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Entamoeba bovis is a common intestinal amoeba of ruminants, yet molecularly confirmed data from Europe remain limited. We investigated the occurrence, morphology and genetic variability of E. bovis in Italian domestic and wild ruminants and developed a rapid species-specific polymerase chain reaction (PCR) assay. Faecal samples from calves (n = 45), goats (n = 26), sheep (n = 11) and Italian red deer Cervus elaphus italicus (n = 25) were examined by sediment microscopy and iodine staining. Amoebic stages were detected in 57% of bovine, 61.5% of caprine, 100% of ovine and 56% of red deer samples. Across hosts, cysts were consistently uninucleate and displayed typical E. bovis morphology, including frequent chromatoid bodies and an iodophilic glycogen mass. Representative positives were sequenced at the 18S rRNA locus (∼800 bp), confirming E. bovis (98.6–99.8% identity; 100% query coverage). Maximum-likelihood phylogenetic analysis placed all Italian isolates within the E. bovis clade and indicated a shared lineage across domestic ruminants and red deer, with no segregation by host or ecological setting. A newly designed E. bovis-specific PCR (375 bp) consistently yielded a single amplicon in all sequenced positives, supporting its use for rapid screening without sequencing.
Attention-deficit/hyperactivity disorder (ADHD) involves altered neurodevelopment, yet the underlying mechanisms remain elusive. Aperiodic EEG components may reflect neural functions like excitatory/inhibitory balance, but their age-related differences in ADHD and link to default mode network (DMN) dysfunction are unexplored.
Methods
We included 110 medication-naïve children/adolescents with ADHD and 100 matched typically developing peers aged 6–14 years. Aperiodic parameters (exponent, offset) were derived, and source-localized alpha-band DMN coherence was computed. The sample was stratified into middle childhood (6–9 years) and early adolescence (10–14 years) subgroups to delineate age-dependent patterns.
Results
ADHD showed globally increased exponent and offset versus controls. Normative age-related decreases were significantly attenuated in ADHD, indicating divergence from typical development. Age-stratified analyses revealed distinct patterns: in middle childhood, increased frontal offset correlated positively with inattention (right hemisphere) and hyperactivity/impulsivity (left hemisphere); in early adolescence, it associated with reduced coherence in two DMN pathways (right mSFG–left hippocampus and left mSFG–right MTG).
Conclusions
Aperiodic activity differences in ADHD are age-dependent. Younger children exhibit focal, symptom-linked frontal abnormalities, whereas adolescents show pervasive network-level dysregulation. Aperiodic measures may capture age-varying ADHD pathophysiology, informing developmentally targeted biomarkers.
This study investigates how language learners prioritize criteria for evaluating data-driven learning (DDL) tools and how these priorities relate to tool preference. While previous research has proposed multiple criteria for evaluating the perceived effectiveness of DDL tools in capturing the emic dimension of the learner experience, empirical work has scantily examined how learners differentially prioritize these criteria, or how such priority structures can be empirically incorporated into systematic comparisons of tools. Given the recent emergence of generative artificial intelligence (GenAI) in language learning, this study examines how GenAI-based tools may contribute to DDL when judged against learner-valued effectiveness criteria. Drawing on a literature review, six criteria were identified: sentence comprehensibility, relevance to semantic learning, relevance to syntactic learning, perceived pedagogical value, accessibility for independent use, and support for autonomous learning. Thirty-five Korean EFL university students completed an analytic hierarchy process (AHP) task. First, they made pairwise comparisons among the six criteria, yielding priority weights that indicated the relative importance learners attach to each aspect of DDL effectiveness. Second, the study compared the perceived effectiveness of a traditional online concordancer that accesses the British National Corpus with that of a custom-developed GenAI-based concordancer, prioritizing these weighted criteria. The results indicate that learners assign differentiated importance to the six criteria and that these priority patterns are associated with the concordance they regard as more effective. This study demonstrates the usefulness of AHP for modelling multi-criteria, perception-based evaluations of DDL tools and incorporating learners’ priority judgements into the comparison of alternative tool designs.
