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Functional impairment in daily activities, such as work and socializing, is part of the diagnostic criteria for major depressive disorder and most anxiety disorders. Despite evidence that symptom severity and functional impairment are partially distinct, functional impairment is often overlooked. To assess whether functional impairment captures diagnostically relevant genetic liability beyond that of symptoms, we aimed to estimate the heritability of, and genetic correlations between, key measures of current depression symptoms, anxiety symptoms, and functional impairment.
Methods
In 17,130 individuals with lifetime depression or anxiety from the Genetic Links to Anxiety and Depression (GLAD) Study, we analyzed total scores from the Patient Health Questionnaire-9 (depression symptoms), Generalized Anxiety Disorder-7 (anxiety symptoms), and Work and Social Adjustment Scale (functional impairment). Genome-wide association analyses were performed with REGENIE. Heritability was estimated using GCTA-GREML and genetic correlations with bivariate-GREML.
Results
The phenotypic correlations were moderate across the three measures (Pearson’s r = 0.50–0.69). All three scales were found to be under low but significant genetic influence (single-nucleotide polymorphism-based heritability [h2SNP] = 0.11–0.19) with high genetic correlations between them (rg = 0.79–0.87).
Conclusions
Among individuals with lifetime depression or anxiety from the GLAD Study, the genetic variants that underlie symptom severity largely overlap with those influencing functional impairment. This suggests that self-reported functional impairment, while clinically relevant for diagnosis and treatment outcomes, does not reflect substantial additional genetic liability beyond that captured by symptom-based measures of depression or anxiety.
This work attempts to better understand the significance of morphological diversity among fungal-algal contact zones present in lichens. We used TEM to examine a variety of lichen symbioses involving non-trebouxialean green algae that show intraparietal penetration by the mycobiont. A principal focus was on Endocarpon pusillum, a well-known member of a family (Verrucariaceae; Eurotiomycetes) previously reported to be characterized by unwalled haustoria exposing a naked fungal protoplast. Peg-like haustoria arose from an inner layer(s) of the mycobiont cell wall that broke through outer layers and penetrated a short distance into the wall of the green algal symbiont (Diplosphaera). In both fungal and algal cells at the contact interface, lomasome-like vesicles and tubules occurred as modifications of the plasmalemma intermixed with wall materials at the inner surface of the cell wall. A fungal cell wall was consistently present around the haustorium, which resembled those depicted in earlier TEM studies of Verrucariaceae. Previously published micrographs of Verrucariaceae purporting to show wall-less haustoria surrounded by an empty space are believed to have been misinterpreted. However, in the isidiose Porina and foliicolous Calopadia, Byssoloma and Fellhanera species (Lecanoromycetes), we did observe extreme degrees of reduction in the mycobiont cell wall at symbiont contact interfaces. In those lichens, a broad area of the fungal cell bulged into the adjacent algal symbiont, broadly invaginating the wall of the latter and penetrating it intraparietally without differentiation of a distinct haustorial structure. The mycobiont wall surrounding such protrusions often thinned to near indistinguishability towards its extremity. The protrusion made direct contact with the algal cell wall; no empty space occurred between them. We propose that the short, peg-like intraparietal haustoria bind the symbionts and help maintain cell contacts amid the stresses of tissue expansion and shrinkage, thereby avoiding disruption of the continuous hydrophobic coating that facilitates transfer between them. Broader contact interfaces with extremely thin adjacent walls may facilitate solute flow between symbionts. Reciprocal penetration of algal protrusions into mycobiont cells, noted in Porina as well as other lichens studied previously, is a neglected but potentially significant indication that both symbionts may actively work to maintain functional contact interfaces.
This study sought to examine the validity and reliability of the Turkish adaptation of the Peace, Equanimity, and Acceptance in the Cancer Experience (PEACE) scale. The primary objective was to evaluate the scale’s psychometric properties in measuring acceptance and coping among cancer patients.
