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We present isotopic data from mammalian megafauna from the Jirau Paleontological Site (Itapipoca, Ceará State, Brazil) and Rio Miranda (Mato Grosso do Sul State), both located in the Brazilian Intertropical Region, dating to the Mid–Late Holocene. The isotopic composition (δ13C) of eight tooth fragments was determined for the following taxa: E. laurillardi, N. platensis, T. platensis, S. populator, X. bahiense, and P. major. Results indicate that the herbivorous taxa had mixed diets, consistent with deciduous to semi deciduous forest and wooded savannah environments. S. populator likely preyed upon herbivores with mixed diets and inhabited wooded savannahs. E. laurillardi, N. platensis, and T. platensis exhibited generalist feeding behavior with a high proportion of C₃ plants in their diet, associated with the fragmentation and reduction of open environments (savannah and wooded savannah) and the concurrent expansion of forested areas during the Holocene Climatic Optimum. X. bahiense and P. major exhibited browser-type diets in Itapipoca, suggesting adaptation to the expansion of deciduous and semi deciduous forests during the Holocene Climatic Optimum. By comparing isotopic data with paleoecological, palynological, and paleobiogeographical evidence, we infer that the Intertropical Region represented one of the last environmental refuges for extinct meso- and megamammal faunas during the Holocene.
Previous publications by the authors put forward the argument that Lifelike Cellular Automata (LCAs) can be treated as a bona fide example of livingness in and of themselves, not simply a toy analogue to biological life. Traits known to be indicative of biological life – biosignatures – were identified in informational form as particular outlier traits of the ruleset for the LCA known as Conway’s Game of Life (CGOL). This publication reverses that logic, looking at a known outlier trait of CGOL – its very long-lasting evolutions – and using this to point towards temporal retention as an informational biosignature concept.
The Bermudan Archipelago supports a high-latitude subtropical coral reef ecosystem ∼1500 km from the nearest major reef system in the Tropical Western Atlantic (TWA) and Greater Caribbean. Although isolated, Bermuda has low rates of marine endemism due to regular long-distance dispersal from the TWA via the Gulf Stream Current. Nevertheless, Bermuda’s coral reef biodiversity is a reduced subset of that in the Greater Caribbean, indicating that its isolation does limit some species from dispersing or establishing persistent populations. The Alpheus armatus Rathbun, 1901 species complex is a clade of five pistol snapping shrimp species that form obligate symbioses with sea anemones from the TWA and Greater Caribbean. Although their host anemones are abundant, no members of this complex are known from Bermuda. We provide the first report of the A. armatus species complex from Bermudan coral reefs. Using molecular approaches, we identified our observed individual as Alpheus immaculatus Knowlton and Keller, 1983. Phylogenetic and species delimitation analyses indicate that our A. immaculatus sample from Bermuda does not represent a cryptic lineage nor genetically differentiated population. Instead, we interpret this species to have arrived recently as a chance long-distance dispersal event. No other A. immaculatus individuals were observed, suggesting this species has not been able to establish a long-term population in Bermuda.
Turbulent pipe flow is of substantial importance in practical applications, and it remains challenging to depict the characteristic complex multiscale dynamics by a unified theoretical framework, hindered by its inherent intermittency. Inspired by a recent study of velocity circulation in turbulent channel flows from Duan, Chen & Sreenivasan (2025 J. Fluid Mech., vol. 1009, p. R4), in this study, we investigate the statistical characteristics of velocity circulation (or equally the area integral of wall-normal vorticity) over rectangular loops in concentric cylindrical shells, parallel to the pipe wall. The statistics are implemented using direct numerical simulation data at friction Reynolds numbers of $ \textit{Re}_\tau =1057$ and $2000$. Close to the pipe wall, the circulation in the inertial range resides on space-filling unifractal sets, with the Hölder exponent smaller than Kolmogorov’s $4/3$. Away from the pipe wall, the circulation displays bifractal characteristics and the Hölder exponents for high moment orders are very close to those reported in channel flows and homogeneous isotropic turbulence. The circulation statistics are only dependent on the area enclosed by the loops, and are invariant to the loop aspect ratio, once both edge lengths of the loops are in the inertial range.
