Haemonchus contortus is considered the most pathogenic gastrointestinal nematode of sheep and, due to the increasing resistance to synthetic anthelmintics, it poses a serious challenge to small ruminant production systems. The aim of this work was to evaluate the in vivo and in vitro anthelmintic activity of quebracho-chestnut tannin extract against Haemonchus contortus in lambs. The in vitro assays (egg hatching inhibition and larval migration inhibition) were performed to determine the effective concentrations required to inhibit 50% of egg hatching and larval migration (EC50), which were 3.8 and 1.4 mg/mL, respectively. For the in vivo trial, 18 lambs used were divided into three groups: Group 1 (control), Group 2 (a tannin dose equivalent to 0.3% of dry matter intake), and Group 3 (a tannin dose equivalent to 3% of dry matter intake). No significant differences (p > 0.05) were observed among treatments in faecal egg counts, larval counts from coprocultures, or adult H. contortus recovery. The tannin extract was effective in inhibiting egg hatching and larval migration in vitro at low concentrations. However, when administered in vivo at 0.3% and 3% of dry matter intake, it failed to show any effect on faecal egg counts, larval counts in coproculture, or adult worm burden. Adult worms, eggs, and infective larvae were further examined using scanning electron microscopy. The observations revealed significant cuticular alterations in adult worms, which could potentially interfere with normal feeding and reproductive processes. Additionally, eggs and infective larvae exhibited shell rupture and cuticle damage.

Ascidiella aspersa is a solitary ascidian native to the North-east Atlantic that has been introduced to many regions around the globe. In 2023, individuals matching the description of A. aspersa were found on an artificial dock structure in Stanley Harbour, Falkland Islands, where there were no previous records of the species. Individuals were collected for morphological and genetic analyses, and previous surveys of the site were reanalysed to estimate the abundance of the population. The morphological examination and genetic analysis confirmed the individuals were A. aspersa. Analysis of the survey data suggested the species has been present since at least 2011 and forms a reasonably dense population on the more sheltered areas of the dock structure. Further survey work and population genetic investigations are required to better understand the likely origin of the population, and the abundance and extent of the species around the Falkland Islands.

Cabbage root maggot, Delia radicum (Linnaeus) (Diptera: Anthomyiidae), has been an economically serious pest, damaging a wide range of Brassica crops, across Canada since the late 1800s. A robust body of research literature exists, encompassing a range of control options that have been explored across commodities within Canada. Despite this body of work, pesticides remain the most commonly used option for control of D. radicum. Insecticides registered for use against D. radicum in Canada are facing increasing restrictions or deregulation, making D. radicum more difficult to manage. This review provides an overview of the research conducted in Canada up to 2022 and discusses various management approaches that need to be explored to lessen our current reliance upon insecticides for D. radicum control.

Early-season crop yield loss frequently occurs even when resources are abundant, challenging traditional resource-based models of crop–weed competition. Drawing on decades of research on the critical period for weed control, this review highlights evidence that brief exposure of crop seedlings to neighboring weeds can trigger rapid and irreversible reductions in yield potential through resource-independent mechanisms. Central to these processes are weed-induced changes in light spectral quality, particularly reduced red:far-red (R:FR) ratios, which activate the phytochrome-mediated shade avoidance syndrome (SAS). These responses alter morphology, biomass allocation, canopy architecture, photosynthetic capacity, redox homeostasis, defense signaling, and nitrogen metabolism. Low R:FR light induces persistent photosynthetic and metabolic constraints, increases reactive oxygen species (ROS) signaling, suppresses jasmonic acid- and salicylic acid-mediated defenses, and modifies nitrate assimilation and root traits in species- and genotype-dependent manners. Collectively, weed-derived signals during early crop development can lead to lasting physiological reprogramming. Integrating light-mediated signaling with metabolic, defense, epigenetic, and lncRNA-mediated pathways provides a mechanistic framework for understanding yield loss and identifies potential targets for enhancing crop competitiveness and resilience in weed-infested agroecosystems.

