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A caseinomacropeptide (CMP) concentrate was produced from sweet whey by ultrafiltration (UF) and diafiltration (DF) using polyethersulfone (PES) membrane. Effects of the pH of whey feed and molecular weight cut-off of membrane (9 and 25 kDa) on permeate flux, recovery and purity of CMP were investigated. Before the UF, a pre-heat treatment at 90°C for 1 h and then acifidication to pH of 5 were applied to precipitate the major proteins in sweet whey. The pH value of UF feed was re-adjusted to different pH values (3, 4, 5, 7 and 9) to concentrate CMP molecule in the retentate and separate other whey proteins through permeate. Feed pH of 9 and 7 provided an adequate flux with 9 kDa- and 25 kDa-membrane, respectively. A volumetric concentration factor of 4 was reached with both membranes by UF and subsequent DF, but the process time was shorter with 25 kDa-membrane because of the higher permeate flux. One DF cycle was possible with 25 kDa-membrane as there was a substantial loss of CMP compared to four DF cycles with 9 kDa-membrane. About 58% of CMP in whey was recovered using 9 kDa-membrane while 33% of it was recovered with 25 kDa-membrane by the whole process. α-Lactalbumin, β-lactoglobulin, tyrosine and phenylalanine contents in the final concentrate, which are related to the purity of CMP were found similar in both cases. Sweet whey pre-treatment was the key point for purity of CMP concentrate before UF/DF. Both PES membranes can be used for the production of a CMP concentrate depending on desired recovery, composition and process time.
Clostridioides difficile is the primary cause of healthcare-associated infectious diarrhea in hospitalized patients. The most common laboratory testing methods for C. difficile infection (CDI) are toxin detection via enzyme immunoassay (EIA) and polymerase chain reaction (PCR), which detect a toxogenic strain. This study examines the impact of Rhode Island’s largest hospital system changing from PCR-only to two-step CDI testing.
Methods:
A retrospective cohort study of 2,173 adult inpatients was conducted. Patients were grouped into two cohorts: those tested for toxigenic C. difficile via PCR-only (June 2019–May 2021, n = 1,194) and those tested with the two-step algorithm (June 2021–May 2023, n = 979). Cluster analysis identified patient risk groups for hypothesis generation, and complications such as death, colectomy, intensive care unit ICU transfer, and 30-day readmission were compared across these groups.
Results:
In the moderate-risk group, there was a significant reduction in ICU transfers and readmission rates with the two-step testing by 5% and 7%, respectively. There were no other significant differences in complications between testing groups. Anti-CDI antibiotics were discontinued in 15% (n = 106) of EIA-negative patients in the two-step testing group. Moderate-risk patients were less likely to have treatment discontinued than severe-risk patients (OR = 2.00, p = 0.016).
Discussion:
The two-step testing algorithm did not negatively affect patient outcomes and led to a modest decrease in anti-CDI treatment, supporting the safety of two-step CDI testing in hospitalized patients.
It is assumed that the biology and ecology of commercial fish species are relatively well-known, given that many of these parameters are key for stock assessment in fisheries management. Surprisingly (or not), several new parasite species are described annually from fish that are of commercial and cultural importance. Haddock (Melanogrammus aeglefinus) is an important commercial fish species in the North Atlantic with more than 50 parasite species having been reported from it. Despite its commercial importance in Icelandic waters, only 11 parasite species have been reported from Icelandic haddock. In February and March 2023, 26 haddock were sampled, including 16 and 10 from the north and south of Iceland, respectively. Fish were examined for parasites, with a focus on macroparasites (large, usually visible to the eye). Parasites were identified morphologically with identifications of helminths confirmed using DNA barcoding (Sanger sequencing). Overall, 19 different parasite species were recovered with 17 being shared between haddock sampled from the north and south of Iceland. Of these, eight represent new geographical records for parasites of haddock in Icelandic waters. Our study indicates that monitoring for parasites remains important, regardless of how well a species has been studied. Furthermore, reporting parasites per organ and per region, especially when areas are known to be influenced by different abiotic and physical features, is important in the context of parasites as biological tags for stock identification. Despite a small sample size, our study suggests that some parasites might act as potential biological tags for stock identification of haddock in Icelandic waters.
