Begonia larorum is a threatened plant species endemic to Alcatrazes Island, south-east Brazil, which had not been recorded since its discovery in the 1920s. Here we report its rediscovery after more than a century since the first and only collection. In February 2024, we found a single individual in the forest understorey and successfully propagated it ex situ. Later that year we located a population of 19 individuals in an open area of vegetation prone to fires and invasive grasses and we obtained the first colour photographs of the species. Given its restricted range and the threats to its survival, we recommend the species be assessed for inclusion on the global IUCN Red List in addition to maintaining its Critically Endangered status at national level on the Red List of Brazilian Flora. We also propose in situ and ex situ conservation actions.

The Proetida likely represent the only surviving trilobite clade past the Devonian mass extinction event(s). Although members of order Proetida have long been studied, the global phylogenetic relationships across this pivotal time are still unresolved. I used a Bayesian phylogenetic approach to construct a subordinal level tree for members within the superfamily Proetoidea. Two models, a relaxed and strict clock model, were compared and used to assess past reconstructions of clades within the order. The trees from both models highlight key relationships among proetides across the Devonian and show paraphyly in groups that have been considered monophyletic in the past. Trees from both models also suggest that major groups, e.g., the genus Gerastos Goldfuss, 1843 and the family Phillispidae (which represents the most diverse post-Devonian proetide group under current taxonomic schemes) are polyphyletic. This in turn suggests, in a paleobiological context, a more complex pattern of survivorship over the Late Devonian than previously suggested as well as pervasive parallelisms toward certain ‘Gerastos’ or ‘phillipsid’ morphotypes.

Algal beds are biodiversity hotspots in coastal ecosystems, forming complex food webs based on seaweeds. Epifauna serve as important indicators of algal productivity and environmental change, interacting closely with their host seaweeds. This study compared the composition of epifaunal assemblages associated with different seaweed species in the Noto Peninsula, Japan, to elucidate their characteristics. A survey of epifaunal communities on 13 seaweed species around Tsukumo Bay revealed significant differences in abundance and composition among species. Epifaunal abundance was particularly high in Sargassum siliquastrum and Sargassum patens. Differences in assemblage structures were observed among seaweed species and sampling sites. Fucales seaweeds harboured more diverse epifaunal communities compared with Laminariales species. Variations in epifaunal composition among taxa suggest that interactions with host seaweed species play a crucial role in shaping assemblage structure.

We investigated variations in biomass among oil palm progenies and quantified K and Mg uptake by assessing the distribution pattern of these minerals throughout plant organs, including roots and bunches. A split-plot trial was set up in which potassium chloride (KCl: 60% K2O) and kieserite (MgSO4: 27% MgO) were applied as main factors, each with three levels: 0, 1.5, 3.0 kg KCl palm˗1 year˗1; and 0, 0.75, 1.5 kg MgSO4 palm˗1 year˗1. Each fertiliser combination was applied to subplots containing four oil palm progenies (C1 to C4). Samples of oil palm organs were collected to assess their dry matter (DM) and mineral contents. Oil palm biomass was significantly different among progenies, with C3 having the highest biomass (268 kg DM palm˗1). K and Mg contents in the entire palm also varied among progenies. C2 and C3 progenies exhibited the highest amounts of K (3.81 and 3.86 kg K palm˗1, respectively), whereas C1 and C4 displayed 3.35 and 3.31 kg K palm˗1, respectively. However, C4 progeny showed the highest leaflet K concentration, revealing the dilution phenomenon and the inequal mineral distribution among palm organs. Progeny C3 had the highest Mg content (0.54 kg palm˗1) and was the most productive, exporting more K and Mg through harvest than other progenies. Our results indicate genetic variability in K and Mg uptake by oil palm, and planting materials with high K and Mg uptake efficiency would be valuable for farmers and breeders.

