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Elevated levels of IL-6 in plasma are associated with the severity of visceral leishmaniasis (VL). The clinical manifestations of VL vary among patients, influenced by host factors and the virulence of the Leishmania infantum parasite. Considering that severe VL may result from an exaggerated inflammatory response, this study investigated whether IL-6 could serve as a biomarker to identify pro-inflammatory virulence factors. We conducted a genome-wide association study (GWAS) analysis on L. infantum isolates from patients with VL, whose IL-6 concentrations were measured. The analysis revealed that the relationship between IL-6 levels and clinical outcomes (survival vs mortality) had an area under the curve (AUC) of 0.67 (95% CI 0.52–0.81). A cut-off of 391.7 pg mL−1 for IL-6 was established to conduct a logistic regression analysis. We identified 10 029 single nucleotide variants (SNVs) across 62 genomes, resulting in 6,948 SNVs after filtering, of which 6,341 are located in protein-coding regions. The association analysis with PLINK identified 722 variants, of which 35 showed significant associations, with odds ratios ≥3.3, primarily in coding regions. These findings demonstrate that IL-6 levels tended to be associated with the fatal outcome of VL and highlight 35 novel genetic variants that could serve as potential biomarkers for prognosis. Further research into the biological role of these variants may lead to new therapeutic targets and improve the clinical management of VL, especially in identifying high-risk patients.
There is limited information on the crop safety and weed control potential of commercially available PRE herbicides when applied under plastic mulch on various cucumber and summer squash cultivars grown in Florida. Two cucumber field trials were conducted at the Gulf Coast Research and Education Center in Wimauma, FL, in fall 2021 and spring 2022 to determine the effects of halosulfuron, pendimethalin, S-metolachlor, sulfentrazone, fomesafen, napropamide, oxyfluorfen, and flumioxazin on crop growth and development, yield, and the control of various weed species in the fields. We conducted this trial using three cucumber cultivars: ‘Speedway’, ‘Dominator’, and ‘Mongoose’. Two summer squash field trials were conducted simultaneously, evaluating all the mentioned herbicides, except flumioxazin, in addition to rimsulfuron on three summer squash cultivars: ‘Spineless Beauty’, ‘Payload’, and ‘Everglade’. In the cucumber trials, crop damage varied with cultivar and ranged from 3% to 16% in fall 2021. All herbicides caused ≥10% crop injury, except oxyfluorfen and flumioxazin, at 28 d after transplanting (DATr) in spring 2022. In the summer squash trial, halosulfuron, S-metolachor, and flumioxazin were the three most injurious PRE herbicides, causing more than 10% crop injury in seed-grown summer squash, with no effect of PRE herbicides on crop injury in transplant-grown summer squash in fall 2021. In spring 2022, crop injury with PRE herbicides varied with cultivar, where pendimethalin and S-metolachlor were consistently the most injurious PRE herbicides, causing 14% to 25% injury at 28 DATr. All PRE herbicides caused some damage to cucumber and summer squash, with limited differences between cultivars and no effect on overall crop yield.
The ICD-11 introduced a new diagnosis of complex post-traumatic stress disorder (CPTSD) defined by disturbances in self-organisation in addition to traditional post-traumatic stress disorder symptoms. The International Trauma Questionnaire (ITQ) is the established measure of this construct and has been validated for use in a variety of populations and languages; however, evidence for the measure's use in Latin America is limited.
Aims
This study sought to validate the factor structure of the Latin American Spanish version of the ITQ in a trauma-exposed sample in Colombia.
Method
Confirmatory factor analysis was used to assess a range of factor models validated previously, including first- and second-order factor models.
Results
Assessment of fit indices demonstrated that a correlated six-factor model comprised of re-experiencing, avoidance, sense of threat, affect dysregulation, negative self-concept and disturbed relationships provided the best fit for these data. Factor loadings for this model were found to be high and statistically significant.