Traditional Reynolds-averaged Navier–Stokes (RANS) closures, based on the Boussinesq eddy-viscosity hypothesis and calibrated on canonical flows, often yield inaccurate predictions of both mean flow and turbulence statistics. Here, we consider flow past a circular cylinder over a range of Reynolds numbers ($3900$–$100\,000$) and Mach numbers ($0$–$0.3$), encompassing incompressible and weakly compressible regimes, with the goal of improving predictions of mean velocity and Reynolds forces. To this end, we assemble a cross-validated dataset comprising hydrodynamic particle image velocimetry (PIV) in a towing tank, aerodynamic PIV in a wind tunnel and high-fidelity spectral element direct numerical simulation and large eddy simulation. Analysis of these data reveals a universal distribution of Reynolds stresses across the parameter space, which provides the foundation for a data-driven closure. We employ physics-informed neural networks (PINNs), trained with the unclosed RANS equations, to infer the velocity field and Reynolds-stress forcing from boundary information alone. The resulting closure, embedded in a forward PINN solver and the numerical solver OpenFOAM, significantly improves RANS predictions of both mean flow and turbulence statistics relative to conventional models.
Electrohydrodynamic (EHD) instabilities at polymer–porous interfaces play a pivotal role in determining interfacial morphology, wettability and pattern formation, with implications for energy storage, diagnostics and flexible electronics. This study presents a comprehensive general linear stability analysis to examine electric-field-induced instabilities at a confined interface between a viscoelastic polymer gel and a saturated porous medium. By coupling Maxwell stresses with a modified Darcy–Brinkman–Kelvin–Voigt framework, the model captures how porous medium-moderated EHD instabilities influence both the onset and dominant instability modes. Key parameters – including the electric Rayleigh number, Darcy number, dielectric contrast and geometric filling ratio – govern the spatio-temporal features of emerging patterns. The analysis reveals a sigmoidal dependence of characteristic length and time scales on permeability, i.e. Darcy number, establishing three regimes: impermeable, transitional and highly permeable, with a shift toward shorter wavelengths. The length and time scale transitions, triggered by the solid-saturated porous medium, are further moderated by the dielectric contrast – instabilities are suppressed when the contrast is low and amplified when it is high, enabling sub-micron patterning. Geometric confinement, i.e. increasing filling ratio, further intensifies pattern length scales, suggesting the feasibility of fabricating complex ultra-fine nanoscale encapsulated porous patterns. The elasticity of the viscoelastic layer imposes a threshold for instability onset and is critical for identifying wettability transitions at the interface. This framework offers predictive insight into tuning instability modes through permeability–viscoelasticity–electrostatics interplay, laying the foundation for wettability-controlled interfaces and self-organised interfacial patterns in next-generation EHD-driven systems.
Oil contamination in estuarine and coastal ecosystems presents major challenges for environmental monitoring due to the complex mixture of organic matter from biogenic and petrogenic sources. This review synthesizes recent advances and limitations in the combined use of carbon isotopes δ13C and Δ14C to trace petroleum contamination in marine sediments. Sixteen empirical studies were analyzed to evaluate the effectiveness of this dual-isotope approach in identifying fossil carbon, estimating its degradation state, and assessing its environmental persistence. While δ13C provides insights into organic matter sources and transformation, Δ14C offers a sensitive tracer for detecting fossil carbon inputs, even in low concentrations or mixed matrices. The review highlights how their integration strengthens source attribution and enhances the resolution of hydrocarbon monitoring in dynamic coastal settings. An exploratory typology based on Δ14C and Fm is proposed to semi-quantitatively classify contamination severity and support cross-study comparisons. Despite its potential, the approach faces challenges such as high analytical costs, limited laboratory access, and a lack of methodological standardization. Future efforts should focus on integrating compound-specific isotope analysis, expanding applications in mangrove ecosystems, and refining interpretative models to improve the forensic utility of carbon isotopes in oil spill investigations. This review contributes to the systematization of isotopic methods in environmental forensics and reinforces their role in tracing petroleum-derived carbon in sensitive coastal environments.
This paper presents 56 AMS radiocarbon dates from three early medieval sites in Italy: nine from the Roman Villa of Vacone in Vacone (RI), Lazio, 29 from the Roman Villa of Selvicciola in Ischia di Castro (VT), Lazio, and 18 from the necropolis of Povegliano Veronese (VR), Veneto. These results more than double the number of previously published radiocarbon dates from early medieval Italy and are therefore a substantial contribution to the absolute chronology of early medieval cemeteries of Italy. These dates have implications for the relative dating of grave goods, grave reuse, and explaining the presence of graves with multiple individuals.
This paper investigates asymmetric shock reflection in a supersonic overexpanded jet. A theoretical model that can predict the size and shape of flow field structures such as shocks, expansion waves and sliplines is established. For symmetric Mach reflection, the current model exhibits better agreement with numerical simulation results. For asymmetric Mach reflection, the current model also shows good agreement with numerical simulation results in predicting Mach stem height. The research indicates that the Mach stem height decreases with increasing nozzle pressure ratio and nozzle exit length difference, and decreases as the nozzle exit Mach number decreases. In addition, the critical geometric condition for complete misalignment of the upper and lower slipline interference segments (i.e. when the interference between one side of the expansion wave and the slipline ends, the other side of the expansion wave has not yet begun to interfere with the slipline) is given, which increases approximately linearly with nozzle pressure ratio and decreases as the nozzle exit Mach number increases. This study provides important theoretical support for the engineering application of asymmetric shock reflection in supersonic overexpanded jet.
