Plant-soil microbial interactions play a central role in maintaining biodiversity and coexistence in terrestrial ecosystems. However, to what extent plant-soil feedbacks (PSFs) operate in tropical Afromontane forests remains unclear. In this study, we conducted a PSF shade house experiment using six tree species exhibiting diverse life-history strategies and abundances in a sub-tropical montane forest in Nigeria. Seedlings were grown under controlled conditions in sterilised soil with or without soil inoculum collected under mature trees of each of the six species. We assessed whether conspecific tree seedlings’ performance was altered in comparison to heterospecific seedlings when grown in the soil of their adult trees. Seedling growth did not significantly differ between inocula from conspecific and heterospecific adults in five of the six species tested, indicating no evidence of PSFs. In Garcinia smeathmannii, we found a significant increase in seedling growth when grown in conspecific soil inocula. Given that no PSFs were observed in five out of six species, our study suggests that PSFs may play a limited role in the performance of some species in this Afromontane ecosystem. Nonetheless, the facilitative interaction noted in Garcinia smeathmannii indicates a nuanced ecological dynamic worth further exploration.

Epidemiologic evidence on the association between dietary choline, betaine and mortality risk remains limited, particularly among non-Western populations. We examined the association of dietary choline and betaine with all-cause mortality in Chinese adults using data from the China Health and Nutrition Survey 1991–2015. We included 9027 men and 8828 women without CVD and cancer at baseline. Dietary intake was assessed using 3-day 24-hour dietary recalls and household food inventories. Death was ascertained through household surveys in each wave. Time-dependent Cox proportional hazards regression models estimated multivariable-adjusted hazard ratios (HRs) and 95 % CIs. During a median follow-up of 9·1 years, 891 men and 687 women were deceased. Higher total choline intake was associated with lower all-cause mortality in both men (HRQ5 v. Q1 = 0·58 (95 % CI: 0·45, 0·74)) and women (HRQ5 v. Q1 = 0·59 (95 % CI: 0·44, 0·78)). The dose–response curve were reverse J-shaped in men and L-shaped in women (both P-nonlinear ≤ 0·005). Similarly, fat-soluble choline intake was inversely associated with mortality in both men (HRQ5 v. Q1 = 0·59 (95 % CI: 0·46, 0·75)) and women (HRQ5 v. Q1 = 0·53 (95 % CI: 0·40, 0·70)), showing reverse J-shaped patterns (both P-nonlinear < 0·001). A J-shaped association between water-soluble choline and mortality was observed in women (P-nonlinear < 0·001), but a null association was found in men. Betaine intake was not associated with all-cause mortality in either sex. Our findings suggest that adequate choline intake is linked to reduced all-cause mortality in Chinese adults with predominantly plant-based diets.

The RDA for dietary protein is likely insufficient for individuals with cystic fibrosis (CF). This study sought to characterise protein intake and diet quality in adults with cystic fibrosis (awCF), before and after elexacaftor/tezacaftor/ivacaftor (ETI) therapy, compared with healthy controls. Dietary intake was assessed by diet diary in awCF at baseline (BL, n 40) and at follow-up > 3 months post ETI therapy (follow-up (FUP), n 40) and in age-matched healthy controls (CON, n 80) free from known disease at a single time point. Protein intake dose and daily distribution, protein quality, protein source and overall diet quality were calculated for each participant. Both CON (1·39 (sd 0·47) g·kg–1·day–1) and CF (BL: 1·44 (sd 0·52) g·kg–1·day–1, FUP: 1·12 (sd 0·32) g·kg–1·day–1) had a higher mean daily protein intake than the protein RDA of 0·75g·kg–1·day–1. There was a significant reduction in daily protein intake in the CF group at FUP (P = 0·0003, d = 0·73), with levels below the alternative suggested dietary intake of ≥ 1·2 g·kg–1·day–1. There were no sex differences or noticeable effects on protein quality or source following the commencement of ETI therapy when compared with CON (all P > 0·05), although overall diet quality decreased between time points (P = 0·027, d = 0·57). The observed reduction in daily protein intake in the present cohort emphasises the importance of ensuring appropriate dietary protein intake to promote healthy ageing in adults with CF. More research is needed to evidence base dietary protein requirements in this at-risk population.

