Klotho is a protein that plays different functions in female fertility. We have previously reported that klotho protein supplementation during in vitro maturation improves porcine embryo development, while klotho knockout for somatic cell cloning completely blocks full-term pregnancy in vivo. However, the effects of the microinjection of klotho protein or klotho knockdown dual vector in porcine embryos at different time points and the specific molecular mechanisms remain largely unknown. In this study, we injected the preassembled cas9 + sgRNA dual vector, for klotho knockdown, into the cytoplasm of the germinal vesicle stage of oocytes and into porcine embryos after 6-h parthenogenetic activation. Similarly, the klotho protein was inserted into the cytoplasm of germinal vesicle stage oocytes and porcine embryos after 6-h parthenogenetic activation. Compared with the controls, the microinjection of klotho dual vector markedly decreased the blastocyst formation rates in germinal vesicle stage oocytes and activated embryos. However, the efficiency of blastocyst formation when klotho protein was inserted before in vitro maturation was significantly higher than that after klotho protein insertion into parthenogenetically activated embryos. These results indicated that klotho knockdown may impair embryo development into blastocyst irrespective of injection timing. In addition, klotho protein injection timing in pig embryos may be an important factor for regulating embryo development.

Globally, diet quality is poor, with populations failing to achieve national dietary guidelines. Such failure has been consistently linked with malnutrition and poorer health outcomes. In addition to the impact of diet on health outcomes, it is now accepted that what we eat, and the resulting food system, has significant environmental or planetary health impacts. Changes are required to our food systems to reduce these impacts and mitigate the impact of climate change on our food supply. Given the complexity of the interactions between climate change, food and health, and the different actors and drivers that influence these, a systems-thinking approach to capture such complexity is essential. Such an approach will help address the challenges set by the UN 2030 Agenda for sustainable development in the form of the sustainable development goals (SDG). Progress against SDG has been challenging, with an ultimate target of 2030. While the scientific uncertainties regarding diet and public and planetary health need to be addressed, equal attention needs to be paid to the structures and systems, as there is a need for multi-level, coherent and sustained structural interventions and policies across the full food system/supply chain to effect behaviour change. Such systems-level change must always keep nutritional status, including impact on micronutrient status, in mind. However, benefits to both population and environmental health could be expected from achieving dietary behaviour change towards more sustainable diets.

We will present the proof of existence and uniqueness of renormalized solutions to a broad family of strongly non-linear elliptic equations with lower order terms and data of low integrability. The leading part of the operator satisfies general growth conditions settling the problem in the framework of fully anisotropic and inhomogeneous Musielak–Orlicz spaces. The setting considered in this paper generalized known results in the variable exponents, anisotropic polynomial, double phase and classical Orlicz setting.

Accelerator mass spectrometry (AMS) radiocarbon (14C) dating is central to the development of robust chronologies in archaeological and paleoenvironmental contexts spanning the last 50,000 years. For dates to be accurate, samples must be free of exogenous carbon contamination. At the Oxford Radiocarbon Accelerator Unit (ORAU), considerable advancements in the dating of bone collagen have been made through the development of a high performance liquid chromatography (HPLC) method for the dating of the amino acid hydroxyproline, which can mitigate the effects of carbon contamination. However, recent changes in ligand manufacturing methods for the mixed-mode column used in the ORAU protocol (Primesep A, SIELC Technologies; IL, USA) have resulted in unacceptably high analytical backgrounds. Prior to the manufacturing change, backgrounds of > 50k BP were achievable. Since the manufacturing change, a mean background of 32.5k BP has been measured. Due to column bleed, the Primesep A is therefore no longer suitable for 14C measurement of hydroxyproline from older material. Here, we present background data and the chromatography conditions used to isolate hydroxyproline using an alternative column, a preparative-scale Newcrom AH, which shows promising potential as an alternative for the routine isolation and AMS dating of hydroxyproline—especially approaching the age and mass limits of the method.

In 1653, the Congregation for the Propagation of the Faith created a secular mission for Scotland that initially struggled with finances and provision. Its Prefects argued that the Jesuits exacerbated these problems by failing to prepare students at the Scots College Rome intellectually for the mission. This article examines the resulting campaign for curricular reform that Scottish secular priests waged, one intended to improve missionaries’ pastoral skills and undermine the college's Jesuit administration. It ultimately demonstrates the significance of education to wider conflicts between Propaganda Fide and the Society of Jesus regarding missions and resources in the seventeenth century.

Why do racialised states subscribe to the racial international hierarchy? While the critical scholarship in International Relations (IR) has meaningfully unsettled the discipline’s silence on race, it remains bound to the white versus non-white binary, neglecting the transmission and persistence of racism in international politics outside that divide. This article proposes a Lacanian reading of race as constitutive of state subjectivity in the modern world order to address this gap. Focusing on Lacan’s notion of the ‘lack in the Other’, I suggest that non-West/non-white racism is a fantasy that racialised states construct upon encountering the void of ‘Whiteness’ as a master signifier. I argue that racialised states appropriate racism in response to the anxiety induced by the collapse of the Other’s authority. Using the case of Japan’s transition to a modern nation-state, I mobilise the framework to examine Japan’s flirtation with Western racial theories and subsequent attempts to depart from the white racial order by creating its own racial hierarchy.

