Stauffer (2021) shows that when Americans perceive their legislatures as having more gender diversity, they perceive these institutions to be more responsive. In this letter, we use an independently fielded survey to validate Stauffer’s findings and investigate whether her findings extend to race. We successfully replicate Stauffer’s analyses. We also newly demonstrate that perceptions of Black representation in legislatures are related to increased external efficacy, and perceptions of gender descriptive representation are associated with increased trust. Such findings underscore the importance of studying citizens’ perceptions of descriptive representation to understand their attitudes toward government.

Digital twins are a new paradigm for our time, offering the possibility of interconnected virtual representations of the real world. The concept is very versatile and has been adopted by multiple communities of practice, policymakers, researchers, and innovators. A significant part of the digital twin paradigm is about interconnecting digital objects, many of which have previously not been combined. As a result, members of the newly forming digital twin community are often talking at cross-purposes, based on different starting points, assumptions, and cultural practices. These differences are due to the philosophical world-view adopted within specific communities. In this paper, we explore the philosophical context which underpins the digital twin concept. We offer the building blocks for a philosophical framework for digital twins, consisting of 21 principles that are intended to help facilitate their further development. Specifically, we argue that the philosophy of digital twins is fundamentally holistic and emergentist. We further argue that in order to enable emergent behaviors, digital twins should be designed to reconstruct the behavior of a physical twin by “dynamically assembling” multiple digital “components”. We also argue that digital twins naturally include aspects relating to the philosophy of artificial intelligence, including learning and exploitation of knowledge. We discuss the following four questions (i) What is the distinction between a model and a digital twin? (ii) What previously unseen results can we expect from a digital twin? (iii) How can emergent behaviours be predicted? (iv) How can we assess the existence and uniqueness of digital twin outputs?

Multi-layer networks arise when more than one type of relation is observed on a common set of actors. Modeling such networks within the exponential-family random graph (ERG) framework has been previously limited to special cases and, in particular, to dependence arising from just two layers. Extensions to ERGMs are introduced to address these limitations: Conway–Maxwell–Binomial distribution to model the marginal dependence among multiple layers; a “layer logic” language to translate familiar ERGM effects to substantively meaningful interactions of observed layers; and nondegenerate triadic and degree effects. The developments are demonstrated on two previously published datasets.

Negative human–wildlife interactions are a growing problem, particularly for people living near protected areas and wildlife refuges. In Kenya, African savannah elephants Loxodonta africana threaten food security for subsistence farmers by crop foraging, which can jeopardize conservation efforts if farmers retaliate against elephants. To inform conservation and management, this study had three objectives: (1) to evaluate stakeholder participatory models of human–elephant conflict; (2) to note any novel or underrepresented variables in the models; and (3) to determine if there were indicators for assessing the success of mitigation programmes using a biocultural approach. We conducted participatory modelling sessions in six villages in rural Kenya using fuzzy cognitive mapping (n = 206 participants). Farmers created group visual models with variables related to conflict with elephants. A total of 14 variables were common across all six villages, with the two highest centrality scores (a measure of importance to overall dynamics) associated with income and feelings of security. Most variables fell into two categories: environmental interactions, and policy and management. Multiple variables such as road infrastructure (drivers) and soil compaction (consequences) were identified as aspects of conflicts that are under-reported or absent in scientific literature, as well as potential socio-cultural indicators. The participatory method used is a tool for gaining more refined insights into interactions with elephants, with implications for other complex conservation issues or wildlife interactions. A more holistic view of the impacts of human–elephant interactions as demonstrated here can lead to sustainable, co-developed programmes that benefit both farmer livelihoods and elephant conservation.

We provide scaling relations for the Nusselt number $Nu$ and the friction coefficient $C_{S}$ in sheared Rayleigh–Bénard convection, i.e. in Rayleigh–Bénard flow with Couette- or Poiseuille-type shear forcing, by extending the Grossmann & Lohse (J. Fluid Mech., vol. 407, 2000, pp. 27–56, Phys. Rev. Lett., vol. 86, 2001, pp. 3316–3319, Phys. Rev. E, vol. 66, 2002, 016305, Phys. Fluids, vol. 16, 2004, pp. 4462–4472) theory to sheared thermal convection. The control parameters for these systems are the Rayleigh number $Ra$, the Prandtl number $Pr$ and the Reynolds number $Re_S$ that characterises the strength of the imposed shear. By direct numerical simulations and theoretical considerations, we show that, in turbulent Rayleigh–Bénard convection, the friction coefficients associated with the applied shear and the shear generated by the large-scale convection rolls are both well described by Prandtl's (Ergeb. Aerodyn. Vers. Gött., vol. 4, 1932, pp. 18–29) logarithmic friction law, suggesting some kind of universality between purely shear-driven flows and thermal convection. These scaling relations hold well for $10^6 \leq Ra \leq 10^8$, $0.5 \leq Pr \leq 5.0$, and $0 \leq Re_S \leq 10^4$.