Diet quality has been linked to socio-economic status. However, evidence within rural and regional populations is lacking. This cross-sectional study examined the relationship between diet quality and socio-economic position in adults living in rural and regional areas of Australia. The Australian Recommended Food Score (ARFS; range 0–73) measured diet quality (total and subscale scores). Area-level socio-economic position was determined by postcode-linked socio-economic index for areas (SEIFA), Index of Relative Social Advantage and Disadvantage scores, stratified into quintiles. The mean total ARFS (34·7; sd = 9·1; n 836) was classified as ‘getting there’. Findings showed significantly lower mean total ARFS between SEIFA quintile 1 (1 = lowest; mean total ARFS = 30·4; sd = 10·2; categorised as ‘needs work’) compared with all other SEIFA quintiles (F (44 831) = 8·44, P ≤ 0·001). Linear regression, adjusting for age, sex, income, education, employment status and household composition demonstrated significantly lower overall diet quality for SEIFA quintile 1 compared with SEIFA quintile 3 (B = –3·9; 95 % CI (–6·2, −1·5); P < 0·001) and lower subscale scores for vegetables (B = –1·6; 95 % CI (–2·7, −0·6); P = 0·003), fruit (B = –0·9; 95 % CI (–1·6, −0·1); P = 0·018) and grains (B = –0·6; 95 % CI (–1·3, −0·0); P = 0·050). After adjusting for individual confounders of diet quality, results indicate that lower area-level socio-economic position remained associated with poorer diet quality in this sample of rural and regional Australian adults. This suggests that broader social and environmental factors unique to these areas may impact diet quality and amplify individual barriers to achieving a healthy diet.

Maternal ability (milk and environment) in beef cattle is one of the most important traits influencing weaning weight of the calf, but amount and composition of milk produced by the dam is difficult to measure. The assessment of polymorphisms of candidate genes related to milk composition could indirectly enlighten this perspective. In the present study, the frequency of αs1-casein 1175AG and g26181A>G; κ-casein 13068 (CSN3 AB1) and 13104 (CSN3 AB2) loci, β-lactoglobulin variants 3984GA and 5263CT, Diacyl glycerol transferase K232A, and Stearoyl CoA desaturase A702G and A762G polymorphisms were estimated in Gray and Red Brahman, Nellore, Guzerat, Gir, Indubrazil, and Sardo Negro registered sires from Mexico. Most of the documented favourable alleles were found in low frequency in most of the evaluated breeds, except for β-lactoglobulin, in which the presence of favourable alleles might represent an opportunity for marker-assisted introgression. The relevance of the findings for each variation and implications from the outcomes are discussed.

Neurotransmitter release via synaptic vesicle fusion with the plasma membrane is driven by SNARE proteins (Synaptobrevin, Syntaxin, and SNAP-25) and accessory proteins (Synaptotagmin, Complexin, Munc13, and Munc18). While extensively studied experimentally, the precise mechanisms and dynamics remain elusive due to spatiotemporal limitations. Molecular dynamics (MD) simulations—both all-atom (AA) and coarse-grained (CG)—bridge these gaps by capturing fusion dynamics beyond experimental resolution. This review explores the use of these simulations in understanding SNARE-mediated membrane fusion and its regulation by Synaptotagmin and Complexin. We first examine two competing hypotheses regarding the driving force of fusion: (1) SNARE zippering transducing energy through rigid juxtamembrane domains (JMDs) and (2) SNAREs generating entropic forces via flexible JMDs. Despite different origins of forces, the conserved fusion pathway – from membrane adhesion to stalk and fusion pore (FP) formation – emerges across models. We also highlight the critical role of SNARE transmembrane domains (TMDs) and their regulation by post-translational modifications like palmitoylation in fast fusion. Further, we review Ca²⁺-dependent interactions of Synaptotagmin’s C2 domains with lipids and SNAREs at the primary and tripartite interfaces, and how these interactions regulate fusion timing. Complexin’s role in clamping spontaneous fusion while facilitating evoked release via its central and accessory helices is also discussed. We present a case study leveraging AA and CG simulations to investigate ion selectivity in FPs, balancing timescale and accuracy. We conclude with the limitations in current simulations and using AI tools to construct complete fusion machinery and explore isoform-specific functions in fusion machinery.

