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The TRiC/CCT complex assists in the folding of approximately 10% of cytosolic proteins through an ATP-driven conformational cycle, playing a crucial role in maintaining protein homeostasis. Despite our understanding of ATP-driven TRiC ring closing and substrate folding, the process and mechanisms underlying TRiC ring-opening and substrate release remain largely unexplored. In this study, by determining an ensemble of cryo-EM structures of yeast TRiC in the presence of ADP, including three intermediate transition states, we present a comprehensive picture of the TRiC ring-opening process. During this process, CCT3 detects the loss of γ-phosphate and initiates with the dynamics of its apical protrusion, and expands to the outward leaning of the consecutive CCT6/8/7/5 subunits. This is followed by significant movements of CCT2, CCT4, and especially CCT1 subunits, resulting in the opening of the TRiC rings. We also observed an unforeseen temporary separation between the two rings in the CCT2 side, coordinating the release of the originally locked CCT4 N-terminus, which potentially participates in the ring-opening process. Collectively, our study reveals a stepwise TRiC ring-opening mechanism, provides a comprehensive view of the TRiC conformational landscape, and sheds lights on its subunit specificity in sensing nucleotide status and substrate release. Our findings deepen our understanding of protein folding assisted by TRiC and may inspire new strategies for the diagnosis and treatment of related diseases.
This study demonstrates a kilowatt-level, spectrum-programmable, multi-wavelength fiber laser (MWFL) with wavelength, interval and intensity tunability. The central wavelength tuning range is 1060–1095 nm and the tunable number is controllable from 1 to 5. The wavelength interval can be tuned from 6 to 32 nm and the intensity of each channel can be adjusted independently. Maximum output power up to approximately 1100 W has been achieved by master oscillator power amplifier structures. We also investigate the wavelength evolution experimentally considering the difference of gain competition, which may give a primary reference for kW-level high-power MWFL spectral manipulation. To the best of our knowledge, this is the highest output power ever reported for a programmable MWFL. Benefiting from its high power and flexible spectral manipulability, the proposed MWFL has great potential in versatile applications such as nonlinear frequency conversion and spectroscopy.
Large-aperture gratings have significant applications in inertial confinement fusion, immersion lithography manufacturing and astronomical observation. Currently, it is challenging and expensive to manufacture sizable monolithic gratings. Therefore, tiled multiple small-aperture gratings are preferred. In this study, the impact of seam phase discontinuity on the modulation of the laser beam field was explored based on the measurement results of the Shenguang-II laser large-aperture multi-exposure-tiled grating. An innovative method for accurately calculating the phase jump of multi-exposure-tiled grating seams was proposed. An intensive electromagnetic field analysis was performed by applying rigorous coupled-wave analysis to a reasonably constructed micrometer-level periodic grating seam structure, and the phase jump appearing in millimeter-scale seams of large-aperture tiled gratings was obtained accurately.
A key objective for upcoming surveys, and when re-analysing archival data, is the identification of variable stellar sources. However, the selection of these sources is often complicated by the unavailability of light curve data. Utilising a self-organising map (SOM), we demonstrate the selection of diverse variable source types from a catalogue of variable and non-variable SDSS Stripe 82 sources whilst employing only the median $u-g$, $g-r$, $r-i$, and $i-z$ photometric colours for each source as input, without using source magnitudes. This includes the separation of main sequence variable stars that are otherwise degenerate with non-variable sources ($u-g$,$g-r$) and ($r-i$,$i-z$) colour-spaces. We separate variable sources on the main sequence from all other variable and non-variable sources with a purity of $80.0\%$ and completeness of $25.1\%$, figures which can be modified depending on the application. We also explore the varying ability of the same method to simultaneously select other types of variable sources from the heterogeneous sample, including variable quasars and RR-Lyrae stars. The demonstrated ability of this method to select variable main sequence stars in colour-space holds promise for application in future survey reduction pipelines and for the analysis of archival data, where light curves may not be available or may be prohibitively expensive to obtain.
