Direct numerical simulations of temporally developing compressible mixing layers have been performed to investigate the effects of large-scale structures (LSSs) on turbulent kinetic energy (TKE) budgets at convective Mach numbers ranging from $M_c=0.2$ to $1.8$ and at Taylor Reynolds numbers up to 290. In the core region of mixing layers, the volume fraction of low-speed LSSs decreases linearly with respect to the vertical distance at a Mach-number-independent rate. The contributions of low-speed LSSs to TKE, and its budget, including production, dissipation, pressure-strain and spatial diffusion terms, are primarily concentrated in the upper region of mixing layer. The streamwise and vertical mass flux coupling terms mainly transport TKE downwards in low-speed LSSs, and their magnitudes are comparable to the other dominant terms. Near the edges of LSSs, the sources and losses of all three components of TKE are completely different to each other, and dominated by turbulent diffusion, pressure diffusion, pressure-strain and dissipation terms. The TKE, their total variation and dissipation are significantly amplified at edges of low-speed LSSs, especially at the upper edge. This observation supports the existence of amplitude modulation exerted by the LSSs onto the near-edge small-scale structures in mixing layers. The level of amplitude modulation is strongest for the vertical velocity, followed by the streamwise velocity, and weakest for the spanwise velocity. Additionally, the amplitude modulation effect decreases significantly with increasing convective Mach number. The results on the amplitude modulation effect is helpful for developing predictive models of budget terms of TKE in mixing layers.

Active suspensions encompass a wide range of complex fluids containing microscale energy-injecting particles, such as cells, bacteria or artificially powered active colloids. Because they are intrinsically non-equilibrium, active suspensions can display a number of fascinating phenomena, including turbulent-like large-scale coherent motion and enhanced diffusion. Here, using a recently developed active fast Stokesian dynamics method, we present a detailed numerical study of the hydrodynamic diffusion in apolar active suspensions of squirmers. Specifically, we simulate suspensions of active but non-self-propelling spherical squirmers (or ‘shakers’), of either puller type or pusher type, at volume fractions from 0.5 % to 55 %. Our results show little difference between pulling and pushing shakers in their instantaneous and long-time dynamics, where the translational dynamics varies non-monotonically with the volume fraction, with a peak diffusivity at around 10 % to 20 %, in stark contrast to suspensions of self-propelling particles. On the other hand, the rotational dynamics tends to increase with the volume fraction as is the case for self-propelling particles. To explain these dynamics, we provide detailed scaling and statistical analyses based on the activity-induced hydrodynamic interactions and the observed microstructural correlations, which display a weak local order. Overall, these results elucidate and highlight the different effects of particle activity versus motility on the collective dynamics and transport phenomena in active fluids.

This study investigates experimentally the pressure fluctuations of liquids in a column under short-time acceleration. It demonstrates that the Strouhal number $St=L/(c\,\Delta t)$, where $L$, $c$ and $\Delta t$ are the liquid column length, speed of sound, and acceleration duration, respectively, provides a measure of the pressure fluctuations for intermediate $St$ values. On the one hand, the incompressible fluid theory implies that the magnitude of the averaged pressure fluctuation $\bar {P}$ becomes negligible for $St\ll 1$. On the other hand, the water hammer theory predicts that the pressure tends to $\rho cu_0$ (where $u_0$ is the change in the liquid velocity) for $St\geq O(1)$. For intermediate $St$ values, there is no consensus on the value of $\bar {P}$. In our experiments, $L$, $c$ and $\Delta t$ are varied so that $0.02 \leq St \leq 2.2$. The results suggest that the incompressible fluid theory holds only up to $St\sim 0.2$, and that $St$ governs the pressure fluctuations under different experimental conditions for higher $St$ values. The data relating to a hydrogel also tend to collapse to a unified trend. The inception of cavitation in the liquid starts at $St\sim 0.2$ for various $\Delta t$, indicating that the liquid pressure goes lower than the liquid vapour pressure. To understand this mechanism, we employ a one-dimensional wave propagation model with a pressure wavefront of finite thickness that scales with $\Delta t$. The model provides a reasonable description of the experimental results as a function of $St$.

Bioconvection is the prototypical active matter system for hydrodynamic instabilities and pattern formation in suspensions of biased swimming microorganisms, particularly at the dilute end of the concentration spectrum where direct cell–cell interactions are less relevant. Confinement is an inherent characteristic of such systems, including those that are naturally occurring or industrially exploited, so it is important to understand the impact of boundaries on the hydrodynamic instabilities. Despite recent interest in this area, we note that commonly adopted symmetry assumptions in the literature, such as for a vertical channel or pipe, are uncorroborated and potentially unjustified. Therefore, by employing a combination of analytical and numerical techniques, we investigate whether confinement itself can drive asymmetric plume formation in a suspension of bottom-heavy swimming microorganisms (gyrotactic cells). For a class of solutions in a vertical channel, we establish the existence of a first integral of motion, and reveal that asymptotic asymmetry is plausible. Furthermore, numerical simulations from both Lagrangian and Eulerian perspectives demonstrate with remarkable agreement that asymmetric solutions can indeed be more stable than symmetric; asymmetric solutions are, in fact, dominant for a large, practically important region of parameter space. In addition, we verify the presence of blip and varicose instabilities for an experimentally accessible parameter range. Finally, we extend our study to a vertical Hele-Shaw geometry to explore whether a simple linear drag approximation can be justified. We find that although two-dimensional bioconvective structures and associated bulk properties have some similarities with experimental observations, approximating near-wall physics in even the simplest confined systems remains challenging.

Vesicles are important surrogate structures made up of multiple phospholipids and cholesterol distributed in the form of a lipid bilayer. Tubular vesicles can undergo pearling – i.e. formation of beads on the liquid thread akin to the Rayleigh–Plateau instability. Previous studies have inspected the effects of surface tension on the pearling instabilities of single-component vesicles. In this study, we perform a linear stability analysis on a multicomponent cylindrical vesicle. We solve the Stokes equations along with the Cahn–Hilliard equation to develop the linearized dynamic equations governing the vesicle shape and surface concentration fields. This helps us to show that multicomponent vesicles can undergo pearling, buckling and wrinkling even in the absence of surface tension, which is a significantly different result from studies on single-component vesicles. This behaviour arises due to the competition between the free energies of phase separation, line tension and bending for this multi-phospholipid system. We determine the conditions under which axisymmetric and non-axisymmetric modes are dominant, and supplement our results with an energy analysis that shows the sources for these instabilities. Lastly, we delve into a weakly nonlinear analysis where we solve the nonlinear Cahn–Hilliard equation in the weak deformation limit to understand how mode-mixing alters the late time dynamics of coarsening. We show that in many situations, the trends from our simulations qualitatively match recent experiments (Yanagisawa et al., Phys. Rev. E, vol. 82, 2010, p. 051928).

During the excavation of Tol-e Sangi in southern Iran, tokens and a sealing were discovered in Pre-Pottery Neolithic (PPN, c. 7050–6900 BC) layers. As the oldest sealing found in Iran, this artefact suggests that storage and sealing practices were used during the PPN period in South-west Asia.