We use cookies to distinguish you from other users and to provide you with a better experience on our websites. Close this message to accept cookies or find out how to manage your cookie settings.
To save content items to your account,
please confirm that you agree to abide by our usage policies.
If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your account.
Find out more about saving content to .
To save content items to your Kindle, first ensure no-reply@cambridge.org
is added to your Approved Personal Document E-mail List under your Personal Document Settings
on the Manage Your Content and Devices page of your Amazon account. Then enter the ‘name’ part
of your Kindle email address below.
Find out more about saving to your Kindle.
Note you can select to save to either the @free.kindle.com or @kindle.com variations.
‘@free.kindle.com’ emails are free but can only be saved to your device when it is connected to wi-fi.
‘@kindle.com’ emails can be delivered even when you are not connected to wi-fi, but note that service fees apply.
With increased global navigation satellite system (GNSS) signals and degraded observation environments, the correctness of ambiguity resolution is disturbed, causing unexpected real-time kinematic (RTK) positioning solutions. This paper presents an improved fault detection and exclusion (FDE) method based on the generalized least squares (GLS) model. The correlated GLS model is constructed by regarding double-differencing (DD) integer ambiguities as the known parameters. Meanwhile, the validity of residuals as crucial components of fault detection could be enhanced by the iterative re-weighted least squares (IRLS) method rather than the least squares (LS) without robustness. A static test with artificial faults and a dynamic test with natural faults were carried out, respectively. By analyzing test statistics of the enhanced FDE algorithm and comparing its positioning errors with those from the classical LS, it is shown that our method can provide high-precision and high-reliability RTK solutions facing wrong DD fixed ambiguities due to observation faults.
Tumour immunotherapy holds great promise as a treatment for cancer, which ranks as the second highest cause of mortality worldwide. This therapeutic approach can be broadly categorized into two main types: active immunotherapy and passive or adoptive immunotherapy. Active immunotherapy, such as cancer vaccines, stimulates the patients’ immune system to target tumour cells. On the other hand, adoptive immunotherapy involves supplying in vitro activated immune cells, such as T cells, natural killer cells and macrophages, to the patient to combat the tumour. Induced pluripotent stem cells are extensively utilized in both active and adoptive tumour immunotherapy due to their pluripotency and ease of gene editing. They can be differentiated into various types of immune cells for direct cancer treatment and can also function as tumour vaccines to elicit an immune response against the tumour. Importantly, iPSCs can be leveraged to develop off-the-shelf allogenic immunotherapy products.
Conclusion
This article provides a comprehensive review of the application of iPSCs in tumor immunotherapy, along with a discussion of the opportunities and challenges in this evolving field.
The Chinese pangolin Manis pentadactyla is categorized as Critically Endangered on the IUCN Red List, but the development of effective conservation strategies is hindered by a lack of data on its distribution range and population dynamics. In addition, standardized survey and analysis methods are required to facilitate the sharing of results and maximize conservation effectiveness. To fill these knowledge and methodological gaps, we investigated the occurrence of pangolin burrows in the subtropical forest ecosystem of Fujian, China. We surveyed a total of 70 transects across five land-cover types within the Fujian Junzifeng National Nature Reserve and detected 87 burrows. The majority of burrows (87%) were located in mixed conifer and broadleaf forests. We used six environmental variables in a generalized linear model to examine the relationship between the occurrence of burrows and environmental factors. The average model results from the best model set showed that the distribution of burrows was significantly influenced by forest type. For effective pangolin conservation, we recommend that local conservation authorities prioritize the protection of mixed conifer and broadleaf forests. Our findings support the local conservation of the Chinese pangolin and the standardization of surveys and conservation efforts across the species’ range.