Conclusion
Results concur with prior research validating the use of alternative language versions of the ITQ internationally, and with the theoretical underpinnings of the CPTSD diagnostic category. The ITQ is therefore a valid measure of CPTSD in this Latin American sample. Further validation research is needed in clinical populations in this region.
In our digitalized modern society where cyber-physical systems and internet-of-things (IoT) devices are increasingly commonplace, it is paramount that we are able to assure the cybersecurity of the systems that we rely on. As a fundamental policy, we join the advocates of multilayered cybersecurity measures, where resilience is built into IoT systems by relying on multiple defensive techniques. While existing legislation such as the General Data Protection Regulation (GDPR) also takes this stance, the technical implementation of these measures is left open. This invites research into the landscape of multilayered defensive measures, and within this problem space, we focus on two defensive measures: obfuscation and diversification. In this study, through a literature review, we situate these measures within the broader IoT cybersecurity landscape and show how they operate with other security measures built on the network and within IoT devices themselves. Our findings highlight that obfuscation and diversification show promise in contributing to a cost-effective robust cybersecurity ecosystem in today’s diverse cyber threat landscape.
In the astrophysics community it is common practice to model collisionless dust, entrained in a gas flow, as a pressureless fluid. However, a pressureless fluid is fundamentally different from a collisionless fluid – the latter of which generically possess a non-zero anisotropic pressure or stress tensor. In this paper we derive a fluid model for collisionless dust, entrained in a turbulent gas, starting from the equations describing the motion of individual dust grains. We adopt a covariant formulation of our model to allow for the geometry and coordinate systems prevalent in astrophysics, and provide a closure valid for the accretion disc context. We show that the continuum mechanics properties of a dust fluid corresponds to a higher-dimensional anisotropic Maxwell fluid, after the extra dimensions are averaged out, with a dynamically important rheological stress tensor. This higher-dimensional treatment has the advantage of keeping the dust velocity and velocity of the fluid seen, and their respective moments, on the same footing. This results in a simplification of the constitutive relation describing the evolution of the dust rheological stress.
We investigated the hypotheses that broilers and pigs have distinct starch digestion capacities and that different cereals could trigger diet–species interactions. Ten replicates of two broilers (14 d old) or one pig (50 d old) each were distributed into a 3 × 2 randomised factorial design with three pelleted diets (maize, barley or oat-based) and the two species. Nutritional composition was equal for both species. Diets were fed for 10 d, and then the pancreas and organs from the stomach region and small intestine were collected with contents. It was observed that both species were similarly efficient at digesting starch but differed in some digestive aspects. Broilers had higher ileal digestibility coefficients (P < 0·001) of DM (0·69) and crude protein (0·75) than pigs (0·66 and 0·67), presented a higher volume of particles < 0·1 mm in duodenal digesta (P < 0·001) and had a lower gizzard pH (3·68) than pig stomach (4·48; P < 0·05). Conversely, pigs had lower ileal viscosity (1·44 v. 2·77 cP; P < 0·05) and higher pancreatic lipase activity (27 v. 5·9 U/g of pancreas; P < 0·05) compared with broilers. In the jejunum, oat led to higher starch digestibility (0·96; P < 0·05) than maize and barley regardless of species. In the ileum, starch digestibility was higher for broilers fed oats (0·99) than broilers fed barley (0·94; P < 0·05), establishing that oats provided, in general, a superior starch availability. The results imply that starch utilisation capacity is more related to its dietary source than to the species to which it is fed.
Working memory encompasses the limited incoming information that can be held in mind for cognitive processing. To date, we have little information on the effects of bilingualism on working memory because, absent evidence, working memory tasks cannot be assumed to measure the same constructs across language groups. To garner evidence regarding the measurement equivalence in Spanish and English, we examined second-grade children with typical development, including 80 bilingual Spanish–English speakers and 167 monolingual English speakers in the United States, using a test battery for which structural equation models have been tested – the Comprehensive Assessment Battery for Children – Working Memory (CABC-WM). Results established measurement invariance across groups up to the level of scalar invariance.
