This work expands the knowledge on the diversity of Ordovician cephalopod assemblages of peri-Gondwana by describing 16 straight-shelled, annulated and/or reticulately ornamented specimens from the late Darriwilian and late Katian strata of the Prague Basin (Central Bohemia). Preservation facilitated the micro-CT investigation of internal shell structures in one of the specimens; in other specimens, the siphuncle and septa are not preserved, and these specimens are thus left in open nomenclature. In total, six species are recognized with one new species established: Anthoceras? sp. A, Anthoceras? sp. B, Sactorthoceras pustulatum new species, Dawsonoceras? sp. indet., Kionoceras? sp. indet., and Palaeodawsonocerina obscurum new combination. In addition, the holotype of Orthoceras evictum was examined and re-interpreted as a conulariid rather than a cephalopod. Paleogeographically, these taxa indicate mixed Baltican, Avalonian and peri-Gondwanan affinities for the late Darriwilian and late Katian cephalopod assemblages.

UUID: http://zoobank.org/fcf79203-e864-47ed-bf71-12f475fa55a9

Dicamba-resistant (DR) soybean cultivars are essential elements in managing broadleaf weeds in modern production systems. However, limited information is available regarding yield reductions associated with dicamba rates that were previously registered for postemergence weed control and off-label dicamba rates in these cultivars. This study aimed to characterize and quantify the effects of postemergence dicamba applications on two DR soybean cultivars. Field trials were conducted in 2022 and 2023, with dicamba applied at 0 to 1,440 g ae ha⁻¹ during the V5 to V6 stages. Visible injury increased with dicamba rate, reaching 18% (Cultivar A) to 20% (Cultivar B) at 1,440 g ae ha⁻¹ at 3 d after treatment, but symptoms declined to <10% by 4 wk after treatment (WAT). Chlorophyll fluorescence was not significantly affected at 2 and 4 WAT. Height reduction at 4 WAT occurred only at the highest dicamba rate (1,440 g ae ha⁻¹), but differences disappeared by maturity. Dry biomass reduction was also dose-dependent, reaching 16% for Cultivar A and 10% for Cultivar B at the highest rate. Pod reduction in DR soybean was minor (<3.5%) and not significant. Applications of dicamba from 288 to 864 g ae ha⁻¹ resulted in minimal yield reductions (<5%) and no significant biomass reduction. At a dicamba dose of 1,152 g ae ha⁻¹, yield reductions reached 7% and 9% for Cultivars A and B, respectively, while the highest rate (1,440 g ae ha⁻¹) resulted in yield reductions of 12% (Cultivar A) and 14% (Cultivar B). Despite over-the-top application restrictions, these results confirm that DR soybean cultivars tolerate rates (≤720 g ae ha⁻¹) of dicamba that were previously registered for postemergence weed control with minimal (<5%) yield reduction and recover rapidly from transient injury. However, applications above these rates can reduce yield by up to 14%, highlighting the importance of adhering to recommended dicamba use guidelines.

We aimed to determine the prevalence of antimicrobial resistance, carriage of Panton-Valentine leucocidin (PVL), and the clonal structure of MRSA isolates collected from skin and soft tissue infections at a tertiary care hospital in Pakistan. Between August 2021 and May 2022, 154 non-repetitive MRSA isolates were consecutively collected and characterized by antimicrobial susceptibility testing, SCCmec typing, spa typing, and detection of PVL by PCR. MLST clonal complexes (CCs) were inferred from spa type using the Based Upon Repeat Pattern (BURP) algorithm. High levels of resistance were observed to ciprofloxacin (85.7%), erythromycin (76.0%), sulfamethoxazole (68.8%), gentamicin (68.8%), fusidic acid (57.8%), tetracycline (55.8%), and clindamycin (42.2%). Clonal analysis revealed 16 lineages, with the most frequent being CC8-MRSA-IV (27.3%), PVL-positive “Bengal Bay” CC1/ST772-MRSA-V (26.0%), and CC1-MRSA-IV (16.2%). PVL was detected in 45.5% of isolates across multiple lineages. Our findings highlight the coexistence of high antimicrobial resistance and frequent PVL carriage among MRSA in Pakistan. Given the association of PVL with severe infections and the limited treatment options for multidrug-resistant strains, these data underscore a significant public health concern and the need for systematic surveillance and prudent antibiotic use.