Dispersion relations of electrostatic surface waves propagating in magnetized plasmas contained in an infinite duct and in an infinite cylindrical column surrounded by vacuum are derived by means of a Vlasov equation and fluid equations, respectively. The kinematic boundary condition imposed on the distribution function, the specular reflection conditions on the four sides of a duct, can be satisfied by placing infinite number of fictitious surface charge sheets spaced by the duct widths. The Vlasov equation that includes these surface charge sheets is solved by summing up the contribution due to the infinite charge sheets. The method of placing appropriate fictitious surface charge sheets enables one to treat the surface waves in bounded plasmas of Cartesian structure with mathematical efficiency, kinetically. The kinetic duct dispersion relation is compared with the dispersion relation for the magnetized cylindrical plasma column. When the square duct cross-sectional area as well as the cylinder radius become infinity, both dispersion relations become the dispersion relation of the upper-hybrid wave.
A high-energy pulsed vacuum ultraviolet (VUV) solid-state laser at 177 nm with high peak power by the sixth harmonic of a neodymium-doped yttrium aluminum garnet (Nd:YAG) amplifier in a KBe2BO3F2 prism-coupled device was demonstrated. The ultraviolet (UV) pump laser is a 352 ps pulsed, spatial top-hat super-Gaussian beam at 355 nm. A high energy of a 7.12 mJ VUV laser at 177 nm is obtained with a pulse width of 255 ps, indicating a peak power of 28 MW, and the conversion efficiency is 9.42% from 355 to 177 nm. The measured results fitted well with the theoretical prediction. It is the highest pulse energy and highest peak power ever reported in the VUV range for any solid-state lasers. The high-energy, high-peak-power, and high-spatial-uniformity VUV laser is of great interest for ultra-fine machining and particle-size measurements using UV in-line Fraunhofer holography diagnostics.
We examine the gravity-driven flow of a thin film of viscous fluid spreading over a rigid plate that is lubricated by another viscous fluid. We model the flow over such a ‘soft’ substrate by applying the principles of lubrication theory, assuming that vertical shear provides the dominant resistance to the flow. We do so in axisymmetric and two-dimensional geometries in settings in which the flow is self-similar. Different flow regimes arise, depending on the values of four key dimensionless parameters. As the viscosity ratio varies, the behaviour of the intruding layer ranges from that of a thin coating film, which exerts negligible traction on the underlying layer, to a very viscous gravity current spreading over a low-viscosity, near-rigid layer. As the density difference between the two layers approaches zero, the nose of the intruding layer steepens, approaching a shock front in the equal-density limit. We characterise a frontal stress singularity, which forms near the nose of the intruding layer, by performing an asymptotic analysis in a small neighbourhood of the front. We find from our asymptotic analysis that unlike single-layer viscous gravity currents, which exhibit a cube-root frontal singularity, the nose of a viscous gravity current propagating over another viscous fluid instead exhibits a square-root singularity, to leading order. We also find that large differences in the densities between the two fluids give rise to flows similar to that of thin films of a single viscous fluid spreading over a rigid, yet mobile, substrate.
We investigate the turbulence below a quasi-flat free surface, focusing on the energy transport in space and across scales. We leverage a large zero-mean-flow tank where homogeneous turbulence is generated by randomly actuated jets. A wide range of Reynolds number is spanned, reaching sufficient scale separation for the emergence of an inertial sub-range. Unlike previous studies, the forcing extends through the source layer, although the surface deformation remains millimetric. Particle image velocimetry along a surface-normal plane resolves from the dissipative to the integral scales. The contributions to turbulent kinetic energy from both vertical and horizontal components of velocity approach the prediction based on rapid distortion theory as the Reynolds number is increased, indicating that discrepancies among previous studies are likely due to differences in the forcing. At odds with the theory, however, the integral scale of the horizontal fluctuations grows as the surface is approached. This is rooted in the profound influence exerted by the surface on the inter-scale energy transfer: along horizontal separations, the direct cascade of energy in horizontal fluctuations is hindered, while an inverse cascade of that in vertical fluctuations is established. This is connected to the structure of upwellings and downwellings. The former, characterized by somewhat larger spatial extent and stronger intensity, are associated with extensional surface-parallel motions. They thus transfer energy to the larger horizontal scales, prevailing over downwellings which favour the compression (and concurrent vertical stretching) of the eddies. Both types of structures extend to depths between the integral scale and the Taylor microscale.