Nearly 25% of people with intellectual disability (PwID) have epilepsy compared to 1% of the UK general population. PwID are commonly excluded from research, eventually affecting their care. Understanding seizures in PwID is particularly challenging because of reliance on subjective external observation and poor objective validation. Remote electroencephalography (EEG) monitoring could capture objective data, but particular challenges and implementation strategies for this population need to be understood.
Aim
This co-production aimed to explore the accessibility and potential impact of a remote, long-term EEG tool (UnEEG 24/7 SubQ) for PwID and epilepsy.
Method
We conducted six, 2-hour long workshops; three with people with mild intellectual disability and three with families/carers of people with moderate–profound intellectual disability. Brief presentations, easy read information and model demonstrations were used to explain the problem and device. A semi-structured guide developed by a communication specialist and art-based techniques facilitated discussion with PwID. For family/carers, active listening was employed. All conversations were recorded and transcribed. Artificial intelligence-based coding and thematic analysis (ATLAS.ti and ChatGPT) were synthesised with manual theming to generate insights.
Results
Co-production included four PwID, five family members and seven care professionals. Three main themes were identified: (1) perceived benefits for improving seizure understanding, informing care and reducing family and carer responsibility to accurately identify seizures; (2) the device was feasible for some PwID but not all; and (3) appropriate person-centred communication is essential for all stakeholders to reduce concerns.
Conclusions
The workshops identified key benefits and implementing barriers to SubQ in PwID.
The Earth magnetopause, when sufficiently plane and stationary at a local scale, can be considered as a ‘quasi-tangential’ discontinuity, since the normal component of the magnetic field $B_n$ is typically very small but not zero. Contrary to observations, the ‘classic theory of discontinuities’ predicts that rotational and compressional jumps should be mutually exclusive in the general case $B_n \ne 0$, but allows only one exception: the tangential discontinuity provided that $B_n$ is strictly zero. Here we show that finite Larmor radius (FLR) effects play an important role in the quasi-tangential case, whenever the ion Larmor radius is not fully negligible with respect to the magnetopause thickness. By including FLR effects, the results suggest that a rotational discontinuity undergoes a change comparable to the change of a shear Alfvén into a kinetic Alfvén wave when considering linear modes. For this new kind of discontinuity, the co-existence of rotational and compressional variations at the magnetopause does no more imply that this boundary is a strict tangential discontinuity, even in one-dimensional (1-D)-like regions far from X lines if any. This result may lead to important consequences concerning the oldest and most basic questions of magnetospheric physics: how can the magnetopause be open, where and when? While the role of FLR is established theoretically, in this paper we show that it can be proved experimentally. For this, we make use of magnetospheric multiscale mission (MMS) data and process them with the most recent available four spacecraft tools. First, we present the different processing techniques that we use to estimate spatial derivatives, such as $grad(B)$ and $div(P)$, and the magnetopause normal direction. We point out why this normal direction must be determined with extremely high accuracy to make the conclusions unambiguous. Then, the results obtained by these techniques are presented in a detailed case study and on a statistical basis.