In small-plot experiments, weed scientists have traditionally estimated herbicide efficacy through visual assessments or manual counts with wooden frames—methods that are time-consuming, labor-intensive, and error-prone. This study introduces a novel mobile application (app) powered by convolutional neural networks (CNNs) to automate the evaluation of weed coverage in turfgrass. The mobile app automatically segments input images into 10 by 10 grid cells. A comparative analysis of EfficientNet, MobileNetV3, MobileOne, ResNet, ResNeXt, ShuffleNetV1, and ShuffleNetV2 was conducted to identify weed-infested grid cells and calculate weed coverage in bahiagrass (Paspalum notatum Flueggé), dormant bermudagrass [Cynodon dactylon (L.) Pers.], and perennial ryegrass (Lolium perenne L.). Results showed that EfficientNet and MobileOne outperformed other models in detecting weeds growing in bahiagrass, achieving an F1 score of 0.988. For dormant bermudagrass, ResNet performed best, with an F1 score of 0.996. Additionally, app-based coverage estimates (11%) were highly consistent with manual assessments (11%), showing no significant difference (P = 0.3560). Similarly, ResNeXt achieved the highest F1 score of 0.996 for detecting weeds growing in perennial ryegrass, with app-based and manual coverage estimates also closely aligned at 10% (P = 0.1340). High F1 scores across all turfgrass types demonstrate the models’ ability to accurately replicate manual assessments, which is essential for herbicide efficacy trials requiring precise weed coverage data. Moreover, the time for weed assessment was compared, revealing that manual counting with 10 by 10 wooden frames took an average of 39.25, 37.25, and 42.25 s per instance for bahiagrass, dormant bermudagrass, and perennial ryegrass, respectively, whereas the app-based approach reduced the assessment times to 8.23, 7.75, and 14.96 s, respectively. These results highlight the potential of deep learning–based mobile tools for fast, accurate, scalable weed coverage assessments, enabling efficient herbicide trials and offering labor and cost savings for researchers and turfgrass managers.
In this paper, we propose a novel online informative path planner for 3-D modeling of unknown structures using micro aerial vehicles. Different from the explore-then-exploit strategy, our planner can cope with exploration and coverage simultaneously and thus obtain complete and high-quality 3-D models. We first devise a set of evaluation metrics considering the perception constraints of the sensor for efficiently evaluating the coverage quality of the reconstructed surfaces. Then, the coverage quality is utilized to guide the subsequent informative path planning. Specifically, our hierarchical planner consists of two planning stages – a local coverage stage for inspecting surfaces with low coverage quality and a global exploration stage for transiting the robot to unexplored regions at the global scale. The local coverage stage computes the coverage path that takes into account both the exploration and coverage objectives based on the estimated coverage quality and frontiers, and the global exploration stage maintains a sparse roadmap in the explored space to achieve fast global exploration. We conduct both simulated and real-world experiments to validate the proposed method. The results show that our planner outperforms the state-of-the-art algorithms and especially decreases the reconstruction error (at least 12.5% lower on average).
To investigate the effects of activating/inhibiting AmelSmo on the olfactory genes and signalling pathways of Apis mellifera ligustica, as well as the potential regulatory mechanisms involved. Transcriptomic sequencing was performed on Apis mellifera ligustica antennae using Illumina HiSeq platform following administration of cyclopamine (inhibitor) and purmorphamine (agonist). Differential gene expression analysis, coupled with GO and KEGG pathway annotations, facilitated the identification of olfactory receptor genes. The reliability of transcriptome data was subsequently validated through quantitative real–time–polymerase chain reaction analysis. Transcriptomic analysis revealed 12,356 differentially expressed genes (DEGs) between inhibitor and control groups, with 276 genes showing significant differential expression. Similarly, 12,356 DEGs were identified between the agonist and control groups, among which 672 genes exhibited significant differential expression. The GO annotation revealed that the DEGs in the inhibitor group and the agonist group were mainly enriched in the biological process such as cellular process, metabolic process, and biological regulation; in cellular component, enrichment was mainly observed in cell, cell part, and organelle; and in molecular function, the main enrichment was in binding and catalytic activity. KEGG pathway analysis indicated that DEGs from both groups were primarily enriched in signal transduction pathways. Among the DEGs, three olfactory receptor genes were identified in the inhibitor group: odorant receptor 19, odorant receptor 22, and odorant receptor 5. The agonist group exhibited two olfactory receptor genes: odorant receptor 109 and odorant receptor 26. All these olfactory receptor genes demonstrated downregulated expression patterns. Transcriptomic sequencing analysis identified five olfactory receptor genes. The changes in gene expression levels suggest that the activation or inhibition of AmelSmo may regulate the expression of olfactory receptors via the Hedgehog signalling pathway. It is speculated that AmelSmo may play a regulatory role in the olfactory system of bees.