The whitefly Bemisia tabaci (Hemiptera: Aleyrodidae) is economically one of the most threatening pests in tomato cultivation, which not only causes direct damage but also transmits many viruses. Breeding whitefly-resistant tomato varieties is a promising and environmentally friendly method to control whitefly populations in the field. Accumulating evidence from tomato and other model systems demonstrates that flavonoids contribute to plant resistance to herbivorous insects. Previously, we found that high flavonoid-producing tomato line deterred whitefly oviposition and settling behaviours, and was more resistant to whiteflies compared to the near-isogenic low flavonoid-producing tomato line. The objective of the current work is to describe in detail different aspects of the interaction between the whitefly and two tomato lines, including biochemical processes involved. Electrical penetration graph recordings showed that high flavonoid-producing tomato reduced whitefly probing and phloem-feeding efficiency. We also studied constitutive and induced plant defence responses and found that whitefly induced stronger reactive oxygen species accumulation through NADPH oxidase in high flavonoid-producing tomato than in low flavonoid-producing tomato. Moreover, whitefly feeding induced the expression of callose synthase genes and resulted in callose deposition in the sieve elements in high flavonoid-producing tomato but not in low flavonoid-producing tomato. As a consequence, whitefly feeding on high flavonoid-producing tomato significantly decreased uptake of phloem and reduced its performance when compared to low flavonoid-producing tomato. These results indicate that high flavonoid-producing tomato provides phloem-based resistance against whitefly infestation and that the breeding of such resistance in new varieties could enhance whitefly management.

The 13 species of hornbills occurring in Indonesia have been studied to varying degrees, but a comprehensive identification of knowledge gaps is still lacking. We conducted a systematic review of the literature to identify the species and topics that require further investigation. We searched the literature using ScienceDirect, Google Scholar, Neliti, ResearchGate, and the Google search engine for all publications from 1970 to 2023. Publications were grouped under 15 conservation and ecological topics related to hornbills. Our review included 183 publications, comprising journal articles (56.3%), theses/dissertations (23.5%), conference papers (18.6%), and small portions of books and book sections (1.6%). Almost all the literature (72%) had been published after 2013, while the remaining (28%) was published before this period. The need for further studies across all research topics was identified for several species, including the Sumba Hornbill Rhyticeros everetti, Sulawesi Hornbill Rhabdotorrhinus exharratus, and Papuan Hornbill Rhyticeros plicatus. We also underscore the need for investigation into seed dispersal, ecotourism, and research methods across all hornbill species. Enhancing research and drawing public attention to Indonesia’s hornbills should increase their popularity and may serve to increase financial resources. This is the first systematic review of hornbill research for Indonesia and provides important information to determine future hornbill studies in the country.

Water hyacinth is an invasive aquatic plant that has been associated with major negative economic and ecological impacts in water systems worldwide, including Rwanda, since its establishment in the country in the 1960s. While biological control is considered the most sustainable management method, the success of biocontrol agents depends on various abiotic factors, with temperature being critical. This study assessed the suitability of potential water hyacinth biocontrol agents such as: Neochetina weevils, Megamelus scutellaris Berg (Hemiptera: Delphacidae), and Cornops aquaticum Bruner (Orthoptera: Acrididae) for regions with a temperate climate by testing their thermal boundaries. Using thermal physiology limits and CLIMEX modelling, we found that Neochetina eichhorniae Warner and N. bruchi Hustache (Coleoptera: Curculionidae) had lower thermal minimums (CTmin) of 2.4°C and 2.6°C, respectively, compared to Megamelus scutellaris (4.7°C) and Cornops aquaticum (6.2°C). CLIMEX modelling predicted the suitability of Neochetina weevils and C. aquaticum across Rwanda, while M. scutellaris appeared unsuitable for the colder northern regions of the country but appropriate for the central and eastern regions. These findings suggests that the historical failure of Neochetina weevils introduced to Rwandan water bodies in 2000 was not due to temperature extremes. Rather, other factors such as release numbers or water quality may have played a role. This study provides crucial information for future biocontrol efforts in Rwanda and similar temperate regions, highlighting the importance of pre-release thermal tolerance assessments and climate modelling to predict biocontrol agent establishment and efficacy.