Methods
The study included 90 cancer patients who completed the 12-item PEACE scale. The scale consists of two distinct subscales: the 5-item Peaceful Acceptance subscale and the 7-item Struggle With Illness subscale. Reliability was examined using Cronbach’s alpha and test-retest reliability (r = 0.916). Content validity was assessed using the content validity index (CVI = 0.84). Both exploratory factor analysis (EFA) and confirmatory factor analysis (CFA) were employed to examine the underlying factor structure and evaluate model fit indices.
Results
The internal consistency for both subscales was satisfactory (Cronbach’s α = .78 for both). EFA indicated that the two subscales explained 53.169% of the total variance. CFA substantiated the two-factor model, demonstrating adequate model fit indices (χ2/df = 1.689,Root Mean Square Error of Approximation = 0.088). These findings collectively establish the Turkish version of the PEACE scale as a psychometrically sound tool.
Significance of Results
The PEACE scale is a valid and reliable instrument for assessing levels of acceptance and coping in cancer patients. Its use can help healthcare professionals better understand patients’ emotional states and guide interventions aimed at improving their quality of life.
Adolescents may not necessarily have a specific mental health challenge to seek information on mental health. They may be genuinely curious on how to better understand these issues, especially when mental health is being discussed in school, among peers and with parents. The purpose of this study was to examine the frequency and factors associated with online information seeking about mental health among adolescents. A total of 702 high school students from Belgrade, Serbia, participated in the study and filled in an anonymous questionnaire about sociodemographics, digital behaviors and the Electronic Health Literacy Scale (eHEALS). The prevalence of seeking information about mental health in our study sample was 23.5% (165/702). The multivariate model showed that having a lower school performance, lower eHEALS score and browsing health blogs, social media and websites run by physicians and health institutions were independently associated with online information seeking about mental health. Additionally, searching for online information about psychoactive substances, bullying and medications was independently associated with online information seeking about mental health among adolescents. Adolescents are familiar with a variety of sources of online health information, but choose specific online platforms to read about mental health. These platforms could be utilized to promote mental well-being in high schools.
To evaluate the impact of a two-week otolaryngology rotation incorporating entrustable professional activities, human factors and simulation on medical students’ knowledge, perceptions and career aspirations.
Methods
The curriculum included six small-group sessions on compassion, communication, resilience, teamwork and professionalism, and three simulations: suturing, flexible nasendoscopy and grommet insertion. These were delivered alongside standard teaching. Pre- and post-rotation questionnaires assessed otolaryngology knowledge, career interest, surgical confidence and attitudes toward simulation and human factors.
Results
While students’ interest in surgical careers remained unchanged, they reported improved comfort with otolaryngology knowledge, operating theatre environments and recognition of non-technical skills. Perceptions of simulation and essential surgeon qualities significantly improved.
Conclusion
Integrating entrustable professional activities, human factors education and simulation into short surgical rotations enhances both technical and non-technical skills. This approach may help address challenges in attracting students to surgery by enriching their educational experience and building confidence.
This paper develops methods for simplifying systems of partial differential equations (PDEs) that have families of conservation laws which depend on arbitrary functions of the independent or dependent variables. Cases are identified in which such methods can be combined with reduction using families of symmetries to give a multiple reduction; this is analogous to the double reduction of order for ordinary differential equations (ODE) with variational symmetries. Applications are given, including a widely used class of pseudoparabolic equations and several mean curvature equations.
