Two new species of giant lacewings (Neuroptera: Polystoechotidae) are described from Ypresian Okanagan Highlands localities in western North America: Palaeopsychops goodwini sp. nov. and Palaeopsychops barthae sp. nov., both from Republic, Washington, United States of America, and a less well-preserved specimen is treated as Palaeopsychops sp. indet., the first occurrence of the family at McAbee, British Columbia, Canada. These share distinctive wing colouration – dark with a single pale, broad fascia mid-wing.

Animal welfare awareness (AWA) during transportation and in markets is a critical concern in livestock production, influencing the health of animals and other outcomes for stakeholders. Nevertheless, it remains understudied in many developing regions. This study investigates the level of awareness and practices regarding animal welfare during and after transportation among primary stakeholders — sellers, drivers, and buyers — in three livestock markets in Nigeria: Achida, Ikorodu Sabo, and Amansea. A structured survey focusing on the stakeholders’ familiarity with the concept of animal welfare, the Five Freedoms, the Animal Diseases (Control) Act, and encounters with veterinary control posts was conducted across the selected markets between February and July 2024. Furthermore, stakeholders were also questioned about barriers to improving practices. Descriptive and inferential statistical analyses were performed to explore associations between the dichotomised awareness of animal welfare and key variables. A significant association between AWA and market location was revealed, but not with occupation. Further analysis showed that dichotomised awareness of Veterinary Control Posts (VCPs) and the Animal Diseases (Control) Act had significant negative associations with AWA, suggesting complex relationships between legal knowledge and familiarity with the concept of animal welfare. Additionally, transport-related mortality was reported by 70.7% of respondents, with overcrowding and sickness identified as primary causes. However, significant barriers, including economic constraints and a lack of authority to mandate standards, were the leading challenges often faced by stakeholders. The findings underscore the need for targeted interventions and policy reforms, including increased enforcement.

The limited number of herbicides for use in peanut makes it difficult to diversify modes of action to combat advantageous weed species with reports of increasing herbicidal resistance. It is critically necessary to explore both new and repurposed chemistries with different modes of action for potential use on peanut crops. Little research has investigated peanut response to scenarios in which preemergence applications of fluridone or trifludimoxazin are delayed. Replicated field trials using small plots were conducted at the University of Georgia from 2022 to 2024 to identify any deleterious effects of fluridone applied at 126 g ai ha−1 or trifludimoxazin applied at 37 g ai ha−1 1, 3, 5, or 7 d after planting (DAP). The peanut population was not affected, regardless of herbicide or application timing. At 13 DAT, plant growth was reduced by 5% to 9% when fluridone had been applied 1, 3, 5, and 7 DAP. Visual crop growth was reduced by 10% to 19% with applications of trifludimoxazin, with the greatest effect occurring when it was applied at 7 DAP. Trifludimoxazin also caused 7% foliar leaf necrosis when averaged over application timings. Regardless of application timing, peanut height was reduced by both herbicides at 30 DAP but not at 80 DAP. However, at 80 DAP, plant width was reduced by 4% after fluridone and trifludimoxazin had been applied. Peanut yield was not affected by herbicide treatment regardless of when it was applied. Fluridone and trifludimoxazin applied as late as 7 DAP can injure peanut but not affect yield.

Females may adjust how many eggs they lay over the course of their lifetime (i.e., their egg-laying pattern) to bias their investment into either current or future reproduction. Using mate availability cues to bias reproductive investment could ensure that females obtain the benefits of multiple mating when future mate availability is high or low. We studied whether perceived mate availability influenced egg-laying patterns in Teleogryllus oceanicus Le Guillou (Orthoptera: Gryllidae), the Pacific field cricket, and whether variation in those patterns affected females’ future egg-laying or total reproductive output. On hearing the male calling song to simulate high mate availability, females did not alter their egg-laying patterns relative to females that did not hear the song. The lack of influence of perceived mate availability on egg-laying patterns is noteworthy because this treatment affects many other aspects of this species’ reproductive investment. Neither investing highly in current versus future egg-laying nor having a highly variable egg-laying pattern appeared to be costly in this species. Despite consistent conditions and sufficient resources for females during the experiment, our fine-scale study of egg-laying patterns highlights the variability that exists in these patterns, and we speculate on some factors that may drive this variation.