Depression is closely associated with abnormalities in brain function. Traditional static functional connectivity analyses offer limited insight into the temporal variability of brain activity. Recent advances in dynamic analyses enable a deeper understanding of how depression relates to temporal fluctuations in brain activity.
Methods
This study utilized a large resting-state functional magnetic resonance imaging dataset (N = 696) to examine the association between brain dynamics and depression. Two complementary approaches were employed. Hidden Markov modeling (HMM) was used to identify discrete brain states and quantify their temporal switching patterns; temporal variability was computed within and between large-scale functional networks to capture time-varying fluctuations in functional connectivity.
Results
Depression scores were positively associated with switching rate and negatively associated with maximum fractional occupancy. Furthermore, depression scores were significantly associated with greater temporal variability both within and between networks, with particularly strong effects observed in the default mode network, ventral attention network, and frontoparietal network. Together, these findings suggest that individuals with higher depression scores exhibit more unstable brain dynamics.
Conclusion
Our findings reveal that individuals with higher depression levels exhibit greater instability in brain state transitions and increased temporal variability in functional connectivity across large-scale networks. This instability in brain dynamics may contribute to difficulties in emotion regulation and cognitive control. By capturing whole-brain temporal patterns, this study offers a novel perspective on the neural basis of depression.
Tuberculosis (TB) remains a significant public health concern in China. Using data from the Global Burden of Disease (GBD) study 2021, we analyzed trends in age-standardized incidence rate (ASIR), prevalence rate (ASPR), mortality rate (ASMR), and disability-adjusted life years (DALYs) for TB from 1990 to 2021. Over this period, HIV-negative TB showed a marked decline in ASIR (AAPC = −2.34%, 95% CI: −2.39, −2.28) and ASMR (AAPC = −0.56%, 95% CI: −0.62, −0.59). Specifically, drug-susceptible TB (DS-TB) showed reductions in both ASIR and ASMR, while multidrug-resistant TB (MDR-TB) showed slight decreases. Conversely, extensively drug-resistant TB (XDR-TB) exhibited upward trends in both ASIR and ASMR. TB co-infected with HIV (HIV-DS-TB, HIV-MDR-TB, HIV-XDR-TB) showed increasing trends in recent years. The analysis also found an inverse correlation between ASIRs and ASMRs for HIV-negative TB and the Socio-Demographic Index (SDI). Projections from 2022 to 2035 suggest continued increases in ASIR and ASMR for XDR-TB, HIV-DS-TB, HIV-MDR-TB, and HIV-XDR-TB. The rising burden of XDR-TB and HIV-TB co-infections presents ongoing challenges for TB control in China. Targeted prevention and control strategies are urgently needed to mitigate this burden and further reduce TB-related morbidity and mortality.
This study aimed to examine the relationship between FGF19 and depressive symptoms, measured by BDI scores and investigate the moderating role of smoking.
Methods:
This study involved 156 Chinese adult males (78 smokers and 78 non-smokers) from September 2014 to January 2016. The severity of depressive symptoms was evaluated using the BDI scores. Spearman rank correlation analyses were used to investigate the relationship between CSF FGF19 levels and BDI scores. Additionally, moderation and simple slope analyses were applied to assess the moderating effect of smoking on the relationship between the two.
Results:
FGF19 levels were significantly associated with BDI scores across all participants (r = 0.26, p < 0.001). Smokers had higher CSF FGF19 levels and BDI scores compared to non-smokers (445.9 ± 272.7 pg/ml vs 229.6 ± 162.7 pg/ml, p < 0.001; 2.7 ± 3.0 vs 1.3 ± 2.4, p < 0.001). CSF FGF19 levels were positively associated with BDI scores in non-smokers (r = 0.27, p = 0.015), but no similar association was found among smokers (r = -0.11, p = 0.32). Linear regression revealed a positive correlation between FGF19 and BDI scores (β = 0.173, t = 2.161, 95% CI: 0.015- 0.331, p < 0.05), which was negatively impacted by smoking (β = -0.873, t = -4.644, 95% CI: -1.244 to -0.501, p < 0.001).
