The aim of this study was to evaluate the antifungal spectrum of activity, synergy, and mode of action of carboxy-terminally amidated antimicrobial peptides (AMPs) derived from tachyplesin-I (T-I) from the horseshoe crab Tachypleus tridentatus and a lysine-rich analogue of magainin-2 (MSI-94) from the clawed frog Xenopus laevis. In vitro antimicrobial tests against 17 fungal strains demonstrated that the modified AMPs exhibited broad antifungal activity, particularly against filamentous fungi and yeasts relevant to aquaculture and agriculture. Additive antimicrobial activity was observed with the combination of T-I and MSI-94 against Candida albicans and Rhodotorula mucilaginosa, indicating an enhancement of their antiyeast properties. Furthermore, we found that both peptides target the fungal cell surface, increasing membrane permeability and leading to cell death. Overall, our findings highlight the biotechnological potential of aquatic AMPs in developing novel antifungal therapeutics applicable across various fields.

The extracellular matrices, such as the haemolymph, in insects are at the centre of most physiological processes and are protected from oxidative stress by the extracellular antioxidant enzymes. In this study, we identified two secreted superoxide dismutase genes (PxSOD3 and PxSOD5) and investigated the oxidative stress induced by chlorpyrifos (CPF) in the aquatic insect Protohermes xanthodes (Megaloptera: Corydalidae). PxSOD3 and PxSOD5 contain the signal peptides at the N-terminus. Structure analysis revealed that PxSOD3 and PxSOD5 contain the conserved CuZn-SOD domain, which is mainly composed of β-sheets and has conserved copper and zinc binding sites. Both PxSOD3 and PxSOD5 are predicted to be soluble proteins located in the extracellular space. After exposure to different concentrations of sublethal CPF, MDA content in P. xanthodes larvae were increased in a dose-dependent manner; SOD and CAT activities were also higher in CPF-treated groups than that in the no CPF control, indicating that sublethal CPF induces oxidative stress in P. xanthodes larvae. Furthermore, PxSOD3 and PxSOD5 expression levels and haemolymph SOD activity in the larvae were downregulated by sublethal CPF at different concentrations. Our results suggest that the PxSOD3 and PxSOD5 are putative extracellular antioxidant enzymes that may play a role in maintaining the oxidative balance in the extracellular space. Sublethal CPF may induce oxidative stress in the extracellular space of P. xanthodes by reducing the gene expression and catalytic activity of extracellular SODs.

Case-control studies can provide attribution estimates of the likely sources of zoonotic pathogens. We applied a meta-analytical model within a Bayesian estimation framework to pool population attributable fractions (PAFs) from European case-control studies of sporadic campylobacteriosis and salmonellosis. The input data were obtained from two existing systematic reviews, supplemented with additional literature searches, covering the period 2000–2021. In total, 12 studies on Campylobacter providing data for 180 PAFs referring to 5983 cases and 13213 controls, and five studies on Salmonella providing data for 75 PAFs referring to 2908 cases and 5913 controls, were included. All these studies were conducted in Western or Northern European countries. Both pathogens were estimated as being predominantly linked to food- and waterborne transmission, which explained nearly half of the cases, with Campylobacter being mainly attributable to poultry (meat), and Salmonella to poultry (eggs and meat) and pig (meat), as specific foodborne exposures. When also considering contact with animals, around 60% of cases could be explained by the larger group of zoonotic transmission pathways. While environmental transmission was also sizeable (around 10%), about a quarter of cases could be explained by factors such as travel, underlying diseases/medicine use, person-to-person transmission and occupational exposure.

Polyploidization is known to cause changes in the ploidy levels of plant somatic cells that affect the morphological, physiological and chemical composition. The aim of this research was to investigate the effects of tetraploidization in olive. To do this, several characteristics of 1-year-old shoots of two olive genotypes were compared: the diploid cultivar Leccino (L), and its tetraploid mutant Leccino Compact (LC), considered a slow-growing genotype. LC differed significantly from L in the morphological characteristics, with higher values of diameter, dry mass and volume of the stem (46%, 103%, 102%, respectively), and higher area, mass and volume of the individual leaf (43%, 66%, 73%, respectively). LC also had thicker, longer and wider leaves (30%, 10%, 34%, respectively) and significantly lower leaf density (7%) and lower specific leaf area, leaf mass ratio and leaf area ratio (17%, 4%, 18%, respectively). Internode length and stem density were not significantly different. The results allowed us to thoroughly characterize the effects of tetraploidy on 1-year-old shoots in olive, and also suggest that the slow growth of LC is due to its lower leaf area per unit of total biomass, which reduces leaf area production and, consequently, light interception, resource availability and tree growth. These results will be useful for genetic improvement programmes and for planning further exploitation of tetraploidy in horticulture.

