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The seed science community currently defines germination as radicle emergence of 2 mm from the dispersal unit. Consequently, most seed researchers abruptly terminate germination experiments after radicle emergence, concluding that the seed has germinated. However, this approach underestimates epicotyl dormancy and often leads to dormancy misclassification, or worse, a failure to identify epicotyl dormancy altogether. To address these limitations, we propose extending germination studies to the point of first leaf emergence; we term this the “full germination” period. Our methodology involves germinating fully matured, freshly collected seeds and depending on the time required for radicle emergence, the seeds are categorized into (1) viviparous, where seeds germinate prematurely while they are still attached to the parent plant or within the fruit; (2) Morphological dormancy (MD) or Non-dormant (ND), where seeds germinate within 30 days; and (3) physiological dormancy (PD) and morphophysiological dormancy (MPD), where germination does not occur within 30 days. The absence of shoot emergence within 30 days following radicle protrusion indicates the presence of epicotyl dormancy. Thus, species initially classified as ND, MD, or viviparous may be miscategorized if shoot emergence is not assessed. Likewise, seeds exhibiting PD or MPD may possess an additional epicotyl dormancy component, possibly leading to placing them in incorrect subclass or level. A comprehensive assessment of shoot development is imperative for accurate dormancy characterization. We strongly recommend monitoring seed germination until first true leaf emergence should be adopted to ensure correct conclusions about dormancy, plant life cycles and ecological adaptations.
Italian ryegrass [Lolium perenne L. ssp. multiflorum (Lam.) Husnot], a pernicious weed in wheat (Triticum aestivum L.) fields, has evolved severe resistance to the acetolactate synthase (ALS)-inhibiting herbicides, like pyroxsulam. Here, the derived cleaved amplified polymorphic sequence (dCAPS) markers were developed to detect two distinct mutations at the 197th position of the ALS gene. The method was used to examine and purify the resistant population. Homozygous populations with different 197 site mutations from the same population were obtained, and the target site–resistance mechanisms were investigated. Whole-plant dose–response bioassays show that the resistance index (RI) of the Pro-197-Thr mutant population to pyroxsulam was 508.92, whereas that of the Pro-197-Gln population was 9.75. Similar trends were observed for different herbicides within same mode of action. In vitro ALS assays demonstrated that the Pro-197-Thr population exhibited lower sensitivity to pyroxsulam than the Pro-197-Gln population, consistent with plant bioassays. Furthermore, ALS gene expression of the Pro-197-Thr population analysis is significantly higher than that in the Pro-197-Gln population, which may also explain why the Pro-197-Thr population exhibits a higher resistance level than the Pro-197-Gln population. Our findings suggest that different amino acid substitutions at one ALS gene locus can confer herbicide resistance with different levels in L. perenne ssp. multiflorum. This study provides valuable insights into the mechanisms of herbicide resistance in L. perenne ssp. multiflorum.
This study aimed to evaluate the role of oral antibiotic stepdown therapy in patients with uncomplicated streptococcal bacteremia. Streptococcus species are known pathogens in bloodstream infections (BSIs). Traditionally, BSIs were managed with intravenous (IV) antibiotics; however, growing literature supports oral antibiotics in invasive infections including BSIs.
Design:
This was a retrospective cohort study evaluating patients with streptococcal bacteremia between September 2019 and September 2021 at an academic safety-net hospital. Clinical outcomes were compared between patients completing treatment with IV antibiotics versus an oral stepdown regimen. The primary outcome, clinical failure, was a composite of BSI recurrence and infection-related readmission.
Patients:
Adult patients with at least one positive blood culture for any Streptococcus species were included. Patients with polymicrobial BSIs or complicated bacteremia were excluded.
Results:
155 patients were included, 77 (49.7%) received a course of IV antibiotics and 78 (50.3%) received an oral antibiotic stepdown regimen. Clinical failure was not different between the IV and oral groups (15.6% vs. 15.4%, respectively; OR .99 [95% CI, .41 to 2.35]). No differences were observed in 30-day all-cause mortality. Patients that received oral antibiotics had a significantly shorter hospital length of stay by 6 days (6 vs 12 d, p < .01).