Dietary patterns enriched in fermentable fibre (such as inulin) and inorganic nitrate are linked to cardiovascular benefits, possibly mediated by gut microbiota-derived bioactive compounds including short-chain fatty acids (SCFAs) and nitric oxide (NO). However, the potential synergistic effects remain unclear. We conducted a randomised, double-blind, crossover study to investigate the acute effects of inulin (15 g; INU), nitrate (400 mg; NO3−), and their combination (INU + NO3−) on plasma nitrate and nitrite levels, SCFAs, and blood pressure (BP) in 20 adults. Plasma nitrate and nitrite were significantly elevated following INU + NO3− and NO3− compared to INU (p < 0.001). Plasma SCFAs were increased after INU + NO3− and INU, but the incremental AUC was not statistically significant, likely due to large inter-individual variability. No significant main effects were observed on BP; however, inverse correlations were identified between peak plasma nitrite and diastolic BP (rs = −0.61, p = 0.004) and mean arterial pressure (MAP) (rs = −0.59, p = 0.005) following INU + NO3−. Peak nitrate concentrations were inversely correlated with diastolic BP following NO3− (rs = −0.47, p = 0.004). Co-supplementation with inulin and nitrate did not enhance plasma nitrate/nitrite or BP beyond nitrate alone but modulated SCFA profiles, suggesting potential interactions between fibre fermentation and nitrate metabolism for cardiovascular health.

Legume lectins represent a broad class of environmental toxicants that bind to cell surface glycoproteins. Raw red kidney beans (RRKB), a widely consumed common source of dietary protein, are rich in the lectin phytohemagglutinin (PHA). Consumption of improperly cooked (which may require overnight presoaking and boiling at least at 100°C for 45 min) red kidney beans causes severe gastrointestinal symptoms. Since the relationship between lectin toxicity and the cellular chaperone machinery remains unknown, the study aimed to determine the effects of heat-denatured PHA on epithelial barrier function and heat shock protein 70 (HSP70) expression and its function as a molecular chaperone in PHA-treated Caco-2 cells and animals. Twelve male Sprague-Dawley rats were randomised to an ad libitum diet of either standard rat chow or chow containing 26% crude red kidney beans. We measured HSP70 and heat shock factor 1 gene expressions in the small intestine and HSP70 protein expression in Caco-2 cells. In Caco-2 cells, luciferase activity was measured to investigate protein folding. Fluorescein-5-isothiocyanate (FITC)-labelled lectin was used to study its intracellular uptake by Caco-2 cells. PHA lectin reduced transepithelial electrical resistance in Caco-2 cells. FITC-labelled PHA entered Caco-2 cells within 3 h of treatment. PHA treatment significantly reduced HSP70 levels and luciferase activity in Caco-2 cells, which was prevented by HSP70 overexpression. In rats fed RRKB chow consisting of legume lectins, we found reduced levels of HSP70 and heat shock factor 1. These observations suggest that lectins counter the protective function of HSP70 on intestinal barrier function.

We investigated the effects of maternal vitamin and mineral supplementation throughout gestation on gene expression in the jejunal mucosa of neonatal calves. Crossbred Angus heifers (n = 14) were estrus synchronized, bred to female-sexed semen, and randomly assigned to a basal diet (Control, CON; n = 7) or the basal diet plus vitamin and mineral supplement (Treatment, VTM; n = 7). After parturition, calves were removed from their dams before suckling, fed colostrum replacer, and euthanized 30 h after the first feeding. A subsample of the mucosa of the mid-jejunum was collected, and total RNA was isolated. Gene expression was measured using RNA-Seq, and differentially expressed genes (DEGs) were identified using DESeq2. We identified 528 DEGs from the jejunal mucosa between the VTM and CON calves (P ≤ 0.05 and |log2FC| ≥ 0.5). The DEGs were associated with nutrient transport, lipid metabolism, and immune-related biological processes and pathways. Interestingly, genes underlying the complement and coagulation cascades were mostly downregulated in calves born to VTM dams. On the other hand, the cytokine-cytokine receptor interaction KEGG pathway showed most genes upregulated (LIFR, KDR, TNFRSF4, TNFSF18, FLT1, and TNFRSF12A). Our results show that vitamin and mineral supplementation throughout gestation affects genes underlying tissue structure, nutrient transport and metabolism, and immune system pathways in neonates. The implications of such changes and the long-term outcomes on herd health and performance warrant further research.