Phonaesthetics examines why some languages are perceived as more aesthetically appealing than others, independent of meaning. Here we test whether phonetic and phonological properties predict listeners’ evaluations of 24 European languages using studio-quality recordings of native speakers reading the same text. 204 participants rated each recording on four dimensions: beauty, eros, status and order on 0–100 scales and indicated whether the language sounded familiar. Because familiarity reliably boosts evaluations, we first quantified its impact and then focused our main analyses on trials where languages were not recognized. We fitted Bayesian multilevel models with random intercepts for listener and language to examine a broad set of predictors: consonant place and manner distributions, vocalic share, voiced consonants, a sonority index, vowel height and backness and suprasegmental typology (speech rate, syllable structure, stress and rhythm type). Across most model families, effects were small and uncertain, with credible intervals (CrIs) overlapping zero, and variance was dominated by between-listener differences. The clearest and most consistent segmental signal was vowel height: a higher proportion of close vowels predicted lower status and order ratings. Overall, the results suggest that while a few fine-grained segmental cues may shape specific evaluative dimensions, phonaesthetic judgments are strongly shaped by listener-level variability, with only a small number of fixed individual-difference and phonetic predictors showing robust associations.
This research was conducted in response to a report by a producer in southwestern Ontario of severe corn injury following an application that included multiple herbicides, an activator adjuvant, and a drift reduction agent. Eight field trials were conducted between 2024 and 2025 near Ridgetown, Ontario, Canada, to evaluate corn tolerance to mixtures of multiple active ingredients, an adjuvant, and drift retardent applied at two early postemergence growth stages (V2 to V3 and V3 to V4). Isoxaflutole applied alone or in combination with atrazine at either growth stage caused minimal visible corn injury at all time points, with ≤1% injury at 1 wk after application (WAA) and no injury at 2, 4, 6, and 8 WAA. Isoxaflutole + atrazine + glyphosate, applied at the V2–V3 or V3–V4 stage, caused 7% and 9% visible corn injury at 1 WAA and 3% and 5% at 2 WAA, respectively; however, visible corn injury was ≤1% at 4, 6, and 8 WAA. Isoxaflutole + atrazine + glyphosate + HiActivate, applied at the V2–V3 and V3–V4 stages, caused greater and more persistent visible corn injury, with 7% and 16% injury at 1 WAA and 5% and 10% injury at 2 WAA, respectively; visible corn injury declined to ≤5% at 4, 6, and 8 WAA. Isoxaflutole + atrazine + glyphosate + HiActivate + InterLock, applied at the V2–V3 and V3–V4 stages, caused 17% and 28% visible corn injury at 1 WAA and 11% and 17% at 2 WAA, respectively. Visible corn injury persisted with up to 8%, 7%, and 4% injury at 4, 6, and 8 WAA, respectively. There was no adverse effect on corn height, biomass, and grain yield with any of the treatments evaluated at either application timing. Results indicate that tank mixtures with multiple active ingredients can increase visible corn injury, but the injury was transient and did not affect yield.
To evaluate the pathogen-specific diagnostic yield of rectal and groin screening sites in a hospital-based multidrug-resistant organism (MDRO) surveillance program.
Design:
Prospective observational surveillance study.
Setting:
Tertiary academic hospital in Northern Greece.
Patients:
Hospitalized patients undergoing active MDRO screening based on predefined infection prevention and control criteria.
Methods:
A pathogen-focused analysis was conducted using data from a prospective MDRO surveillance program between October 2024 and January 2025. Screening swabs were obtained from the rectum and groin. Target organisms included multidrug-resistant (MDR) Klebsiella pneumoniae, Pseudomonas aeruginosa, vancomycin-resistant enterococci (VRE), Candida auris, and Acinetobacter spp. Pathogen-specific detection yields were calculated for each anatomical site.