Structural equation modeling (SEM) is a flexible statistical technique with multiple applications, including behavioral genetics and social sciences. Building on the original design of the umx package, which improved accessibility to OpenMx by specifying a concise syntax, umx v4.5 extends functionality for longitudinal and causal twin designs while improving interoperability with graphical modeling tools such as Onyx. New capabilities include: classic and modern cross-lagged panel models; Mendelian Randomization Direction-of-Causation (MR-DoC) twin models incorporating polygenic scores as instruments; support for definition variables directly in umxRAM(); a workflow for importing paths from Ωnyx; a dedicated function for incorporating censored variables’ data into models, particularly valuable in biomarker research; improved covariate placeholder handling for definition variables; sex-limitation modeling across five twin groups, accommodating quantitative and qualitative sex differences; and covariate residualization in wide- or long-format data. These new functionalities accelerate reproducible, reliable, publication-ready twin and family modeling, and integrated journal-quality reporting, thereby lowering barriers to genetic epidemiological analyses

Glufosinate-ammonium (GA) has been widely used in Midwest fields, and in recent years a growing number of failures to control waterhemp [Amaranthus tuberculatus (Moq.) Sauer] have raised concerns about the evolution of resistance. The goal of this study was to investigate four cases of suspected resistance to GA in A. tuberculatus from Illinois using greenhouse, field, and transcriptomics studies. Greenhouse dose-response experiments revealed resistance ratios ranging from 2.2- to 3.4-fold based on survival and 1.3- to 2.8-fold based on biomass relative to a susceptible population. A subsequent field study where one of the populations originated confirmed that twenty percent of treated plants survived the labeled GA field-recommended rate. Screening with other herbicide site-of-action groups revealed that most populations showed reduced sensitivity to atrazine, glyphosate, and imazethapyr, surviving up to three times the field-recommended rates, and to a lesser extent, lactofen and fomesafen. Transcriptomic analysis of plants surviving GA revealed no resistance-associated mutations or differential transcript abundance in the plastidic and cytosolic isoforms of glutamine synthetase. Among the four suspected resistant populations, there were 182 genes differentially expressed relative to two susceptible populations. Different sets of genes were differentially expressed among the populations studied, with only one gene (upregulated relative to two susceptible populations) shared among all four. Many of the differentially expressed genes, including cytochrome P450s, glutathione S-transferases, glycosyltransferases, transporters, and transcriptional regulators, are commonly associated with metabolic resistance. Gene ontology enrichment analyses indicated significant overrepresentation of stress response, defense regulation, and secondary metabolism categories across the populations. Together, these findings provide evidence for the evolution of GA resistance in populations of A. tuberculatus in Illinois. While more in-depth studies are needed to fully characterize the underlying mechanisms, the consistent differential expression of metabolism-related genes and no indication of target-site mechanisms points to a potential metabolic basis for resistance.

Within Public Health Nutrition, the preconception phase, has emerged as a particularly important window for promoting a healthy diet, as it affects both current and future health as well as health in the next generation. Different approaches are used to support dietary change in this phase, and digital interventions are viewed as particularly promising. In this review, we describe digital interventions addressing preconception diet, discuss challenges in targeting the preconception population, and highlight issues related to digital designs and measuring intervention effects. We draw upon six digital interventions with dietary outcomes, in different target populations. Only two of the interventions were found to be effective in improving relevant aspects of the participants’ diet, and these targeted specific and narrow groups or a single dietary component. Most of the intervention studies faced considerable challenges with recruitment. High attrition further complicated evaluation. In addition, there were difficulties related to participant engagement with the digital interventions. Challenges with lack of engagement, recruitment and attrition are not new insights within public health research, and we need to acknowledge the need for new approaches to recruitment, intervention development and evaluation. Alternative approaches such as citizen science and participatory action research in which the researchers work closely with the target population and other stakeholders during the whole process, should be explored. Moreover, as the current behavioural interventions that rely heavily on individual agency seem largely unsuccessful, future interventions should consider targeting more upstream and structural determinants of diet, rather than individual behaviour alone.

Giving animals the opportunity to exercise agency can improve their welfare, but horse owners and researchers may not be aware of the growing body of agency research in other animals, and studies on agency and choice in horses are scattered across disciplines and not connected to each other or to broader theory. This paper summarises research findings on management of domestic horses through the lens of animal agency and explores the potential applications of research on choice, control, and challenge in animals to improve the welfare of horses.

