a growing threat to the integrity of research studies.

The polar regions are famous for being inhospitable, difficult to access, and one of the final frontiers for exploration. The late 19th and early 20th centuries were filled with explorers seeking the achievement of being the first person to the Pole. These harrowing stories have action and adventure but lack a critical component: women. Women historically have not played a primary role in polar research or exploration. Many barriers to access existed such as prejudice, lack of education opportunity, and physical restrictions. Today, women have better access to the Antarctic and Arctic for research and research support but still face barriers to equitable participation. A “boys club” environment in stations can lead to women being excluded or subjected to sexual harassment. Despite this, the addition of women is shown to improve team dynamics, morale, and the culture within research stations. Women’s representation in polar research is better today than it’s ever been, yet there is still improvement being made for the future.

The rapid evolution of SARS-CoV-2 has led to the emergence of variants of concern (VOCs) characterized by increased transmissibility, pathogenicity, and resistance to neutralizing antibodies. Identifying these variants is essential for guiding public health efforts to control COVID-19. Although whole genome sequencing (WGS) is the gold standard for variant identification, its implementation is often limited in developing countries due to resource constraints. In Bolivia, genomic surveillance is a challenge due to its limited technological infrastructure and resources. An RT-qPCR-based strategy was designed to address these limitations and detect the mutations associated with VOCs and variants of interest (VOIs). The multiplex RT-qPCR commercial kits AllplexTM Master and Variants I (Seegene®) and the ValuPanelTM (Biosearch®) were used to target mutations such as HV69/70del, E484K, N501Y, P681H, and K417N/T. They are characteristic of the Alpha (B.1.1.7), Beta (B.1.531), Gamma (P.1), Omicron (B.1.1.529), Mu (B.1.621), and Zeta (P.2) variants. A total of 157 samples collected in Cochabamba from January to November 2021 were evaluated, identifying 44 Gamma, 2 Zeta, 20 Mu, and 10 Omicron were identified. The strategy’s effectiveness was validated against WGS data generated with Oxford NanoporeTM technology, showing a concordance rate of 0.96. This highlights the value of the RT-qPCR strategy in guiding the selection of samples for WGS, enabling broader detection of new variants that cannot be identified by RT-qPCR alone.

Predation is a strong driver of prey behaviour and sympatric species are likely exposed to similar selective predatory pressures. We test the hypothesis that this leads to similar anti-predator behaviours using the widespread Neotropical snake tribe Pseudoboini as our model. We reviewed and compiled documented defensive behaviours for all species, adding new unreported behaviours for three species. We used a cluster analysis to generate a matrix of defensive behaviour dissimilarity between species. We then used a phylogenetic generalized linear mixed model to test how behaviour dissimilarity changed with geographical overlap, similarity in ecological traits, and phylogenetic relatedness. Only 41 species had available data on defensive behaviour, with only 22 of those represented in the phylogeny. We found that similarity in defensive behaviour is significantly (albeit not strongly) correlated with geographical overlap, but only for species with similar body sizes. Phylogenetic relatedness by itself was a poor predictor of behaviour dissimilarity. This corroborates our hypothesis that defensive behaviours are spatially structured at larger scales but that this can be modulated by morphological differences. Testing inter-species ecogeographical differences of defensive behaviour and its implications can be broadly applied to other taxa.

Microplastic release in Antarctica is an issue of increasing concern, despite the limited human presence in the region. This study estimates the annual release of microplastics from the wastewaters of scientific facilities through the use of personal care products and laundering. Furthermore, it analyses the most cost-efficient policy interventions to target this pollution. The study has estimated a potential release of 238 kg per year, which is negligible on a continental scale but could have substantial local environmental impacts. A comprehensive cost-efficiency analysis demonstrates that microplastic release can be effectively mitigated through low-cost preventative measures, such as installing washing machine filters and banning hygiene products containing microbeads. Furthermore, the implementation of wastewater treatment systems is suggested as a crucial and long-term cost-effective solution for treating wastewater effluent and removing other pollutants from the Antarctic region. These results provide a framework to inform policy decisions on microplastic release in Antarctica and lay the foundation for improved environmental protection strategies in this sensitive region.

Marbled Teal Marmaronetta angustirostris is a globally threatened species that has been undergoing population declines across much of its range in recent years. This is particularly true in the Middle East and the Caucasus, where the species once commonly bred across much of the region. However, there is a dearth of recent literature and population-level assessments of the species in the countries in this region. For example, the last update of conservation status for Marbled Teal in Armenia was undertaken in 2009 and in Türkiye in 2008. Therefore, this study addresses the urgent need for an updated evaluation of the species’ status in both Armenia and Türkiye. For Armenia, the current population estimate is 8–11 breeding pairs, with a steep decline of 87% between 2003 and 2019. In Türkiye, the species appears to be functionally extirpated from the country due to an absence of breeding in almost a decade and multiple years without any records, with only a handful of wandering individuals detected in recent years. This study highlights the threats facing the species, particularly changes to wetland habitat and quality as well as hunting pressures and illegal poaching. Based on our findings, we propose that the conservation status of Marbled Teal in both countries be updated from “Endangered” to “Critically Endangered”. Finally, we note the conservation requirements for the species in the region and provide a set of recommendations for its protection, including a species recovery plan. Without urgent conservation measures such as the creation of new protected areas and establishment of new populations from released birds, the long-term viability of Marbled Teal populations in the region is in jeopardy.