Sleep deprivation, which is a decrease in duration and quality of sleep, is a common problem in today’s life. Epidemiological and interventional investigations have suggested a link between sleep deprivation and overweight/obesity. Sleep deprivation affects homeostatic and non-homoeostatic regulation of appetite, with the food reward system playing a dominant role. Factors such as sex and weight status affect this regulation; men and individuals with excess weight seem to be more sensitive to reward-driven and hedonistic regulation of food intake. Sleep deprivation may also affect weight through affecting physical activity and energy expenditure. In addition, sleep deprivation influences food selection and eating behaviours, which are mainly managed by the food reward system. Sleep-deprived individuals mostly crave for palatable energy-dense foods and have low desire for fruit and vegetables. Consumption of meals may not change but energy intake from snacks increases. The individuals have more desire for snacks with high sugar and saturated fat content. The relationship between sleep and the diet is mutual, implying that diet and eating behaviours also affect sleep duration and quality. Consuming healthy diets containing fruit and vegetables and food sources of protein and unsaturated fats and low quantities of saturated fat and sugar may be used as a diet strategy to improve sleep. Since the effects of sleep deficiency differ between animals and humans, only evidence from human subject studies has been included, controversies are discussed and the need for future investigations is highlighted.

We find firm cyclicality decreases by 40% after the inception of credit default swap (CDS) trading. The effect stems from CDS firms’ less aggressive asset growth in good times and is stronger for firms facing a more severe empty creditor problem. Important identification issues are addressed. The result cannot be explained with debt overhang, bank lending cyclicality, or the cyclicality of firms’ business fundamentals. It holds for the cyclicality of various corporate outcomes (inventories, cash, and employment). Importantly, CDS trading impedes unhealthy growth and enhances profitability and firm value. Our finding indicates an important positive real effect of financial innovation.

This paper is concerned with the relationship of $y$-smooth integers and de Bruijn's approximation $\Lambda (x,\,y)$. Under the Riemann hypothesis, Saias proved that the count of $y$-smooth integers up to $x$, $\Psi (x,\,y)$, is asymptotic to $\Lambda (x,\,y)$ when $y \ge (\log x)^{2+\varepsilon }$. We extend the range to $y \ge (\log x)^{3/2+\varepsilon }$ by introducing a correction factor that takes into account the contributions of zeta zeros and prime powers. We use this correction term to uncover a lower order term in the asymptotics of $\Psi (x,\,y)/\Lambda (x,\,y)$. The term relates to the error term in the prime number theorem, and implies that large positive (resp. negative) values of $\sum _{n \le y} \Lambda (n)-y$ lead to large positive (resp. negative) values of $\Psi (x,\,y)-\Lambda (x,\,y)$, and vice versa. Under the Linear Independence hypothesis, we show a Chebyshev's bias in $\Psi (x,\,y)-\Lambda (x,\,y)$.

Indigenous peoples continue to challenge Canadian colonial policies through nonroutine acts of resistance. Sustained scholarly attention on the frequency and characteristics of Indigenous resistance has dropped precipitously, with the time span of this scholarship typically ending by the early 2000s. Research on more recent acts of resistance is directed to small-n case studies. This research note examines Indigenous resistance in Canada between 2010 and 2020 as reported by news articles from the Canadian Broadcasting Corporation (CBC) to identify key characteristics of salient Indigenous resistance: What issue areas were the subject of resistance movements? Which Indigenous communities or groups contributed to acts of resistance? What strategies were employed? The research note's findings suggest that salient acts of resistance rarely result in immediate policy change from the state; instead, resistance has transformative potential to develop Indigenous governance that departs from settler-colonial state processes.

This experiment was conducted to investigate whether dietary chenodeoxycholic acid (CDCA) could attenuate high-fat (HF) diet-induced growth retardation, lipid accumulation and bile acid (BA) metabolism disorder in the liver of yellow catfish Pelteobagrus fulvidraco. Yellow catfish (initial weight: 4·40 (sem 0·08) g) were fed four diets: the control (105·8 g/kg lipid), HF diet (HF group, 159·6 g/kg lipid), the control supplemented with 0·9 g/kg CDCA (CDCA group) and HF diet supplemented with 0·9 g/kg CDCA (HF + CDCA group). CDCA supplemented in the HF diet significantly improved growth performance and feed utilisation of yellow catfish (P < 0·05). CDCA alleviated HF-induced increment of hepatic lipid and cholesterol contents by down-regulating the expressions of lipogenesis-related genes and proteins and up-regulating the expressions of lipololysis-related genes and proteins. Compared with the control group, CDCA group significantly reduced cholesterol level (P < 0·05). CDCA significantly inhibited BA biosynthesis and changed BA profile by activating farnesoid X receptor (P < 0·05). The contents of CDCA, taurochenodeoxycholic acid and glycochenodeoxycholic acid were significantly increased with the supplementation of CDCA (P < 0·05). HF-induced elevation of cholic acid content was significantly attenuated by the supplementation of CDCA (P < 0·05). Supplementation of CDCA in the control and HF groups could improve the liver antioxidant capacity. This study proved that CDCA could improve growth retardation, lipid accumulation and BA metabolism disorder induced by HF diet, which provided new insight into understanding the physiological functions of BA in fish.