This Research Communication aimed to establish reference freezing point (FP) values for raw Bactrian and dromedary camel milk and to evaluate the detectability of water dilution up to 30% using the cryoscopy method. A total of 38 milk samples from healthy camels in Kazakhstan were analysed. Pure Bactrian camel milk exhibited FP values between −0.542°C and −0.799°C, while dromedary milk ranged from −0.527°C to −0.655°C. Progressive dilution caused a significant linear increase in FP in both species (p < 0.0001). Although a 10% water addition resulted in measurable changes, natural variability may mask slight adulteration. FP showed weak correlations with protein and pH, with species-specific differences. These results provide reliable reference values for the FP and confirm the possibility of detecting adulteration of camel milk.

Gestational weight gain (GWG) can be defined as the total weight gained throughout pregnancy and is required for healthy foetal growth, however gaining excessive weight during pregnancy has been linked with several adverse effects. This review aims to consider the evidence on weight management during pregnancy, with a focus on the key challenges surrounding GWG and the practical considerations related to assessing weight changes. It is estimated that nearly 50% of women gain excessive weight during pregnancy, nevertheless this can be difficult to quantify due to the lack of global consensus on recommended GWG guidelines. Currently there are no GWG guidelines in the UK and Ireland, as reiterated in the recent NICE guidelines, due to the lack of evidence about what the optimal total weight change in pregnancy should be. This is further complicated by the conflicting results of interventions aimed at preventing excessive GWG and their resultant inconsistent effects on adverse pregnancy outcomes. Accurate calculation of GWG requires measurement of pre-pregnancy weight and weight prior to the onset of labour. However, several practical considerations are associated with obtaining these weights, as in practice, estimated or self-recalled weights are often used as an alternate, thereby introducing variability into the measurement of GWG and the potential for inaccuracies in analysis. These limitations highlight the need for a more uniform approach in assessing GWG. The World Health Organisation are in the process of developing global GWG standards, this could potentially establish a uniform gold standard for assessing GWG and reintroduce routine weighing.

The Developmental Origins of Health and Disease (DOHaD) hypothesis highlights the pivotal role of early-life nutrition in shaping lifelong health and disease risk. Low birth weight (LBW) remains a major public health issue associated with increased susceptibility to metabolic and cardiovascular disease, underscoring the need for early nutritional interventions. We investigated whether dietary supplementation with soy protein isolate (SPI) during lactation could mitigate adverse developmental programming in a rat model of LBW induced by maternal calorie restriction. Dams received an SPI-supplemented diet during lactation, and offspring were evaluated for postnatal growth, circulating IGF-1 and corticosterone concentrations, and pituitary expression of Gas5 lncRNA, miR-23b, and Pomc. Stress responsiveness and glucocorticoid receptor sensitivity were also assessed. SPI supplementation restored postnatal growth and IGF-1 concentrations in female offspring, and in males, it normalized pituitary Gas5 lncRNA and Pomc mRNA expressions, reduced stress-induced corticosterone hypersecretion, and improved pituitary glucocorticoid sensitivity. These findings indicate that SPI intervention during lactation can partially reverse epigenetic dysregulation of the stress and somatotropic axes caused by fetal undernutrition. Nutritional modulation during lactation thus represents a critical window for early intervention in LBW offspring. SPI supplementation may enhance endocrine and metabolic resilience, providing a practical nutritional programming approach to reduce future disease risk, consistent with the DOHaD paradigm.