The aim of this study was to estimate genetic parameters for dairy traits (daily milk yield (DMY), daily milk fat yield (DMFY), and daily milk protein yield (DMPY)) and somatic cell score (SCS) of Iranian Murciano-Granadina goats. The analysis involved 99,839 records from 4,307 does, spanning from 2015 to 2021. A single-trait animal model was used to obtain estimates of genetic and residual (co)variances and heritabilities. In the model, both fixed effects (birth year, parity, litter size, age dam (day)) and random effects (additive animal genetic effects) were considered. R software was used to evaluate environmental effects, assess the model, examine genetic and phenotypic trends, and determine genetic-environmental correlations. Additionally, variance components and heritability were estimated using the Gibbs1f90 software. The heritability of dairy traits and SCS trait, namely DMY, DMFY, DMPY, and SCS, was estimated to be 0.244, 0.133, 0.188, and 0.023, respectively. The genetic correlations between DMY and DMFY, DMY and DMPY, DMY and somatic cell score (SCS), DMFY and DMPY, DMFY and SCS, and DMPY and SCS were 0.07, 0.16, 0.42, –0.08, –0.005, and 0.04, respectively. The study found moderate heritability for milk yield traits and low heritability for somatic cell score. Genetic correlations varied, with the strongest correlation between daily milk yield and somatic cell score, indicating useful genetic linkages for selective breeding.

SIGIRR, also known as the single immunoglobulin interleukin-1 receptor (IL-1R)-related molecule, is a member of the IL-1 receptor superfamily and is believed to play a pivotal role in inflammation and anti-inflammatory regulation within the body. Studies have shown that SIGIRR expression is associated with autoimmunity, inflammatory disorders, graft rejection, viral infection, thrombosis and tumour progression. Due to its unique structure and function, SIGIRR is commonly referred to as an ‘orphan receptor’, with IL-37 being the only confirmed ligand molecule for SIGIRR to date. The primary mechanism through which SIGIRR exerts its anti-inflammatory regulatory effect involves the negative modulation of the Toll-like receptor-IL-1R (TLR-IL-1R) signalling pathway. TLR-IL-1R signalling plays critical roles in immune responses triggered by microbial invasion and alterations in the tumour immune microenvironment. This article provides an overview of research findings on SIGIRR as an orphan receptor and its regulatory role in maintaining a delicate balance between natural immune activation and uncontrolled inflammatory processes under pathological conditions.

Enlist E3® soybean is resistant to 2,4-D, glyphosate, and glufosinate, allowing postemergence applications of these herbicides sequentially or as tank mixes. The objectives of this experiment were to evaluate the effect of postemergence herbicide application timing and sequence with or without a preemergence application of micro-encapsulated acetochlor on waterhemp and common lambsquarters control, soybean yield, and economic returns. Field experiments were conducted in Rosemount and Franklin, Minnesota, in 2021 and 2022. Site, herbicide application timing, and sequence influenced weed control, yield, and profitability. In Rosemount, preemergence followed by (fb) two-pass postemergence programs, including 2,4-D + glyphosate applied at mid-postemergence with or without S-metolachlor, resulted in ≥95% waterhemp control at 28 d after late postemergence application. In Franklin, where weed density was lower, two-pass postemergence programs, regardless of preemergence application that included at least one application of 2,4-D + glyphosate (with or without S-metolachlor), provided ≥97% control of waterhemp and common lambsquarters at 28 d after late postemergence. The level of control was comparable to that of a preemergence herbicide fb a mid-postemergence application of 2,4-D + glyphosate + S-metolachlor at that site. In Rosemount, including acetochlor as the preemergence herbicide in the preemergence fb postemergence programs improved soybean yield by 32% and partial returns by US$384.50 ha−1 compared to postemergence herbicides–only programs. In contrast, the preemergence application did not affect yield or profitability in Franklin. The highest soybean yield (2,925.7 kg ha−1) in Rosemount resulted after glufosinate was applied early postemergence fb 2,4-D + glyphosate applied mid-postemergence. This yield was comparable to that of glufosinate applied early postemergence fb 2,4-D + glyphosate + S-metolachlor applied mid-postemergence and the two-pass glufosinate (early postemergence fb mid-postemergence) program, highlighting the importance of early season weed control. In Franklin, 2,4-D + glyphosate + S-metolachlor (applied mid-postemergence) fb glufosinate (applied late postemergence) provided a yield that was similar to the aforementioned programs at that site.

This study was designed to explore changes in soil bulk density (BD), soil organic carbon (SOC) content, SOC stocks, and soil labile organic carbon (C) fractions after 5 years of soil tillage management under the double-cropping rice system in southern of China. The experiment included four soil tillage treatments: rotary tillage with all crop residues removed as a control (RTO); conventional tillage with crop residues incorporation (CT); rotary tillage with crop residues incorporation (RT); and no-tillage with crop residues retention. Our results revealed that soil tillage combined with crop residue incorporation (CT and RT) significantly decreased BD at 0–20 cm soil layer compared to RTO treatment. SOC content and stocks were increased with the application of crop residues. Compared with RTO treatment, SOC content and stocks were increased by 16.8% and 9.8% in CT treatment, respectively. Soil non-labile C content and proportion of labile C were increased due to crop residue incorporation. Compared with RTO treatment, soil proportion of C mineralisation (Cmin), permanganate oxidisable C (KMnO4), particulate organic C (POC), and microbial biomass C (MBC) was increased by 196.1%, 41.4%, 31.4%, and 17.1% under CT treatment, respectively. These results were confirmed by the carbon management index, which was significantly increased under soil tillage with crop residue incorporation. Here, we demonstrated that soil tillage and crop residue incorporation can increase the pool of stable C at surface soil layer while increasing labile C content and proportion. In conclusion, conventional or rotary tillage combined with crop residue incorporation is a soil management able to improve nutrient cycling and soil quality in paddy fields in southern China.