Conclusions:
Our results suggest that an oral stepdown regimen for uncomplicated streptococcal BSIs is associated with similar outcomes compared to IV antibiotics. Furthermore, oral antibiotics may offer reduced length of stay and avoidance of outpatient central line placement in patients with uncomplicated streptococcal BSIs.
Party system classifications have been central in political science, especially until Sartori's influential typology in 1976. However, recent years have seen diminished attention to such classifications. Western European party systems have significantly transformed, particularly over the last 15 years due to multiple crises, affecting their core structure, or what Sartori termed ‘patterns of interparty competition.’ This raises questions about whether these changes have undermined the very concept of systemness, making classifications irrelevant. This research note redefines party systems based on the number and composition of relevant political poles (governing alternatives) and, through a long-term analysis of Western Europe (20 countries since 1945), assesses their degree of systemness. Results indicate that many systems have become ‘non-systems,’ with fluctuating and unstable party poles. Most Western European systems have exhibited this ‘non-system’ type for at least half of legislatures since 1989, thus making classifications only short-lived snapshots and inevitably useless for long-term accounts.
Increasing interdisciplinary analysis of geoarchaeological records, including sediment and ice cores, permits finer-scale contextual interpretation of the history of anthropogenic environmental impacts. In an interdisciplinary approach to economic history, the authors examine metal pollutants in a sediment core from the Roman metal-producing centre of Aldborough, North Yorkshire, combining this record with textual and archaeological evidence from the region. Finding that fluctuations in pollution correspond with sociopolitical events, pandemics and recorded trends in British metal production c. AD 1100–1700, the authors extend the analysis to earlier periods that lack written records, providing a new post-Roman economic narrative for northern England.
Recruiting and retaining racial/ethnic minorities in research remains a significant challenge, often due to mistrust in clinical research and cultural misconceptions related to specific conditions. Despite the anonymity provided by technology-based intervention studies, difficulties in participant recruitment and retention in these studies remain. This paper addresses practical issues in recruiting and retaining Asian American breast cancer survivors with pain and depressive symptoms in a technology-based intervention study.
Methods:
To identify practical issues in participant recruitment and retention, a content analysis was conducted on all recorded materials, including research diaries of individual research team members, weekly team meeting minutes, and research team members’ posts on Microsoft Teams.
Results:
Analysis identified six practical issues: (a) strict inclusion/exclusion criteria; (b) multiple stigmas associated with cancer, depressive symptoms, and pain; (c) lack of interest in research participation; (d) closed Asian American communities/groups; (e) frequent technological issues; and (f) potential unauthentic cases.
Conclusion:
Addressing these recruitment and retention issues can inform the design of future culturally tailored, technology-based intervention studies for racial and ethnic minority populations.
The food system is a major contributor to the global burden of disease, ecosystem destruction and climate change, posing considerable threats to human and planetary health and economic stability. Evidence-based food policy is fundamental to food system transformation at global, national and local or institutional levels. This study aimed to critically review the content of universities’ food sustainability policy documents.
Design:
A systematic search of higher education institutions’ policies, using targeted websites and internet searches to identify food sustainability policy documents, was conducted between May and August 2023. A quantitative content analysis of the identified documents was conducted independently by multiple researchers using a coding template. Inconsistencies in coding were subsequently checked and amended through researcher consensus.
Setting:
163 UK higher education institutions.
Participants:
N/A.
Results:
Approximately 50 % of universities had a publicly available food sustainability policy. The most common food sustainability commitments therein were communication and engagement (95·2 %), food waste (94·0 %) and quality standards and certification (91·7 %). The scope of policy commitments varied between institutions; however, comprehensive documents included multifaceted commitments tackling more than one dimension of sustainability, for example, waste mitigation strategies that tackled food insecurity through food redistribution. Few (17·9 %) policies included a commitment towards research and innovation, suggesting university operations are considered in isolation from academic and educational activities.