Diet indices are quantitative assessments of the quality of population intake. Understanding diet quality is crucial to support health and well-being; however, knowledge of diet quality across racial groups is limited. To examine diet quality of acial groups ‘White’, ‘Black’, ‘Asian’, and ‘Other’ in the United Kingdom (U.K.) and United States (U.S.), U.K. and U.S. national survey data were used to calculate Alternative Healthy Eating Index (AHEI-2010), Diet Quality Index-International (DQI-I), and EAT-Lancet scores. ANCOVA tests compared median total quality scores across racial groups adjusting for covariates. Kruskal–Wallis tests examined differences in individual component scores. Spearman correlations identified association of diet quality scores across indices. Highest diet quality scores were reported for U.K. and U.S. Asian groups. Most noticeable differences were apparent between U.S. Asian and White/Black groups (62% Asians within highest tertile of AHEI-2010 score vs. 29% Whites; P < 0.001). All racial groups demonstrated poor diet quality in terms of sustainability; EAT-Lancet scores were <40% of maximum total score for U.S. White, Black, and Other groups. AHEI-2010 diet quality scores were moderately associated with EAT-Lancet scores, evident across all groups (r = 0.53–0.65; P < 0.001). There is a need for all groups to increase intake of wholegrains, especially Black groups (mean Wholegrain score for U.S. Black group within DQI-I was 0.60 (maximum score of 5)) as demonstrated within AHEI-2010, DQI-I, and EAT-Lancet component scores. Additionally, increased intake of vegetables and legumes and decreased intake of processed and red meat would improve the adequacy, healthiness, and sustainability of U.K. and U.S. racial diets.
Under what conditions are people more likely to support judicial invalidation of legislative acts? We theorize that constitutional recency confers greater democratic legitimacy on constitutional provisions, reducing concerns that judges may use dated language to impose their own will on a living majority. Exploiting differences among US state constitutions, we show in a pre-registered vignette experiment and conjoint analysis that Americans are more supportive of judicial review and original intent interpretation when presented with a younger constitutional provision or constitution. These results imply that Americans might alter their approach to the US Constitution if it were changed as easily and as often as a typical state constitution.
Cestodiases, like echinococcoses and cysticercoses, represent a global health problem. Currently available anthelmintics, as benzimidazoles and praziquantel, have limited effectiveness against these cestodiases, creating a demand for the identification of new and more effective drugs. Here, the potential of statins (simvastatin and fluvastatin), for repositioning as novel anthelmintic is explored. Statins are inhibitors of 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase, a main enzyme in the mevalonate pathway, which is vital for the synthesis of non-steroidal isoprenoids and the maintenance of normal cell functioning. A survey for HMG-CoA reductase encoding genes showed that they are present in a single copy in the genomes of parasitic helminths and their mammal hosts. Sequence alignments and phylogenetic analyses showed 20–95% overall identities among ortholog HMG-CoA reductases, with special conservation of their catalytic domains. The HMG-CoA reductase 3D-structure was predicted for orthologs from 3 cestodes of medical importance (Echinococcus multilocularis, Echinococcus granulosus sensu lato, and Taenia solium), and from a model cestode species (Mesocestoides corti). Molecular docking between cestode HMG-CoA reductase orthologs with simvastatin demonstrated that the Arg, Ser, Lys, and Glu residues in conserved positions of the active site interact with this drug, similarly to the interactions predicted for the human reference ortholog enzyme. Furthermore, in vitro assays demonstrated that simvastatin produced a significant reduction of M. corti viability, being able to reduce 100% of parasite viability at 150 μm. Fluvastatin was also assessed showing a lower, although significant anthelmintic effect. The predicted overall structures and interactions together with in vitro assays suggest that cestodes HMG-CoA reductases are inhibited by simvastatin, being a potential therapeutic target for the repurposing of simvastatin as anthelmintic drug. Furthermore, these results pave the way for the in vivo evaluation of the potential effects of simvastatin on cestode larvae.
This study investigated the role of temperament in oral language development in over 200 Mandarin and Cantonese speakers in the Growing Up in New Zealand pre-birth longitudinal cohort study. Mothers assessed infant temperament at nine months using a five-factor Infant Behaviour Questionnaire-Revised Very Short Form. They also reported on children’s vocabulary and word combinations at age two using adapted MacArthur-Bates Communicative Development Inventory short forms. Regression analyses were employed to examine unique links between infant temperament and language, respectively, controlling for demographic factors. Fear was associated with larger English vocabularies for English-Mandarin speakers and larger Cantonese vocabularies for Cantonese speakers. Orienting capacity was associated with more advanced word combinations for Mandarin speakers, whereas negative emotionality was associated with less advanced word combinations for Cantonese speakers. Positive affect/surgency was associated with more advanced word combinations for English-Cantonese speakers. This study revealed predictive patterns of infant temperament across Chinese-speaking children’s multiple languages.