Results:
Among 1,206 screening swabs, 308 (25.5%) were positive for at least one MDRO. Rectal swabs detected 95.8% of Klebsiella pneumoniae (K. pneumoniae) (92/96), 100% of VRE (59/59), and 66.7% of Pseudomonas aeruginosa (46/69). In contrast, 88.9% of Candida auris cases (24/27) were identified exclusively from groin swabs. Reliance on rectal screening alone would have missed nearly 90% of Candida auris carriers, whereas groin-only screening would have failed to detect most K. pneumoniae and all VRE carriers.
Conclusions:
Anatomical site selection critically influences MDRO surveillance sensitivity. Rectal screening is optimal for MDROs colonizing the gastrointestinal tract, whereas groin sampling is essential for detecting Candida auris. Pathogen-targeted, site-specific surveillance strategies may substantially improve detection efficiency and optimize infection prevention efforts in high-prevalence hospital settings.
Gold mask burials constitute a remarkable cultural phenomenon linking diverse landscapes between the northern Aegean and the central Balkans. Their primary manifestation includes full-face masks and gold foils used to cover the body, accompanied by an ostentatious, largely standardized set of grave goods dating to the second half of the sixth century bce. The roots of this practice lie in earlier centuries, with modest gold sheets placed over the mouth. During the Late Archaic period, full masks and mouthpieces appeared in parallel, reflecting shared ritual traditions. This study traces the practice beyond the historical territory of the Macedonian kingdom, arguing against direct links to Macedonian ethnicity. By examining shared ceramic technology and settlement patterns, the paper suggests that the spread of this funerary custom was driven by medium- to large-scale mobility. This model favours fluid cultural interactions over the ‘heroic’ narratives of large-scale invasions or political expansions previously presumed to have occurred across these culturally diverse landscapes.
This paper studies a class of degenerate parabolic partial differential equation models that describe the dynamics of single-species populations with cognitive functions in toxic environments. The core innovation lies in introducing cognitive functions to simulate the ability of species to perceive toxins and adaptively adjust their behaviours, which in turn affects population density. This mechanism is characterized by a nonlinear degenerate Pratial Differential Equation. The degradation of the model is mainly manifested in the diffusion coefficient and response term approaching zero when the population density is zero or the cognitive state reaches the boundary. For this model, we have achieved the following theoretical breakthroughs: First, by using regularization techniques, we constructed a non-degenerate approximate system. Combining prior energy estimates with the fixed point theorem, we proved the existence of local classical solutions for this regularized system; subsequently, we constructed an appropriate Lyapunov function. Based on strict energy dissipation analysis and uniform prior estimates, we proved that under conditions such as bounded toxins and regular initial values, the classical solutions of the regularized system exist globally and remain bounded on the interval $[0, \infty )$ ultimately, based on this, through compactness theory and limit processes, the existence of the global weak solution for the original degenerate system is further derived. Numerical simulations verify the rationality of the theoretical results and visually demonstrate the dynamic regulatory effect of cognitive function on population tolerance.
Attention-deficit hyperactivity disorder (ADHD) is a common neurodevelopmental disorder that substantially affects behaviour, social functioning and daily performance. However, long-term population-based evidence on its burden in Jordan remains limited.
Aims
This study aimed to assess trends in incidence, prevalence and years lived with disability (YLDs) due to ADHD in Jordan from 1990 to 2023.
Method
A population-based descriptive analysis was conducted using data from the Global Burden of Disease (GBD) 2023 study. ADHD incidence, prevalence and YLDs in Jordan from 1990 to 2023 were extracted and stratified by gender and age group (2–9, 10–19 and 20–69 years). Estimates were reported as rates per 100 000 population, and absolute numbers with 95% uncertainty intervals, following standard GBD methods.
Results
ADHD burden was observed across all age groups in Jordan. Among children aged 2–9 years, incidence ranged from 279.9 to 352.3 per 100 000, prevalence exceeded 5000 per 100 000 in both 1990 and 2023 and YLD rates ranged from 214.8 to 262.8 per 100 000. In adolescents, the burden was concentrated among those aged 10–14 years, with prevalence around 526 per 100 000 and YLDs of 21 per 100 000 in both years; no estimates were reported for ages 15–19 years. In adults aged 20–69 years, although no incident cases were recorded, prevalence remained notable in 2023, ranging from 1626.8 per 100 000 (20–49 years) to 535.2 per 100 000 (50–69 years), with YLD rates between 69.1 and 22.4 per 100 000. Males consistently showed higher burden estimates than females across all age groups.