Stall cells are transverse cellular patterns that often appear on the suction side of airfoils near stalling conditions. Wind-tunnel experiments on a NACA4412 airfoil at Reynolds number ${Re}=3.5 \times 10^5$ show that they appear for angles of attack larger than $\alpha = 11.5^{\circ }\ (\pm 0.5^{\circ })$. Their onset is further investigated based on global stability analyses of turbulent mean flows computed with the Reynolds-averaged Navier–Stokes (RANS) equations. Using the classical Spalart–Allmaras turbulence model and following Plante et al. (J. Fluid Mech., vol. 908, 2021, A16), we first show that a three-dimensional stationary mode becomes unstable for a critical angle of attack $\alpha = 15.5^{\circ }$ which is much larger than in the experiments. A data-consistent RANS model is then proposed to reinvestigate the onset of these stall cells. Through an adjoint-based data-assimilation approach, several corrections in the turbulence model equation are identified to minimize the differences between assimilated and reference mean-velocity fields, the latter reference field being extracted from direct numerical simulations. Linear stability analysis around the assimilated mean flow obtained with the best correction is performed first using a perturbed eddy-viscosity approach which requires the linearization of both RANS and turbulence model equations. The three-dimensional stationary mode becomes unstable for angle $\alpha = 11^{\circ }$ which is in significantly better agreement with the experimental results. The interest of this perturbed eddy-viscosity approach is demonstrated by comparing with results of two frozen eddy-viscosity approaches that neglect the perturbation of the eddy viscosity. Both approaches predict the primary destabilization of a higher-wavenumber mode which is not experimentally observed. Uncertainties in the stability results are quantified through a sensitivity analysis of the stall cell mode's eigenvalue with respect to residual mean-flow velocity errors. The impact of the correction field on the results of stability analysis is finally assessed.
Fiber Bragg grating-based Raman oscillators are capable of achieving targeted frequency conversion and brightness enhancement through the provision of gain via stimulated Raman scattering across a broad gain spectrum. This capability renders them an exemplary solution for the acquisition of high-brightness, specialized-wavelength lasers. Nonetheless, the output power of all-fiber Raman oscillators is typically limited to several hundred watts, primarily due to limitations in injectable pump power and the influence of higher-order Raman effects, which is inadequate for certain application demands. In this study, we introduce an innovative approach by employing a graded-index fiber with a core diameter of up to 150 μm as the Raman gain medium. This strategy not only enhances the injectable pump power but also mitigates higher-order Raman effects. Consequently, we have successfully attained an output power of 1780 W for the all-fiber Raman laser at 1130 nm, representing the highest output power in Raman fiber oscillators with any configuration reported to date.
We report a novel pilot project to characterise intra-night optical variability (INOV) of an extremely rare type of quasar, which has recently been caught in the act of transiting from a radio-quiet to radio-loud state, on a decadal time scale. Such rare transitions may signify a recurrence, or conceivably the first switch-on of jet activity in optically luminous quasars. The newly formed jet could well be jittery and unsteady, both in power and direction. The optically brightest among such radio-state transition candidates, the quasar J0950+5128 ($z = 0.2142$), was monitored by us with dense sampling in the R-band, during 2020-21 in 6 sessions, each lasting $ \gt $ 4 hours. This is the first attempt to characterise the INOV properties associated with this recently discovered, extremely rarely observed phenomenon of quasar radio-state transition. The non-detection of INOV in any of the 6 sessions, down to the 1-2% level, amounts to a lack of evidence for a blazar-like optical activity, $\sim$ 2 years after its transition to radio-loud state was found. The only INOV feature detected in J0950+5128 during our observational campaign was a $\sim$ 0.15-mag spike lasting < 6 minutes, seen at 13.97 UT on 18-March-2021. We also report the available optical light curves of this quasar from the Zwicky Transient Facility survey, which indicate that it had experienced a phase of INOV activity around the time its transition to the radio-loud state was detected, however that phase did not sustain until the launch of our INOV campaign $\sim$ 2 years later.