Knowledge of snow and firn-density change is needed to use elevation-change measurements to estimate glacier mass change. Additionally, firn-density evolution on glaciers is closely connected to meltwater percolation, refreezing and runoff, which are key processes for glacier mass balance and hydrology. Since 2016, the U.S. Geological Survey Benchmark Glacier Project has recovered firn cores from a site on Wolverine Glacier in Alaska's Kenai Mountains. We use annual horizons in repeat cores to track firn densification and meltwater retention over seasonal and interannual timescales, and we use density measurements to quantify how the firn air content (FAC) changes through time. The results suggest the firn is densifying due primarily to compaction rather than refreezing. Liquid-water retention in the firn is transient, likely due to gravity-fed drainage and irreducible-water-content decreases that accompany decreasing porosity. We show that the uncertainty (±60 kg m−3) in the commonly used volume-to-mass conversion factor of 850 kg m−3 is an underestimation when glacier-wide FAC variability exceeds 12% of the glacier-averaged height change. Our results demonstrate how direct measurements of firn properties on mountain glaciers can be used to better quantify the uncertainty in geodetic volume-to-mass conversions.

Background: Nursing home (NH) residents are at high risk of COVID-19 from exposure to infected staff and other residents. Understanding SARS-CoV-2 viral RNA kinetics in residents and staff can guide testing, isolation, and return to work recommendations. We sought to determine the duration of antigen test and polymerase chain reaction (PCR) positivity in a cohort of NH residents and staff. Methods: We prospectively collected data on SARS-CoV-2 viral kinetics from April 2023 through November 2023. Staff and residents could enroll prospectively or upon a positive test (identified through routine clinical testing, screening, or outbreak response testing). Participating facilities performed routine clinical testing; asymptomatic testing of contacts was performed within 48 hours if an outbreak or known exposure occurred and upon (re-) admission. Enrolled participants who tested positive for SARS-CoV-2 were re-tested daily for 14 days with both nasal antigen and nasal PCR tests. All PCR tests were run by a central lab with the same assay. We conducted a Kaplan-Meier survival analysis on time to first negative test restricted to participants who initially tested positive (day zero) and had at least one test ≥10 days after initially testing positive with the same test type; a participant could contribute to both antigen and PCR survival curves. We compared survival curves for staff and residents using the log-rank test. Results: Twenty-four nursing homes in eight states participated; 587 participants (275 residents, 312 staff) enrolled in the evaluation, participants were only tested through routine clinical or outbreak response testing. Seventy-two participants tested positive for antigen; of these, 63 tested PCR-positive. Residents were antigen- and PCR-positive longer than staff (Figure 1), but this finding is only statistically significant (p=0.006) for duration of PCR positivity. Five days after the first positive test, 56% of 50 residents and 59% of 22 staff remained antigen-positive; 91% of 44 residents and 79% of 19 staff were PCR-positive. Ten days after the first positive test, 22% of 50 residents and 5% of 22 staff remained antigen-positive; 61% of 44 residents and 21% of 19 staff remained PCR-positive. Conclusions: Most NH residents and staff with SARS-CoV-2 remained antigen- or PCR-positive 5 days after the initial positive test; however, differences between staff and resident test positivity were noted at 10 days. These data can inform recommendations for testing, duration of NH resident isolation, and return to work guidance for staff. Additional viral culture data may strengthen these conclusions.

Disclosure: Stefan Gravenstein: Received consulting and speaker fees from most vaccine manufacturers (Sanofi, Seqirus, Moderna, Merck, Janssen, Pfizer, Novavax, GSK, and have or expect to receive grant funding from several (Sanofi, Seqirus, Moderna, Pfizer, GSK). Lona Mody: NIH, VA, CDC, Kahn Foundation; Honoraria: UpToDate; Contracted Research: Nano-Vibronix

We present radio observations of the galaxy cluster Abell S1136 at 888 MHz, using the Australian Square Kilometre Array Pathfinder radio telescope, as part of the Evolutionary Map of the Universe Early Science program. We compare these findings with data from the Murchison Widefield Array, XMM-Newton, the Wide-field Infrared Survey Explorer, the Digitised Sky Survey, and the Australia Telescope Compact Array. Our analysis shows the X-ray and radio emission in Abell S1136 are closely aligned and centered on the Brightest Cluster Galaxy, while the X-ray temperature profile shows a relaxed cluster with no evidence of a cool core. We find that the diffuse radio emission in the centre of the cluster shows more structure than seen in previous low-resolution observations of this source, which appeared formerly as an amorphous radio blob, similar in appearance to a radio halo; our observations show the diffuse emission in the Abell S1136 galaxy cluster contains three narrow filamentary structures visible at 888 MHz, between $\sim$80 and 140 kpc in length; however, the properties of the diffuse emission do not fully match that of a radio (mini-)halo or (fossil) tailed radio source.