Smoking has been confirmed to induce systemic inflammation and oxidative stress (OS) and is associated with higher odds of chronic obstructive pulmonary disease (COPD). Dietary antioxidants can reduce inflammation and OS. This study seeks to score the dietary antioxidant intake and then assess its impact on the association between smoking and COPD in adults. The data extracted from the 2007-2012 NHANES database were used. The Dietary Antioxidant Quality Score (DAQS) was evaluated by the total intake of vitamins A, C, and E, selenium, zinc, and magnesium in the daily diet. Smoking was used as the exposure variable, and COPD as the outcome variable. Weighted multivariable logistic regression was conducted to evaluate the associations of DAQS with smoking and COPD, as well as their joint effects on the odds of COPD. The relationships between dietary antioxidant quality score, smoking status, and COPD were subsequently assessed. Subgroup analyses were performed to explore associations between relevant covariates and smoking and COPD across DAQS strata. Current smoking was found to be linked to COPD (OR=4.06, 95% CI=3.14-5.27) in comparison to never smoking. Among smokers, significant associations were observed in both the medium-quality DAQS group (OR =3.48, 95% CI: 2.34-5.17) and the low-quality DAQS group (OR = 5.60, 95% CI: 3.58-8.76). In conclusion, high DAQS levels are inversely related to the odds of COPD in adult smokers. Our findings provide valuable insights for management strategies for COPD.

Alligatorweed, an invasive aquatic weed, has emerged as a major threat to sustainable crop production in various crop species. A two-year field study was conducted to investigate the impact of varied competition durations of alligatorweed on mungbean. The competition durations with alligatorweed included weed free conditions for first 3, 4, 5, 6 and 7 weeks after crop emergence along with a full season weed free treatment and alternatively weedy conditions for the aforementioned durations along with a full season weedy treatment. Competition with alligatorweed led to significant uptake of nitrogen (N), phosphorus (P) and potassium (K), with maximum uptake observed in the full season weedy treatment with N, P and K up to 65, 19, 56 kg ha-1, respectively. Additionally, significant accumulation of heavy metals (HMs) including copper (Cu), iron (Fe), manganese (Mn), zinc (Zn) and arsenic (As) up to 20, 16, 30, 14 and 11 g ha-1, respectively, was observed. Full season weedy plots produced more alligatorweed biomass and caused reductions of up to 81% in mungbean yield components. Alligatorweed infestation resulted in significant mungbean grain yield losses of up to 44% during 2022 and 52% in 2023, respectively. Furthermore, the three-parameter log-logistic equations identified the period from 4.2 to 6.8 weeks after crop emergence (WACE) as the critical period of alligatorweed competition that could result in a 10% yield loss in mungbean. Hence, alligatorweed poses a significant threat to mungbean production due to its strong competitive ability. However, its potential for HM accumulation offers promising opportunities for phytoremediation in both aquatic and terrestrial environments.

The Bennettitales, a group of extinct gymnosperms, had outstanding species diversity throughout the Mesozoic and were abundant during the Jurassic. They were key components of terrestrial ecosystems, thriving in diverse climates and environmental conditions. However, a deeper understanding of the ecological strategies exhibited by this extinct lineage across time and space within a well-characterized geological context is still lacking for the region. Here, we examine bennettitalean assemblages from the Middle Jurassic Otlaltepec Formation (Otlaltepec Basin) and compare them with floras from two other rift basins (Tlaxiaco and Ayuquila) in low latitudes. Based on morphological features, we propose the new species Zamites ambigua, and identify the following additional taxa: Zamites lucerensis, Zamites oaxacensis, Zamites tribulosus, cf. Zamites diquiyui, Zamites sp. 1, Zamites sp. 2, Bennettitcarpus sp. 1, Bennettitcarpus sp. 2, and cf. Weltrichia xochitetlii, along with two types of gymnosperm foliage of uncertain affinities.

Our findings on bennettitalean diversity in the Otlaltepec Formation reveal the presence of foliage across all three basins in both time and space, indicating a generalist element among these rift environments. In contrast, other foliage and reproductive structures exhibit two distinct distributional patterns: some are confined to a single basin over time, while others appear only during specific intervals in each basin’s history. Our findings highlight that some Bennettitales exhibited ecological resilience, maintaining their morphology and distribution despite the shifting environmental conditions caused by tectonic activity. This suggests that certain generalist taxa persisted across varying moisture regimes and depositional settings, while others displayed more localized or temporally restricted distributions.