This experimental study employs Bayesian optimisation to maximise the cross-flow (transverse) flow-induced vibration (FIV) of an elastically mounted thin elliptical cylinder by implementing axial (or angular) flapping motions. The flapping amplitude was in proportion to the vibration amplitude, with a relative phase angle imposed between the angular and transverse displacements of the cylinder. The control parameter space spanned over the ranges of proportional gain and phase difference of $0 \leq K_p^* \leq 5$ and $0 \leq \phi _d \leq 360^\circ$, respectively, over a reduced velocity range of $3.0 \leqslant {U^*} = U/({{f_{nw}}} b) \leqslant 8.5$. The corresponding Reynolds number range was $1250 \leqslant {{Re}} =(U b)/\nu \leqslant 3580$. Here, $U$ is the free stream velocity, $b$ is the major cross-sectional diameter of the cylinder, ${{f_{nw}}}$ is the natural frequency of the system in quiescent fluid (water) and $\nu$ is the kinematic viscosity of the fluid. It was found that the controlled body rotation extended the wake-body synchronisation across the entire ${U^*}$ range tested, with a larger amplitude response than the non-rotating case for all flow speeds. Interestingly, two new wake-body synchronisation regimes were identified, which have not been reported in previous studies. As this geometry acts as a ‘hard-oscillator’ for ${U^*} \geqslant 6.3$, an adaptive gain (i.e. one that varies as a function of oscillation amplitude) was also implemented, allowing the body vibration, achieved for a non-rotating cylinder using increasing ${U^*}$ increments, to be excited from rest. The findings of the present study hold potential implications for the use of FIV as a means to efficiently extract energy from free-flowing water sources, a topic of increasing interest over the last decade.
Continuum kinetic simulations are increasingly capable of resolving high-dimensional phase space with advances in computing. These capabilities can be more fully explored by using linear kinetic theory to initialize the self-consistent field and phase space perturbations of kinetic instabilities. The phase space perturbation of a kinetic eigenfunction in unmagnetized plasma has a simple analytic form, and in magnetized plasma may be well approximated by truncation of a cyclotron-harmonic expansion. We catalogue the most common use cases with a historical discussion of kinetic eigenfunctions and by conducting nonlinear Vlasov–Poisson and Vlasov–Maxwell simulations of singlemode and multimode two-stream, loss-cone and Weibel instabilities in unmagnetized and magnetized plasmas with one- and two-dimensional geometries. Applications to quasilinear kinetic theory are discussed and applied to the bump-on-tail instability. In order to compute eigenvalues we present novel representations of the dielectric function for ring distributions in magnetized plasmas with power series, hypergeometric and trigonometric integral forms. Eigenfunction phase space fluctuations are visualized for prototypical cases such as the Bernstein modes to build intuition. In addition, phase portraits are presented for the magnetic well associated with nonlinear saturation of the Weibel instability, distinguishing current-density-generating trapping structures from charge-density-generating ones.
We experimentally investigate the effect of Reynolds number ($Re$) on the turbulence induced by the motion of bubbles in a quiescent Newtonian fluid at small $Re$. The energy spectra, $E(k)$, are determined from the decaying turbulence behind the bubble swarm obtained using particle image velocimetry. We show that when $Re \sim O(100)$, the slope of the normalized energy spectra is no longer independent of the gas volume fraction and the $k^{-3}$ subrange is significantly narrower, where $k$ is the wavenumber. This is further corroborated using second-order longitudinal velocity structure function and spatial correlation of the velocity behind the bubble swarm. On further decreasing the bubble Reynolds number ($O(1) < Re < O(10)$), the signature $k^{-3}$ of the energy spectra for the bubble-induced turbulence is replaced by $k^{-5/3}$ scaling. Thus, we provide experimental evidence to the claim by Mazzitelli et al. (Phys. Fluids, vol. 15, 2003, pp. L5–L8) that at low Reynolds numbers the normalized energy spectra of the bubble-induced turbulence will no longer show the $k^{-3}$ scaling because of the absence of bubble wake and that the energy spectra will depend on the number of bubbles, thus being non-universal.
This study conducts experimental investigations into wake-induced vibration (WIV) of a circular cylinder placed downstream of an oscillating cylinder. Surprisingly, it is observed that the previously identified WIV phenomenon, characterized by a sustained increase in amplitude at higher reduced velocities, does not occur when the upstream cylinder oscillates at large amplitudes. Instead, a different phenomenon, which we refer to as the ‘wake-captured vibration’, becomes dominant. The experiments reveal a negative correlation between the vortex-induced vibration amplitude response of the upstream cylinder and the WIV amplitude response of the downstream cylinder. Through a quasi-steady and linear instability analysis, the study demonstrates that the previously proposed wake-displacement mechanism may not be applicable for predicting the cylinder WIV response in the wake of an oscillating body. This is because the lift force gradients across the wake, measured through stationary cylinder experiments, decrease significantly when the upstream cylinder vibrates at higher amplitudes. Consequently, actively controlled vibration experiments are conducted to systematically map the hydrodynamic properties of the downstream cylinder vibrating in the wake of an oscillating cylinder. The findings align with observations from free-vibration experiments, and help to explain the amplitude and frequency response of WIV. Additionally, wake visualization through particle image velocimetry is conducted to provide further insights into the complex wake and vortex–body interactions.