The phylogenetic relationships among arthropods remain contentious because morphological studies face challenges in resolving certain branches. Particularly difficult are relationships within and between the stem arthropods, owing largely to too few well-preserved fossil representatives. Additional fossil evidence, particularly from exceptional deposits like the Silurian Waukesha Lagerstätte in Wisconsin, helps to bolster our views on the evolutionary history of arthropods by providing well-preserved examples of novel taxa that could fit between early diverging stem-arthropod clades and modern euarthropods, thus building possible bridges between the two. Formed in karstification-induced troughs of the Manistique Formation paleoslope, the Waukesha Lagerstätte preserves a unique biota of organisms from the Telychian Age, mostly through secondary precipitation of francolite. Perhaps most well known from this deposit are the many peculiar and enigmatic arthropod taxa that could help resolve early arthropod cladistic relationships. We add to the growing body of work on the diversity, phylogeny, and taxonomic descriptions of the Waukesha biota by detailing a previously unnamed bivalved arthropod, informally called ‘the butterfly animal’ in past literature—which we here designate as Papiliomaris kluessendorfae n. gen. n. sp. We also conducted a Bayesian phylogenetic analysis that placed several recently described Waukesha taxa as basal members of the ‘Mandibulate’ clade within the Euarthropoda.

UUID: http://zoobank.org/c1d6f1dc-d35f-420c-aa57-2c8983f9779e

Sulforaphane (SFN), a bioactive compound derived from glucoraphanin in cruciferous vegetables such as broccoli, has been extensively studied for its therapeutic potential across diverse disease categories. SFN exerts its effects through well-characterised pathways, including the Keap1/Nrf2 axis, which regulates phase II detoxification enzymes, and epigenetic mechanisms such as histone deacetylase inhibition. This review evaluates clinical trials registered on ClinicalTrials.gov, focusing on those using SFN or broccoli-derived extracts.

As a result, we identified 84 trials, of which 39 have been published. Results suggest SFN’s potential in regulating redox and inflammatory pathways, improving metabolic and cardiovascular outcomes, and exerting anti-cancer and neuroprotective effects. For healthy subjects, SFN enhanced detoxification and reduced inflammation. In cancer patients, SFN showed promise in early-stage prostate and breast cancer, particularly in GSTM1-positive individuals, but had limited effects in advanced cases. For brain disorders, SFN demonstrated symptomatic improvements in autism spectrum disorder and cognitive benefits in schizophrenia but lacked robust biomarker integration. SFN had minimal impact on respiratory diseases but showed supportive roles in allergic rhinitis therapy. Metabolic disease studies revealed glycaemic control improvements in type 2 diabetes but no benefits for hypertension. Approximately 50% of completed trials remain unpublished, raising concerns about publication bias. While published results highlight SFN’s therapeutic potential, limited sample sizes and inconsistent outcomes underscore the need for more extensive, stratified trials. This review emphasises the importance of integrating mechanistic insights and precision medicine approaches to maximise SFN’s clinical utility.