Conclusions
ADHD is a prevalent and a major source of non-fatal health loss in Jordan from early childhood through adulthood. These findings provide a comprehensive, long-term assessment of ADHD burden in Jordan and establish a robust evidence base for national, regional and global comparisons.
Election pledges are fundamental for representative democracy, linking parties’ campaigns before elections with their behavior afterwards. Yet little is known about voters’ awareness of these pledges before elections and how this affects post-election awareness of pledge fulfillment. Triangulating between a rich set of panel and pooled cross-sectional survey data surrounding the 2019 Danish national election (N = 10,322), we show that voters who were able to recognize and recall pledges before elections were more likely to be aware of their fulfillment status after the election. Furthermore, pre-election awareness mostly benefited people with less political interest, enabling these relatively disinterested voters to nevertheless hold parties accountable. These findings have important implications for our understanding of voters’ ability to make informed choices when they delegate power to parties at elections and hold them accountable afterwards.
Archaeology is in the midst of a technological revolution, particularly for data collection, first championed by light detection and ranging (lidar) in the early 2000s and now with widespread applications of unmanned aerial systems (UAS)-deployed remote sensing. While the importance of remote sensing for data collection and heritage conservation is known, less work has acknowledged the implications of remote sensing for field safety, particularly for disabled researchers. UAS offer a unique opportunity to explore circumstances that may affect mobility in the field and how advances in archaeological science can transform fieldwork by improving accessibility for disabled archaeologists. I argue that UAS can—and should—be improved to promote accessibility in archaeological fieldwork, due to their promotion of field safety, increased planning of daily tasks, and broader applications to community-engaged research. These interventions should be applied to the larger field of archaeology to improve labor management for researchers in all sectors of practice.
Reconstructions of Moche ritual sacrifice have relied heavily on iconographic depictions of elaborate rituals conducted on mountains and sacrifices at adobe temples. Scholars have categorized mountain scenes, proposed that Huaca de la Luna symbolized sacred mountains, and argued that one of the main mountain scenes entails the liberation of a figure known as Dios A. However, the large corpus of mountain iconography remains underanalyzed, and few existing publications incorporate data from mountainside sites. In this article, I propose a more specific Mountainside Sacrifice and Cave Opening mythic narrative encoded in these scenes, comparable to the Presentation Theme / Sacrifice Ceremony. I argue that future surveys and excavations must include thorough investigations of north coast mountains of Peru.
This work investigates projection-based reduced space–time models formulated in the frequency domain, employing space–time basis functions constructed with spectral proper orthogonal decomposition (SPOD) to represent dominant spatio-temporal structures. Although frequency-domain formulations are well suited to capturing time-periodic solutions, such as unstable periodic orbits, this study focuses on computing long-time approximate periodic solutions of statistically stationary flows whose statistics resemble those of the underlying chaotic attractor. In contrast to reduced-order models based solely on spatial modes, a space–time formulation achieves simultaneous reduction in both space and time via Galerkin projection of the Navier–Stokes equations onto the SPOD basis using a space–time inner product, yielding a quadratic algebraic system for the amplitude coefficients. Approximate solutions of the reduced system are obtained by identifying coefficients that minimise an objective function corresponding to the sum of squared residuals across all frequencies and modes, quantifying violation of momentum conservation within the reduced subspace. A robust gradient-based optimisation algorithm is used to identify minima. The method is demonstrated for chaotic flow in a two-dimensional lid-driven cavity at a Reynolds number equal to 20 000, where solutions with extended temporal periods approximately fifteen times the dominant shear-layer time scale are sought. Even without closure models to represent truncated spatio-temporal triadic interactions, multiple reduced-order solutions are found that identify approximate periodic orbits which reproduce dominant dynamical flow features and recover with good fidelity to the statistical distribution of the training data used to construct the basis, although these solutions tend to overpredict energy near the truncation boundary.