Anapachydiscus (Anapachydiscus) haegerti n. sp., an ammonite belonging to the Pachydiscidae, is reported for the first time from the upper Campanian Northumberland Formation of Hornby Island, British Columbia. Thirty-five specimens have allowed for the complete ontogenetic reconstruction of the taxon as well as thanatocoenosic (death assemblage) observations with paleoecological implications for ammonoid early life. Although isolated juvenile specimens occur, instances of concretions containing a multitude of individuals are typical. One concretion yielding twenty-six early-stage juveniles lends support to a mode of preservation reflective of life association through a taphonomic process of capture and burial. The new species presents age-diagnostic utility as a distinct fossil within the molluscan assemblage of the Nostoceras (Didymoceras?) adrotans regional subzone; a highly constrained interval of the upper Campanian in the eastern North Pacific. A specimen attributed to Anapachydiscus (Anapachydiscus) cf. A. (A.) fascicostatus from the upper Campanian of the Cedar District Formation on Denman Island is also described, and the genus Anapachydiscus is retained with an emended diagnosis. Additional collections have enabled revised conceptualizations of Pachydiscus (Pachydiscus) hornbyense and Pachydiscus (Pachydiscus) ootacodensis accounting for the spectrum of variance within these species. Recognition of an upper Campanian P. (P.) ootacodensis–Pachydiscus (Pachydiscus) suciaensis Concurrent-range Zone is proposed for the eastern North Pacific to assist broader efforts of interregional biostratigraphic correlation given the endemic limitation of the latter taxon.
Squamate faunas from the lower Eocene of Europe are rare. We here describe pleurodontan iguanian (potentially Geiseltaliellus Kuhn, 1944), scincoid, and Squamata indet. jaw remains from Cos locality (near the Caylus village, southwestern France). The age of the Cos deposit has been proposed to fit the MP 10−11 interval (MP 10b; late Ypresian). Thus, it either corresponds to the end of the Early Eocene Climatic Optimum (EECO) or slightly postdates it. Although very fragmentary, the finds represent the first evidence of these clades in this locality, which is one of the oldest from Phosphorites du Quercy, adding to the squamate paleobiodiversity of the site. Besides iguanians and scincoids, the fauna also includes gekkotans, glyptosaurids, varanoids, and a constrictor snake. Some of the genera from Cos are known solely from this locality, revealing a crucial part of the squamate history in Europe. In the last few years, our knowledge of the Paleocene and especially early Eocene lizard faunas has increased. This allows a better understanding of the faunas and their changes due to temperature changes and migrations. Records are still very sketchy at European localities, but the overall picture is somewhat clearer, even on a smaller scale. A small but visible drop in lizard diversity appears to be present at localities from MP 8−9 relative to those from MP 7, whereas at MP 10, the diversity slightly increased. This appears to correlate well with observed changes in temperature.
Measurement-based care (MBC) is widely recommended in psychiatry but remains underutilized in routine clinical settings. The Transdiagnostic Global Impression – Psychopathology (TGI-P) scale was developed to provide a brief yet comprehensive assessment of 10 core transdiagnostic symptom domains. To support more inclusive care and promote patient and caregiver engagement in treatment planning, two new versions of the TGI-P, that is, a patient-rated and a separate informant-rated, were developed, complementing the previously published clinician-rated version.
Methods
The patient and informant versions mirror the original clinician-rated TGI-P, assessing the identical 10 domains using a seven-point Likert severity scale, with results displayed via a personalized symptom map. A user satisfaction/feasibility study was conducted with 50 participants (25 patients and 25 caregivers) from the UK and US. After completing the scale, participants provided feedback on its clarity, usability, emotional impact, and comparative utility.
Results
Most participants completed the scale in less than 5 min. Instructions were considered clear, and the format was rated easy to follow. Response options were deemed appropriate by 86% of participants, and the visual output was widely appreciated. While one-third reported mild emotional triggering, overall burden was described as manageable. Approximately, three-quarters of participants rated the TGI-P as equal to or better than other tools they had used.
Conclusions
TGI-P patient and informant versions were developed and, informed by the feasibility study, refined to offer brief, user-friendly tools that support multi-informant assessment as input to MBC. Both versions of the TGI-P, with their graphical output, may support shared understanding and collaborative decision making among clinicians, patients, and caregivers. A validation study of the TGI-P is underway.