Targeted sprayers use artificial intelligence to enable on-the-go weed detection and herbicide application, reducing the need to spray entire fields with foliar herbicides. A targeted sprayer was evaluated for treating weeds in corn (Zea mays L.) and soybean [Glycine max (L.) Merr.] cropping systems in the midwestern United States. Using a ONE SMART SPRAY sprayer, our objectives were to (1) evaluate the efficacy of different herbicide application programs: two-passes, spot-spray (SS) only, or simultaneous broadcast residual and SS foliar herbicides; (2) determine whether weed detection thresholds influence weed control; and (3) determine the cost for each herbicide program compared with a traditional broadcast application. Field experiments were conducted in 2022 and 2023 near Manhattan, KS, and in 2023 in Seymour, IL. Both green-on-brown (GOB; burndown applications) and green-on-green (GOG; in-crop applications) were applied. Main plot treatments consisted of four herbicide programs, and the split-plot consisted of four weed detection thresholds: herbicide Efficacy, Balanced, Savings, and a Broadcast application. The percentage of area infested with weeds within each plot was estimated visually 42 d after the GOG application. An “as-applied map” was constructed using raw sprayer data to show when nozzles were turned on or off within a subplot and used to determine herbicide program costs based on the percentage of each plot area sprayed. Results indicated that herbicide programs with simultaneous broadcast and SS components in many cases resulted in a similar area infested with weeds compared with broadcast applications with the same herbicide products. As expected, herbicide costs were lower in SS applications than in broadcast applications. The ONE SMART SPRAY sprayer demonstrated potential to reduce herbicide input costs without compromising weed control.

Saccharum barberi is regarded as a sugarcane germ plasm resource of potential value. Tissue culture serves multiple purposes in breeding-related research for sugarcane. The response to tissue culture varies considerably among sugarcane genotypes; however, the influence of genetic differences on the tissue culture performance of S. barberi had not been previously investigated. This study evaluated the genotypic variation in tissue culture response among six accessions of S. barberi. Seven parameters were assessed to determine the tissue culture performance: callus induction frequency (CIF), embryogenic callus ratio, embryogenic callus induction frequency, callus regeneration frequency, callus regeneration coefficient, overall regeneration frequency (ORF) and overall regeneration coefficient (ORC). Significant variations (P < 0.05) were observed among the S. barberi genotypes for all parameters. The broad-sense heritability ranged from 80.77% to 93.10%, indicating that genetic differences were the primary source of genotypic variation. ORF exhibited the highest diversity among the parameters, with a genotypic coefficient of variation up to 70.06%. Pansahi was identified as the most amenable genotype to tissue culture, demonstrating superior performance in both callus induction and plant regeneration. CIFs at different induction periods were strongly positively correlated with both ORF and ORC, particularly during the first week, suggesting that CIF may serve as a promising early predictor of overall regeneration competence. This study is the first to report the effect of genotypic variation on callus induction and plant regeneration of S. barberi, and the findings will be valuable for future research involving tissue culture in this species.

Norway exhibits one of the highest rates of colorectal cancer (CRC) in the world, and several dietary factors have been associated with the risk of CRC. With higher consumption of ultra-processed foods (UPF), a better understanding of how food processing affects CRC might be a new approach for prevention. The current findings are contradictory, and new findings indicate that CRC risk factors might affect colorectal subsites differently. We wanted to study the association between intake of UPF and CRC risk in Norwegian women. In this prospective cohort analysis encompassing 77 100 women (1625 cases) from the Norwegian Women and Cancer study, dietary intakes were collected using validated semi-quantitative FFQ and categorised using the Nova classification system. Multivariable Cox proportional hazard models were used to assess the association between intake of UPF and CRC risk. The average follow-up time was 17·4 years. A high UPF intake (fourth quartile), compared with a low UPF intake (first quartile), was statistically significantly associated with increased total CRC risk after adjusting for all covariates and energy intake (hazard ratio (HR) = 1·24; 95 % CI 1·04, 1·49, Pfor trend = 0·02). Furthermore, a high UPF intake, compared with a low UPF intake, was statistically significantly associated with right-sided colon cancer (HR = 1·58; 95 % CI 1·19, 2·09, Pfor trend < 0·001). More research is needed to understand the associations between UPF, UPF subgroups and total CRC as well as cancer in colorectal subsites.