Conclusion:
These results highlight the potential role of FGF19 in individuals at risk for presence of or further development of depressive symptoms and underscore the importance of considering smoking status when examining this association.
This study investigated the factors influencing the mental health of rural doctors in Hebei Province, to provide a basis for improving the mental health of rural doctors and enhancing the level of primary health care.
Background:
The aim of this study was to understand the mental health of rural doctors in Hebei Province, identify the factors that influence it, and propose ways to improve their psychological status and the level of medical service of rural doctors.
Methods:
Rural doctors from 11 cities in Hebei Province were randomly selected, and their basic characteristics and mental health status were surveyed via a structured questionnaire and the Symptom Checklist-90 (SCL-90). The differences between the SCL-90 scores of rural doctors in Hebei Province and the Chinese population norm, as well as the proportion of doctors with mental health problems, were compared. Logistic regression was used to analyse the factors that affect the mental health of rural doctors.
Results:
A total of 2593 valid questionnaires were received. The results of the study revealed several findings: the younger the rural doctors, the greater the incidence of mental health problems (OR = 0.792); female rural doctors were more likely to experience mental health issues than their male counterparts (OR = 0.789); rural doctors with disabilities and chronic diseases faced a significantly greater risk of mental health problems compared to healthy rural doctors (OR = 2.268); rural doctors with longer working hours have a greater incidence of mental health problems; and rural doctors with higher education backgrounds have a higher prevalence of somatization (OR = 1.203).
Conclusion:
Rural doctors who are younger, male, have been in medical service longer, have a chronic illness or disability, and have a high degree of education are at greater risk of developing mental health problems. Attention should be given to the mental health of the rural doctor population to improve primary health care services.
Compelling evidence claims that gut microbial dysbiosis may be causally associated with major depressive disorder (MDD), with a particular focus on Alistipes. However, little is known about the potential microbiota–gut–brain axis mechanisms by which Alistipes exerts its pathogenic effects in MDD.
Methods
We collected data from 16S rDNA amplicon sequencing, untargeted metabolomics, and multimodal brain magnetic resonance imaging from 111 MDD patients and 102 healthy controls. We used multistage linked analyses, including group comparisons, correlation analyses, and mediation analyses, to explore the relationships between the gut microbiome (Alistipes), fecal metabolome, brain imaging, and behaviors in MDD.
Results
Gut microbiome analysis demonstrated that MDD patients had a higher abundance of Alistipes relative to controls. Partial least squares regression revealed that the increased Alistipes was significantly associated with fecal metabolome in MDD, involving a range of metabolites mainly enriched for amino acid, vitamin B, and bile acid metabolism pathways. Correlation analyses showed that the Alistipes-related metabolites were associated with a wide array of brain imaging measures involving gray matter morphology, spontaneous brain function, and white matter integrity, among which the brain functional measures were, in turn, associated with affective symptoms (anxiety and anhedonia) and cognition (sustained attention) in MDD. Of more importance, further mediation analyses identified multiple significant mediation pathways where the brain functional measures in the visual cortex mediated the associations of metabolites with behavioral deficits.
Conclusion
Our findings provide a proof of concept that Alistipes and its related metabolites play a critical role in the pathophysiology of MDD through the microbiota–gut–brain axis.
We sought to assess the degree to which environmental risk factors affect CHD prevalence using a case–control study.
Methods:
A hospital-based study was conducted by collecting data from outpatients between January 2016 and January 2021, which included 31 CHD cases and 72 controls from eastern China. Risk ratios were estimated using univariate and multivariate logistic regression models and mediating effect analysis.