Gas furnaces are the prevalent heating systems in Europe, but efforts to decarbonize the energy sector advocate for their replacement with heat pumps. However, this transition poses challenges for power grids due to increased electricity consumption. Estimating this consumption relies on the seasonal performance factor (SPF) of heat pumps, a metric that is complex to model and hard to measure accurately. We propose using an unpaired dataset of smart meter data at the building level to model the heat consumption and the SPF. We compare the distributions of the annual gas and heat pump electricity consumption by applying either the Jensen–Shannon Divergence or the Kolmogorov–Smirnov test. Through evaluation of a real-world dataset, we prove the ability of the methodology to predict the electricity consumption of future heat pumps replacing existing gas furnaces with a focus on single- and two-family buildings. Our results indicate anticipated SPFs ranging between 2.8 and 3.4, based on the Kolmogorov–Smirnov test. However, it is essential to note that the analysis reveals challenges associated with interpreting results when there are single-sided shifts in the input data, such as those induced by external factors like the European gas crisis in 2022. In summary, this extended version of a conference paper shows the viability of utilizing smart meter data to model heat consumption and seasonal performance factor for future retrofitted heat pumps.
We study the $\mathscr {D}\mathrm {isc}$-structure space $S^{\mathscr {D}\mathrm {isc}}_\partial (M)$ of a compact smooth manifold M. Informally speaking, this space measures the difference between M, together with its diffeomorphisms, and the diagram of ordered framed configuration spaces of M with point-forgetting and point-splitting maps between them, together with its derived automorphisms. As the main results, we show that in high dimensions, the $\mathscr {D}\mathrm {isc}$-structure space a) only depends on the tangential $2$-type of M, b) is an infinite loop space, and c) is nontrivial as long as M is spin. The proofs involve intermediate results that may be of independent interest, including an enhancement of embedding calculus to the level of bordism categories, results on the behaviour of derived mapping spaces between operads under rationalisation, and an answer to a question of Dwyer and Hess in that we show that the map $\mathrm {BTop}(d)\rightarrow \mathrm {BAut}(E_d)$ is an equivalence if and only if d is at most $2$.
Prandtl's secondary flows of the second kind generated by laterally varying roughness are studied using the linearised Reynolds-averaged Navier–Stokes approach proposed by Zampino et al. (J. Fluid Mech., vol. 944, 2022, p. A4). The momentum equations are coupled to the Spalart–Allmaras model while the roughness is captured by adapting established strategies for homogeneous roughness to heterogeneous surfaces. Linearisation of the governing equations yields a framework that enables a rapid exploration of the parameter space associated with heterogeneous surfaces, in the limiting case of small spanwise variations of the roughness properties. Channel flow is considered, with longitudinal high- and low-roughness strips arranged symmetrically. By varying the strip width, it is found that linear mechanisms play a dominant role in determining the size and intensity of secondary flows. In this setting, secondary flows may be interpreted as the time-averaged output response of the turbulent mean flow subjected to a steady forcing produced by the wall heterogeneity. In fact, the linear model predicts that secondary flows are most intense when the strip width is about 0.7 times the half-channel height, in excellent agreement with available data. Furthermore, a unified framework to analyse combinations of heterogeneous roughness properties and laterally varying topographies, common in applications, is discussed. Noting that the framework assumes small spanwise variations of the surface properties, two separate secondary-flow-inducing source mechanisms are identified, i.e. the lateral variation of the virtual origin from which the turbulent structure develops and the lateral variation of the streamwise velocity slip, capturing the acceleration/deceleration perceived by the bulk flow over troughs and crests of non-planar topographies.