Benthic gastropods are not commonly useful for biostratigraphic zonation. The gastropod genus Plocezyga is abundant throughout the marine units of the Desmoinesian through Virgilian Pennsylvanian strata in the United States. The protoconch of Plocezyga gives evidence that it had a planktonic time within its life cycle, which would give it a wider regional distribution. It has been found that Plocezyga is able to biostratigraphically zone the Pennsylvanian of the United States by use of first-occurrence range zones. The upper Atokan stage through the Virgilian Stage can be zoned using of the first occurrences of Plocezyga conica, P. excellens, P. ampla new species, P. costata, P. subquadrata, P. ornata, P. acuminata, P. obscura, and P. procera new species. The lower and upper boundary of the Desmoinesian as well as the lower boundary of the Missourian Stage are defined by these gastropod zones. Six new species are proposed: Plocezgya ampla from upper Desmoinesian units; Gamizyga lenterotunda from Missourian units; and Plocezyga pingurestis, Plocezyga procera, Cyclozyga promohumera, and Cyclozyga diversarevolvi from Virgilian units.

UUID: http://zoobank.org/d070c5ad-b65f-4ab1-842f-36058704a335

A predictive column chart was developed to assess the risk of primary liver cancer (PLC) in hepatitis B patients. Data from 107 PLC patients and 107 controls were used as the training set, with 92 patients as the validation set. An additional 446 patients from other hospitals, including 15 with PLC, formed the external validation group. Multivariate logistic regression identified gender, BMI, alcohol consumption, diabetes, family history of liver cancer, cirrhosis, and HBV DNA load as independent risk factors. The model showed strong discrimination with AUCs of 0.882 and 0.859 in the training and validation sets, respectively, and good calibration (Hosmer–Lemeshow χ² = 2.648, P = 0.954; χ² = 4.117, P = 0.846). Decision curve analysis (DCA) confirmed clinical benefit within a risk threshold of 0.07–0.95. In the external validation group, the model maintained discrimination (AUC = 0.863) and calibration (Hosmer–Lemeshow χ² = 7.999, P = 0.434), with DCA showing net benefit across 0.14–0.95. These results indicate the column chart is a reliable tool for PLC risk prediction in hepatitis B patients.

Consuming whole grains (processed cereal grains containing all the bran, germ and endosperm), and whole-grain foods such as bread, porridge and pasta made from them, is universally recognised as beneficial for health. This is consistently shown through reduced risk and incidence of chronic diseases such as CVD, type 2 diabetes and some cancers with higher whole grain and whole-grain food intakes. Despite this, and the promotion of whole-grain foods in many food-based dietary guidelines, their consumption by the majority of global populations remains below levels predicted to improve health, particularly in the UK and Ireland. This paper (a) describes how whole grains and whole-grain foods can be better identified by consumers and food manufacturers through adoption of standard definitions and food-labelling processes, (b) summarises predicted benefits associated with higher whole-grain consumption and (c) discusses how developing population-based strategies to increase whole-grain consumption can beneficially affect dietary fibre intake, using the Danish Whole-Grain Campaign as a model for success. We suggest that the forthcoming ISO definition of whole grains as a food ingredient together with conditions of use should be adopted as soon as possible in the UK and Ireland. The health benefits of consuming more whole grain are unequivocal and should be recognised by including whole grains in dietary guidance, preferably with a minimum intake level for improved health. Public Health Agencies in the UK and Ireland should work in partnership with academics, industry and retailers to raise the profile of whole grains and whole-grain foods to improve population health.