Conclusions:
Multifaceted policy commitments are capable of uniting numerous food-related actions and institutional activities. As such, they are likely to support food system transformation, with broader positive outcomes for the university, students and the wider community.
Pupils in alternative education provision, known as ‘Educated in Other Than At School’ (EOTAS) in Wales, UK, are among the most vulnerable learners and who, for reasons such as mental health or behavioural challenges, do not attend a mainstream or special school.
Aims
We compared self-harm, neurodevelopmental disorders and mental health conditions between EOTAS pupils and controls with similar characteristics, before and after being in EOTAS provision.
Method
This population-based electronic cohort study included pupils in Wales aged 7–18 years, from the academic years 2010–11 to 2018–19. We linked data from Education Wales to primary and secondary healthcare records within the Secure Anonymised Information Linkage (SAIL) Databank. Individuals included in the EOTAS data-set were identified as cases. Controls were pseudo-randomly selected based on equivalent age and academic year distribution.
Results
This study included 8056 pupils in EOTAS and 224 247 controls. Higher levels of deprivation, childhood maltreatment, self-harm, neurodevelopmental disorders and mental health conditions before EOTAS entry were linked to higher odds of being in EOTAS. Pupils in EOTAS provision had increased incidence of self-harm, neurodevelopmental disorders and mental health conditions, from 1 year after entering EOTAS provision up to 24 years of age, than pupils with similar characteristics not in EOTAS provision.
Conclusion
While EOTAS provision plays an important role, our findings indicate that it is not sufficient on its own to meet pupils’ social, emotional, behavioural and mental health needs. Additional support and better integration with health and social services are required.
Abstract screening, a labor-intensive aspect of systematic review, is increasingly challenging due to the rising volume of scientific publications. Recent advances suggest that generative large language models like generative pre-trained transformer (GPT) could aid this process by classifying references into study types such as randomized-controlled trials (RCTs) or animal studies prior to abstract screening. However, it is unknown how these GPT models perform in classifying such scientific study types in the biomedical field. Additionally, their performance has not been directly compared with earlier transformer-based models like bidirectional encoder representations from transformers (BERT). To address this, we developed a human-annotated corpus of 2,645 PubMed titles and abstracts, annotated for 14 study types, including different types of RCTs and animal studies, systematic reviews, study protocols, case reports, as well as in vitro studies. Using this corpus, we compared the performance of GPT-3.5 and GPT-4 in automatically classifying these study types against established BERT models. Our results show that fine-tuned pretrained BERT models consistently outperformed GPT models, achieving F1-scores above 0.8, compared to approximately 0.6 for GPT models. Advanced prompting strategies did not substantially boost GPT performance. In conclusion, these findings highlight that, even though GPT models benefit from advanced capabilities and extensive training data, their performance in niche tasks like scientific multi-class study classification is inferior to smaller fine-tuned models. Nevertheless, the use of automated methods remains promising for reducing the volume of records, making the screening of large reference libraries more feasible. Our corpus is openly available and can be used to harness other natural language processing (NLP) approaches.
The ontological complexity of the twentieth-century Cold War motivates this special issue’s investigation of how social scientists conceptualize institutional novelty and change. We begin by noting the peculiar elision of the Cold War as an explanatory mechanism in mainstream sociology, even while sociologists have theoretical tools for making sense of the phenomenon: war, schema, field, world systems, and empire. All are useful; none are sufficient. We locate the explanatory problem in a tension between notions of structure and event that has organized debate in historical social science for several scholarly generations, and offer a new intellectual tool – medium durée – as a way forward. Medium durée describes phenomena that have sufficient cohesion as ideas and relationships to endure over time, yet remain sufficiently unfixed and ambiguous as to enable multifarious action and sensemaking. Our notion of medium durée is substantially informed by the articles and commentaries assembled for this special issue, which represent three years of dialogue among the authors as well as audiences in serial panels at the 2022 and 2023 annual meetings of the Social Science History Association.