Many environmental, energy and industrial processes involve the flow of viscoelastic polymer solutions in three-dimensional (3-D) porous media where fluid is confined to navigate through complex pore space geometries. As polymers are transported through the tortuous pore space, elastic stresses accumulate, leading to the onset of unsteady, time-dependent flow fluctuations above a threshold flow rate. How does pore space geometry influence the development and features of this elastic instability? Here, we address this question by directly imaging polymer solution flow in microfabricated 3-D ordered porous media with precisely controlled geometries consisting of simple-cubic (SC) or body-centred cuboid (BC) arrays of spherical grains. In both cases, we find that the flow instability is generated at stagnation points arising at the contacts between grains rather than at the polar upstream/downstream grain surfaces, as is the case for flow around a single grain. The characteristics of the flow instability are strongly dependent on the unit cell geometry: in SC packings, the instability manifests through the formation of time-dependent, fluctuating 3-D eddies; whereas in BC packings, it manifests as continual fluctuating ‘wobbles’ and crossing in the flow pathlines. Despite this difference, we find that characteristics of the transition from steady to unsteady flow with increasing flow rate have commonalities across geometries. Moreover, for both packing geometries, our data indicate that extensional flow-induced polymeric stresses generated by contact-associated stagnation points are the primary contributor to the macroscopic resistance to flow across the entire medium. Altogether, our work highlights the pivotal role of inter-grain contacts – which are typically idealised as discrete points and therefore overlooked, but are inherent in most natural and engineered media – in shaping elastic instabilities in porous media.
We investigate mixing dynamics in porous media at finite times, using pore-scale lattice-Boltzmann simulations combined with Lagrangian particle tracking. We compute fluid deformation in randomly packed beds based on the moving Protean frame approach introduced by Lester et al. (2018 J. Fluid Mech. 855, 770–803). From the extracted Lagrangian kinematics, we construct a mixing model based on lamellar aggregation that well predicts the Eulerian scalar fields obtained from simulations. Our results reveal an early-time mixing regime dominated by shear-driven fluid deformation, where solute mixing arises from the random overlap of diffusive concentration elements. In this regime, mixing proceeds slowly and follows a temporal decay of concentration variance, $\sigma _c^2 \propto \textit{Pe}^{-\alpha /(2\alpha +1)} t^{-1/2}$, where $ \textit{Pe}$ is the Péclet number and $\alpha$ the exponent characterising shear deformation. This dynamic arises when the Péclet number is small relative to the ratio between the exponential-mixing and shear-deformation time scales. This analysis also demonstrates that shear-induced mixing governs the homogenisation of early-stage reactions at the fluid–solid interface in finite-size random packed beds, typically operating at moderate Péclet numbers $ \textit{Pe} =O(10^2)$.
We experimentally study a scallop-like swimmer with reciprocally flapping wings in a nearly frictionless, cohesive granular medium consisting of hydrogel spheres. Significant locomotion is found when the swimmer’s flapping frequency matches the inverse relaxation time of the material. Remarkably, the swimmer moves in the opposite direction compared with its motion in a cohesion-free granular material of hard plastic spheres. At higher or lower frequencies, we observe no motion of the swimmer, apart from a short initial transient phase. X-ray radiograms reveal that the wing motions create low-density zones, which in turn give rise to a hysteresis in drag and propulsion forces. This time-dependent effect, combined with the swimmer’s inertia, accounts for locomotion at intermediate frequencies.
This work studies the hydrothermal synthesis of zeolitic materials from three types of industrial waste (granite cutting sludge; slate cutting sludge and aggregate washing sludge), which are regarded as low-cost materials. The synthesis was carried out through acid pretreatment with aqua regia to minimize iron content, followed by alkaline melting at 600°C followed by a hydrothermal crystallization stage at 180°C for 12 h. Characterization of the three synthesized zeolite materials by X-ray fluorescence, X-ray diffraction, Fourier-transform infrared spectroscopy and scanning electron microscopy with energy-dispersive X-ray spectroscopy confirmed that the methodology used induced the formation of LOS (Losod) zeolite (Na12Al12Si12O48·xH2O) as the predominant phase in all products, regardless of the waste used as the raw material. The end-products have a significant amorphous fraction (33–43 wt.%) and a zeolitic crystalline fraction (51–66 wt.%) and are enriched in Na and Al, and they have Si/Al and Na/Al ratios ranging from 1.27 to 1.39 and from 0.58 to 1.05, respectively, characteristic of low-silica zeolites. The synthetic zeolites showed reduced CO2 adsorption capacities at room temperature (0.50–0.55 mmol g–1) compared to commercial zeolites such as 13X (3.45 mmol g–1).