The aim of this study was to analyse the validity and reliability of the Turkish version of the renal inpatient nutrition screening tool (Renal iNUT) for haemodialysis patients. The Renal iNUT and the malnutrition universal screening tool (MUST) were used in adult haemodialysis patients at two different centres to identify malnutrition. The subjective global assessment (SGA), regarded as the gold standard for nutritional status assessment, was utilised for comparison. Structural validity was assessed using biochemical values and anthropometric measurements, while reliability was assessed using repeated the Renal iNUT assessment. Of the 260 patients admitted, 42·3 % were malnourished (SGA score was B or C). According to the Renal iNUT, 59·6 % of the patients were at increased risk for malnutrition (score ≥ 1) and 3·8 % required referral to a dietitian (score ≥ 2). According to the MUST, 13·1 % of the patients were at increased risk for malnutrition and 8·5 % required referral to a dietitian. The Renal iNUT was found to be more sensitive in detecting increased risk of malnutrition in haemodialysis patients compared with the MUST (59·6 % v. 13·1 %). According to the SGA, the sensitivity of the Renal iNUT is higher compared to the MUST (89 % and 45 %, respectively). Kappa-assessed reliability of the Renal iNUT was 0·48 (95 % CI, 0·58, 0·9) and a moderate concordance was observed. The Renal iNUT is a valid and reliable nutritional screening tool for evaluating haemodialysis patients to determine their nutritional status. The use of the Renal iNUT by dietitians will contribute to the identification of malnutrition and its treatment.
Previous research has found that individuals harboring hostile sexist attitudes are more likely to support the use of political violence. In this study, we examine this relationship further. We theorize that the impact of hostile sexism on support for political violence is mediated through two mutually reinforcing factors: social dominance orientation and political illiberalism. We test this argument using an original survey we administered to over 1,400 subjects in the United States. We employ two operationalizations of individuals’ support for political violence: support in the abstract and support for specific acts of political violence. We find that individuals who exhibit hostile sexism are substantially more likely to support political violence, both abstract and specific. Moreover, we find that both social dominance orientation and political illiberalism together mediate 64.9% of the effect of hostile sexism on support for political violence in the abstract and 80.5% of the effect on support for specific acts of political violence. We conclude with a discussion of the implications of these findings.
Digital multimodal composing (DMC) has been valued as an engaging pedagogy in language teaching and learning in recent decades. Although research on DMC is flourishing and evidences its benefits for students' development as second language (L2) users and writers, there are some missing links between research findings and classroom practices. In this article, we examine three kinds of relationships between research and practice with regard to DMC: areas in which research findings have not been well applied, areas in which research findings have been reasonably well applied, and areas in which research findings have been usefully applied. As recent research–practice frameworks in education research emphasize a collaborative relationship between researchers and practitioners, we argue that L2 writing researchers' and teacher educators' reflections and experiences are crucial to facilitate the dialogue between DMC research and practice in writing contexts. We suggest that DMC should be incorporated into L2 teacher education programs so that instructors are equipped with the necessary knowledge and competence to design, implement, and assess students' DMC productions.
When considering the implications of the shareholder-stakeholder debate in defining the purpose of a company, epistemological clarity is vital in this emerging theory of the firm. Such clarity can prevent recurrence based solely on rephrasing key terms. To understand how various stakeholders develop and interpret a shared purpose, I argue for the necessity of a pragmatist approach that is normative and process-oriented. Mental models play a crucial role in interpretive processes that define decision-making, where individual perspectives converge. The figures of Milton Friedman and Ed Freeman serve as “beacons,” as artefacts, in the transmission of knowledge through which we, as individuals, shape a shared understanding. In current societies, profound polarization obstructs solutions to grand challenges. Pragmatism starts by questioning the underlying values of everyone involved. It assumes that sound deliberative processes are the only way to reach real solutions—not only for the mind but, above all, for the heart.