A new species of syngnathiform fish, Gerpegezhus daniaoriundus n. sp., from the Eocene Fur Formation of Denmark is described herein. The description is based on 17 specimens preserved in either soft diatomite or carbonate concretions. The two lithologies result in different preservation of the morphological features. Gerpegezhus daniaoriundus n. sp. exhibits a set of diagnostic features of the extinct monotypic family Gerpegezhidae and of the genus Gerpegezhus, including (1) greatly elongated body, (2) presence of ossified myoseptal tendons, (3) lower procurrent caudal-fin rays absent, (4) dorsal- and anal-fin spines absent, and (5) pelvic fin and girdle absent. It can be separated from the species Gerpegezhus paviai by having a much slenderer body bearing unpaired leaf-like appendages protruding from its ventral side, and completely different meristic values, including up to 39 (or 40) vertebrae, a total of 16 unbranched caudal-fin rays, dorsal and anal fins with 5 and 16 unbranched rays, respectively, and a different organization of body armor comprising two dorsal bilateral series of dermal plates. The occurrence of the genus Gerpegezhus from the Fur Formation provides a remarkable example of the biogeographic relationships between the North Sea realm and the Tethys in the earliest Eocene.
We study the behaviour of (resonant) dynamic B-tipping in a forced two-dimensional nonautonomous system, close to a nonsmooth saddle-focus (NSF) bifurcation. The NSF arises when a saddle-point and a focus meet at a border collision bifurcation. The emphasis is on the Stommel 2-box model, which is a piecewise-smooth continuous dynamical system, modelling thermohaline circulation. This model exhibits an NSF as parameters vary. By using techniques from the theory of nonsmooth dynamical systems, we are able to provide precise estimates for the general tipping behaviour close to the bifurcation as parameters vary. In particular, we consider the combination of both slow drift and also periodic changes in the parameters, corresponding, for example, to the effects of slow climate change and seasonal variations. The results are significantly different from the usual B-tipping point estimates close to a saddle-node bifurcation. In particular, we see a more rapid rate of tipping in the slow drift case, and an advancing of the tipping point under periodic changes. The latter is made much more pronounced when the periodic variation resonates with the natural frequency of the focus, leading both to much more complicated behaviour close to tipping and also significantly advanced tipping in this case.
This study aimed to adapt and validate a Food Retail Environment Analysis Protocol in Shiraz, Iran.
Design:
The protocol was developed by integrating the Nutrition Environment Measurement Survey in Stores with the food retail module from the International Network for Food and Obesity/Non-communicable Diseases Research, Monitoring, and Action Support. After translating, synthesising and back-translating the protocol, a panel of experts reviewed and refined it to ensure cultural and contextual appropriateness. Its validity was assessed through expert evaluation, and the pre-final version was field-tested to assess reliability across different food retail environments.
Setting:
Shiraz City, a metropolis in Iran
Participants:
Nine food retail stores, including kiosks, small- and medium-sized food retailers (comparable to convenience stores) and large food retailers (comparable to grocery stores).
Results:
Content and face validity were assessed using the content validity ratio (0·64–1), content validity index (0·78–1) and item impact score (2·84–4·83). Reliability testing by two researchers showed a 93·77 % agreement and an intraclass correlation coefficient of 0·89–1. The protocol includes fourteen food groups, most of which are categorised as either healthy or unhealthy. It evaluates product availability, prominence, quality, pricing and both in-store and out-of-store food promotions.
Conclusion:
The validated protocol effectively assesses diverse retail food environments, offering essential data for evaluating policies and guiding interventions to improve healthy food access. It is adaptable for broader regional or international application in public health and food policy contexts.
Recently, the Kac formula for the conditional expectation of the first recurrence time of a conditionally ergodic conditional expectation preserving system was established in the measure-free setting of vector lattices (Riesz spaces). We now give a formulation of the Kakutani–Rokhlin decomposition for conditionally ergodic systems in terms of components of weak order units in a vector lattice. In addition, we prove that every aperiodic conditional expectation preserving system can be approximated by a periodic system.