Scrub typhus is a mite-borne infection, largely affecting rural populations in many parts of Asia. This cohort study explored socio-demographic, behavioural, and spatial risk factors at different levels of endemicity. 2206 rural residents from 37 villages in Tamil Nadu, South India, underwent a questionnaire survey and blood sampling at baseline and annually over 2 years to detect sero-conversion. Satellite images were used for visual land use classification. Local sero-prevalence was estimated using 5602 baseline blood samples.

Two hundred and seventy cases of seroconversions occurred during 3629 person-years (incidence rate 78/1000, 95%CI 67, 91). Older age was associated with scrub typhus in crude but not in multivariable analysis adjusting for socio-economic factors. By contrast, the increased risk in females compared to males (RR 1.4) was unaffected by adjusting for confounders. In multivariable analysis, agricultural and related outdoor activities were only weakly associated with scrub typhus. However, agricultural activities were strongly associated with scrub typhus if local sero-prevalence was low, but not if it was high. Females were at a higher risk than males in high-prevalence areas but not in low-prevalence areas. To conclude, agricultural activities were not strongly associated with scrub typhus. Transmission within human settlements may predominate in highly endemic settings.

High-fat diets are closely implicated in the pathogenesis of chronic conditions, including obesity and hepatic steatosis. Recently, coconut oil, which is rich in medium-chain fatty acids, has attracted significant attention for its potential anti-obesity and anti-inflammatory properties. This study aimed to evaluate the effects of medium-chain fatty acids derived from coconut oil on metabolic disorders, particularly fatty liver, using a mouse model established by a high-fat diet. C57BL/6J mice were assigned to either the lard diet group or the coconut oil diet group and fed for 12 weeks. Glucose tolerance was assessed, and biochemical parameters, liver histology, and gene expression in the liver were analysed. Additionally, the concentrations of medium-chain fatty acids within the liver were determined through gas chromatography-mass spectrometry analysis. Mice fed a coconut oil diet exhibited suppressed weight gain and improved glucose tolerance compared to mice fed a lard diet. Furthermore, the coconut oil diet resulted in reduced hepatic fat accumulation, decreased expression levels of genes implicated in inflammation and lipid metabolism within the liver, and higher concentrations of medium-chain fatty acids in the liver. Coconut oil may contribute to the suppression of hepatic fat accumulation in the liver and the prevention of non-alcoholic fatty liver disease/metabolic dysfunction-associated steatotic liver disease by increasing the levels of medium-chain fatty acids in the liver and suppressing the expression of genes implicated in inflammation and lipid metabolism.

Understanding the circadian rhythms of bark and ambrosia beetles (Scolytinae) is crucial for assessing their dispersal strategies, trophic specialisation, and microhabitat preferences. This study investigated circadian rhythms in Scolytinae communities using flight interception traps in an oak forest in the southern part of Czechia. Ordination biplot revealed a flight activity gradient, with nocturnal dispersers distinct from diurnal species. Species richness gradually decreased from the 20:00–24:00 interval through to the 12:00–16:00 interval, with the most notable decline observed between the 08:00–12:00 and 12:00–16:00 intervals. A combination of fourth-corner and partial canonical correspondence analyses identified tribal affiliation, trophic specialisation, and microhabitat preference as key drivers of flight structuring. Members of the tribe Xyleborini showed negative association to the 16:00–20:00 interval. Xylomycetophagous species, such as Xyleborinus saxesenii exhibited multimodal activity peaks, with increased flight from nighttime to early morning. In contrast, species that feed in the phloem such as Scolytus intricatus showed no significant association with any specific time interval. Several species that utilise stumps as potential breeding substrates showed significantly reduced flight activity during the crepuscular period, which supports the hypothesis that microhabitat preference contributes to diel periodicity. Our findings show the complex interplay between abiotic and biotic factors in shaping circadian flight periodicity, which leads to distinct flight activity patterns between Scolytini bark and Xyleborini ambrosia beetles. These results emphasise the ecological significance of maintaining heterogeneous forest structures that provide a balance of shaded and sun-exposed deadwood habitats to support diverse assemblages.

