Aphis gossypii is one of the most economically important agricultural pests that cause serious crop losses worldwide, and the indiscriminate chemical application causes resistance development in A. gossypii, a major obstacle to successful control. In this study, we selected the up-regulated expression gene AgJHAMT, which was enriched into juvenile hormone pathway though transcriptome sequencing analysis of the cotton aphids that fed on transgenic cotton lines expressing dsAgCYP6CY3 (the TG cotton). The AgJHAMT gene was overexpressed in cotton aphids which fed on the TG cotton, and its expression profile during the nymphs was clarified. Then, silencing AgJHAMT could advance the developmental period of cotton aphids by 0.5 days compared with control groups. The T and t values of cotton aphids in the dsJHAMT treatment group (6.88 ± 0.15, 1.65 ± 0.06) were significantly shorter than that of the sprayed H2O control group (7.6 ± 0.14, 1.97 ± 0.09) (P < 0.05), respectively. The fast growth caused by AgJHAMT silencing was rescued by applying the JH analogue, methoprene. Overall, these findings clarified the function of AgJHAMT in the developmental period of A. gossypii. This study contributes to further clarify the molecular mechanisms of delaying the growth and development of cotton aphids by the transgenic cotton lines expressing dsAgCYP6CY3.

This paper investigates the composition of the internal policy advisory system (PAS) in a Napoleonic country, Italy, where policy formulation and advice have traditionally been dominated by the Ministerial Cabinets, legal competences, and with a clear influence of political parties in the selection of experts. Based on the literature on the PASs, we argue that the role of the governments in shaping the systems of advice is growing and discuss how different trends push towards a pluralization of the advisers in the Napoleonic systems. Our research undertakes a unique mapping of the internal PAS in the second Conte government (2019–2021), in order to show if the Italian PAS is becoming more plural, and who are the advisors (in terms of how varied are their characteristics, skills and mandates). Our analysis combines the descriptive mapping of the internal PAS with qualitative interviews aimed at better understanding the move from the domination of the Ministerial Cabinet towards a complex and loosely coupled network of advisors.

While tension-type headache (TTH) is the most common primary headache disorder, its effect according to sex, race and ethnicity remains unclear. We investigated disparities in sex, racial and ethnic representation in TTH clinical trials with comparison to global disease burdens. In this cross-sectional analysis, TTH clinical trials had female overrepresentation and racial and ethnic minority underrepresentation, which may affect understanding of the impact of TTH on different populations and personalized treatment development. Trial enrollment that is diverse and reflective of global disease burdens is crucial for improving study generalizability, understanding of diverse clinical presentations, and ensuring healthcare equity.

To expand on recent work, we introduce collisional terms in the analysis of the warm ion–electron, two-fluid equations for a homogeneous plasma at rest. Consequently, the plasma is now described by six variables: the magnetisation, the ratio of masses over charges, the electron and ion sound speeds, the angle between the wave vector and the magnetic field and a new parameter describing the electron–ion collision frequency. This additional parameter does not introduce new wave modes compared with the collisionless case, but does result in complex mode frequencies. Both for the backward and forward propagating modes the imaginary components are negative and thus quantify collisional damping. We provide convenient (polynomial) expressions to quantify frequencies and damping rates in all short- and long-wavelength limits, including the cutoff and resonance limits, whilst the one-fluid magnetohydrodynamic limit is retained with the familiar undamped slow, Alfvén and fast waves. As collisions only introduce a damping, the previously introduced labelling of the wave modes S, A, F, M, O and X can be kept and assigned based on their long- and short-wavelength behaviour. The obtained damping at cutoff and resonance limits is parametrised with the collision frequency, and can be tailored to match known kinetic damping expressions. It is demonstrated that varying the angle can introduce crossings between the wave modes, as was already present in the ideal ion–electron case, but also a collision frequency exceeding a critical collision frequency can lead to crossings at angles where previously only avoided crossings were found.

Teachers are grappling with increased pressure and expectations to facilitate transformative education experiences, the kinds of experiences that cultivate dispositions and skillsets essential for young peoples’ preparedness to imagine and create sustainable futures. As expectations for teachers grow, so too do initiatives intended to assist their efforts, such as the advent of classroom-ready education resources. The rise of educational resources gives cause for closer examination of how they are developed, particularly with respect to the ways they situate content in the deployment of curricular, methodological and pedagogical concepts. This article presents a practice and process of education resource creation using multi-modal content that entangles global education and conservation agendas. Through the mediating lens of UNESCO’s pillars of education, a critical discussion of the utility of these for enabling and inhibiting the articulation of a professional practice for education resource creation is offered. With the imperative for sustainability-focused education and prevalence of education resources being produced to support this, we scrutinise the importance of demystifying the professional practice of education resource creation. In doing so, we point to insights that become available when the curricular, pedagogical and methodological concepts informing education resource creation are made transparent and accessible.