Digital technologies have transformed the way governments around the world maintain social and political order. However, the intrusive and often repressive nature of modern political control mechanisms, such as digital surveillance and digital censorship, is largely concealed from the public and becomes “normalized” by state propaganda, particularly in authoritarian regimes. Engaging with the political psychology literature on emotion, we examine how citizens respond emotionally to such control when exposed to relevant revealing information and how these emotions relate to shifts in attitudes toward authoritarian governments. Using a survey experiment and 50 in-depth interviews conducted in China, we find that exposure to revealing information about digital control slightly amplifies negative emotions but profoundly reduces positive emotions and significantly undermines public support for authoritarian digital governance. These effects are more pronounced in the context of digital surveillance than censorship and are most severe when individuals perceive control measures as personally targeted. Our findings underscore the political-psychological consequences of digital control, emphasizing the role of emotions in shaping public responses to digital authoritarianism based on new insights into the affective dimensions of digital repression.
Previous research on the underpinnings of support for conspiracy theories has explored the role of both generalized trust and political trust, but scholars have yet to explore whether both generalized and political anxiety are related to support for conspiracy theories. While studies have shown that general measures of anxiety predict support for conspiracy theories, researchers have not yet devoted much attention to understanding whether feelings of political anxiety are also related to conspiracy theory endorsement. Using data from an original survey fielded in 2023 that includes a measure of generalized anxiety and an eight-item measure that specifically captures political anxiety, we find that political anxiety is not related to conspiracy theory support, and that generalized anxiety is weakly related to conspiracy theory support. Thus, although there are good reasons to be concerned about the ill effects of political anxiety, it does not appear to be strongly related to conspiracy theory endorsement.
We use particle-based simulation to study the rheology of dense suspensions comprising mixtures of small colloids and larger grains subject to contact, lubrication and Brownian forces. These suspensions exhibit shear thinning at low shear rates and shear thickening at high shear rates. By systematically varying the volume fraction of the two species, we demonstrate a monotonic increase in viscosity when grains are added to colloids, but, conversely, a non-monotonic response in both the viscosity and shear-thickening onset when colloids are added to grains. Both effects are most prominent at intermediate shear rates where diffusion and convection play similar roles in the dynamics. We rationalise these results by measuring the maximum flowable volume fraction as functions of the Péclet number and composition, showing that in extreme cases increasing the solids content can disrupt grain contacts and thus allow a jammed suspension to flow. These results establish a constitutive description for the rheology of bidisperse suspensions across the colloidal-to-granular transition, with implications for flow prediction and control in multicomponent particulate systems.
Authenticity has been a central concept in sociolinguistics and in the study of literary representations of dialect. This article examines the ideology of dialect authenticity in the context of literary fiction from the point of view of language users. Two Finnish reading groups comprising members with different dialect backgrounds read one Finnish novel, in which the Far Northern dialects of Finnish are represented in a partly unconventional manner. Thematic analysis was applied to two video-recorded reading group discussions to investigate how the groups discuss the novel’s dialect representation and its (in)authenticity. The analysis revealed that instead of adhering to a static and essentialist ideal of authenticity, the readers overlooked the unconventional representation of literary dialect and viewed authenticity as a dynamic process. The study contributes to theoretical discussions on dialect authenticity and employs an experimental approach to exploring language ideologies through reading groups.
This article explores the patriotic war bonds of the Second Polish Republic, which were sold both domestically and to the diaspora in the United States, during Poland’s border conflicts of 1918–21. It reveals how government and grassroots initiatives converged to mobilise diverse constituencies in Poland, including ethnic minorities, women and the poor. In the United States, these efforts were also marked by tensions between patriotism and professionalism in fundraising for a nationalist cause. It finds commonalities between the Polish war bonds and those of the Great War, in particular the US Liberty Loans. However, for Poland, the article argues, the bonds were not merely financial instruments but also vehicles through which the Second Polish Republic performed its newfound sovereignty, seeking popular recognition at home and abroad.
Fear of cancer recurrence (FCR), defined as worry about cancer progression or return, is a common concern among patients and survivors. This study aims to identify factors influencing the onset of FCR and to evaluate its impact on emotional distress and quality of life (QoL) in patients undergoing systemic cancer treatment.