Eclipta [Eclipta prostrata (L.) L.] is an important tropical weed that has recently emerged as a problematic weed in dry direct-seeded rice (Oryza sativa L.) (DSR) fields in China. Understanding its seed germination biology and ecology is crucial for developing integrated weed management strategies in the DSR system. Laboratory experiments were conducted to investigate seed germination of E. prostrata seeds under varying environmental conditions. Germination was greatest under alternating temperature regimes of 25/15 to 40/30 C, whereas it wa-s significantly reduced at 20/10 C and completely inhibited at 15/5 C. Germination was also fully suppressed under continuous darkness, indicating strong light dependency. Eclipta prostrata seeds tolerated a broad range of pH values (4 to 10) with germination rates consistently greater than 95%. However, germination declined sharply under osmotic potentials, falling below 2% at −0.6 MPa, and being completely inhibited at −0.7 MPa. Seeds also showed moderate salt tolerance, with 50% inhibition at 150 mM NaCl and no germination at 300 mM NaCl. Exposure to radiant heat (>90 C for 5 min) prevented germination, suggesting residue burning may be an effective control measure. Seedling emergence was highest (100%) on the soil surface but declined steeply with increasing burial depth, with no emergence observed beyond 0.5 cm. Similarly, surface application of wheat (Triticum aestivum L.) straw residue (2 to 6 Mg ha−1) significantly reduced seedling emergence and biomass. These findings provide essential insights into E. prostrata germination ecology and offer practical implications for its integrated management in DSR systems.

The n-3 index has been proposed as a risk factor for CVD endpoints. However, the association of the O3I defined with different cut-offs and cardiometabolic risk factors has been less studied. This study aimed to investigate the association between two cut-off points of the O3I and cardiometabolic risk factors in Brazilian and Puerto Rican adults. This cross-sectional analysis included 249 Brazilians and 1261 Puerto Ricans, aged 45–75 years. Fatty acids composition was quantified in erythrocyte membranes using GC with a flame ionisation detector. The O3I was categorised as ≤ 4 % (low), > 4–8 % (intermediate) and ≥ 8 % (desirable), and as ≤ 4 % (very low), > 4–6 % (low), > 6–8 % (moderate) and > 8 % (high) in the second cut-off classification. Serum lipids, waist circumference and insulin resistance were measured from standardised protocols. Multivariable-adjusted linear models tested the association between the O3I and cardiometabolic factors. Brazilians had a mean (sd) O3I of 4·65 % (1·19 %) v. 4·43 % (1·14 %) in Puerto Ricans (P = 0·033), with only 1·6 % of Brazilians and 1·2 % of Puerto Ricans presenting a desirable/high O3I. The O3I, as continuous or for > 4 % (v. ≤ 4 %), was inversely associated with TAG, VLDL and TAG/HDL-cholesterol ratio in Puerto Ricans. In Brazilians, an O3I > 6 % (v. ≤ 6 %) was associated with higher total cholesterol, LDL-cholesterol and non-HDL-cholesterol. Both populations presented O3I below the desirable levels, and the magnitude and direction of associations with cardiometabolic factors varied by study and cut-offs, reinforcing the importance of expanding these investigations to more diverse populations.

Winter barley is mainly grown in Europe. Before new varieties are recommended for cultivation, they undergo evaluation in breeding and variety trials. Based on the results of these trials, the stability and adaptability of promising new lines or varieties are assessed. In the present study, based on results from post-registration trials, we compared varieties grown in the 2020/21, 2021/22 and 2022/23 seasons. We fitted two Bayesian mixed models and assessed the stability of the varieties using the posterior estimates of variance components from the preferred model. We also used Bayesian probabilistic methods to recommend the best varieties. Using the probabilistic methods, we identified the varieties that were the most stable and had the highest yield in the barley post-registration trials. The varieties Melia, Mirabelle and Zenek were shown to be the three most stable and highest yielding. Furthermore, these three varieties had the highest joint probability of superior performance and stability. This study demonstrates that probabilistic methods within a Bayesian framework are a powerful tool for recommending the best winter barley varieties. The R-codes for both models are provided in a Supplement.