We study the two-dimensional creeping flow of a viscoelastic fluid around a cylinder confined between two plates parallel to its axis. First, we solve the governing equations under steady state with our novel stabilized finite-element formulation to obtain converged solutions even at very high Weissenberg numbers. Then, we examine the stability of this solution by perturbing all flow variables and solving the corresponding eigenvalue problem. At critical conditions, a stable asymmetric flow arises, in which more fluid passes from either the upper or the lower gap between the cylinder and the channel wall. Both shear-thinning and elasticity play a crucial role on the onset and subsequent evolution of the instability. Energy analysis shows that the terms of the constitutive equation corresponding to apparent strain-rate thinning and material extensibility are responsible for the flow destabilization. The instability is present at a wider range of flow conditions when the material is more elastic and when the solvent contribution is smaller. The instability is also promoted by increasing the confinement. Beyond the critical conditions, asymmetric flow profiles vanish when the flow is so intense that thinning effects are not important anymore. The critical Weissenberg number for instability inception and cessation depends on material properties and geometry exponentially and linearly, respectively. Furthermore, the instability arises even in a seemingly non-shear-thinning fluid, i.e. one with constant shear viscosity in simple shear, when the solvent contribution is minimal, because of the apparent thinning effect that is created by the convection of the viscoelastic stresses. Finally, models with extension-rate thinning trigger the instability at limited flow conditions, when the shear viscosity decreases with the shear rate, and the normal stresses at the wake of the cylinder are still important. These results agree with previous experiments and simulations, and give new insights on the physical mechanism that triggers this flow instability.
In the astrophysics community it is common practice to model collisionless dust, entrained in a gas flow, as a pressureless fluid. However, a pressureless fluid is fundamentally different from a collisionless fluid – the latter of which generically possess a non-zero anisotropic pressure or stress tensor. In this paper we derive a fluid model for collisionless dust, entrained in a turbulent gas, starting from the equations describing the motion of individual dust grains. We adopt a covariant formulation of our model to allow for the geometry and coordinate systems prevalent in astrophysics, and provide a closure valid for the accretion disc context. We show that the continuum mechanics properties of a dust fluid corresponds to a higher-dimensional anisotropic Maxwell fluid, after the extra dimensions are averaged out, with a dynamically important rheological stress tensor. This higher-dimensional treatment has the advantage of keeping the dust velocity and velocity of the fluid seen, and their respective moments, on the same footing. This results in a simplification of the constitutive relation describing the evolution of the dust rheological stress.
The Earth magnetopause, when sufficiently plane and stationary at a local scale, can be considered as a ‘quasi-tangential’ discontinuity, since the normal component of the magnetic field $B_n$ is typically very small but not zero. Contrary to observations, the ‘classic theory of discontinuities’ predicts that rotational and compressional jumps should be mutually exclusive in the general case $B_n \ne 0$, but allows only one exception: the tangential discontinuity provided that $B_n$ is strictly zero. Here we show that finite Larmor radius (FLR) effects play an important role in the quasi-tangential case, whenever the ion Larmor radius is not fully negligible with respect to the magnetopause thickness. By including FLR effects, the results suggest that a rotational discontinuity undergoes a change comparable to the change of a shear Alfvén into a kinetic Alfvén wave when considering linear modes. For this new kind of discontinuity, the co-existence of rotational and compressional variations at the magnetopause does no more imply that this boundary is a strict tangential discontinuity, even in one-dimensional (1-D)-like regions far from X lines if any. This result may lead to important consequences concerning the oldest and most basic questions of magnetospheric physics: how can the magnetopause be open, where and when? While the role of FLR is established theoretically, in this paper we show that it can be proved experimentally. For this, we make use of magnetospheric multiscale mission (MMS) data and process them with the most recent available four spacecraft tools. First, we present the different processing techniques that we use to estimate spatial derivatives, such as $grad(B)$ and $div(P)$, and the magnetopause normal direction. We point out why this normal direction must be determined with extremely high accuracy to make the conclusions unambiguous. Then, the results obtained by these techniques are presented in a detailed case study and on a statistical basis.