Results:
Residential characteristics (usage of cement flooring, odds ratio = 17.04[1.954–148.574], P = 0.01; musty smell, odds ratio = 3.105[1.198–8.051], P = 0.02) and indoor total volatile organic compound levels of participants’ room (odds ratio = 31.846[8.187–123.872, P < 0.001), benzene level (odds ratio = 7.370[2.289–23.726], P = 0.001) increased the risk of CHDs in offspring. And folic acid plays a masking effect, which mitigates the affection of the total volatile organic compound (indirect effect = -0.072[−0.138,-0.033]) and formaldehyde (indirect effect = −0.109[-0.381,-0.006]) levels on the incidence of CHDs. While food intake including milk (odds ratio = 0.396[0.16–0.977], P = 0.044), sea fish (odds ratio = 0.273[0.086–0.867], P = 0.028), and wheat (odds ratio = 0.390[0.154–0.990], P = 0.048) were all protective factors for the occurrence of CHDs. Factors including women reproductive history (history of conception control, odds ratio = 2.648[1.062–6.603], P = 0.037; history of threatened abortion, odds ratio = 2.632[1.005–6.894], P = 0.049; history of dysmenorrhoea (odds ratio = 2.720[1.075–6.878], P = 0.035); sleep status (napping habit during daytime, odds ratio = 0.856[0.355–2.063], P = 0.047; poor sleep quality, odds ratio = 3.180[1.037–9.754], P = 0.043); and work status (working time > 40h weekly, odds ratio = 2.882[1.172–7.086], P = 0.021) also influenced the CHDs incidence to differing degrees.
Conclusion:
Diet habits, nutrients intake, psychological status of pregnant women, and residential air quality were associated with fetal CHDs. Indoor total volatile organic compound content was significantly correlated with CHDs risk, and folic acid may serve as a masking factor that reduce the harmful effects of air pollutants.
Turbulent mixing driven by the reshocked Richtmyer–Meshkov (RM) instability plays a critical role in numerous natural phenomena and engineering applications. As the most fundamental physical quantity characterizing the mixing process, the mixing width transitions from linear to power-law growth following the initial shock. However, there is a notable absence of quantitative models for predicting the pronounced compression of initial interface perturbations or mixing regions at the moment of shock impact. This gap has restricted the development of integrated algebraic models to only the pre- and post-shock evolution stages. To address this limitation, the present study develops a predictive model for the compression of the mixing width induced by shocks. Based on the general principle of growth rate decomposition proposed by Li et al. (Phy. Rev. E, vol. 103, issue 5, 2021, 053109), two distinct types of shock-induced compression processes are identified, differentiated by the dominant mechanism governing their evolution: light–heavy and heavy–light shock-induced compression. For light–heavy interactions, both stretching (compression) and penetration mechanisms are influential, whereas heavy–light interactions are governed predominantly by the stretching (compression) mechanism. To characterize these mechanisms, the average velocity difference between the extremities of the mixing zone is quantified, and a physical model of RM mixing is utilized. A quantitative theoretical model is subsequently formulated through the independent algebraic modelling of these two mechanisms. The proposed model demonstrates excellent agreement with numerical simulations of reshocked RM mixing, offering valuable insights for the development of integrated algebraic models for mixing width evolution.
This study presents a novel investigation into the vortex dynamics of flow around a near-wall rectangular cylinder based on direct numerical simulation at $Re=1000$, marking the first in-depth exploration of these phenomena. By varying aspect ratios ($L/D = 5$, $10$, $15$) and gap ratios ($G/D = 0.1$, $0.3$, $0.9$), the study reveals the vortex dynamics influenced by the near-wall effect, considering the incoming laminar boundary layer flow. Both $L/D$ and $G/D$ significantly influence vortex dynamics, leading to behaviours not observed in previous bluff body flows. As $G/D$ increases, the streamwise scale of the upper leading edge (ULE) recirculation grows, delaying flow reattachment. At smaller $G/D$, lower leading edge (LLE) recirculation is suppressed, with upper Kelvin–Helmholtz vortices merging to form the ULE vortex, followed by instability, differing from conventional flow dynamics. Larger $G/D$ promotes the formation of an LLE shear layer. An intriguing finding at $L/D = 5$ and $G/D = 0.1$ is the backward flow of fluid from the downstream region to the upper side of the cylinder. At $G/D = 0.3$, double-trailing-edge vortices emerge for larger $L/D$, with two distinct flow behaviours associated with two interactions between gap flow and wall recirculation. These interactions lead to different multiple flow separations. For $G/D = 0.9$, the secondary vortex (SV) from the plate wall induces the formation of a tertiary vortex from the lower side of the cylinder. Double-SVs are observed at $L/D = 5$. Frequency locking is observed in most cases, but is suppressed at $L/D = 10$ and $G/D = 0.9$, where competing shedding modes lead to two distinct evolutions of the SV.