Coarse-grained continuous descriptions for lipid bilayers are typically based on minimising the Helfrich energy. Such models consider the fluid properties of these structures only implicitly and have been shown to nicely reproduce equilibrium properties. Model extensions that also address the dynamics of these structures are surface (Navier–)Stokes–Helfrich models. They explicitly account for membrane viscosity. However, these models also usually treat the lipid bilayer as a homogeneous continuum, neglecting the molecular degrees of freedom of the lipids. Here, we derive refined models that consider in addition a scalar order parameter representing the molecular alignment of the lipids along the surface normal. Starting from hydrodynamic surface liquid crystal models, we obtain a hydrodynamic surface Landau–Helfrich model for asymmetric lipid bilayers and a surface Beris–Edwards model for symmetric lipid bilayers. The fully ordered case for both models leads to the known surface (Navier–)Stokes–Helfrich models. Besides more detailed continuous models for lipid bilayers, we therefore also provide an alternative derivation of surface (Navier–)Stokes–Helfrich models. The impact on the dynamics is demonstrated by numerical simulations.
To address the challenges of long voyages and the significant effects of Earth’s curvature on ocean navigation, this paper proposes, for the first time, a guidance and control strategy for great-circle routes based on Mercator projection nautical charts. First, a guidance strategy for great-circle routes is designed by combining the traditional line-of-sight (LOS) algorithm with spherical triangles. Tracking control is subsequently achieved through the integration of a closed-loop gain-scheduling algorithm. Next, the vessel’s position is transformed from a planar map to a Mercator projection nautical chart to better meet the practical needs of maritime engineering. Finally, the effectiveness of the designed guidance and control algorithm is verified through simulations. The experimental results show that the proposed guidance and control strategy can significantly enhance the stability of the vessel along the great-circle route, reduce navigation time and lower fuel consumption, demonstrating high navigation efficiency and economy.
The inner–outer interaction model (IOIM), first proposed by Marusic et al. (Science, 2010, vol. 329, pp. 193–196), has proven to be an effective turbulence model for canonical and non-canonical wall-bounded flows, where a reference velocity signal from the logarithmic region acts as the input for predicting near-wall velocity fluctuations. Its most recent iteration by Baars et al. (Phys. Rev. Fluids, 2016, vol. 1, p. 054406) further proposes a user-independent scale separation point, refining model parameters. In this study, we compared the long-perceived universal IOIM’s parameters, including the linear transfer kernel, amplitude modulation coefficients and the universal signal for a range of Reynolds and Mach numbers, where mathematical relationships between the parameters are proposed. We observed that while the universal signals exhibit a high degree of similarity, particularly near the wall, the amplitude modulation coefficients and linear transfer kernels display Reynolds and Mach number effects, where varying the reference location also causes them to exhibit significant changes. We have found transformations to collapse amplitude modulation coefficients for incompressible flows and differing reference locations, improving modelling via the IOIM across flow parameters. Despite this, compressibility effects cannot be suitably accounted for currently and remain a future challenge for the IOIM framework.
Many species of hermit crabs, such as Calcinus and Clibanarius spp., live on rocky shores in tropical and warm temperate regions. During low tide, some of these species climb out of tidal pools onto exposed rock surfaces with their shell-aperture directed upwards. However, the adaptive significance of the ‘air-exposure (AE) behaviour’ was only previously investigated in several Clibanarius species; this behaviour is hypothesised to prevent competition for shells in tide pools during low tide. Considering the lack of knowledge of Calcinus species, understanding their significance is necessary to elucidate the ecological or evolutionary and general adaptive significance of AE behaviour in hermit crabs. The AE behaviour of Ca. laevimanus, which is the dominant species in sub-tropical and tropical rocky shores, was investigated on the rocky shores of Naze, Amami, Kagoshima, Japan. The generalised linear model indicated a significant positive effect of the gastropod shell length on the occurrences of this AE behaviour. Therefore, the current result indicates that individuals with larger gastropod shells, which offer them the potential to grow to larger sizes, more frequently engage in AE behaviour during low tides. The present result supports the previous hypothesis but suggests that external factor (i.e. shell size)-dependent AE behaviour has evolved in Calcinus species, which differs from the internal factor (i.e. body size or sex)-dependent AE behaviour in Clibanarius species.