Climate change has been shown to affect different aspects of society, with agriculture and the food system taking the highest hit. Several initiatives have been put in place to dampen such effects. Climate education could play an important role in the fight against climate change. Climate education ensures that farmers understand the anthropogenic causes of climate change and the principles underlying adaptation measures, hence informing adoption of sound adaptation measures. Although such theoretical underpinnings are clear, empirical evidence is lacking. We employ a multivariate probit model to empirically investigate the role of climate education in adoption of climate adaptation practices using data from Cameroon, whose humid tropical agroecology and forests are crucial to climate change mitigation in the Congo basin. Employing a linear model, we similarly evaluate the role of climate education on farm incomes as well as the role of perception of climate change. Our results show that climate education influences adoption of adaptation measures, especially simple and cost-effective measures. However, climate education does not affect farm income, neither does farmers’ perception of climate change. These results suggest that indigenous farmers may be more willing to choose a simple low-cost adaptation measure. The generated results are crucial for influencing climate change policy related to awareness building, education, and training for optimal adaptation efforts.

For near-future missions planed for Mars Sample Return (MSR), an international working group organized by the Committee on Space Research (COSPAR) developed the sample safety assessment framework (SSAF). For the SSAF, analytical instruments were selected by taking the practical limitations of hosting them within a facility with the highest level of biosafety precautions (biosafety level 4) and the precious nature of returned samples into account. To prepare for MSR, analytical instruments of high sensitivity need to be tested on effective Mars analogue materials. As an analogue material, we selected a rock core of basalt, a prominent rock type on the Martian surface. Two basalt samples with aqueous alteration cached in Jezero crater by the Perseverance rover are planned to be returned to Earth. Our previously published analytical procedures using destructive but spatially sensitive instruments such as nanoscale secondary ion mass spectrometry (NanoSIMS) and transmission electron microscopy coupled to energy-dispersive spectroscopy revealed microbial colonization at clay-filled fractures. With an aim to test the capability of an analytical instrument listed in SSAF, we now extend that work to conventional Fourier transform infrared (FT-IR) microscopy with a spatial resolution of 10 μm. Although Fe-rich smectite called nontronite was identified after crushing some portion of the rock core sample into powder, the application of conventional FT-IR microscopy is limited to a sample thickness of <30 μm. In order to obtain IR-based spectra without destructive preparation, a new technique called optical-photothermal infrared (O-PTIR) spectroscopy with a spatial resolution of 0.5 μm was applied to a 100 μm thick section of the rock core. By O-PTIR spectroscopic analysis of the clay-filled fracture, we obtained in-situ spectra diagnostic to microbial cells, consistent with our previously published data obtained by NanoSIMS. In addition, nontronite identification was also possible by O-PTIR spectroscopic analysis. From these results, O-PTIR spectroscopy is suggested be superior to deep ultraviolet fluorescence microscopy/μ-Raman spectroscopy, particularly for smectite identification. A simultaneous acquisition of the spatial distribution of structural motifs associated with biomolecules and smectites is critical for distinguishing biological material in samples as well as characterizing an abiotic background.

Making woody mulch (WM) from organic waste is one solution for repurposing waste. Our work had two primary objectives. First, we wanted to destermine the current use of WM as a soil cover, barriers to use, benefits, and possible motivations for adopting the use of WM by home and commercial growers for cultivating crops in Barbados and the Baltimore-Washington, DC metropolitan region in the USA. To accomplish this objective, we administered a survey to growers in both regions. Second, we wanted to determine the benefits of using WM in agricultural production for sweet potatoes (both regions) and Hungarian hot wax peppers (USA). We measured whether WM influenced crop survival, crop yield, crop nutrients, weed mass, and soil characteristics in replicated plots covered with a layer of WM or left bare. Growers reported that expense, availability, and ease of application were barriers to using WM. Despite the barriers, many growers were using, or had previously used, WM and reported myriad benefits, including improving plant yield and/or nutrients, preventing weed growth, maintaining soil moisture and reducing irrigation needs, improving soil fertility, reducing soil erosion, reducing compaction from heavy rain, and maintaining soil temperature. Our data from replicated field trials verified some of the potential benefits reported by growers. WM in some cases promoted higher crop survival and yield of sweet potatoes, suppressed weeds, conserved soil moisture, and maintained higher soil temperature. Understanding which crops benefit from WM and the longer-term effects of WM on crops and soil are deserving of future study.

Background: Despite significant advances in the treatment of neurological disorders, many conditions remain palliative. Neurologists are in a unique position as they are integral in providing patient centered care, understanding neurologic disease and illness trajectory, and how disease can affect patients’ sense of self and values. Currently, little is known about neurologists’ perceptions and challenges in care planning and palliative care for their patients. Methods: A qualitative approach was utilized with semi-structured interviews of ten neurologists. Data was analyzed using a constant comparative method (constructivist grounded theory). Results: Participants represented a broad spectrum of neurologist experience and subspecialties. Four theories were identified: (1) care planning and palliative care are high priorities, (2) neurologic diseases uniquely affect patients and require a dynamic, patient-centered care plan, (3) a care gap exists in providing palliative care for neurologic patients with multifactorial barriers, and (4) opportunities to improve care exist with continuing education, collaboration, and health system support. Conclusions: Neurologists have a key role in care planning and palliative care for patients with chronic neurological diseases. Our findings show that there is a gap in the provision of palliative care. Future directions may include exploring educational opportunities and dedicated health systems to improve care management.