Precambrian organic-walled microfossils preserved in fine-grained sedimentary rocks constitute the earliest fossil record of eukaryotic life. The Mesoproterozoic–Neoproterozoic transition coincided with major innovations in the evolution of early eukaryotes, including the radiation of crown-group lineages, represented in these rocks by candidate red algae, green algae, and fungi. However, the diversity of these early eukaryotes is yet to be fully explored. Here, we present a systematic description of the microfossil assemblage preserved in exceptional detail within sedimentary phosphatic nodules and bands in the Diabaig Formation of the ca. 1-Ga Torridon Group of northwest Scotland. Recent work has highlighted the lacustrine or estuarine nature of its depositional environment and confirmed that these fossils may include the oldest known non-marine eukaryotes. We identified 11 morphotaxa from newly collected material, including the new genus and species Minimarmilla multicatenaria, two undoubted eukaryotes, and two probable eukaryotes. The latter include Pterospermopsimorpha sp., and a new network-forming unnamed taxon. These microfossils present an important window on eukaryotic diversification in non-marine aquatic environments during the Mesoproterozoic–Neoproterozoic.

UUID: http://zoobank.org/5829c401-04fc-4229-9136-3963275826c6

The presence of the zebrette goby (Tigrigobius zebrellus) is reported for the first time in the Canary Islands, as well as in the eastern Atlantic. A new established colony around the floating docks of the Marina Santa Cruz de Tenerife harbour has been studied. Over two hundred individuals at different developmental stages have been counted. Its restricted distribution and population characteristic show that it has an established population but with no evidence of expansion to its vicinity. Our study indicates that the population of this exotic species could be easily eradicated to avoid any possible negative impact on local benthic communities. However, if such measures are not soon implemented by the local government this study can also be used as the early invasion stage of an exotic fish species across the Canary Islands.

Guerrilla rewilding, the unsanctioned release of species into the wild, is a controversial activity criticized by most conservation professionals. In this Forum article we argue that despite this criticism, it has played a significant but underexplored role in the UK’s rewilding movement. Using examples including butterfly species, goshawk Astur gentilis, wild boar Sus scrofa, beaver Castor fiber and lynx Lynx lynx, we argue that examining these guerrilla rewilding acts provides valuable insights into public preferences for certain species, their perceived acceptability, and the ways in which they shape knowledge and practices of human–wildlife coexistence. However, our analysis also suggests that in some cases guerrilla rewilding can undermine the very species it seeks to restore. Animals released without preparation or monitoring, particularly those habituated to human presence, often lack the ability to survive independently, leading to welfare issues, human–wildlife conflict and wider ecological impacts. Furthermore, by circumventing the social and collaborative dimensions of rewilding, these actions risk deepening divisions among stakeholders, which are critical to ensuring long-term success. Nonetheless, this type of rewilding can also potentially trigger more positive emotions of recovery whilst raising the species’ profile. We find that guerrilla rewilding has in some cases influenced formal rewilding practice and the broader discourse in the UK, in stark contrast to the official government position on nature recovery. This paper draws together some key learning points and highlights areas for future research on guerrilla rewilding.

A specimen of the genus Pandeopsis Kramp, 1959 was found in surface waters off the northeast Brazilian coast at 10.93°S, 36.48°W. The specimen was assigned to the family Pandeidae on the basis of the presence of more than four hollow marginal tentacles and the absence of divided radial canals, marginal tentacles with terminal cnidocyst cluster, and oral tentacles. Among Pandeidae, the specimen was assigned to Pandeopsis due to the absence of lateral diverticula, centripetal canals, and marginal cirri as well as the presence of more than two marginal tentacles, mesenteries, smooth gonads, and manubrium with a quadrangular base. To the moment, the unique morphological trait that is likely to distinguish the two species of the genus is the presence of medusa buds in the gonads of Pandeopsis prolifera, which was not present in the specimen we found. However, as this is not a strong trait to distinguish species, we consider the present specimen to be Pandeopsis cf. ikarii. This study represents the first record of the genus in the South Atlantic Ocean.