The Critically Endangered Hooded Grebe Podiceps gallardoi has suffered a population decline of 80% since the 1980s. The evolutionary history and its critical conservation status place it 20th in the EDGE of Existence Bird List (EDGE-ZSL) among the more than 10,000 bird species of the world. The identification of demographically independent units (“management units”) is essential to address appropriate conservation and management strategies for threatened species. Genetic markers can be used to infer isolated populations without the need for logistically expensive banding and recapture. We used blood samples of 71 Hooded Grebes (c.10% of the global population) from three reproductive populations located at different plateaus that hold over 90% of the species’ global population. We analysed genetic population structure using a 353-bp fragment of mtDNA control region and 1,886 RAD loci to study whether Hooded Grebes are philopatric or not. We did not find differences in genetic structure of populations between plateaus indicating that Hooded Grebes do not consistently return to their plateau of origin. Our results are critical to understanding the connection of populations throughout the full annual movement cycle and propose management actions accordingly.

Endothelial progenitor cells (EPCs) are key regulators of vascular homeostasis in both health and disease, playing a crucial role in regenerating the human vascular lining throughout life. These circulating cells can differentiate into mature endothelial cells and are increasingly recognized as important biological markers of vascular function and cumulative risk for various diseases, including cardiovascular conditions. In recent decades, the role of EPCs, particularly the endothelial colony-forming cells (ECFCs) subtype, in pregnancy-related disorders and maternal and neonatal endothelial health has garnered significant attention. Evidence suggests that ECFCs may serve as predictor of future endothelial health in women and their offspring following pregnancy complications, making them particular relevant for research and therapeutic applications in adulthood, as well as potential indicators of vascular health. This review summarizes the evidence on EPCs, specifically ECFCs, as biomarkers of endothelial health in pregnancy, pregnancy-related diseases and ageing, with a focus on maternal and foetal endothelial abnormalities that may serve as prognostic factors for the development of future diseases.

Forest structure has a significant impact on the quality of habitat for various bird communities. In particular, birds that rely on forests, such as woodpeckers, are sensitive to changes in the characteristics of the forest. However, there is limited knowledge on how woodpeckers respond to these changes in forests outside protected areas, and in the highly seasonal Eastern Himalaya. To address this gap, a study was conducted in the differently managed non-protected forests of Darjeeling, Eastern Himalaya, India, spanning an elevation range of 250–2,400 m. The study aimed to identify the key forest characteristics that influence woodpecker diversity at the community and individual species levels. Data on woodpeckers were collected using point counts along transects during the pre-monsoon, monsoon, post-monsoon, and winter seasons. Habitat characteristics were assessed using 20 × 20 m quadrats at each observation point. The study recorded 1,721 individual woodpeckers belonging to 13 species from 3,456 point counts. The results indicated that the basal area and density of snags were the main factors influencing woodpecker diversity. Woodpeckers in the study area showed a significant negative relationship with the basal area, tree density, and tree diameter diversity. This suggests that woodpeckers prefer high snag density but scattered, smaller, and more uniformly sized trees in the study area. Among individual species, the Greater Yellownape Chrysophlegma flavinucha and Grey-capped Pygmy Yungipicus canicapillus Woodpeckers showed a strong preference for high snag density, while Bay Woodpeckers Blythipicus pyrrhotis were closely associated with high canopy cover and denser forests. Seasonal effects had minimal influence on woodpecker diversity in the study area. The study contradicts the typical preference of large woodpeckers for large trees and greater basal areas, despite four large-sized species making up 75% of the woodpecker community in the region. Thus, the findings highlight the importance of considering species-specific, region-specific, and management-specific habitat requirements when developing conservation strategies.