Chinese stellara (Stellera chamaejasme L.) is an indicator plant of degraded grasslands. With its robust vitality, once it emerges in grassland ecosystems, it undergoes extensive growth and rapid expansion, inevitably leading to grassland degradation. The establishment and invasion of S. chamaejasme disrupts the ecological balance of grasslands in the Qilian Mountains. This study was conducted in a grassland on the eastern slope of the Qilian Mountains, employing point pattern analysis to investigate the spatial distribution patterns of S. chamaejasme and the relationships among different age classes. The population was categorized into three growth stages: young, subadult, and mature plants. The results revealed that the spatial distribution of this population is primarily dominated by subadult plants, accounting for up to 75.48% of the total, with an overall transition trend from aggregated to random distribution. No significant spatial correlations were observed among different age groups (young, subadult, and mature plants), indicating that mature plants do not exert significant inhibitory effects on the growth of young individuals. In high-density areas, the population exhibited a distribution radiating outward from mature plants as the center, with high-density cores predominantly concentrated within 0 to 5 m. Significant density variations were observed between regions, with the highest total density estimated at approximately 9.57 plants m−2 and the lowest at 2.68 plants m−2. The invasion mechanism of S. chamaejasme is closely associated with the spatial independence of its age groups and a distribution pattern dominated by subadult plants. During the initial invasion phase, S. chamaejasme spreads predominantly around mature plants. After securing sufficient growing space (0 to 1 m), it further competes for territory through shifts in distribution patterns—transitioning from aggregated to random distribution. Additionally, significant differences in distribution density and expansion patterns across regions provide critical theoretical foundations for targeted ecological management strategies.
Folds within layered rock systems are critical for comprehending the historical processes of deformation and the rheological behaviour of rocks. The current study employs finite element modelling to investigate the development of folds in a layered rock system, with a particular focus on the impact of thinner layers on the folding of adjacent thicker layers and their subsequent interactions. The analysis indicates that harmonic folds can evolve into polyharmonic or disharmonic configurations because of the intricate interactions occurring within the contact strain zone of the thinner layer. Our numerical findings demonstrate that the geometry of folds is significantly affected by the reciprocal interactions between thinner and thicker layers, initiated by the folding of the thinner units and their consequent influence on the thicker layers, and vice versa. This dynamic interplay, however, may frequently diverge from predictions made by more simplistic models, as suggested by earlier studies. Furthermore, this research highlights the potential of utilizing higher-order fold geometries to estimate the relative viscosity between the layers and the embedded medium.
Climate conditions are known to modulate infectious disease transmission, yet their impact on measles transmission remains underexplored. In this study, we investigate the extent to which climate conditions modulate measles transmission, utilizing measles incidence data during 2005–2008 from China. Three climate-forced models were employed: a sinusoidal function, an absolute humidity (AH)-forced model, and an AH and temperature (AH/T)-forced model. These models were integrated into an inference framework consisting of a susceptible–exposed–infectious–recovered (SEIR) model and an iterated filter (IF2) to estimate epidemiological characteristics and assess climate influences on measles transmission. During the study period, measles epidemics peaked in spring in northern China and were more diverse in the south. Our analyses showed that the AH/T model better captured measles epidemic dynamics in northern China, suggesting a combined impact of humidity and temperature on measles transmission. Furthermore, we preliminarily examined the impact of other factors and found that population susceptibility and incidence rate were both positively correlated with migrant worker influx, suggesting that higher susceptibility among migrant workers may sustain measles transmission. Taken together, our study supports a role of humidity and temperature in modulating measles transmission and identifies additional factors in shaping measles epidemic dynamics in China.