Two-dimensional simulations incorporating detailed chemistry are conducted for detonation initiation induced by dual hot spots in a hydrogen/oxygen/argon mixture. The objective is to examine the transient behaviour of detonation initiation as facilitated by dual hot spots, and to elucidate the underlying mechanisms. Effects of hot spot pressure and distance on the detonation initiation process are assessed; and five typical initiation modes are identified. It is found that increasing the hot spot pressure promotes detonation initiation, but the impact of the distance between dual hot spots on detonation initiation is non-monotonic. During the initiation process, the initial hot spot autoignites, and forms the cylindrical shock waves. Then, the triple-shock structure, which is caused by wave collisions and consists of the longitudinal detonation wave, transverse detonation wave and cylindrical shock wave, dominates the detonation initiation behaviour. A simplified theoretical model is proposed to predict the triple-point path, whose curvature quantitatively indicates the diffraction intensity of transient detonation waves. The longitudinal detonation wave significantly diffracts when the curvature of the triple-point path is large, resulting in the failed detonation initiation. Conversely, when the curvature is small, slight diffraction effects fail to prevent the transient detonation wave from developing. The propagation of the transverse detonation wave is affected not only by the diffraction effects but also by the mixture reactivity. When the curvature of the triple-point trajectory is large, a strong cylindrical shock wave is required to compress the mixture, enhancing its reactivity to ensure the transverse detonation wave can propagate without decoupling.
This paper presents a millimeter-wave end-fire dual-polarized (DP) array antenna with symmetrical radiation patterns and high isolation. The DP radiation element is formed by integrating a quasi-Yagi antenna (providing horizontal polarization) into a pyramidal horn antenna (providing vertical polarization), resulting in a DP radiation element with a symmetrical radiation aperture. To efficiently feed the DP element while maintaining high isolation, a mode-composite full-corporate-feed network is employed, comprising substrate-integrated waveguide supporting the TE10 mode and substrate-integrated coaxial line supporting the TEM mode. This design eliminates the need for additional transition structures, achieving excellent mode isolation and a reduced substrate layer number. A 1 × 4-element DP array prototype operating at 26.5–29.5 GHz using low temperature co-fired ceramic technology was designed, fabricated, and measured. The test results indicate that the prototype achieves an average gain exceeding 10 dBi for both polarizations within the operating band. Thanks to the symmetrical DP radiation element and mode-composite full-corporate-feed network, symmetrical radiation patterns for both polarizations are observed in both the horizontal and vertical planes, along with a high cross-polarization discrimination of 22 dB and polarization port isolation of 35 dB.
Hand, foot, and mouth disease (HFMD) shows spatiotemporal heterogeneity in China. A spatiotemporal filtering model was constructed and applied to HFMD data to explore the underlying spatiotemporal structure of the disease and determine the impact of different spatiotemporal weight matrices on the results. HFMD cases and covariate data in East China were collected between 2009 and 2015. The different spatiotemporal weight matrices formed by Rook, K-nearest neighbour (KNN; K = 1), distance, and second-order spatial weight matrices (SO-SWM) with first-order temporal weight matrices in contemporaneous and lagged forms were decomposed, and spatiotemporal filtering model was constructed by selecting eigenvectors according to MC and the AIC. We used MI, standard deviation of the regression coefficients, and five indices (AIC, BIC, DIC, R2, and MSE) to compare the spatiotemporal filtering model with a Bayesian spatiotemporal model. The eigenvectors effectively removed spatial correlation in the model residuals (Moran’s I < 0.2, p > 0.05). The Bayesian spatiotemporal model’s Rook weight matrix outperformed others. The spatiotemporal filtering model with SO-SWM was superior, as shown by lower AIC (92,029.60), BIC (92,681.20), and MSE (418,022.7) values, and higher R2 (0.56) value. All spatiotemporal contemporaneous structures outperformed the lagged structures. Additionally, eigenvector maps from the Rook and SO-SWM closely resembled incidence patterns of HFMD.