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We assessed the feeding behavior, intake, apparent total tract digestibility, blood parameters, milk yield, milk composition and milk fatty acid profile in dairy goats fed diets containing increasing levels of whole cottonseed (WCS), specifically a control diet with no WCS and diets containing 120, 150 and 180 g WCS/kg DM. Eight lactating dairy goats were distributed in a replicated 4 × 4 Latin square design with four 21-day periods. Rumination and total chewing time increased linearly with WCS inclusion levels. However, no effects on dry matter intake or digestibility were observed. Milk production was similar among treatments, but milk fat and lactose concentration increased linearly with WCS dietary levels, as did monounsaturated and polyunsaturated FA and conjugated linoleic acid concentration in milk fat. This study demonstrates that increasing the inclusion of WCS in the diets of dairy goats offers potential nutritional benefits without negatively impacting intake, digestibility or ruminal fermentation. Notably, WCS-enhanced diets led to improved milk fat composition, with increased concentrations of beneficial FA. These results support the use of WCS as an effective feed ingredient to enhance milk quality in dairy goats, leveraging both the high-energy content and fiber structure of WCS, which promotes rumen health and function without reducing DMI. Dairy goat producers can incorporate up to 180 g WCS/kg DM in diets to produce milk with a healthier fat profile, potentially enhancing its marketability.
What can we learn from Augustine’s preaching about the nature and purpose of preaching? In this paper, I will argue that in his preaching Augustine presents to his audience a theology of words and the Word that achieves what it declares; that is to say, rather than a mere doctrinal curiosity, Augustine’s preached theology of words and the Word accomplishes a homiletical goal that transcends the transmission of an idea and, instead, guides the faithful listener’s heart towards the eternal Word of God through the temporal words of the preacher and the written words of scripture. To put it another way, Augustine’s theology of words and the Word is both a theological claim and a practical pastoral tool.
The aim of this Research Communication was to develop new flow cytometric tools for the fine identification and characterization of milk somatic cells in water buffalo (Bubalus bubalis). Four multicolour panels of antibodies were designed to identify different subsets of live leukocytes and epithelial cells in bulk milk samples. Panel 1, including the CD18/CD172a/CD14/CD16 markers and Live/Dead vitality dye, allowed us to identify total lymphocytes, polymorphonuclear neutrophils (PMN) and monocyte/macrophage subsets. Panel 2 (CD18/CD4/CD8/δ chain/CD335 and Live/Dead dye) allowed us to identify T helper (CD4+), T cytotoxic (CD8+), γδ lymphocytes and NK cells. Panel 3 (CD18/CD79a/CD21 and Live/Dead dye) allowed us to identify total and CD21+ B lymphocytes. Finally, with Panel 4 (CD18/MHC-I/pan Cytokeratin and Live/Dead dye) the epithelial cells were distinguished from leukocytes. In conclusion, we propose a fine characterization of live milk somatic cell (live differential cell count (LDCC)) in buffalo species. In the future the determination of LDCC could used to identify new markers for detecting early inflammatory states of the mammary gland or for monitoring the technological properties of milks of different somatic cell composition.
Buffalo are shy breeders and poor fertility traits are a major hindrance in exploiting the production potential of the animal. This study hypothesizes that polymorphisms in the luteinizing hormone beta (LHβ) gene can affect oestrus behaviour in buffaloes. A total of 100 animals were screened by calculating the heat index (threshold-50) and animals were categorized into two groups (Group1 > 50, Group2 < 50). Animals were subjected to blood sampling, genomic DNA isolation, specific primer based polymerization and sequencing of amplicons. A total of six genomic variations were identified in the gene. c.V15M was a non synonymous mutation found in line with the Hardy Weinberg Equilibrium and was significantly associated with the trait. Functional impact of the variation was determined by three-dimensional structure of the protein. Effect of c.V15M on the functionality of the gene was evident and hypothesis was supported so this can potentially be used as a marker for the future development of superior animal breed or regulating the expression of the gene to get the optimal oestrus cyclicity in river buffalo of Pakistan.