Let $(X,\Delta )$ be a normal pair with a projective morphism $X \to Z$ and let A be a relatively ample $\mathbb {R}$-divisor on X. We prove the termination of some minimal model program on $(X,\Delta +A)/Z$ and the abundance conjecture for its minimal model under assumptions that the non-nef locus of $K_{X}+\Delta +A$ over Z does not intersect the non-lc locus of $(X,\Delta )$ and that the restriction of $K_{X}+\Delta +A$ to the non-lc locus of $(X,\Delta )$ is semi-ample over Z.
In recent years, the question of naturalism in the study of religions has been increasingly debated. Primarily, these discussions converge in the widely held view that naturalism is the only way for religious studies as an academic enterprise to exclude supernaturalist assumptions from its methodology. While I fully agree with this view, I argue that naturalism is usually formulated with the help of metaphysical assumptions, which are problematically embodied in the location problem, that is, the problem of how to locate certain phenomena, such as meanings and values, in the order of nature. By unfolding the dynamic between the elements of the location problem, I show that the kind of naturalism based on Wittgenstein’s thought prevents the location problem from arising and can serve as a balanced version of naturalism for use in the study of religion. While metaphysical naturalism often leads to dilemmas, within Wittgenstein’s kind of naturalism, it seems possible both to maintain anti-supernaturalism in the study of religion and to resist the metaphysical temptations hidden in our assumptions about language. These two features make Wittgenstein’s naturalism truly methodological.
Reducing crude protein in amino acid-adequate diets for broiler chickens is effective in reducing nitrogenous emissions and competition for resources between the food and feed sectors. This review provides a comprehensive analysis of the literature on the relevance of nonessential amino acids in low protein diets for broiler chickens. Glycine and serine, owing to their interconvertibility summarised as glycine equivalents (Glyequi), limit growth when dietary crude protein is reduced below 19% in up to 3-week-old birds. Considering essential amino acids and the variable Glyequi requirements enables the reduction of dietary crude protein to ∼16% without compromising growth. Variation in Glyequi requirements likely occurs predominantly from the varying amounts of uric acid formed. Other influences seem to exert lower impacts on dietary Glyequi requirements. Asparagine or glutamine is probably the growth-limiting amino acid when crude protein is reduced below 16%. Alternatively, nonspecific amino-nitrogen may be lacking in such diets. The current potential to reduce dietary crude protein when using free essential and nonessential amino acids enables to increase the efficiency of nitrogen utilisation to a value above 80%. This coincides with reduced uric acid synthesis and energy expenditure for nitrogen excretion. The lower nitrogen excretion via the urine results in a lower energy expenditure. Hence, dietary energy may prospectively be reduced once the energy-sparing effect is quantified, thereby further reducing the competition for resources between food and feed.
Scholars increasingly conceptualize populism by whether politicians use people-centric and anti-elite appeals that pit a homogeneous people against a corrupt elite. These appeals reflect “thin” ideology because they offer no programmatic content and thus politicians must pair these appeals with more substantive positions, termed their “host” (or thick) ideology, which often consists of nativism on the right (e.g., espousing anti-immigrant positions) and socialism on the left (e.g., prioritizing redistribution). An emerging literature has thus sought to estimate whether populists garner support due to their thin ideology or their substantive host ideology. To date, no research has validated whether populism treatments (1) truly operationalize populist thin ideology, and (2) do so without manipulating host ideology. Results from three conjoint validation experiments fielded in both the United States and the United Kingdom show that thin ideology treatments successfully manipulate the underlying concepts but caution that some operationalizations also affect perceptions of host ideology.
Several proposals to modernize obligations and contracts law in the Spanish Civil Code have not succeeded. However, Spanish contract law has evolved through judicial interpretation, which has reformulated existing rules and recognized new ones. This article deals with major transformations in general contract law and special contracts. Additionally, the Civil Code has been affected by its interaction with EU law, as interpreted by the CJEU. Updating the Civil Code in this manner has created conceptual obscurity and has increased legal uncertainty. Formal modernization of the Civil Code would be welcome, provided it treats Spanish private law as an integral part of the pluralistic legal order of the EU.