Limited studies on the seasonality of pharyngitis and tonsillitis suggest subtle but unexplained fluctuations in case numbers that deviate from patterns seen in other respiratory diagnoses. Data on weekly acute respiratory infection diagnoses from 2010–2022, provided by the Polish National Healthcare Fund, included a total of 360 million visits. Daily mean temperature and relative humidity were sourced from the Copernicus Climate Data Store. Seasonal pattern was estimated using the STL model, while the impact of temperature was calculated with SARIMAX. A recurring early-summer wave of an unspecified pathogen causing pharyngitis and tonsillitis was identified. The strongest pattern was observed in children under 10, though other age groups also showed somewhat elevated case numbers. The reproductive number of the pathogen is modulated by warmer temperatures; however, summer holidays and pandemic restrictions interrupt its spread. The infection wave is relatively flat, suggesting either genuinely slow spread or multiple waves of related pathogens. Symptomatic data unambiguously demonstrate existence of pathogens of quite distinct characteristics. Given its consistent year-to-year pattern, identifying these potential pathogens could enhance respective treatment, including antibiotic therapy.

Molecular evidence (28S DNA) has suggested that Triplotaenia undosa from macropodid marsupials is a species complex. Additional data (cox 1) presented in this study confirmed the hypothesis and a morphological examination of all available specimens identified a new species, T. macropodis sp. nov., in the grey kangaroos Macropus fuliginosus and M. giganteus as well as the tammar wallaby, Notamacropus eugenii, and the red kangaroo, Osphranter rufus. The new species differs in the ratio of the number of testes to the number of female genital complexes. Specimens of T. undosa from the swamp wallaby, Wallabia bicolor, the type host, and the common wallaroo, Osphranter robustus, are each genetically distinct, but the fixed material from O. robustus is too fragmentary to permit a detailed morphological description. An amended description and new illustrations of T. undosa from W. bicolor are provided.

Spiders (Araneae) are an abundant and diverse arthropod group that serve important ecosystem functions in boreal forests. Several hundred species across boreal Canada are prey for vertebrates and invertebrates. Spiders are also generalist predators that likely contribute to pest control. Our understanding of spider assemblages, particularly of the arboreal community, is minimal at the stand level in many habitats across Canada. Habitat-specific factors like connectivity, microclimate, and neighbour effects can substantially influence the structure of ecological communities. Well-replicated landscape-scale experimental designs enable us to better understand the structure of arboreal spider communities. Here, we employed beat-sheeting to characterise spider assemblages on balsam fir trees (Pinaceae) from the three most common stand types found in the boreal: coniferous, deciduous, and mixedwood. Fir trees in deciduous stands had greater spider abundance than did the trees in coniferous or mixedwood stands. Neither species diversity nor composition differed significantly among the three stand types. Our results suggest that spiders likely do not recognise “the forest for the trees.”

A bottlenose dolphin, Tursiops sp., stranded on the coast of South Carolina, USA was found to be heavily infected in its intestine by tapeworms, which we identified molecularly. Sequencing of portions of the mitochondrial cytochrome c oxidase I (COI) and nuclear large subunit ribosomal RNA (28S rRNA) genes showed the cestodes to be Diphyllobothrium stemmacephalum, commonly known as a broad tapeworm. Infections of marine mammals by Diphyllobothrium have been previously reported in the Northwestern Atlantic Ocean, but only to genus level. Infection by tapeworms may be rare in dolphins in South Carolina, but because this species is zoonotic, its presence indicates the potential for an emerging public health concern.

When traditional measures for material conditions are scarce or unreliable, body mass, height, and weight are complements to standard income and wealth measures. A persistent question in welfare studies is the 19th century’s 2nd and 3rd quarter’s stature diminution, a pattern known as the antebellum paradox. However, the question may not be well stated nor experienced equally by women and non-white male samples. The late 19th century’s political Granger, Greenback, and Populist movements may have affected farmer and non-farmer’s net nutrition. Despite 19th and early 20th century US political movements, farmers had greater BMIs, taller statures, and heavier weights than non-farmers. From the 1870s through 1890s, women’s body mass, height, and weight increased relative to men. Individuals of African or mixed European-African descent had heavier weights and greater BMIs than their taller, European-white counterparts, indicating that the traditional antebellum paradox needs to include women and non-European males and weight measures.