We prove almost global well-posedness for quasilinear strongly coupled wave-Klein-Gordon systems with small and localized data in two space dimensions. We assume only mild decay on the data at infinity as well as minimal regularity. We systematically investigate all the possible quadratic null form type quasilinear strong coupling nonlinearities.
A key feature of the paper is our new, robust approach to the vector field method, which enables us to work at minimal regularity and decay in a quasilinear setting, and which, we believe, can be applied for a much wider class of problems.
An analytical expression for focal intensity is derived for arbitrary surface profiles and arbitrary groove patterns of compressor gratings. The expression is valid for different compressor designs: plane and out-of-plane compressors, symmetric and asymmetric compressors (compressors composed by two not-identical pairs of gratings) and a two-grating compressor. It is shown that the quality requirements for the optics used to write a grating are higher than for the grating. The focal intensity can be maximized by rotating each grating around its normal by 180 degrees. Moreover, it may be increased to maximum by interchanging any two gratings in the compressor, because imperfections of an individual grating do not additively affect the focal intensity. The intensity decrease is proportional to the squared pulse spectrum width and the squared total distortions of the second and third gratings of the four-grating compressor and the total distortions of two gratings of the two-grating compressor.
Rivers act as long-term plastic storage and a pathway for land-based plastic pollution into the ocean. Monitoring river plastic at a global scale remains challenging, with only limited large-scale and long-term monitoring efforts to date. Citizen science approaches may ensure a more continuous basic knowledge of plastic pollution in rivers, which can be used to assess the efficacy of reduction measures. We evaluated the suitability of several river plastic monitoring methods for citizen science, through field monitoring and a subsequent survey with citizen scientists in Accra, Ghana. Four measurement techniques (visual counting, macroplastic net sampling, microplastic net sampling and hydrometric measurements) were tested in the field and evaluated by citizen scientists. The visual counting method, used to estimate floating macroplastic transport, emerged as the most promising method for citizen science–based river plastic monitoring. Using the data collected by citizens, we quantify the variability in transport and concentration of both macroplastic and microplastic.
College students gain a considerable amount of weight by consuming unhealthy food. Many universities adopt costly programs to alleviate this problem. We study the effect of a simple, inexpensive option: moving unhealthy items out of sight. The opportunity to investigate this intervention comes from the decision of a dining hall in the University of New Hampshire that relocated cookies from a main section in plain sight to an out-of-the way corner. The cost of cookies did not change, since the dining hall operates as an “all that you can eat” restaurant. Relative to pizza, a product that did not change location, the consumption of cookies dropped by up to 22% relative to their predicted level had the relocation not taken place. We see this as evidence that simple changes in design can nudge students towards healthy eating.
The NewTools project aims to support the transformation of the food system by developing summary scores for the nutritional value and environmental and social sustainability of foods and exploring potential applications. In this conceptual paper, we present the governance, objectives, conceptualisation and expected outcomes of the NewTools project.
Design:
A cross-sector research partnership involving actors across the Norwegian food system.
Setting:
The need to transform food systems both globally, regionally and nationally.
Participants:
A broad constellation of twenty-eight project partners includes research institutions, governmental agencies, food industry and Non-governmental organization (NGO).
Expected results:
Outputs from the project will include the development and testing of a score for nutritional quality using the European Nutri-Score version 2023 as a starting point, identifying of indicators to measure social and environmental sustainability, proposing weighting of these into one or several summary scores, pilots testing potential applications of use for the scores and protocols for relevant spin-off projects.
Conclusion:
The multitude of perspectives represented by this unique variety of partners is seen as valuable to better understand the opportunities and limitations of the proposed tools designed to foster transformations towards a more resilient and sustainable food system.