Monkeypox (mpox) has re-emerged as global public health concern including in several non-endemic countries. This study aims to characterize monkeypox virus (MPXV) genomes in Indonesia, to explore viral evolution and transmission. Genomic analysis was conducted on 53 isolates from Indonesian mpox patients between 2023 and 2024. All sequences belonged to Clade IIb, with identified sub-clades including A.1.1, B.1, B.1.3, and C.1 – of which C.1 became dominant during this period. Out of 87 mpox-confirmed cases, 60.9% (53/87) were successfully sequenced and submitted to the Global Initiative on Sharing All Influenza Data (GISAID). The majority of cases in Indonesia occurred among males (95.4%), men who have sex with men (59.8%), and people living with HIV/AIDS (71.3%). Notably, a large portion of cases had no travel history, suggesting local transmission. Initially, only clade IIb (B.1) was detected in October 2022. By August 2023, lineage diversity had increased, with B.1.3 and C.1 emerging as the predominant sub-clades. A time–calibrated phylogenetic tree revealed genetic relatedness and shared ancestry within clade IIb. Integrating genomic and epidemiological data offers valuable insights to improve mpox surveillance and public health response in Indonesia and the broader region

This study is the first to investigate the intra- and interspecific differences in otolith’s length (Lo), width (Wo), perimeter (Po), area (Ao), and mass asymmetry (OMA) in the pelagic Ethmalosa fimbriata, demersal Galeoides decadactylus, and benthic Cynoglossus monodi collected from the Lebe fishing site located along the Komo Estuary, central-western Gabon. The objectives were to (1) verify whether or not otolith morphometry differs in relation to living depth levels of the three species, (2) examine fluctuating asymmetry (FA) in the otolith morphometry, and (3) assess the relationship between OMA and total length (TL). A significant asymmetry was found in Lo, Wo, Po, and Ao between the left and right otoliths within and among males and females at the intraspecific level and only in Wo and Ao at the interspecific level due to FA. At the intraspecies level, a significant OMA was only observed within females of G. decadactylus and C. monodi and males and females of E. fimbriata and C. monodi. Moreover, a significant relationship between OMA and TL was detected only among males and females within the pelagic E. fimbriata and the demersal G. decadactylus, and no significant relationship was found at the interspecific level. As a result, significant differences in Wo and Ao and non-significant differences in OMA were recorded between the three species based on variations in living depth levels. These results highlight the importance of using otolith morphometry and OMA analyses to address the relationship between fish species and their living depth levels.

Dengue, the most prevalent urban arbovirus in the world, has triggered recurrent epidemics in Rio de Janeiro, Brazil, since the 1980s. This study aimed to describe the spatial–temporal patterns of dengue spread during the epidemic years of 2002, 2008, 2011, 2012, 2013, and 2024 in Rio de Janeiro. This is an ecological study using secondary data on notified confirmed dengue cases aggregated by neighbourhood. The incidence rates were estimated via the local empirical Bayes method. The local spatial autocorrelation indicators assessed incidence clusters, and the monthly geographic trajectory was outlined for each year. The results revealed changes in the spatial distribution of dengue over time, with clusters of high incidences predominating in the northern and central neighbourhoods in 2002 and 2008, and in the western zone in 2011, 2012, and 2013. In 2024, the distribution was predominant throughout the city, with emphasis in the central and western zones. The monthly geographic centre of dengue cases shifted from the west to the north during the peak of the epidemic. These results highlight the heterogeneous nature of dengue transmission in Rio de Janeiro. The incorporation of spatial and temporal analyses in epidemiological studies can enhance targeted and localized dengue control strategies.

The use of camera traps in wildlife conservation and ecological research is a popular method of data capture due in large part to the perceived low interference levels for the animals being studied. However, evidence exists that some species alter their behaviour when exposed to this technology. The primary aim of this study was to address whether researchers working with this technology in the ecology and forestry fields are making considerations for the possible impacts of cameras on animal behaviour. A secondary aim was to investigate how the use of this technology is framed in recent publications. In this rapid systematic literature review, we conducted a search on Web of Science and we identified 267 papers published in the last five years, in the fields of ecology and forestry, that met our inclusion criteria. We screened the studies for mentions of the impact of camera traps on the welfare of wildlife. Surprisingly, only 7.5% of the papers considered the possible animal welfare impacts of camera use on the wildlife species of interest in their study, with most comparing it to invasive methods and therefore framing this technology positively. We strongly encourage researchers working in this field to consider the impact of this technology on the specific species being studied. Whilst we recognise that the use of camera traps avoids direct handling of the animals, the short- and long-term effects of using this technology should not be ignored and should, at a minimum, be acknowledged in the limitations.