Maternal diet may modulate human milk microbiota, but the effects of nutritional supplements are unknown. We examined the associations of prenatal diet and supplement use with milk microbiota composition. Mothers reported prenatal diet intake and supplement use using self-administered food frequency and standardised questionnaires, respectively. The milk microbiota was profiled using 16S rRNA gene sequencing. Associations of prenatal diet quality, dietary patterns, and supplement use with milk microbiota diversity and taxonomic structure were examined using Wilcoxon signed-rank tests and multivariable models adjusting for relevant confounders. A subset of 645 mothers participating in the CHILD Cohort Study (originally known as the Canadian Healthy Infant Longitudinal Development Study) provided one milk sample between 2 and 6 months postpartum and used prenatal multivitamin supplements ≥4 times a week. After adjusting for confounders, vitamin C supplement use was positively associated with milk bacterial Shannon diversity (β = 0.18, 95% CI = 0.05, 0.31) and Veillonella and Granulicatella relative abundance (β = 0.54; 95% CI = 0.05, 1.03 and β = 0.44; 95% CI = 0.04, 0.84, respectively), and negatively associated with Finegoldia relative abundance (β = –0.31; 95% CI = –0.63, –0.01). Fish oil supplement use was positively associated with Streptococcus relative abundance (β = 0.26; 95% CI = 0.03, 0.50). Prenatal diet quality and dietary patterns were not associated with milk microbiota composition. Prenatal vitamin C and fish oil supplement use were associated with differences in the milk microbiota composition. Future studies are needed to confirm our findings and elucidate mechanisms linking maternal supplement use to milk microbiota and child health.

We are the living. We find ourselves in a mess that is sometimes called the ‘Anthropocene’. This is a mess that has been hidden, ignored, neglected through a narrative of progress, consumption, linearity, categorisation, control, prosperity, rationality. To respond to this narrative, we employ ‘mess-making’ as a force for resistance. We rethink our more-than-human relations by concepting with mess to invigorate, agitate and provoke. Employing Haraway’s (2008) ‘messmates’, we conceptualise how ‘we’ as ecosystems of thriving life forms are constantly living, learning and dying together and need to find new ways to co-research with/in/for more-than-human methodologies. These, we suggest, are inherently messy. The paper is organised in a nonconventional way in that it is mostly created by more-than-human narratives gathered from two doctoral post-qualitative inquiries exploring play in an urban forest school in London and animal-child relations in a wall-less school in Bali. We explore how mess-making is both generative and challenging as data emerge in dynamic and exciting ways. With this messy turn, we illuminate potential for educational futures that support multispecies flourishing.

This study aimed to review and synthesize the need estimates for psychiatric beds, explore how they changed over time and compare them against the prevalence of actually existing beds. We searched PubMed, Embase classic and Embase, PsycINFO and PsycIndex, Open Grey, Google Scholar, Global Health EBSCO and Proquest Dissertations, from inception to September 13, 2022. Publications providing estimates for the required number of psychiatric inpatient beds were included. Need estimates, length of stay, and year of the estimate were extracted. Need estimates were synthesized using medians and interquartile ranges (IQRs). We also computed prevalence ratios of the need estimates and the existing bed capacities at the same time and place. Sixty-five publications with 98 estimates were identified. Estimates for bed needs were trending lower until 2000, after which they stabilized. The twenty-six most recent estimates after 2000 were submitted to data synthesis (n = 15 for beds with unspecified length of stay, n = 7 for short-stay, and n = 4 for long-stay beds). Median estimates per 100 000 population were 47 (IQR: 39 to 50) beds with unspecified length of stay, 28 (IQR: 23 to 31) beds for short-stay, and 10 (IQR: 8 to 11) for long-stay beds. The median prevalence ratio of need estimates and the actual bed prevalence was 1.8 (IQR: 1.3 to 2.3) from 2000 onwards. Historically, the need estimates for psychiatric beds have decreased until about 2000. In the past two decades, they were stable over time and consistently higher than the actual bed numbers provided.