Methods
A sample of 175 patients undergoing systemic cancer treatment completed self-report questionnaires assessing socio-demographics, clinical factors and fear of cancer recurrence, anxiety, depression and QoL (FCRI, HADS, EORTC-QLQ-C30).
Results
Data analysis using linear and generalized linear models revealed that FCR is inversely associated with QoL and positively correlated with anxiety and depression. These associations were independent of socio-demographic and clinical variables, with anxiety and depression fully mediating the FCR-QoL relationship. Gender and time since diagnosis emerged as significant predictors, with an increase in FCR at 12 months post-diagnosis.
Significance of the results
Our results indicate that FCR, anxiety and depression linked, are present after several months post-diagnosis, suggesting that FCR tends to intensify over time. These findings carry important psychological implications, highlighting the need to support patients in recognizing and managing their fear and emotional distress. Implementing a psychoeducational counseling approximately six months after diagnosis – engaging oncologists, psycho-oncologists, and patients within a “stepped-care” framework – may be effective in mitigating FCR and its emotional consequences.
Poikilothermic and short-lived drifter species such as zooplankton are highly responsive to climate-driven changes and frontal systems in the Southern Ocean. To understand these changes, ongoing assessment of zooplankton is essential. Changes in abundance, biomass and species composition of zooplankton in the Indian sector of the Southern Ocean were assessed during the austral summer of 2022/2023 using a ring net that was towed obliquely from an average depth of 200 m to the surface. Thirty-six zooplankton taxa were identified at seven stations, with copepods constituting 64% of the population across 23 species. Zooplankton abundance and biomass were higher in Transect 2 (60–64°S) compared to Transect 1 (50–54°S). A cluster analysis revealed four zooplankton community groups. Notably, the distribution patterns of non-copepod taxa appeared to be influenced by diel vertical migration behaviour, which was closely tied to seasonal dynamics and the timing of sampling. This study improves our understanding of how different oceanographical fronts and behavioural patterns shape zooplankton communities in the Southern Ocean, emphasizing the need for improved sampling to include smaller zooplankton species and to better resolve temporal dynamics.
In ADHD a common obstacle of academic success is impaired reading comprehension. Impaired comprehension in ADHD is accompanied by altered eye movements during reading as well as more general eye movement deficits associated with non-verbal stimuli. This suggests that the reading deficits do not cause the eye movement impairment. Instead, eye movements might contribute to reading comprehension difficulties.
Methods:
We tested whether minimizing the need for eye movements during reading aids comprehension. We measured reading comprehension in a sample of undergraduate students with and without ADHD. Students read short paragraphs using normal text reading with all words fully visible (FULL), PACED reading that preserved text layout with one word at a time appearing at its usual location in the text, and reading with minimal eye movements in which one word at a time appeared in the center of the screen in a rapid serial visual presentation (RSVP).
Results:
ADHD participants performed better in the RSVP condition relative to the other two reading conditions that required eye movements, and they benefited from the RSVP condition requiring minimal eye movements by almost 13% relative to neurotypical controls, who showed comprehension difficulties using the RSVP mode.
Conclusions:
Minimizing eye movement boosted reading comprehension in the ADHD suggesting that eye movements are implicated in reading processes in ADHD, an interference that can be avoided in the RSVP reading condition. Future work should explore the possibility of RSVP as a reading aid in ADHD adults and potentially school-aged children.
Violent variability, such as flaring activity, of planet-hosting stars is known to strongly affect prebiotic processes on their planets; therefore, its role in such processes is being extensively studied nowadays. On the contrary, the role of regular variability, such as variability of pulsating stars, has yet been unexplored. In this article, we investigate how large-amplitude variability of pulsating stars may affect the prebiotic evolution on their planets. We show that the RR Lyr type variables are particularly relevant because their lightcurves possess amplitudes, periods, and time profiles that are potentially most suitable to drive prebiotic reactions.
This study examines the elicited production of Spanish infinitives versus gerunds among Spanish/English bilingual children and adolescents in the United States. We focus on three contexts: infinitives in subject position, infinitives with the phrasal verb parar de (“to stop doing something”), and infinitives with the prepositional verb parar a (“to stop to do something”). Results showed that children and adolescents produced fewer infinitives than their Spanish-dominant parents in subject position and with parar de, often overextending the gerund. By contrast, all groups performed more accurately with parar a, where English and Spanish align structurally. Language dominance and Spanish experience significantly predicted more target-like infinitive use, while chronological age and English dominance were associated with increased gerund overextension. These findings support the Bilingual Alignment Hypothesis, showing that heritage Spanish morphosyntactic development is gradual and context-sensitive, with greater accuracy in areas of crosslinguistic convergence.