OBJECTIVES/GOALS: In this study, we aim to report the role of porins and blaCTX-M β-lactamases among Escherichia coli and Klebsiella pneumoniae, focusing on emerging carbapenem resistant Enterobacterales (CRE) subtypes, including non-carbapenemase producing Enterobacterales (NCPE) and ertapenem-resistant but meropenem-susceptible (ErMs) strains. METHODS/STUDY POPULATION: Whole genome sequencing was conducted on 76 carbapenem-resistant isolates across 5 hospitals in San Antonio, U.S. Among these, NCP isolates accounted for the majority of CRE (41/76). Identification and antimicrobial susceptibility testing (AST) results were collected from the clinical charts. Repeat speciation was determined through whole genome sequencing (WGS) analysis and repeat AST, performed with microdilution or ETEST®. Minimum inhibitory concentrations (MIC) were consistent with Clinical and Laboratory Standards Institute (CLSI M100, ED33). WGS and qPCR were used to characterize the resistome of all clinical CRE subtypes, while western blotting and liquid chromatography with tandem mass spectrometry (LC-MS-MS) were used to determine porin expression and carbapenem hydrolysis, respectively. RESULTS/ANTICIPATED RESULTS: blaCTX-Mwas found to be most prevalent among NCP isolates (p = 0.02). LC-MS/MS analysis of carbapenem hydrolysis revealed that blaCTX-M-mediated carbapenem hydrolysis, indicating the need to reappraise the term, “non-carbapenemase (NCP)®” for quantitatively uncharacterized CRE strains harboring blaCTX-M. Susceptibility results showed that 56% of all NCPE isolates had an ErMs phenotype (NCPE vs. CPE, p < 0.001), with E. coli driving the phenotype (E. coli vs. K. pneumoniae, p < 0.001). ErMs strains carrying blaCTX-M, had 4-fold more copies of blaCTX-M than ceftriaxone-resistant but ertapenem-susceptible isolates (3.7 v. 0.9, p < 0.001). Immunoblot analysis demonstrated the absence of OmpC expression in NCP-ErMs E. coli, with 92% of strains lacking full contig coverage ofompC. DISCUSSION/SIGNIFICANCE: Overall, this work provides evidence of a collaborative effort between blaCTX-M and OmpC in NCP strains that confer resistance to ertapenem but not meropenem. Clinically, CRE subtypes are not readily appreciated, potentially leading to mismanagement of CRE infected patients. A greater focus on optimal treatments for CRE subtypes is needed.

Natural zeolites may represent one of the greatest under-utilized resources of the mineral world. Even with their unique character and properties, issues of consistency, homogeneity and purity preclude them from many premium applications. We report a simple method to upgrade mineral sedimentary Na-chabazite from the well known Bowie, Arizona, deposit to near synthetic purity and consistency. During this alkaline-silicate digestion process, initially soft chabazite ore granules gain substantial mechanical strength. This may allow direct employment in adsorption and purification processes without the need for binding and forming. These granules manifest significantly improved adsorption properties, including enhanced water and CO2 adsorptivity.

Mineral chabazite has shown the unusual ability to surface template nanometal particles, especially Ag. A chabazite analog was synthesized from delaminated metakaolin. The chabazite formed retained the platy morphology of the base clay. This morphology is ideal for displaying surface-supported nanometal particles. The synthetic chabazite analog demonstrated the ability to form and support large concentrations of Ag nanoparticles, as observed in the related natural mineral. Due to greater Al content, the synthetic chabazite manifests significantly improved capacity for the formation of such Ag nanoparticles. As in the case of the mineral chabazite, surface Ag nanoparticles of high uniformity were observed in the range of 5–6 nm.

The north coast of Peru is among the most extensively surveyed regions in the world, yet variation in research questions, sampling strategies and chronological and geospatial controls among survey projects makes comparison of disparate datasets difficult. To contextualise these issues, the authors present a systematic survey of satellite imagery focusing on hilltop fortifications in the Jequetepeque and Santa Valleys. This digital recontextualisation of pedestrian survey data demonstrates the potential of hybrid methodologies to substantially expand both the identification of archaeological sites within difficult terrain and, consequently, our understanding of the function of defensive sites.