Drought is a critical issue for global agriculture making the development of drought-resilient crop varieties crucial. Sorghum bicolor (L.) Moench is a highly drought tolerant cereal crop with the potential to serve as a model for identifying drought-tolerant genes. Investigating drought-induced gene expression changes in S. bicolor can inform breeding strategies aimed at enhancing resilience in other crops in the Poaceae family. Our aim was to identify the genes and networks that are differentially expressed in drought stressed sorghum across multiple tissue types, not just leaves. Previously, we reported differences in phenotype in terms of biomass, photosynthetic traits and the concentration of specialized metabolites (dhurrin and phenolics) between well-watered and water-limited plants. Here, differential gene expression analysis was conducted for drought-stressed S. bicolor variety BTx623 using edgeR. Gene ontology enrichment analysis and Weighted Gene Correlation Network Analysis (WGCNA) were conducted to identify the over-represented functions of the differentially expressed genes and to identify clusters of genes that behave together as a response to drought, respectively. Gene expression changes were largely confined to the root (56 genes were found to be differentially expressed), with little differential expression in the leaves or sheaths and no significant differences in expression of key dhurrin pathway genes. Together, these results indicate that drought tolerance in the cultivated sorghum reference genotype BTx623 is associated primarily with root-specific transcriptional responses and provide a tissue-resolved baseline for future comparative analyses across sorghum genotypes and wild relatives differing in drought sensitivity and HCN potential.

The brazilwood tree Paubrasilia echinata is endemic to the Atlantic Forest of Brazil and is categorized as Endangered on the IUCN Red List as a result of habitat loss and overexploitation. Phylogenomic analysis has identified five groups based on genotype, including the arruda-RJ lineage endemic to the state of Rio de Janeiro. We propose a prioritization classification protocol for Atlantic Forest fragments and evaluate the effectiveness of conservation for this species in Rio de Janeiro. We collated a total of 164 occurrence records of P. echinata from fieldwork during 2004–2024 and from the Global Biodiversity Information Facility. We classified forest fragments containing the species as high, medium or low priority for conservation. The arruda-RJ lineage persists in 43 forest fragments in the coastal region, 30 of which are documented for the first time here. Most forest fragments are small and have an uneven population structure. Urbanization is the primary threat to the survival of this lineage. We documented protected areas in 33 fragments, and identified conservation opportunities. The greatest number of forest fragments is in the northern region where there are the most protected areas and the brazilwood populations hold the greatest local genetic diversity. We propose targeted conservation actions for P. echinata arruda-RJ in eight forest fragments identified as high priority and with the greatest potential for conservation of the lineage. We identified 25 fragments as medium priority and 10 fragments as low priority. Our approach is applicable to other lineages of P. echinata and is aligned with Global Biodiversity Framework targets.

Recurrent pregnancy loss (RPL) is a clinically important condition in women undergoing assisted reproductive technologies (ART). This study evaluated embryo morphology and morphokinetic parameters using time-lapse monitoring (TLM) and assessed embryo ploidy by preimplantation genetic testing for aneuploidy (PGT-A) in women with RPL compared with unexplained infertility (UEI) controls. A total of 190 patients (100 RPL, 90 UEI) and 1169 embryos (634 RPL, 535 UEI) cultured under TLM were analyzed. Clinical characteristics, embryo morphology, morphokinetics and ploidy status were compared between groups, and logistic regression was used to identify predictors of aneuploidy. The euploidy rate was significantly lower in the RPL group than in controls (43.5% vs. 52.3%, p = 0.018). Group-wise analysis of all embryos revealed differences in selected morphokinetic parameters (t9 and tSC; p < 0.05). However, morphokinetic timing of euploid embryos did not differ between groups, suggesting that accelerated development alone was not associated with chromosomal abnormality. Embryo morphology was the strongest predictor of aneuploidy in both cohorts. Notably, direct uneven cleavage was associated with a 3.4-fold increased risk of aneuploidy specifically in the RPL group. Although embryos from RPL patients showed relatively faster developmental kinetics, morphokinetic speed alone did not predict chromosomal competence. Instead, embryo quality remained the key determinant of aneuploidy, while the association between direct cleavage and aneuploidy highlights the potential clinical value of TLM when combined with PGT-A. These findings support a complementary role for TLM in embryo assessment in RPL patients.