In this Research Communication we describe the application of single-step Bayesian regression (ssBR) models to predict milk yield of Murrah buffaloes. Milk production records of 2,026 cows in their first lactation were used. Using 270-day cumulative milk yield records as phenotype, genomic breeding values were predicted and their accuracies and dispersions were calculated by five methods: BayesA (ssBA), BayesB (ssBB), BayesC (ssBC); Bayesian Lasso (ssBL); and Bayesian ridge regression (ssBRR). For models based on mixture distributions (ssBB and ssBC), the proportions of markers having effect (π) were assumed as fixed, with respective values of 99% or 90%, or as unknown, where two approaches to estimate π were applied (ssBayesBπ and ssBayesCπ). The accuracy values found ranged from 0.550 (ssBBπ) to 0.584 (ssBCπ) and, the dispersion estimates ranged from 0.867 (ssBA) to 0.958 (ssBRR). The results indicated that Bayesian Lasso was the most suitable model for genetic evaluation of milk yield by buffaloes, considering accuracy and dispersion as criteria.
Mountain Rescue Services (MRS) are a vital link in the chain of survival when it comes to emergencies at high altitudes. Cognitive impairment in hypobaric hypoxic conditions is known, and previous studies have shown suboptimal performance of MRS members after a steep ascent. These impairments may be linked to regional cerebral oxygenation (rSO2). Therefore, this study aimed to investigate whether there are dynamics in rSO2 between “baseline” and “working” altitudes after climbing up to a potential patient.
Methods:
In this alpine proof-of-concept field study, experienced mountaineers of the Austrian MRS had to perform an active rapid ascent of 1,200 meters on foot to 3,454 meters above sea level. Near-infrared spectroscopy (NIRS) was used to measure rSO2 before and after the climb. Continuous data were compared among subgroups using Mann-Whitney-U tests, and categorical data were compared with χ2-square tests. Statistical significance was defined by two-tailed P values of <.05.
Results:
Twenty MRS members were assessed. Their rSO2 values at baseline altitude were significantly higher than at working altitude (70 [SD = 1]% versus 60 [SD = 1]%; absolute difference 10 [95% CI, 6-15]; P <.001). When assessing the single dynamics of each mountain rescuer, there was a wide variability in delta rSO2, ranging from a minimum of 0% to a maximum of 32% (mean 10 [SD = 8]%).
Conclusion:
Overall, low rSO2 values were found in mountain rescuers at high altitudes, and there were considerable interpersonal differences of changes in cerebral oxygenation after an ascent. Using rSO2 to assess performance-readiness in mountain rescuers and individual proneness to potential cognitive dysfunction or acute mountain sickness (AMS) could be further research goals.
This article explores the key standards identified by songwriters, collaborative artists, and music industry representatives in the commercial pop and Schlager sectors, along with the platform-centric myths they implicitly address. We first provide a theoretical overview of collaborative songwriting and platformisation. Through original interviews and ethnographic observations, we examine two primary platform standards: streaming and social media. We emphasise the growing significance of collaborative songwriting in the streaming era, particularly through songwriting camps, and the pervasive use of social media in creative and economic contexts. Our analysis demystifies two prevalent myths. The first myth concerns the ambiguous role of intermediaries, especially publishers, who act as new service points for efficient billing and songwriting facilitation. The second myth addresses TikTok’s success and its declining conversion rates for streaming.
In December 1936, producer Walter Futter announced that he had discovered a Sudanese princess named Kouka to play the romantic lead opposite Paul Robeson in Jericho (Thorton Freedland, 1937). “Princess Kouka” was not Sudanese, nor was she royalty, nor was she unknown. Kouka (née Nagiya Ibrahim Bilal; 1917–79) was an Egyptian actor who had been cast in supporting roles in Egyptian films. This article examines what media coverage of Kouka’s brief moment in the international limelight (1936–38) reveals about differing constructions of race across three race-conscious societies: the United Kingdom, where the film is made; the Jim Crow United States that Paul Robeson left behind; and colonial Egypt.
Drawing on focus groups conducted with musicians based in England, we discuss how musicians with backgrounds in different genres evaluate the effects of a range of music-related digital platforms on musicians and music culture. Alongside criticisms, some of them familiar from recent public debate and academic research, we identify a number of more ambivalent and even positive perspectives on the platformisation of music. We analyse the divided responses of our focus group participants under three main headings: attitudes towards music streaming platforms and record labels; attitudes towards social media and short video platforms, in particular, their use as promotional and branding mechanisms; and attitudes towards the abundance of data available to musicians from these various kinds of digital platforms. In our concluding comments, we consider the possible objection that musicians’ ambivalent and sometimes positive appraisals might represent misguided or mistaken perspectives concerning the effects of platformisation.