In mammals, DNA methylation (DNAme) erasure and reinstatement during embryo development and germline establishment are sensitive to the intrauterine environment. Maternal intake of a high-fat diet (HFD), associated with excessive gestational weight gain, has transgenerational effects on offspring health, which may be mediated by changes in DNAme in the germline. Here, we tested the impact of a maternal HFD on embryonic germline DNAme erasure using a rat strain that expresses green fluorescent protein specifically in germ cells. DNAme was analysed by methyl-seq capture in germ cells collected from male and female F1 gonads at gestational day 16. Our data show that although HFD induced global hypomethylation in both sexes, DNAme erasure in female germ cells was more advanced compared to male germ cells. The delay in DNAme erasure in males and the greater impact of HFD suggest that male germ cells are more vulnerable to alterations by exogenous factors.

The floristic composition of the understory plays a fundamental role in the long-term conservation of the diversity, structure, and function of mountain cloud forests in the Andes. We evaluated the relationship between the understory tree floristic composition of four types of predefined cloud forests and the canopy structure, the light transmitted to the understory, and the effect of topography. Through multivariate analysis, we found an environmental gradient correlated with light penetration into the understory and a gradient associated with the slope and, to a lesser extent, with the elevation. Then, we identified floristically well-differentiated ecological groups in response to environmental conditions; however, the groups only partially coincided with the understory composition of the predefined forests. We found environmental response species groups such as Roupala obovata and Beilschmiedia sulcata that are indicator species of sites with lower light penetration into the understory but with steeper slopes and higher elevation. In comparison, Clusia multiflora and Zanthoxylum quinduense to be the main indicator species from sites with greater light penetration into the understory and lower slope and elevation. These findings support appropriate species selection when implementing restoration strategies in forest landscape restoration plans.

Concentrated corporate power and failures to manage the distribution of risk mean that workers bear the heaviest burden in globalised apparel supply chains. Law and associated normative frameworks seek to strengthen collective worker voice and other worker rights to tip the scales of unequal bargaining power to benefit the workers. However, some of the traditional tools of labour law such as unionising and collective bargaining have weakened over the years and exacerbated during the COVID-19 pandemic. Using a conceptual framework based on regulatory theory, feminist insights, and semi-autonomous social fields, this article examines the law-practice gap for regulating just wages within the apparel supply chain, responses, and how workers fight wage theft and carve out pathways to demand just wage standards. Drawing from the case of Sri Lanka, the article discusses how alternative forms of worker voice seek to fill in the implementation gaps. The findings of this study demonstrate worker initiatives to shape the regulation of just wages and how networked labour activism, especially by women workers, prompts to re-imagine structures of actor accountability on wage rights.

The GATT security exceptions were practically in hibernation until recently. The recent WTO disputes panel activity concerning such exceptions is characterized by a standard of review that places the accent on ‘when’ action should be taken and not so much on ‘what’ action should be taken. We see two problems with this construction. First, the ‘when’ might be a function of privileged information that those possessing it might be unwilling to divulge in a transparent manner. Second, national security is an amorphous concept, and unless we disaggregate it, it is impossible to pronounce the appropriateness of measures adopted to pursue the underlying objective. In turn, the absence of disaggregation could lead to false positives and negatives, as the same action could be pursuing essential security or providing protection to domestic players.

Cross-linguistic interactions are the hallmark of bilingual development. Theoretical perspectives highlight the key role of cross-linguistic distances and language structure in literacy development. Despite the strong theoretical assumptions, the impact of such bilingualism factors in heritage-language speakers remains elusive given high variability in children's heritage-language experiences. A longitudinal inquiry of heritage-language learners of structurally distinct languages – Spanish–English and Chinese–English bilinguals (N = 181, Mage = 7.57, measured 1.5 years apart) aimed to fill this gap. Spanish–English bilinguals showed stronger associations between morphological awareness skills across their two languages, across time, likely reflecting cross-linguistic similarities in vocabulary and lexical morphology between Spanish and English. Chinese–English bilinguals, however, showed stronger associations between morphological and word reading skills in English, likely reflecting the critical role of morphology in spoken and written Chinese word structure. The findings inform theories of literacy by uncovering the mechanisms by which bilingualism factors influence child literacy development.

The History of Economics Society (HES) is neither the oldest nor the largest academic society of historians of economic thought.1 However, no one would deny—certainly not when celebrating its fiftieth anniversary—the vital role the HES has had in establishing and shaping the field of history of economics. The goal of this paper is to go beyond mere favorable impressions to an evidence-based history grounded in analyzing the attendees of the exploratory conference in 1973 and the fifty annual HES meetings that followed.