Spousal age differences are highest in Sub-Saharan Africa, where trends in age at first marriage indicate an increase for both men and women. However, the net outcome for spousal age difference is difficult to predict without explicit analysis of these distributions. This study examines differentials in spousal age for women in first union. Further, it examines differences within population sub-groups and across countries, focusing on the influence of age at marriage and educational attainment. The analysis pools 144 survey datasets from the Demographic and Health Surveys (DHS) and Multiple Indicator Cluster Surveys (MICS) conducted between 1980 and 2020 in 34 countries. OLS regression analysis was used to predict the spousal age difference at marriage with five-year cohorts used to study time trends. The analysis finds a decline of 1.7 years in the spousal age difference at first marriage between the earliest (1980–1984) and most recent (2014–2019), indicating that there has been minimal reduction in age at first marriage over four decades, despite rising ages at marriage for females. Increasing age at first marriage and educational attainment of women partly explain the decline observed over time.

The aim of this study was to describe how the detection of protozoan and helminth parasites has been affected by the introduction of polymerase chain reaction (PCR) and changes in test algorithms. We extracted data about faecal samples tested for parasites (n = 114839) at five Norwegian clinical microbiology laboratories. Samples were classified into prePCR or postPCR depending on whether they were submitted before or after the introduction of PCR, and into diagnostic episodes (n = 99320). The number of diagnostic episodes increased 3.7-fold from prePCR to postPCR. Giardia positive episodes doubled, the positivity rate decreased from 2.0% to 1.3%. Cryptosporidium was hardly detected prePCR and increased to a positivity rate of 1.2%. Entamoeba histolytica was rarely found. Episodes examined for helminths decreased 51%, the number of positive episodes decreased 34%. Samples from immigrants were more likely to be positive for Giardia, E. histolytica, or helminths and less likely to be Cryptosporidium positive. During the COVID-19 pandemic, the number of Giardia and helminth-positive episodes decreased. Cryptosporidium-positive episodes remained unchanged. The implementation of multiplex PCR for protozoa led to a doubling of Giardia cases and a better test for Cryptosporidium. Fewer microscopy examinations raise concerns that helminth infections may be overlooked.

The two-spined deepwater cardinalfish, Epigonus bispinosus Okamoto & Gon 2018 is documented for the first time from the Arabian Sea (n= 2) (137.4–151.1 mm SL). The specimens were caught by a demersal shrimp trawl operated along the Kerala coast at a depth of 250–420 m. This species was previously recorded in South Africa and northern Madagascar regions. The present study also provides the first molecular information on E. bispinosus based on mitochondrial cytochrome oxidase subunit I and 16S ribosomal RNA genes. Detailed taxonomic identification, and molecular and phylogenetic analysis are described.

Integrating trees and shrubs into agroecosystems increases soil nutrients and organic matter, which helps in the recovery of degraded tropical soils. However, selecting the most appropriate tree and shrub species as sources of nutrients and organic matter requires knowledge of their productive potential. The study aimed to evaluate litter productivity and nutrient input to the soil of three tropical silvopastoral fodder bank species. Litter input from Tithonia diversifolia, Leucaena leucocephala, and Moringa oleifera was quantified monthly for one year. We found that the litterfall amount was greatest (p < 0.05) from T. diversifolia (1.81 t ha–1 yr–1) compared to L. leucocephala (1.26 t ha–1 yr–1) and M. oleifera (118 t ha–1 yr–1). Indeed, T. diversifolia had a greater (p < 0.05) input of biomass from leaves (1.36 t ha–1 yr–1) compared to L. leucocephala (0.901 t ha–1 yr–1) and M. oleifera (0.869 t ha–1 yr–1). The highest biomass input (p < 0.05) occurred in September to the three species, T. diversifolia (0.516 t ha–1), L. leucocephala (0.243 t ha–1), and M. oleifera (0.233 t ha–1), and the lowest biomass input occurred in March. Furthermore, the greatest annual input of carbon (0.687 t ha–1 yr–1), nitrogen (0.030 t ha–1 yr–1), and phosphorus (0.006 t ha–1 yr–1) to the soil originated from T. diversifolia. Our study showed that T. diversifolia had the greatest capacity to produce biomass that potentially can reintroduce nutrients and contribute to the recovery process of degraded soil due to its high litter productivity, besides, a combination of these three species also improves soil nutrients in fodder banks when do not hauling forage for livestock, contributing to sustainability of system.