New, well-preserved specimens of the paracrinoids Wellerocystis and Implicaticystis provide new morphological data. All specimens originate from reef facies in the Kimmswick Limestone (Upper Ordovician, Sandbian–Katian) at a single locality near St Louis, Missouri, USA. Wellerocystis is characterized by an ovoid theca largely composed of imperforate plates arranged in vertical columns lacking pore-structures but with fine granular sculpture; four recumbent branched uniserial ambulacra with up to seven branches in total; a mouth frame of four plates, one of which also contributes to the periproct frame; a sinuous hydropore; and circular gonopore. The stem is unknown; its facet is small and circular, similar to that of Platycystites. Implicaticystis is characterized by a circular, heteromorphic stem, ovoid theca composed of externally concave, perforate plates with foerstepores, internal pararhombs, and a mouth frame of three plates plus two lateral plates each bearing two facets for erect, uniserial, hemipinnate pseudoarms. Foerstepores connect to tubes that pass through the theca near plate sutures. Internal lamellae of pararhombs support thecal plates much as A-frames support ridged rooves. Erect versus recumbent and branched ambulacra evolved repeatedly in pelmatozoans so both are less useful in classifying paracrinoids than presence or absence of unique pore-structures. The sister group of paracrinoids could have included Columbocystis, rhipidocystids, and cryptocrinitids. Columbocystis is commonly mentioned in discussions in this context, but its asymmetrical facets suggest it had biserial feeding appendages, unlike uniserial paracrinoid appendages.

A highly diversified fauna of brachyurans is described from the lower Eocene Serraduy Formation of Huesca, northeastern Spain. Fifteen new and first-reported taxa of brachyurans are described including three new genera, ten new species, and five taxa in open nomenclature. New taxa include: Carpilius feldmanni n. sp., Ceronnectes rugosus n. sp., Eohexapus simplex n. sp., Galenopsis ossoi n. sp., Matutsalen rotundus n. gen. n. sp., Microboschettia elegans n. gen. n. sp., Parhalimede antiqua n. sp., Locomius parthenopimimus n. gen. n. sp., Liocarcinus tridentatus n. sp., and Xanthilites robustus n. sp. Five additional genera have been recognized from fragmented material only including Alponella sp., Lovaroides sp., Paromola sp., ?Rhinolambrus sp., and ?Spinirostrimaia sp. Moreover, based on new specimens, we provide new data on Ilerdapatiscus guardiae and Aragolambrus collinsi. New information supports previous considerations about reef settings that are comparable to modern reef ecosystems as environments of diversified decapod crustaceans in the early Eocene.

UUID: http://zoobank.org/a5426acb-1d14-4e37-a843-922c940ac5b2

During the post-harvesting process, coffee berries are dried and separated into green commercial beans and husks. The dynamics of dry matter (DM) accumulation in the berry components along the maturation process are important for the definition of the most adequate moment for the harvest, which is genotype-dependent. The DM accumulation dynamics in the berries, beans, and husks in six Coffea canephora genotypes were studied during the fruit maturation process, with the aim of identifying the fruit harvesting stage when the highest bean yield can be obtained. Berry samples were collected every two weeks at nine maturation stages starting from 33 weeks after flowering (green berry stage). Second-order polynomial regressions were used to analyse berry and bean DM accumulation over time, while temporal husk DM accumulation was compared using ANOVA and the Tukey test. DM accumulation was the highest in the berries and beans following the initial sampling, while the highest husk DM accumulation occurred at the final stages of maturation. In general, DM accumulation of all components increased as fruit maturation progressed, attaining the highest DM values in the final stages of red berries, but occurred earlier for early/medium and medium maturation cycle genotypes. The Beira Rio 8 genotype showed the highest DM accumulation in all components. Bamburral and P1 genotypes showed the lowest berry fresh mass (FM) to bean DM ratios. The A1 genotype showed the greatest berry FM to bean DM ratio, being a genotype with the lowest DM and bean mass performances and bean yield. Our data revealed that not only should the absolute berry and bean yield be considered for highly productive genotype selection but also the bean DM dynamics in the characterization of commercial coffee yield.