OBJECTIVES/GOALS: The goal of this proposal is to develop a technology that combines calcium imaging via confocal microscopy, and force measurement via monolayer stress microscopy to perform simultaneous quantitative measurements of agonist-induced Ca2+ and mechanical signals in HASMCs. METHODS/STUDY POPULATION: The methods by which second messenger signals and changes in mechanical forces determine specific physiological responses are complex. Recent studies point to the importance of temporal and spatial encoding in determining signal specificity. Hence, approaches that probe both chemical and mechanical signals are needed. We combine hyperspectral imaging for second messenger signal measurements, monolayer stress microscopy for mechanical force measurements, and S8 analysis software for quantifying localized signals. Imaging was performed using an excitation-scanning hyperspectral microscope. Hyperspectral images were unmixed to identify signals from fluorescent labels and microparticles. Images were analyzed to quantify localized force dynamics through monolayer stress microscopy. RESULTS/ANTICIPATED RESULTS: Results indicate that localized and transient cellular signals can be quantified and mapped within cell populations. Importantly, these results establish a method for simultaneous interrogation of cellular signals and mechanical forces that may play synergistic roles in regulating downstream cellular physiology in confluent monolayers. DISCUSSION/SIGNIFICANCE: We will measure the distribution of chemical and mechanical signals within cells, providing insight into the dynamics of cell signaling. Studies will have implication in the understanding of infections, drug delivery in which non-uniform distributions of drugs are a certainty, and in understanding coordinated responses in cellular systems.

Anthropological research has long theorized that emergent food-producing economies catalyzed high levels of inequality in human societies, as evident in the earliest use of jewelry made from gold, copper, and other precious minerals among early agricultural populations. Although the US Southwest appears to have been an exception, we report the discovery of two Basketmaker II period necklaces constructed of green iridescent scarab beetle femora, which suggests a homologous association between emergent agriculture and inequality. Drawing insight from ethnography, archaeology, entomology, and evolutionary ecology, we hypothesize that these and other jewelry items of Basketmaker II culture were visually prominent, honest signals of socioeconomic capital that emerged during a period of surplus food production and incipient wealth accumulation. It appears that Basketmaker II societies—like other emergent food-producing economies around the world—grappled with the opportunities and challenges that arise with surplus production, albeit in a distinct way that involved visually striking insect and feather adornments as status signals. Archaeologists may have previously overlooked this behavior due to Western biases that privilege precious metals and minerals as prestige objects and archaeological biases that tend to view insects as food or agents of site disturbance.

The Great Black-backed Gull Larus marinus is a generalist species that inhabits temperate and arctic coasts of the north Atlantic Ocean. In recent years, there has been growing concern about population declines at local and regional scales; however, there has been no attempt to robustly assess Great Black-backed Gull population trends across its global range. We obtained the most recent population counts across the species’ range and analysed population trends at a global, continental, and national scale over the most recent three-generation period (1985–2021) following IUCN Red List criteria. We found that, globally, the species has declined by 43%–48% over this period (1.2–1.3% per annum, respectively), from an estimated 291,000 breeding pairs to 152,000–165,000 breeding pairs under two different scenarios. North American populations declined more steeply than European ones (68% and 28%, respectively). We recommend that Great Black-backed Gull should be uplisted from ‘Least Concern’ to ‘Vulnerable’ on the IUCN Red List of Threatened Species under criterion A2 (an estimated reduction in population size >30% over three generations).

The Sheep Mountain juniper bark net, originally thought to be of Paleoindian age, was redated by Sundstrom and Walker (2021) to the Late Prehistoric period. Although the original investigators convincingly argued that the net was intended for use with mountain sheep or deer, Sundstrom and Walker suggest it was used to trap small game such as rabbits or sage grouse. Unfortunately, the authors ignore important information presented by the original investigators and misrepresent the archaeological record of the immediate area. The Sheep Mountain net is still best interpreted as designed for use to trap mountain sheep and deer.

This article examines the origins and dynamics of an extraordinary wave of protests in Hong Kong in 2019–2020. Despite lacking visible political opportunities and organizational resources, the protest movement drew resilient, mass participation unparalleled in the city's history and much of the world. Drawing from original on-site surveys and online datasets, we conceptualize the Anti-Extradition Law Amendment Bill Movement as a form of “total mobilization from below.” The totality of the mobilization depended on a set of interactive mechanisms: abeyant civil society networks concealed after the 2014 Umbrella Movement were activated by threats over extradition and institutional decay, whereas affective ties developed through conflicts and mutual assistance were amplified by digital communication. The movement's characteristics in terms of protest scale, mobilizing structure, use of alternative spaces, and group solidarity are examined. The spasmodic moments of mobilization are explained by a nexus of network building that took place in an unreceptive environment and at a critical juncture. The roles of threats and emotions in mass mobilizations are also analysed.

Since the Anglican Church in England and Wales began to build schools long before the state developed machinery to do so, around a quarter of all primary schools remain connected with the Anglican Church. The church school inspection system maintains that Anglican schools have a distinctive ethos. The Student Voice Project argues that school ethos is generated by the implicit collective values, beliefs and behaviours of the students, and was designed to give explicit voice to the students in response to six specific areas of school life identified by the Anglican school inspection criteria as relevant to school ethos. Drawing on data provide by 8,111 year-five and year-six students attending Church in Wales primary schools, the present study reports on the six ethos measures and on significant differences reported by female and male students, and by year-five and year-six students.