Use-wear analysis is rarely conducted for ground stone axes (GSAs) from West Africa. Here, the results of use-wear analysis of 50 GSAs from Akwanga and other parts of Central Nigeria are discussed, contributing to our understanding of their functional attributes.
With this paper, we aim to bring the history of the rural landscapes and communities of the ancient (‘Classical’) Mediterranean back into the limelight, drawing attention to their contributions to and pivotal roles within the multifaceted structural transformations of the Mediterranean in the first millennium bce. To do so, we focus on two case studies from one particular region that looms large amongst those heavily exploited by ancient colonial powers: the island of Sardinia. In chronological terms, our focus is on the so-called Punic and Roman periods, roughly spanning between the fifth century bce and the fifth century ce. Long overlooked, if not outright dismissed, in conventional accounts of the ancient Mediterranean, the rural communities of Punic-Roman Sardinia were not only vital economic producers, but also formed large and culturally distinct social groups. They actively maintained their own traditions, ways of living and practices in the face of the ruling classes’ disruptive initiatives. Their actions to shape their identity and history resonate closely with the theory of the ‘history of subaltern groups’ formulated in Antonio Gramsci’s Prison Notebooks, particularly Notebook 25. We draw upon a semiotic understanding of Gramsci’s notion of subalternity to strengthen archaeology’s ability to foreground the materiality of those communities unaccounted for by history. Our goal is to discuss comparatively the material signs of rural life of Punic and Roman-period Sardinia, to outline an alternative decolonial perspective on the island and to consider its implications for the wider ancient Mediterranean.
The presence of insects in hospital environments poses a potential risk for the dissemination of pathogenic bacteria, including multidrug-resistant species. Despite strict sanitation protocols, some arthropod populations persist in less regulated areas, potentially acting as mechanical vectors of bacterial contamination.
Objectives:
This study aimed to analyze the bacterial diversity associated with insects collected in hospital settings and assess their potential role in spreading pathogens relevant to public health.
Methods:
A descriptive observational approach was employed to identify and classify bacterial taxa associated with hospital-collected insects. High-throughput sequencing was used for taxonomic classification at the phylum, family, and genus levels.
Results:
Proteobacteria was the predominant phylum, additionally we found families such as Moraxellaceae and Mycobacteriaceae, known to include clinically relevant species. The genera Acinetobacter and Mycobacterium were particularly abundant in some samples, raising concerns about their potential role in nosocomial infections. Other identified bacteria included Pseudomonas, Escherichia, and Shigella, albeit at lower frequencies. The persistence of these bacteria in hospital environments suggests that insects may contribute to their dissemination.
Conclusions:
The findings highlight the need for enhanced arthropod control measures in healthcare facilities as part of routine biosecurity protocols. The presence of multidrug-resistant bacteria associated with hospital-dwelling insects reinforces their role in pathogen transmission, emphasizing the importance of comprehensive vector management strategies to mitigate nosocomial infection risks.
Three motor sledges were taken on Captain Robert Falcon Scott’s expedition to Antarctica in 1910. They performed poorly, making only small contributions to the polar journey and making no contribution to the expedition’s scientific programme.
The motor sledges have received little attention from historians and researchers. No definitive work has been published. The purpose of this article is to provide an authoritative, reliable and complete history of Scott’s Antarctic motor sledges.
This article studies Belton Hamilton’s concept for a “chain track” vehicle, then traces its development path through two prototype vehicles and two snow trials in Norway. The outcomes of the snow trials and associated recommendations are reviewed. The article then considers Scott’s detailed plans to reach the South Pole and his instructions to the Motor Party in pursuit of that goal. Four major problems that prevented the motor sledges from satisfying Scott’s instructions are identified.
Several conclusions are drawn. It is apparent that the vehicles were flawed from the outset by poor engineering decisions about track design, engine power and carburetion/airflow. It is unlikely that experimentation or minor refinement in the Antarctic would have produced vehicles reliable enough to make a major contribution to the polar journey.