Colostrum (CM) and transition milk (TM) are crucial for neonatal development but knowledge about the changes in their composition in dairy goats is still limited. The aim of this study was to characterise the immunological, lipid and microbial changes from CM to TM in Guanzhong dairy goats, an indigenous, highly productive Chinese breed. Milk samples were collected from eight primiparous goats at day 0 (CM) and day 7 (TM) postpartum. Immunoglobulin concentrations (IgG, IgA, IgM) were measured by ELISA, long-chain saturated fatty acids (FA) were analysed by gas chromatography, and bacterial communities were profiled by 16S rRNA gene amplicon sequencing. Microbial functions were inferred using PICRUSt2 (Phylogenetic Investigation of Communities by Reconstruction of Unobserved States) software based on KEGG (Kyoto Encyclopedia of Genes and Genomes) orthologs. Immunoglobulin concentrations declined significantly from CM to TM, reflecting a transition from immunoprotection to food supply. The TM had increased concentrations of long-chain saturated FA, particularly palmitic acid (C16:0) and stearic acid (C18:0) and higher concentrations of branched-chain FA, suggesting an adaptation to the growing energy demands of the newborn. Although overall microbial diversity was stable, taxonomic shifts were observed. CM was enriched in Emticicia, which may be involved in oligosaccharide degradation, while TM had higher abundance of Faecalibacterium, Ruminococcus and Salinicoccus, genera associated with SCFA production and intestinal development. Functional predictions indicated enrichment of cold shock and haem biosynthetic pathways in CM and enhanced carbohydrate degradation pathways in TM. Correlation analyses revealed relationships between specific FA and bacterial genera, suggesting interactions between host, microbes and nutrients that may influence milk functionality. This integrative analysis of milk components during early lactation provides new insights into the compositional and functional dynamics of goat milk.

This communication addresses the hypothesis that silver nanoparticles obtained by green synthesis (G-AgNPs), using an aqueous extract of chicory, have antibacterial activity against bovine mastitis-associated strains and may inhibit their adherence capacity. Bovine mastitis is among the diseases with the greatest economic impact worldwide. Multifactorial in nature, it is usually associated with infection caused by bacterial species such as Staphylococcus aureus, coagulase-negative staphylococci, Streptococcus uberis, and Escherichia coli. Antibiotics are currently the main line of treatment, but in order to offset the emergence of resistance, alternative compounds and nanomaterials are being researched for prevention and control. In this study, bacteria that cause bovine mastitis were molecularly identified by groEL gene PCR-RFLP. Then, G-AgNPs were produced using an aqueous extract of chicory as a reducing and stabilizing agent. The nanoparticles showed powerful antibacterial activity, but they did not seem to reduce the adherence of pathogens to MAC-T cells that had been preincubated with a sub-MIC concentration of G-AgNPs. Nevertheless, very low concentrations (21-30 pM) were needed for the antibacterial effect to take place, which makes G-AgNPs promising candidates for the control of bovine mastitis.

Cumin (Cuminum cyminum L.) is an annual herbaceous plant from the Apiaceae family, renowned for its medicinal and culinary applications as the second most popular spice globally after black pepper. Germination is a critical stage in the life cycle of plants, particularly for medicinal plants, as it determines successful establishment and productivity. This study explores the impact of ploidy levels (diploid and tetraploid) and genotype interactions on germination traits, seed morphology and early seedling growth in five selected cumin genotypes (YAR1, KBA4, SKD6, SIV8 and NKM9). Induction of tetraploidy significantly influenced germination percentage, rate, seed vigour index, and morphological traits. Notably, diploid genotypes exhibited higher germination percentages, while tetraploid SKD6 displayed the highest germination speed and seedling biomass, demonstrating genotype-specific ploidy effects. For the first time, root growth kinetics were analyzed, revealing distinct growth patterns between diploid and tetraploid seeds. Morphometric evaluations showed that tetraploid seeds and embryos were significantly larger, attributed to the ‘gigas effect’, which enhances storage reserves and seed vigour. However, challenges such as embryo-less seeds and variability in genotype responses to ploidy manipulation were observed. These findings underscore the importance of targeted breeding strategies that optimize genotype-ploidy interactions to improve seed quality, germination performance and early growth in cumin. By advancing our understanding of polyploidy’s role in shaping key agronomic traits, this study provides a foundation for sustainable cultivation practices and enhanced productivity of medicinal plants.